Two distinct phases of bed-level adjustment over the last 150 years are identified for the principal alluvial reaches of the Arno River (Upper Valdarno and Lower Valdarno). The planimetric configuration of the river in these reaches is the result of a series of hydraulic works (canalization, rectification, artificial cut-offs, etc.) carried out particularly between the 18th and the 19th centuries. Subsequently, a series of interventions at basin level (construction of weirs, variations in land use), intense instream gravel-mining after World War II, and the construction of two dams on the Arno River, caused widespread degradation of the streambed. Since about 1900, total lowering of the channel bed is typically between 2 and 4 m in the Upper Valdarno Reach and between 5 and 8 m in some areas of the Lower Valdarno Reach. Bed-level adjustments with time are analyzed for a large number of cross-sections and described by an exponential-decay function. This analysis identified the existence of two main phases of lowering: the first, triggered at the end of the past century; the second, triggered in the interval 1945–1960 and characterized by more intense degradation of the streambed. The first phase derived from changes in land-use and land-management practices. The second phase is the result of the superimposition of two factors: intense instream mining of gravel, and the construction of the Levane and La Penna dams.