Suspended Animation Research Articles

Melihat Peluang Bisnis dan Usaha dengan Critical Thinking Ability oleh Mahasiswa IAIN Takengon

This research is motivated by the importance of critical thinking skills in seeing business and business opportunities, especially for students of sharia economics and sharia banking study programs/departments to support business opportunities and businesses that they are currently running and will be running. This research aims to analyze the ability to see business and business opportunities of Sharia economics and Sharia banking students using critical thinking skills. This research uses descriptive mixing method. Data was collected by randomly distributing Likert scale questionnaires and interview to 50 respondents from Sharia economics and sharia banking study program students. Percentage statistics are used to provide a clear picture of data distribution. Research findings show that the critical thinking possessed by respondents is generally in the sufficient category when looking at business and business opportunities. Respondents have sufficient systematic thinking skills, have sufficient business instincts to start a business, are able to consider various solutions quite well, consider implications and identify problems and potential solutions quite well, but due to limited energy, time and funds, sometimes business what they do is like suspended animation. This research is significant in providing an understanding of the ability of business opportunities by using the critical thinking skills that are requested and required and trained in formal education carried out by students.

Exhaustion of neurotransmitters storage

Introduction: Synaptic vesicles are located on presinaptic nerve terminals that accumulate high quantities of neurotransmitters, which are subsequently secreted by fusion with the presynaptic plasma membrane. Neuropeptides are stored in this dense core vesicles. Because of the vesicular storage of neuropeptides when a neuron is activated it rapidly release them in a relatively large bolus Material and methods: There were analyzed articles from PubMed and ScienceDirect database from the last 5 years, 2019-2024, mentioned such words as “neurotransmitters”, “synaptic vesicles”, “secretory granules” [1], and also up to 2019, representing the relevance of today [2-4]. Results: Neurotransmitters mediated neural communication. Secretory granules store release neuropeptides in response to various stimuli synaptic vesicles undergo. To support rapid and repeated rounds of release, synaptic vesicles undergo a trafficking cycle in the nerve terminal, on the cell membrane [5], and mitochondria due to Ca++ mpt pore [6], which has important clinical significance for the creation of artificial hibernation for the purpose of restoration and neuropeptides, neurotransmitters, neuromodulators. Including reducing the disorder of pain signal transmission in the chain of Transduction, Transmission, Modulation, Perception and many others [7-10]. Synaptic vesicle recycling is one of the best-studied cellular pathways. Many of the proteins involved are known, and their interections are becoming increasingly clear. Synaptic vesicles bind soluble cofactor proteins, with low affinity, and thus control theirs availability in the synapse, forming a buffer for cofactor proteins. Conclusion: For example in glutamatergic nerve endings, synaptic vesicle accumulate and store a proplrtion of the cellular glutamate pool and, in response to appropriate signals release glutamate into the synaptic cleft by exocytosis. Glutamate accumulation is accomplished by virtue of a glutamate uptake system [11] present in the synaptic vesicle membrane. The uptake system consists of a transport protein notably specific for glutamate which provides the coupling between ATP hydrolysis and glutamate transport. Having the goal of maintaining homeostasis as the basis of integral of functional systems taking into account clinical pathophysiology [12-19]. In mathematical and geometric interpretations [18] to improve Total Quality Management (TQM) in diagnostics based on markers [20,21] and of treatment in medicine and preserving human medical resources [22,23].

A mitochondrial redox switch licenses the onset of morphogenesis in animals.

Embryos undergo pre-gastrulation cleavage cycles to generate a critical cell mass before transitioning to morphogenesis. The molecular underpinnings of this transition have traditionally centered on zygotic chromatin remodeling and genome activation1,2, as their repression can prevent downstream processes of differentiation and organogenesis. Despite precedents that oxygen depletion can similarly suspend development in early embryos3-6, hinting at a pivotal role for oxygen metabolism in this transition, whether there is a bona fide chemical switch that licenses the onset of morphogenesis remains unknown. Here we discover that a mitochondrial oxidant acts as a metabolic switch to license the onset of animal morphogenesis. Concomitant with the instatement of mitochondrial membrane potential, we found a burst-like accumulation of mitochondrial superoxide (O2 -) during fly blastoderm formation. In vivo chemistry experiments revealed that an electron leak from site IIIQo at ETC Complex III is responsible for O2 - production. Importantly, depleting mitochondrial O2 - fully mimics anoxic conditions and, like anoxia, induces suspended animation prior to morphogenesis, but not after. Specifically, H2O2, and not ONOO-, NO, or HO•, can single-handedly account for this mtROS-based response. We demonstrate that depleting mitochondrial O2 - similarly prevents the onset of morphogenetic events in vertebrate embryos and ichthyosporea, close relatives of animals. We postulate that such redox-based metabolic licensing of morphogenesis is an ancient trait of holozoans that couples the availability of oxygen to development, conserved from early-diverging animal relatives to vertebrates.

Biogenesis and Regulation of the Freeze–Thaw Responsive microRNA Fingerprint in Hepatic Tissue of Rana sylvatica

Background: Freeze-tolerant animals undergo significant physiological and biochemical changes to overcome challenges associated with prolonged whole-body freezing. In wood frog Rana sylvatica (now Lithobates sylvaticus), up to 65% of total body water freezes in extracellular ice masses and, during this state of suspended animation, it is completely immobile and displays no detectable brain, heart, or respirometry activity. To survive such extensive freezing, frogs integrate various regulatory mechanisms to ensure quick and smooth transitions into or out of this hypometabolic state. One such rapid and reversible regulatory molecule capable of coordinating many aspects of biological functions is microRNA. Herein, we present a large-scale analysis of the biogenesis and regulation of microRNAs in wood frog liver over the course of a freeze–thaw cycle (control, 24 h frozen, and 8 h thawed). Methods/Results: Immunoblotting of key microRNA biogenesis factors showed an upregulation and enhancement of microRNA processing capacity during freezing and thawing. This was followed with RT-qPCR analysis of 109 microRNA species, of which 20 were significantly differentially expressed during freezing and thawing, with the majority being upregulated. Downstream bioinformatics analysis of miRNA/mRNA targeting coupled with in silico protein–protein interactions and functional clustering of biological processes suggested that these microRNAs were suppressing pro-growth functions, including DNA replication, mRNA processing and splicing, protein translation and turnover, and carbohydrate metabolism. Conclusions: Our findings suggest that this enhanced miRNA maturation capacity might be one key factor in the vital hepatic miRNA-mediated suppression of energy-expensive processes needed for long-term survival in a frozen state.

A Critique of a Satire - Constantine Acropolites’ Letter on the <i>Timarion</i>

The protagonist of the satire Timarion, by an unknown author from the 11th or 12th century, falls into a state of suspended animation on his journey and is carried by two demons into the underworld, where he proves in a court trial that he is not dead and is allowed to return to the living. The aim of this paper is to present and interpret a short letter written two hundred years later by Constantine Acropolites, who sharply criticises the Timarion, without clearly presenting his objections.

Sub-zero non-freezing of vascularized composite allografts in a rodent partial hindlimb model

Ischemia is a major limiting factor in Vascularized Composite Allotransplantation (VCA) as irreversible muscular injury can occur after as early as 4–6 h of static cold storage (SCS). Organ preservation technologies have led to the development of storage protocols extending rat liver ex vivo preservation up to 4 days. Development of such a protocol for VCAs has the added challenge of inherent ice nucleating factors of the graft, therefore, this study focused on developing a robust protocol for VCA supercooling. Rodent partial hindlimbs underwent subnormothermic machine perfusion (SNMP) with several loading solutions, followed by a storage solution with cryoprotective agents (CPA) developed for VCAs. Storage occurred in suspended animation for 24h and VCAs were recovered using SNMP with modified Steen. This study shows a robust VCA supercooling preservation protocol in a rodent model. Further optimization is expected to allow for its application in a transplantation model, which would be a breakthrough in the field of VCA preservation.

Analyzing embryo dormancy at single-cell resolution reveals dynamic transcriptional responses and activation of integrin-Yap/Taz prosurvival signaling

Embryonic diapause is a reproductive adaptation that enables some mammalian species to halt the otherwise continuous pace of embryonic development. In this dormant state, the embryo exploits poorly understood regulatory mechanisms to preserve its developmental potential for prolonged periods of time. Here, using mouse embryos and single-cell RNA sequencing, we molecularly defined embryonic diapause at single-cell resolution, revealing transcriptional dynamics while the embryo seemingly resides in a state of suspended animation. Additionally, we found that the dormant pluripotent cells rely on integrin receptors to sense their microenvironment and preserve their viability via Yap/Taz-mediated prosurvival signaling.

Evolution of diapause in the African turquoise killifish by remodeling the ancient gene regulatory landscape

Suspended animation states allow organisms to survive extreme environments. The African turquoise killifish has evolved diapause as a form of suspended development to survive a complete drought. However, the mechanisms underlying the evolution of extreme survival states are unknown. To understand diapause evolution, we performed integrative multi-omics (gene expression, chromatin accessibility, and lipidomics) in the embryos of multiple killifish species. We find that diapause evolved by a recent remodeling of regulatory elements at very ancient gene duplicates (paralogs) present in all vertebrates. CRISPR-Cas9-based perturbations identify the transcription factors REST/NRSF and FOXOs as critical for the diapause gene expression program, including genes involved in lipid metabolism. Indeed, diapause shows a distinct lipid profile, with an increase in triglycerides with very-long-chain fatty acids. Our work suggests a mechanism for the evolution of complex adaptations and offers strategies to promote long-term survival by activating suspended animation programs in other species.

Dynamic Modulation of Li2O2 Growth in Li‐O2 Batteries through Regulating Oxygen Reduction Kinetics with Photo‐Assisted Cathodes

AbstractWidely acknowledged that the capacity of Li‐O2 batteries (LOBs) should be strongly determined by growth behaviors of the discharge product of lithium peroxide (Li2O2) that follows both coexisting surface and solution pathways. However until now, it remains still challenging to achieve dynamic modulation on Li2O2 morphologies. Herein, the photo‐responsive Au nanoparticles (NPs) supported on reduced oxide graphene (Au/rGO) have been utilized as cathode to manipulate oxygen reduction reaction (ORR) kinetics by aid of surface plasmon resonance (SPR) effects. Thus, we can experimentally reveal the importance of matching ORR kinetics with Li+ migration towards battery performance. Moreover, it is found that Li+ concentration polarization caused “sudden death” of LOBs is supposed to be just a form of suspended animation that could timely recover under irradiation. This work provides us an in‐depth explanation on the working mechanism of LOBs from a kinetic perspective, offering valuable insights for the future battery design.

Dynamic Modulation of Li2O2 Growth in Li-O2 Batteries through Regulating Oxygen Reduction Kinetics with Photo-Assisted Cathodes.

Widely acknowledged that the capacity of Li-O2 batteries (LOBs) should be strongly determined by growth behaviors of the discharge product of lithium peroxide (Li2O2) that follows both coexisting surface and solution pathways. However until now, it remains still challenging to achieve dynamic modulation on Li2O2 morphologies. Herein, the photo-responsive Au nanoparticles (NPs) supported on reduced oxide graphene (Au/rGO) have been utilized as cathode to manipulate oxygen reduction reaction (ORR) kinetics by aid of surface plasmon resonance (SPR) effects. Thus, we can experimentally reveal the importance of matching ORR kinetics with Li+ migration towards battery performance. Moreover, it is found that Li+ concentration polarization caused "sudden death" of LOBs is supposed to be just a form of suspended animation that could timely recover under irradiation. This work provides us an in-depth explanation on the working mechanism of LOBs from a kinetic perspective, offering valuable insights for the future battery design.

The future of artificial hibernation medicine: protection of nerves and organs after spinal cord injury.

Spinal cord injury is a serious disease of the central nervous system involving irreversible nerve injury and various organ system injuries. At present, no effective clinical treatment exists. As one of the artificial hibernation techniques, mild hypothermia has preliminarily confirmed its clinical effect on spinal cord injury. However, its technical defects and barriers, along with serious clinical side effects, restrict its clinical application for spinal cord injury. Artificial hibernation is a future-oriented disruptive technology for human life support. It involves endogenous hibernation inducers and hibernation-related central neuromodulation that activate particular neurons, reduce the central constant temperature setting point, disrupt the normal constant body temperature, make the body "adapt" to the external cold environment, and reduce the physiological resistance to cold stimulation. Thus, studying the artificial hibernation mechanism may help develop new treatment strategies more suitable for clinical use than the cooling method of mild hypothermia technology. This review introduces artificial hibernation technologies, including mild hypothermia technology, hibernation inducers, and hibernation-related central neuromodulation technology. It summarizes the relevant research on hypothermia and hibernation for organ and nerve protection. These studies show that artificial hibernation technologies have therapeutic significance on nerve injury after spinal cord injury through inflammatory inhibition, immunosuppression, oxidative defense, and possible central protection. It also promotes the repair and protection of respiratory and digestive, cardiovascular, locomotor, urinary, and endocrine systems. This review provides new insights for the clinical treatment of nerve and multiple organ protection after spinal cord injury thanks to artificial hibernation. At present, artificial hibernation technology is not mature, and research faces various challenges. Nevertheless, the effort is worthwhile for the future development of medicine.

Biopreservation Beyond the Biosphere: Exploring the Ethical, Legal & Social Implications of Suspended Animation in Space.

In the evolving field of advanced biopreservation technologies, the development of suspended animation (SA) is inspired by real-world challenges. In the context of space exploration, SA is seen as a solution to enable humans to undertake missions far beyond low Earth orbit, including routine travel to other planets in our solar system and beyond. While work on the socio-ethical and legal implications (ELSI) of space exploration continues to evolve, NASA has committed to make ethics a priority issue, making this a fruitful field for further examination.

Condition of the problem of organ transplantation in the USSR in 1970–1971 and ways to solve it (Yu.M. Lopukhin, A.G. Lapchinsky, V.P. Demikhov)

Aim. The article examines and analyzes the state of the problem of organ transplantation in the USSR in the early 1970s and ways to solve it with the participation of three prominent transplantologists of the Soviet Union – Yu.M. Lopukhin, A.G. Lapchinsky and V.P. Demikhov, as well as some world achievements in this field.Material and methods. Available printed works on the history of Russian and world transplantology in the 1970s, the analysis of which was carried out by historical-genetic and comparative-analytical methods.Results. Developed by Yu.M. Lopukhin together with leading surgeons and immunologists of the country the Program of scientific research on the problem of transplantation of organs and tissues included three main areas: clinical problems of transplantation of organs and body parts (limbs), including ensuring the viability of organs and tissues outside the body, the ways to overcome biological incompatibility, and also the moral and ethical issues of transplantation, and donation issues. It is shown that the methods of overcoming biological incompatibility in organ and tissue allotransplantations, which had been used for many years by A.G. Lapchinsky and V.P. Demikhov (exchange transfusion of blood between organisms of different species, cross circulation between individuals of the same species), no longer corresponded to the state of medical science, which was represented by Yu.M. Lopukhin. However, scientists who had world-class priorities for the duration of survival of experimental animals with a transplanted limb (A.G. Lapchinsky) and an additional heart and lung transplanted into the chest (V.P. Demikhov) could help in the implementation of some provisions of the Lopukhin’s Program. The preservation of organs and tissues before transplantation in a state of suspended animation was studied by A.G. Lapchinsky, and V.P. Demikhov worked at developing the methods for the extracorporeal connection of organs to the body of an intermediate host and using portable artificial hearts to maintain the vital activity of a cadaveric heart before transplantation.Conclusions. Pioneers of experimental transplantology in the USSR A.G. Lapchinsky and V.P. Demikhov, who began their research in the 1940s, still could have solved many issues of transplanting organs and body parts by the 1970s. But in presented in 1971 Program on the problem of organ and tissue transplantation, prepared under the guidance of Yu.M. Lopukhin, the emphasis was placed on immunological studies, while experimental surgeons played a minor role in it.

Application of artificial hibernation technology in acute brain injury.

Controlling intracranial pressure, nerve cell regeneration, and microenvironment regulation are the key issues in reducing mortality and disability in acute brain injury. There is currently a lack of effective treatment methods. Hibernation has the characteristics of low temperature, low metabolism, and hibernation rhythm, as well as protective effects on the nervous, cardiovascular, and motor systems. Artificial hibernation technology is a new technology that can effectively treat acute brain injury by altering the body's metabolism, lowering the body's core temperature, and allowing the body to enter a state similar to hibernation. This review introduces artificial hibernation technology, including mild hypothermia treatment technology, central nervous system regulation technology, and artificial hibernation-inducer technology. Upon summarizing the relevant research on artificial hibernation technology in acute brain injury, the research results show that artificial hibernation technology has neuroprotective, anti-inflammatory, and oxidative stress-resistance effects, indicating that it has therapeutic significance in acute brain injury. Furthermore, artificial hibernation technology can alleviate the damage of ischemic stroke, traumatic brain injury, cerebral hemorrhage, cerebral infarction, and other diseases, providing new strategies for treating acute brain injury. However, artificial hibernation technology is currently in its infancy and has some complications, such as electrolyte imbalance and coagulation disorders, which limit its use. Further research is needed for its clinical application.

Optimalisasi Wisata Kampung Batik Semarang Sebagai Permukiman Yang Berbasis Sejarah

Having been a center for batik activities in the past but experiencing suspended animation in 1970, the condition of the batik village after independence actually experienced a dark period including the title of being a slum village, unsafe because it was a den of criminals and always flooded. Kampung batik is known as an ancient village, whose formation coincided with the history of the city of semarang, its fate experienced ups and downs. However, in 1980, batik craftsmen attempted to revive semarang batik production, although it was not yet optimal due to capital constraints and inadequate human resources in terms of skills. Starting from the concerns that occurred in this historic settlement, the semarang city government carried out the revitalization of the batik village in 2005 and experienced rapid progress until the enactment of regional regulation (perda) no. 14 of 2011 concerning the establishment of batik villages as cultural heritage through the establishment of a thematic village program. The research method is qualitative research with historical methods, which includes heuristics (collecting sources), criticism, interpretation, and writing down facts. The data collection techniques used were interviews, observation and document study. The results of the research can be concluded that batik villages with cultural potential and through community empowerment have become one of the batik producing areas as well as cities with cultural tourism destinations in central java province.

Symbolism of Strategy for Affirmative Disobedience in Cultural Entrepreneurship: The Case of the Ethnographic Museum of Kendebay Karabdolov, Shymkent, Kazakhstan

Research on the influence of traditions and religion on cultural entrepreneurship usually focuses on how religion and tradition support entrepreneurial activity. The questions of how traditional ideas and religion can support cultural entrepreneurship remain outside the scope of much research. To understand this phenomenon, where through entrepreneurship the symbols and structures of the national tradition, as well as the religious aspects of the ethnic group, are recreated, an ethnographic study was carried out. In the context of the study, an important place is occupied by the fact that we are considering a period equally divided into a 30-year hibernation of culture (during Soviet rule) and its dynamic revival with Kazakhstan’s independence. In the process of inductive research, a dynamic model of the process was identified, highlighting the factors of accumulation of material within the culture and starting the process of its working and retransmission. This model shows how cultural hibernation served as a trigger for the launch of the process of restoring culture and supporting the national heritage through entrepreneurship. During the period of special intolerance of the Soviet authorities, entering into suspended animation was able to preserve traditions in a state of non-manifestation, creating a shell for the accumulation of all centuries of experience. Gradually, this shell became permeable, and the process of cultural dynamics began. There the skills built in by the environment were transformed into actions and demonstrated through active practices. Thus, the relaying of cultural material could become the basis for supporting religion and tradition through cultural entrepreneurship. The study proposes to consider entrepreneurship as a means of preserving the intangible and material (through objects and processes) cultural heritage, which carries both archaic symbols and constructs and is clothed in a new layer of modern ethnic symbols.

LITERASI KEUANGAN DIGITAL SEBAGAI UPAYA PENDUKUNG PEREKONOMIAN

Kurungan Nyawa Village in Pesawaran District shows signs of weakness in BUMDes management. The implementation of BUMDes in Kurungan Nyawa Village is constrained by three main problems, namely economic, social, and human potential issues. Academics who can help improve the management and administration of BUMDes business units that are not producing at peak production levels, even in a state of suspended animation, can help solve three problems faced by BUMDes in Kurungan Nyawa Village, Pesawaran Regency.

A novel nematode species from the Siberian permafrost shares adaptive mechanisms for cryptobiotic survival with C. elegans dauer larva.

Some organisms in nature have developed the ability to enter a state of suspended metabolism called cryptobiosis when environmental conditions are unfavorable. This state-transition requires execution of a combination of genetic and biochemical pathways that enable the organism to survive for prolonged periods. Recently, nematode individuals have been reanimated from Siberian permafrost after remaining in cryptobiosis. Preliminary analysis indicates that these nematodes belong to the genera Panagrolaimus and Plectus. Here, we present precise radiocarbon dating indicating that the Panagrolaimus individuals have remained in cryptobiosis since the late Pleistocene (~46,000 years). Phylogenetic inference based on our genome assembly and a detailed morphological analysis demonstrate that they belong to an undescribed species, which we named Panagrolaimus kolymaensis. Comparative genome analysis revealed that the molecular toolkit for cryptobiosis in P. kolymaensis and in C. elegans is partly orthologous. We show that biochemical mechanisms employed by these two species to survive desiccation and freezing under laboratory conditions are similar. Our experimental evidence also reveals that C. elegans dauer larvae can remain viable for longer periods in suspended animation than previously reported. Altogether, our findings demonstrate that nematodes evolved mechanisms potentially allowing them to suspend life over geological time scales.

The intestinal microbiota and metabolic profiles of Strauchbufo raddei underwent adaptive changes during hibernation.

The intestinal microbiota help regulate hibernation in vertebrates. However, it needs to be established how hibernation modulates the gut microbiome and intestinal metabolism. In the present study, we used an artificial hibernation model to examine the responses of the gut microbiota of the Strauchbufo raddei to the environmental changes associated with this behavior. Hibernation significantly lowered the diversity of the microbiota and altered the microbial community of the gut. Proteobacteria, Firmicutes, and Bacteroidota were the major bacterial phyla in the intestines of S. raddei. However, Firmicutes and Proteobacteria predominated in the gut of active and hibernating S. raddei, respectively. Certain bacterial genera such as Pseudomonas, Vibrio, Ralstonia, and Rhodococcus could serve as biomarkers distinguishing hibernating and non-hibernating S. raddei. The gut microbiota was more resistant to environmental stress in hibernating than active S. raddei. Moreover, metabolomics revealed that metabolites implicated in fatty acid biosynthesis were highly upregulated in the intestines of hibernating S. raddei. The metabolites that were enriched during hibernation enabled S. raddei to adapt to the low temperatures and the lack of exogenous food that are characteristic of hibernation. A correlation analysis of the intestinal microbiota and their metabolites revealed that the gut microbiota might participate in the metabolic regulation of hibernating S. raddei. The present study clarified the modifications that occur in the intestinal bacteria and their symbiotic relationship with their host during hibernation. These findings are indicative of the adaptive changes in the metabolism of amphibians under different environmental conditions.

Hibernating bat species of Belarus: results of the work of the Minsk bat contact centre ‘Kazhanapolis’ (2018–2022)

Bat rehabilitation centres, in addition to their main goal, namely, animal rescue, may provide a significant scientific information, and are also sites for popular science activities. This article presents the results of the work of the Minsk Bat contact centre ‘Kazhanapolis’ in 2018–2022. The work of the Сentre includes the rescue of animals by means of artificial hibernation with subsequent release into the wild. Species identification is carried out directly when possible, but sometimes also by photographs. During the hibernation seasons (October–March) in 2018 to 2022, 565 finds were recorded, accounting for more than 730 individuals of 10 species of bats, including Barbastella barbastellus, Epte-sicus nilssonii, E. serotinus, Myotis dasycneme, M. daubentonii, Nyctalus noctu-la, Pipistrellus kuhlii, P. pygmaeus, Plecotus auritus, and Vespertilio murinus. Data were collected from 56 unique localities. Compared to the previous period of work (2007–2017), an increase in the number of recorded species, in the total number of individuals, as well as in the number of localities is shown. By the number of finds (55.47%), as well as recorded specimens (71.32%), V. murinus prevails. A significant proportion (more than 5%) of records are those of N. noctula (23.28% ind. and 8.31% finds), E. serotinus (8.9% ind. and 11.32% finds), and E. nilssonii (5.75% ind. and 3% finds). We consider the record of P. pygmaeus as accidental or migrant. Most of the finds of V. murinus, E. serotinus, P. auritus, P. kuhlii, M. dasycneme, and P. pygmaeus were represented by solitary individuals. N. noctula, E. nilssonii, M. daubentonii, and B. barbastellus are mainly recorded in colonies. The largest colony size (more than 100 ind.) was recorded for N. noctula. The sex ratio (M : F) ranges from 33.3 : 66.6% in E. nilssonii to 74.2 : 25.8% in N. noctula. Plecotus auritus and Myotis dasycneme are represented exclusively by females. Thus, we state the dominance in the species structure of wintering ‘urban bat species’ in Belarus of V. murinus and, at the same time, the expansion of the winter range of N. noctula and P. kuhlii. In addition, the collected data signifi-cantly contribute to our knowledge on the winter range and some hibernation traits of B. barbastellus, M. dasycneme, E. nilssonii, and E. serotinus in Belarus and Eastern Europe.