Artificial Insemination Techniques Research Articles

Nerve Growth Factor (NGF) and Animal Reproduction.

Stimuli that lead to the release of gonadotropin-releasing hormone (GnRH) and pituitary gonadotropins and, consequently, ovulation in mammals fall into two broad categories. In the first, high plasma oestrogen concentrations induce the events that trigger ovulation, a characteristic of spontaneous ovulators. In the second, nerve stimuli occurring during mating reach the hypothalamus and trigger the release of GnRH and ovulation with a neuroendocrine reflex that characterizes induced ovulators.In this review, we will give an overview of the distribution of NGF and its expression in the different tissues of the male accessory sex glands, the main sites of NGF production. Next, we will highlight the role of NGF in sperm function and its potential cryopreserving role in artificial insemination techniques. Finally, we will evaluate the functions of NGF in ovulation, particularly in induced ovulators. Overall, the information obtained so far indicates that NGF is widely distributed in organs that regulate the reproductive activity, in both males and females. In spontaneous ovulators, NGF exerts mainly a luteotrophic action, while, in induced ovulators it is the main ovulation-inducing factor. A better understanding of the role of NGF in reproduction would be of great interest, since it could help finding innovative therapeutic aids to improve mammalian fertility.

Constraints Identification and on Station Evaluation of Hormonal Assisted Artificial Insemination on Reproductive Performance of Horro Cattle in Western Oromia, Ethiopia

The study was conducted in Guto gida, Diga and Sibu sire districts of East Wollega, Bako tibe and Ilu galan district of West Shaw zones and on station in Bako agricultural research center with the objectives to assess constraints associated with hormonal assisting artificial insemination and to evaluate the response of Horro cattle (Bos indicus) cows after estrous synchronization. In this study, structured questioner was used and a total of 204 respondents (180 small holder dairy farmers, 8 animal health workers, 8 animal production professionals and 8 AI technicians were interviewed. Retrospective data from specified districts were also included to identify constraints associated with hormonal assisting artificial insemination in the study areas. The study indicated that most of the respondents (93.33%) were got information/ took training on the issue of mass estrous synchronization and AI services before the commencement of the program. However, most of the respondents (57.78%) had moderate knowledge on heat detection and only few (27.22%) of them can keep the record of their synchronized cows/heifers. The main restraints for the low success rate of on farm hormone assisted artificial insemination (estrus synchronization) were identified as failure of conception (42.22%), using poorly managed cows/heifers (15%), difficulty in heat detection by the farmers (13.89%), skill gap and unavailability of artificial insemination technicians (AITs) (12.78%) and poor hormonal responses (11.11%) and the assessment made on farm in comparison with observational test made on station indicated that there were minimum or very few problems with estrus synchronization since majority of cows hormonally treated were conceived as that of normally cycling animals. Therefore, to enhance the genetic potential of indigenous dairy cows through cross breeding scheme, revising of the existing hormone assisted estrus synchronization, artificial insemination delivery system, improving the ability of farmers and experts on the handling of frozen semen and management of dairy cows/heifers before and after hormonal synchronization should be due in place. Moreover, awareness creation should be done to change the attitude of farmers on hormone assisted estrus synchronization.

Perspectives of intrauterine artificial insemination applicability in gilts

ABSTRACT: The application of the intrauterine artificial insemination (IUAI) technique allows optimization of a swine production system due to the reductions in volume and number of sperm cells in the insemination dose, and by reducing the time taken to perform the insemination. However, IUAI is not recommended for gilts due to the difficulty of intrauterine cannula passage through the cervix. This difficulty is associated mainly with the fact that the reproductive tract is smaller in gilts than in pluriparous females. However, few studies have evaluated the application of IUAI in gilts. In these studies, there are variations in approach concerning the definition of the success rate for cannula passage through the cervix, the type of cannula and the body characteristics of the gilts used, making it difficult to extrapolate the recommendation for the use of IUAI in gilts. Considering the evidence that such characteristics influence or even determine the success of the application of IUAI, there is a necessity for an understanding of the influence of these factors in the improvement and later application of the technique. Gilts represent about 15-20% of the breeding group, and the use of IUAI could optimize the processes of insemination on farms. The approach used in this review highlights the aspects that could aid in structuring further studies for improving IUAI in gilts, allowing its use on commercial farms.

Oestrus synchronization of West African Dwarf Does using prostaglandin-F2α analogue

Introduction: Successful livestock propagation through artificial insemination technique is achievable only when the different processes involved, including oestrus synchronization, are well executed. Methods: Dinoprost thromithamine (Lutalyse®) is an analogue of Prostaglandin F2-alpha (PGF2α) used extensively for oestrus synchronization in ruminants. Its efficacy as oestrus synchronizing agent was studied using six (6) healthy West African Dwarf (WAD) does aged between 2 and 4years and weighing 6-13 kg. The animals, randomly divided into three groups of 2 animals each, were kept in a standard goat pen and allowed to graze from morning to evening. They were fed with concentrates in the evening and supplied water ad libitum. The multiparous group (I) and nulliparous group (II) were administered two intramuscular injections of 5mg Lutalyse® at 11 days interval while the control group (III) was not given any treatment. The animals were closely monitored for signs of oestrus during and after the treatments. Oestrus detection was carried out by teasing the does twice daily (0800 and 1600 hours respectively) using one vasectomized buck per set of does. Results: The 2 does in group I responded to the treatment, while does in group II and III did not. Group I does were seen to come on heat between 48th and 96th hours after the second injection (100% response). The two does in Group I showed intense oestrus (90.00 %) while does in Group II presented weak oestrus. Significance: From this study, it can be concluded that 5mg of Dinoprost is effective as an agent of oestrus synchronization in multiparous WAD does.

Birth of a Live Cria After Transfer of a Vitrified-Warmed Alpaca (Vicugna pacos) Preimplantation Embryo.

The alpaca (Vicugna pacos) is an important species for the production of fiber and food. Genetic improvement programs for alpacas have been hindered, however, by the lack of field-practical techniques for artificial insemination and embryo transfer. In particular, successful techniques for the cryopreservation of alpaca preimplantation embryos have not been reported previously. The objective of this study was to develop a field-practical and efficacious technique for cryopreservation of alpaca preimplantation embryos using a modification of a vitrification protocol originally devised for horses and adapted for dromedary camels. Four naturally cycling non-superovulated Huacaya females serving as embryo donors were mated to males of proven fertility. Donors received 30 μg of gonadorelin at the time of breeding, and embryos were non-surgically recovered 7 days after mating. Recovered embryos (n = 4) were placed individually through a series of three vitrification solutions at 20°C (VS1: 1.4 M glycerol; VS2: 1.4 M glycerol + 3.6 M ethylene glycol; VS3: 3.4 M glycerol + 4.6 M ethylene glycol) before loading into an open-pulled straw (OPS) and plunging directly into liquid nitrogen for storage. At warming, each individual embryo was sequentially placed through warming solutions (WS1: 0.5 M galactose at 37°C; WS2: 0.25 M galactose at 20°C), and warmed embryos were incubated at 37°C in 5% CO2 in humidified air for 20–22 h in 1 ml Syngro® holding medium supplemented with 10% (v/v) alpaca serum to perform an initial in vitro assessment of post-warming viability. Embryos whose diameter increased during culture (n = 2) were transferred individually into synchronous recipients, whereas embryos that did not grow (n = 2) were transferred together into a single recipient to perform an in vivo assessment of post-warming viability. Initial pregnancy detection was performed ultrasonographically 29 days post-transfer when fetal heartbeat could be detected, and one of three recipients was pregnant (25% embryo survival rate). On November 13, 2019, the one pregnant recipient delivered what is believed to be the world's first cria produced from a vitrified-warmed alpaca embryo.

Application of laparoscopic artificial insemination in conventional Lacaune sheep farm using frozen-thawed semen

The complex anatomical structure of the ewe reproductive tract accompanied with low quality of frozen ram semen for artificial insemination, resulted in a challenge with regard to using superior genotypes for reproductive ovine performance. Hence, improved genetics in ovine management has not been efficiently and widely used especially in undeveloped countries. Therefore, intrauterine semen deposition by laparoscopic insemination should be adopted in the current sheep production systems. Thus, this study aimed to assess the pregnancy rate and lambing rate of ewe inseminated by laparoscopic insemination techniques using frozen-thawed semen. The research used imported frozen semen from two rams of the Lacaune breed. Ewes were grouped according to age in years (1, 2 and 4). Before insemination, the semen was examined microscopically for its motility and viability and thereafter the laparoscopic artificial insemination technique was performed to 19 Lacaune breed ewes using frozen-thawed semen. The overall pregnancy and prolificacy rates were 31.57% and 42.10% respectively. Out of 2 ewes in the 1-year age group that were inseminated, only 1 ewe lambed representing 50%. However, from 16 ewes inseminated of four-year age group, 5 ewes lambed representing 31.25%. Significant difference based on age group was not evaluated due disproportionate of the data, (such that the data included 2 ewes in one-year-old age, 1 ewe in 2-year-old age and 16 ewes in 4-year-old age). Based on the ram semen, 33.33% and 30% of the inseminated ewes were pregnant from ram A and ram B semen respectively. However, in the case of prolificacy rate, 44.44% and 40 % of the ewes lambed from using semen of ram A and B, respectively. There was no significant difference (p>0.05) in pregnancy and prolificacy rates based on semen from the two rams. In conclusion, in this research study, ram semen had no significant effect on pregnancy and prolificacy rates using laparoscopic AI on Lacaune sheep. This could be due to the fact that the rams had very good quality semen. Evaluation of ram semen, accompanied with appropriate ewe selection based on age and rightful deposition of semen could lead to better and more consistent results. Overall this could contribute to the successful application of laparoscopic artificial insemination in Lacaune sheep production systems for enhanced productivity.

71 Imitating the dynamic bovine cervix with 3D printing technology to teach artificial insemination in cattle

Abstract The process of artificial insemination in cattle is commonly taught using excised reproductive tracts. Excised reproductive tracts can be difficult to collect, and often require freezing and thawing prior to use; however, they are an effective tool to teach artificial insemination (AI) techniques. As an alternative tool, 3D printed cervixes were created using NinjaFlex filament in a Flashforge Creator Pro Dual Extrusion Printer. Designs of cervixes were created with a 3D CAD software while MATLAB was used to generate the random placement of the cervical ring openings to model biological diversity. The objective was to determine the effectiveness of using a 3D printed cervix compared with an excised reproductive tract to teach AI in cattle. Data were collected via surveys for 120 students from 2016 through 2019. Students were divided in two groups prior to entering live animals: 1) Excised reproductive tract and 2) 3D Cervix. The excised reproductive tracts were previously collected, preserved, and confirmed passable by instructors. The 3D cervix group included 3D cervixes mounted in a bovine pelvis inside a box. Overall, 88% of students had success passing the cervix in the cow by the third day. Eighty-seven percent of students thought the excised tracts felt somewhat similar to a live cow, compared with 54% who thought the 3D cervixes alone felt similar to a live cow. When the 3D cervixes were mounted in the box, 17/20 students thought they felt similar to a live cow. For learning AI techniques, 12/21 students thought the excised tracts were more helpful than the 3D cervixes alone (5/21); however, when the 3D cervixes were mounted in the pelvic boxes, 11/21 students preferred this setup compared with the excised tracts (6/21 students). Student feedback suggests the 3D cervixes mounted in pelvic boxes are a useful tool for teaching artificial insemination techniques.

Artificial Insemination Training Program for Smallholder Pig Farms in Gauteng Province, South Africa

The aim of this study was to facilitate artificial insemination training to enhance sustainable pig production within the developing smallholder pig production sector in Gauteng Province, South Africa. Eighteen smallholder pig farmers with requisite structures (pig house, pens), pigs (large white, landrace duroc or South African indigenous) and management (feeding, cleaning and record keeping) capacity were trained on routine pig management and artificial insemination procedures in a “learning by doing” on-farm supervised programme administered by Agricultural Research Council, Animal Production pig training team. Following estrus detection, 96 sows were artificially inseminated and 31 naturally served (NS). Farrowing rates (FR), total born (TB) and born alive (BA) piglets were recorded. The occurrence ccurrence of mummified fetuses (0.019 vs. 0.022%) and weak piglets (0.038 vs. 0.049%) did not differ between artificially inseminated sows and naturally mated sows. Born alive, birth weight and weaning weight were higher for artificial inseminated sows. The average litter size was 15± and 13±, birth weight 1.98±0.79 kg and 1.48±0.58 kg and weaning weight 9.89±0.87 kg and 7.23±0.71 kg for the AI and NS litters, respectively. Farmer demographic factors (age, gender and educational level) had no effect on farrowing rate, total born and piglets born alive. Therefore, implementation of artificial insemination techniques and pig production training was feasible under a smallholder pig production system.

Histología de la cervix de oveja Santa Inês sometida a la técnica de inseminación por retracción cervical

This trial aimed to evaluate the technique of artificial insemination by cervical retraction (CRI) in the histological parameters of the cervix of Santa Inês sheep. Three pluriparous cyclical ewes were used, with four years old, live weight 54.30 ± 1.20 kg and body condition score 3.0, at slaughter. At the time of estrus, two ewes were subjected to the insemination technique by cervical retraction (CRI). Three different moments were considered for the slaughter of the ewes: “0h”, represented by the non-CRI ewe; “24h” and “72h” after CRI technique, slaughtering the two ewes at these respective times. The cervix were collected and prepared for histological evaluation. Histological sections showed tissue integrity at the time of estrus (0h, without CRI) and an intense inflammatory reaction 24 hours after CRI, with the presence of hemorrhage, edema and cell degeneration. However, it was observed that this inflammatory condition was attenuated, with tissue reorganization within 72 hours.

Efficiency of Different Diluents and Dilution Rates on the Fertilization Potential of Chicken Spermatozoa

Background: Artificial Insemination (AI) has been well established to obtain better fertility than natural mating. Semen extension and preservation play a major role in AI technique. The concentrated nature of chicken semen entails dilution of semen for easy handling and storage. With the availability of a wide range of extenders, exploring various extenders and dilution rates supplement the reproductive efficiency achieved through modern AI techniques.Methods: Pooled semen samples obtained from twenty White Leghorn breeder males were split equally into three portions, each for CARI poultry semen diluent, EK extender and Tselutin extender. At 1:2 dilution rate, the samples were subjected to sperm motility analysis from 0 to 96 h and AI was targeted at 0 and 24 h of semen collection. Fertility was assessed by candling of eggs collected from 2 to 8 days post insemination at 9th day of incubation. Further investigation of different dilution rates (1:2, 1:4, 1:6, 1:8, 1:10, 1:12, 1:14, 1:16, 1:18, 1:20) on fertility of fresh semen was done using the superior diluent.Result: Upon analysis, sperm motility showed no significant difference among the diluents at 0 h storage while CARI poultry semen diluent showed superior motility (%) at the subsequent storage periods, followed by EK and Tselutin extender. Higher fertility (p less than 0.05) was expressed in the diluents of CARI and EK at 0 h storage, whereas CARI poultry semen diluent showed superior fertility pattern (p less than 0.05) followed by EK and least by Tselutin extender at 24 h semen storage. Further investigation of different dilution rates using CARI diluent, exhibited fertility (p less than 0.05) of 90% and higher at 1:2 and 1:4 dilutions, followed by 1:6 and 1:8 at 2 to 6 days of fertile period.

Reproductive performance in gilts following applications of different insemination doses and techniques

This study evaluated the applicability of intrauterine artificial insemination (IUAI) in gilts and the impact of age at insemination and different body characteristics of gilts on the success rate for cannula insertion. Additionally, reproductive performance was evaluated for IUAI and cervical artificial insemination (CAI), considering different semen dose sizes. A total of 636 gilts were assigned in a 2 × 2 factorial design: two artificial insemination techniques (CAI and IUAI) and two semen dose sizes (1.5 × 109 sperm cells/50 mL or 2.5 × 109 sperm cells/80 mL). In those gilts assigned to IUAI (n = 319) the success rate for intrauterine cannula insertion was evaluated according to weight at first detected estrus, body condition score (BCS), and age at insemination. Reproductive performance, occurrence of bleeding, and semen backflow during all inseminations were compared among groups (2 × 2). Two subgroups were evaluated regarding the time expended to perform insemination (n = 380), and the semen backflow collected during 1 h after insemination (n = 114). The success rate for intrauterine cannula insertion, based on a successful insertion in all inseminations performed during estrus, was 58.9%. Additionally, greater possibility (>60%; P ≤ 0.04) of cannula insertion was observed in heavier gilts (≥124 kg), as well as in older gilts (≥225 d) and those with greater BCS (>3). There were no differences among the groups in pregnancy rate (≥95.3%; P = 0.23), farrowing rate (≥93.7%; P = 0.54), total piglets born (≥14.5; P = 0.45), as well as, bleeding (P = 0.48) and backflow (P = 0.48) during insemination. However, the percentage of semen backflow volume and percentage of sperm cells in the backflow were lower in gilts inseminated by CAI with 1.5 billion sperm cells/50 mL (P < 0.01) than in the other groups. There was no expressive reduction in time expended to perform IUAI compared to CAI. However, gilts inseminated with 1.5 billion sperm cells/50 mL showed a lower total time to inseminate than all other groups (P < 0.01). In conclusion, higher weight, BCS, and age increased the success rate for cannula insertion. However, IUAI did not optimize the insemination procedure, and remains limited for gilts due to the low success rate for cannula insertion. Reproductive performance was not affected by IUAI or CAI using 1.5 or 2.5 billion sperm cells in 50 or 80 mL, respectively, suggesting the possibility of using CAI with 1.5 billion sperm cells/50 mL in gilts.

English

The aim of the survey was to assess artificial insemination (AI) service delivery system and identify the challenges and opportunities of AI service in cattle production in Western Zone of Tigray Region, Ethiopia. Twenty Kebeles (lowest administrative units) and 353 respondents were purposively involved in face-to-face interview. Data analysis was performed using SPSS and mainly summarized by frequency and percentages. Chi-square test was the statistical method used to test proportions of variables. There was statistically significant (P<0.05) differences in AI service interruptions in both regular working hours and weekends and holiday hours. AI service interruption was a critical challenge in regular working days and in weekends and holidays. This is because 73% of the respondents reported that AI service was interrupted in Monday through Friday and 75% of the respondents also reported that Artificial Insemination Technicians (AITs) were not available to deliver AI service in weekends and holidays. Above half (63%) of the small scale farmers reported that AITs were very poor. Most of the very poor AITs were in midland (48%) and lowland (36%) agro-ecologies compared to the AITs in highland (16%) agro-ecology. Mobile AI service delivery system through motor bikes was the most common AI service delivery system used. The major challenges of AI service in order of their importance were lack of community awareness, poor animal management, unskilled AITs and inadequacy of AITs. The government and NGOs should solve the current challenges of AI service. The current approach and system of AI delivery should be restructured. Adequate numbers of skilled manpower should be assigned based on the standard numbers of breedable cows per AI center or per AIT. Moreover, agro-ecology based human management system and infrastructure development should be adequately addressed. Key words: Communication means, AI service interruption, distance between homestead and AI service center, AI service delivery system, agro-ecology.

Extenders for alpaca epididymal spermatozoa: Comparison of INRA96 and andromed

Artificial insemination would be a useful technique for alpaca breeders to use as an aid to breeding to increase fleece quality. The technique, however, is not well developed in alpacas, partly because of the viscous nature of their seminal plasma. Castration conducted for husbandry purposes can provide a source of epididymal spermatozoa to test semen extenders or handling regimens, thus circumventing the problem of the viscous ejaculate. In this experiment, two semen extenders (Andromed and INRA96) developed for other species (bovine and equine, respectively) were tested with alpaca spermatozoa derived from the cauda epididymis. Sperm total motility (mean ± SEM A: 29.1 ± 4.8 % compared with I: 35.4 ± 4.8 %; NS), membrane integrity (A: 58 ± 9% compared with I: 56 ± 9%; NS) and acrosome integrity (A: 65 ± 7% compared with I: 54 ± 7%; NS) were not different between the two extenders. Progressive motility with use of INRA96 was greater after incubating for 30 min than after incubating for 10 min (35 ± 4% vs. 12 ± 4%, respectively; P = 0.03). In conclusion, viable epididymal spermatozoa could be extracted from the castrated organs after overnight transport. There were no differences in sperm quality between the two extenders; therefore, it appears that either extender could be used for alpaca spermatozoa. These results could help in the development of a technique for artificial insemination in alpacas.

Advances in gender preselection in swine

Gender preselection using isolated populations of X- and Y-chromosome bearing spermatozoa that have been separated on the basis of DNA content is currently possible in swine and other farm animals, as well as in humans. Semen from most livestock species can now be successfully separated into predominantly X or Y sperm populations before their use for intra-tubal insemination, deep-uterine insemination or for in vitro fertilization (IVF) to produce sexed offspring. Birth of progeny of the desired sex in cattle, sheep and swine under semi-practical conditions has successfully validated the sexing technology. Spermatozoa are separated on the basis of inherent differences in DNA content in the X- and Y-chromosome bearing sperm population using modified flow cytometry /cell sorting technology. Spermatozoa are stained with Hoechst 33342 which binds to the DNA in an amount proportional to the amount of DNA present in the individual spermatozoa. Over 300 animals from various species at several locations have been born using the USDA-Beltsville Sperm Sexing Technology for separating X and Y spermatozoa. Sex ratios are shifted from the normal 50:50 to 85 to 90% of one sex or the other. In swine, offspring have been born as the result of surgical intratubal insemination of separated spermatozoa and also from 11/F and embryo transfer. At the present time, standard swine artificial insemination techniques are not optimized for use with the small numbers of flow cytometrically separated X or Y sperm populations. Cattle, swine and rabbit offspring have been reproduced through the second generation with normal morphology and reproductive function. Numerous improvements have been made in the sexing technology since it was first reported in 1989. Increasing the speed of the sexing process to make the application of the technology available to a larger segment of the livestock industry is paramount, even with insemination technology designed for small numbers of spermatozoa.

Information needs of paravets on artificial insemination in India

The present study was conducted to assess the information needs of the paravets on Artificial Insemination (AI) in India. Paravets (160) were selected having equal representation from 4 different parts of India (Gujarat, Rajasthan, Telangana and Odisha). Questionnaire and telephonic survey method was used for data collection. The information needs on AI among paravets was assessed under four sub-groups, viz. information needs on heat detection, AI techniques, post-AI practices and miscellaneous information related to AI. Results revealed that the most needed information was symptoms of heat in buffaloes and pregnancy diagnosis both bearing 1st rank (MWS=7.22), followed by address of semen stations (MWS=6.09) with 2nd rank, rectal palpation (MWS=5.97) ranked 3rd, signs of heat in cattle (MWS=5.91) ranked 4th and use of crystoscope for checking fern pattern (MWS=5.68) with 5th rank. Earlier researchers have established that proper AI technique is essential for improving conception rate. Hence, along with provision of training courses on AI, there is a need to focus on creating efficient information systems platform incorporating knowledge related to AI which can be easily accessed by the paravets. Further, the paravets need to be trained on the use of digital tools for faster information delivery and ease of use. Efforts are also needed for continuous education of the paravets for enhancing the effectiveness of AI. Convergence of various information dissemination efforts of the government and non-government sector, and more number of multipronged information channels are required to fulfil the information needs of the paravets.

Determinants of use of gonadotropin formulations to treat female infertility in an Italian region

Abstract Background Gonadotropins (Gona) are commonly used for infertility treatment in women. They are available as urinary purified extracts (u-Gona) and recombinants (r-Gona). Objectives:We investigated if socio-demographic characteristics of treated women, year of dispensation and type of prescriber may play a role in the choice of a treatment with respect to the other. Methods From administrative healthcare databases, we collected claims of Gona for women, aged 18-45, enrolled in the health care system in the year preceding the first prescription (years 2009-2018). Treatment cycles were defined as a 21-day mobile time window. We identified three groups: u-Gona, r-Gona and treatment with both formulations (m-Gona). To identify determinants, we linked women with the socio-economic position (SEP), a composite deprivation index, based on the 2011 Italian Census data,categorized in 4 levels (high, medium-high, medium-low, low). We associated prescriptions with the type of prescriber, distinguishing between specialists and general practitioners. Results We retrieved 38,598 Gona treatment cycles prescribed to 23,412 women and distributed between formulations as follows: r-Gona 47.4%, u-Gona 26.3% and m-Gona 26.3%. The median age was 38 years for r-Gona and u-Gona, 39 for m-Gona. Comparing the type of prescriber, specialists were more likely to prescribe u-Gona instead of r-Gona (OR: 2.14; 95%CI: 2.02-2.27) than general practitioners. No relation was found between SEP and Gona formulations, except for patients with the highest level who were more likely to be prescribed with r-Gona instead of u-Gona (OR: 1.12; 95%CI: 1.03-1.21). Time trends showed that treatment choices changed over time. Conclusions In the Lazio region, the choice of gonadotropin formulations varied over time and was found to be related to the prescriber and partly to socio-economic position. An analysis of outcomes related to different treatments is under way. Key messages More and more women are using artificial insemination techniques; it is important to monitor fertility drug use patterns and determinants of the choice of a treatment. The use of fertility drugs is a multifactorial phenomenon and a matter of public health interest.

Biosimilar formulations of gonadotropins in a central Italian region: use patterns and time trends

Abstract Background Gonadotropins (Gonas) are hormones that play a central role in the follicular growth of women but also useful in male infertility. Gonas used for infertility treatment in women are available as urinary extracts or recombinants. In 2015, the first biosimilar of follitropin alfa was authorised. Objectives We aimed to evaluate use patterns, temporal and spatial variations of Gonas biosimilar formulations in an central Italian region. Methods From administrative healthcare databases of Lazio region, we extracted prescriptions that have occurred over the years 2015-2019. Gona's use was grouped as treatment with urinary (u) and recombinant (r). Special focus was made on biosimilar r-Gona. Use patterns were investigated with respect to characteristics of the patients treated, time trends, differences among local health units and type of prescribing physician. Results For the study period, we retrieved 136,333 Gona dispensations for 22,325 patients. The 10,927 r-Gona biosimilar dispensations (8.01% of all claims, of which 99.43% Bemfola®, and 0.57% Ovaleap®), were mostly referred to women, aged 18-45 years, (87.11% of all patients). Median age was 37 years for women, 38 for men. Since its authorisation, biosimilar r-Gona steadily gained market from 2015 (0,4% of claims) to 2017 (22,7%) followed by a decrease in 2019 (4,3%). Biosimilars claims are more frequent in treating men (10,8%) than women (6,1%). Most biosimilar prescriptions were issued by general practitioners (86.4%), followed by specialists (10.2%) and others. Biosimilar use patterns varied between different local health units (IQR 3.2%-8,5%). Conclusions In clinical pratice, r-Gona biosimilar dispensation, 8,8% of Gonas. are mostly prescribed from general practitioners and are proportionately more targeted at men than women. Further investigation is needed. Key messages More and more men and women are using artificial insemination techniques; it is important to monitor fertility drug use patterns. In times of savings for the economic system,the use of the biosimilar drugs is of increasing interest.

Effect of rectal temperature on efficiency of artificial insemination and embryo transfer technique in dairy cattle during hot season

This study aimed to evaluate the effects of rectal temperature (RT) as well as artificial insemination (AI) technique on pregnancy rates in Holstein cows and artificial insemination or embryo transfer (ET) techniques in Holstein heifers during summer. The experiments were conducted on five dairy farms in Hungary, where 1 631 data were examined. The RT was measured immediately before reproductive techniques using a digital thermometer. The effects of animal group and month on RT were analysed. The enhancement of RT until September can be seen in heifers, but the highest RT of cows occurred during July. In line with the enhancement of RT the pregnancy rate decreased in all groups; the lowest (cows) and the highest (heifers) value was observed in July. Moderate to high negative coefficients of correlation were detected between rectal temperature and pregnancy rate for particular groups (r = −0.3 – −0.7). More than 92% of ET, 48% and 16% of inseminated heifers and cows were pregnant up to the category of 39.1&lt;sup&gt; &lt;/sup&gt;°C of RT, above this it did not change considerably in the inseminated groups. In inseminated animals with RT higher than 39.1 °C significantly higher services per conception could be observed contrary to ET heifers. ET may become a more effective strategy to improve pregnancy success in heifers compared to AI during summer.

Dairy Cattle Cross-breeding in Ethiopia: Challenges and Opportunities: A Review

A dairy cattle crossbreeding has been started in 1930s to improve cow milk production, using exotic breeds such as Holstein Friesian and Jersey to exploit breed complementarities of milk production and reproductive traits. Crossbred cows with 50% to 75% blood level able to produce more milk whereas, reproductive performance has decreased as exotic blood level exceeds beyond 50%. Environment and genotype mismatch, lack of appropriate breeding policy and recording system, poor infrastructure, lack of trained man power, improper use of artificial insemination and low adoption of breeding technologies are some of the constraints of dairy cattle crossbreeding in Ethiopia. Defining the suitable breed and blood level for specific production system, continuous skill updating for artificial insemination technicians and introducing appropriate recording system are the most critical points that should be considered during proposing of cross-breeding program for dairy cattle. The purpose of this review was to look at the challenges and opportunities of dairy cattle crossbreeding programs in Ethiopia.

Artificial Insemination Applications and the Factors Affecting Its Success in Dairy Cattle Enterprises in Yahyalı District of Kayseri Province

Bu çalışmada Kayseri İli Yahyalı ilçesinde soy kütüğüne üye süt sığırcılık işletmelerinde yapılan suni tohumlama uygulamaları, bu uygulamaların başarısı, suni tohumlama uygulamaları sırasında karşılaşılan problemler ve bunlara ait çözüm önerilerinin getirilmesi amaçlandı. Bu çalışma, Kayseri ili Yahyalı ilçesi Damızlık Sığır Yetiştiricileri Birliğine üye 16 süt sığırı işletmesinde yürütüldü. Araştırma materyalini, yetiştiricilerden yüz yüze anket yoluyla elde edilen veriler oluşturmuştur. Çalışmada 16 süt sığırı işletmesi; ilkine buzağılama yaşı (hedef değer; ≤25 ay), buzağılama aralığı (hedef değer;