Artemisia Princeps Research Articles

Cookie Quality Characteristics Influenced by the Proportion of Wheat Flour Replaced by <i>Artemisia princeps</i> Leaf Powder

Cookie Quality Characteristics Influenced by the Proportion of Wheat Flour Replaced by <i>Artemisia princeps</i> Leaf Powder

A Review on Artemisia princeps: Pharmacology and Anti-Inflammatory Potential of Phytoconstituents

Higher organisms developed the inflammation as a defence mechanism against infection and injuries. Chronic Inflammation is a progressive reaction which end at damage of living tissues. Several synthetic compounds had been used for fighting against uncontrolled inflammation. However, some of these compounds had significant adverse along with their anti-inflammatory properties. So, there is still need of some new anti-inflammatory compounds with least or no adverse effects. Numerous herbs including Ashwagandha, Calendula, Cat’s claw; Chamomile, Cilantro, Licorice root, Parsley, Curcuma longa, Zingiber officinale, Rosmarinus officinale, Borago officinale, Rosemary, Evening primrose, Devil’s claw had been evaluated for their anti-inflammation potentials and potent results was obtained with non-significant adverse effects. Plant named as Artemisia princeps. Family-Asteraceae has been evaluated for its pharmacological abilities and proved as significant herb for treatment of various pathological conditions. This review comprises the pharmacological abilities of Artemisia princeps. Linn and its Phytoconstituents with special emphasis on its anti-inflammatory potential. In the current literature survey, Selected plant was found to contain substantial number of phytoconstituent with pharmacological abilities Including Apigenin, Diosmetin, Eriodicytol, Eupafolin, Kaempferol, Vitex, Jaceosidine and Querecetine. Further literature survey also provides the anti-inflammatory potentials of phytoconstituents present in Artemisia princeps.

Formulation and Evaluation of Emulgel loaded with leaf extract of Artemisia princeps for its Anti-Inflammatory potential

Indian Systems of Medicine have their roots in ayurveda. The potential of naturally occurring bioactive components is of tremendous interest to researchers. Different plant extracts have remedial properties.North India, Japan, China, Korea and Nepal are among the countries where Artemisia princeps can be found.Apigenin, diosmetin, eriiodicytol, eupafolin, kaempferol, vitex, jaceosidine, and quercetins have all been discovered in plant extracts of Artemisia princeps. It is used to treat fever, apoptosis, pain, ulcers, inflammation, and other conditions as well as Diarrhoea and Asthma.Recent in-vivo and in-vitro research have shown that it has anti-inflammatory and analgesic properties. The objectives of proposed research work are to formulate, evaluate and optimize the Emulgel loaded with optimised extract of Artemisia princepsand assess its In-Vitro Anti-Inflammatory Activity. Collected leaves of Artemisia princeps was extracted using hydro-alcoholic solvent. On the basis of their Phytochemical evaluation, the extract was optimized. Additional 6 batches of Emulgel were created utilizing a chosen extract. The prepared Emulgel was assessed for pH, spreadability, viscosity, consistency, appearance, colour, infrared spectral assignment, and appearance. Using the HRBC Membrane stabilisation assay, extract and formulation were tested for in vitro anti-inflammatory efficacy. The suggested study project's findings support the potential for future topical application of the newly developed Emulgel, which contains Artemisia princeps leaf extract, to reduce inflammation.

Halotolerant endophytic bacteria alleviate salinity stress in rice (oryza sativa L.) by modulating ion content, endogenous hormones, the antioxidant system and gene expression

Excessive salinity reduces crop production and negatively impacts agriculture worldwide. We previously isolated endophytic bacterial strains from two halophytic species: Artemisia princeps and Chenopodium ficifolium. We used three bacterial isolates: ART-1 (Lysinibacillus fusiformis), ART-10 (Lysinibacillus sphaericus), and CAL-8 (Brevibacterium pityocampae) to alleviate the impact of salinity stress on rice. The impact of 160 mM NaCl salinity on rice was significantly mitigated following inoculation with these bacterial strains, resulting in increased growth and chlorophyll content. Furthermore, OsNHX1, OsAPX1, OsPIN1 and OsCATA expression was increased, but OsSOS expression was decreased. Inductively coupled plasma mass spectrometry (ICP-MS) revealed reduced K+ and Na+ levels in shoots of bacteria-inoculated plants, whereas that of Mg2+ was increased. Bacterial inoculation reduced the content of total flavonoids in rice leaves. Salinized plants inoculated with bacteria showed reduced levels of endogenous salicylic acid (SA) and abscisic acid (ABA) but increased levels of jasmonic acid (JA). In conclusion, the bacterial isolates ART-1, ART-10, and CAL-8 alleviated the adverse effect of salinity on rice growth, which justifies their use as an eco-friendly agricultural practice.

A Study on Awareness and Use of Edible Wild Vegetables in Special Forest Products

Purpose: Most processed wild vegetable products are dried, aged, and pickled vegetables. Developing processed wild vegetable foods is necessary to create new high-added value. Therefore, in this study, researchers tried to prepare basic data that can be utilized in various food manufacturing fields in the future through a survey of the awareness and use of edible wild vegetables that require the development of processed wild plants.
 Method: This paper investigated the awareness and use of wild vegetables, targeting adult men and women aged 20 or older residing in Daegu and Gyeongbuk. The survey consisted of questions about the perception, the degree of recognition, and the actual use of wild vegetables. As for the wild vegetable items of the awareness survey, 20 kinds of wild vegetables were selected with high awareness and mainly growing naturally in the Gyeongbuk area. Wild vegetable crops' perception and recognition degree were measured using a 5-point Likert scale. Data analysis was performed using the SPSS 27.0 program.
 Results: Regarding the degree of recognition of wild vegetables, 'Wild vegetables have a unique scent and taste' was the highest at 4.25. As a result of the knowledge survey for wild vegetable crops, 'Artemisia princeps,' 'Capsella bursa-pastoris,' 'Aralia elata', 'Allium monanthum,' 'Aster scaber', and 'Sedum sarmentosum' showed high awareness in the order. For purchase consideration, the 20s respondents of 61.3% considered 'freshness' the most. For purchase purposes, 'for salads' was the highest at 15.1% in the 30s, and 'for bibimbap' was 42.6% in the 60s and older. Most respondents did not use wild vegetables for 'green juice' and 'decoration.' 'For side dishes' was 70.4% in their 40s, the highest among all age groups(p<0.05).
 Conclusion: By investigating consumers' use and awareness of wild vegetables, the survey data can be used for distribution and marketing plans for edible wild vegetables, which are special forest products. In addition, the research results on the purchase purpose of wild vegetables, the actual use of wild vegetable products, and the degree of preference provide basic data for developing high-added-value products by using them in various processed foods and menu development.

Hydrogen Peroxide Measurement Can Be Used to Monitor Plant Oxidative Stress Rapidly Using Modified Ferrous Oxidation Xylenol Orange and Titanium Sulfate Assay Correlation

Various methodologies, sensitivities, and types of interference affect the quantification of plant hydrogen peroxide (H2O2) concentration. Modified ferrous oxidation xylenol orange (eFOX) assay and titanium sulfate (Ti(SO4)2 assay are relatively accessible methods. However, their correlation is unknown, for example whether we can get the same results for different species in different environments. Leaf samples of Ambrosia trifida, Solidago altissima, Artemisia princeps, and Sicyos angulatus were collected from a riparian vegetation zone on sunny days. The H2O2 concentration in the plant leaves was evaluated in two groups. Nonfrozen leaf samples were prepared for analysis soon after arriving at the laboratory, and frozen leaf samples were stored at −80 °C for 25 days and prepared afterwards. The eFOX assay can measure even lower fluctuations in H2O2 concentration than the Ti(SO4)2 assay. A substantial correlation was observed between nonfrozen and frozen samples in the eFOX (r = 0.879, p < 0.001) and Ti(SO4)2 assays (r = 0.837, p < 0.001). Sample weight did not affect H2O2 quantification. Each species showed a substantial correlation between the eFOX and Ti(SO4)2 assays in nonfrozen conditions (Ambrosia trifida (r = 0.767, p < 0.001), Solidago altissima (r = 0.583, p < 0.001), Artemisia princeps (r = 0.672, p < 0.001), and Sicyos angulatus (r = 0.828, p < 0.001)). Therefore, both methods can be utilized easily and rapidly to quantify oxidative stress using H2O2.

Eupatilin Alleviates Hyperlipidemia in Mice by Inhibiting HMG-CoA Reductase.

Artemisia princeps (family Asteraceae) is a natural product broadly used as an antioxidative, hepatoprotective, antibacterial, and anti-inflammatory agent in East Asia. In the present study, eupatilin, the main constituent of Artemisia princeps, was investigated as an antihyperlipidemic agent. Eupatilin inhibited 3-hydroxy-3-methylglutaryl (HMG)-CoA reductase (HCR), an enzyme that is a therapeutic target for hyperlipidemia, in an ex vivo assay using rat liver. In addition, oral administration of eupatilin significantly lowered the serum levels of total cholesterol (TC) and triglycerides (TG) in corn oil-induced and Triton WR-1339-induced hyperlipidemic mice. These results suggest that eupatilin can alleviate hyperlipidemia by inhibiting HCR.

A useful species identification and quality control using volatile patterns of ssajuari-ssuk and sajabal-ssuk (Artemisia princeps Pamp. cv. ssajuari and Artemisia princeps Pamp. cv. sajabal; Korean mugwort) according to air-drying term by fast gas chromatography with uncoated surface acoustic wave sensor.

Ssajuari-ssuk and sajabal-ssuk have many clinical benefits. It is difficult to discriminate between these two species based on general characteristics aside from the shapes of the leaves. Thus, species identification and quality control between ssajuari-ssuk and sajabal-ssuk are of great concern in plant science and clinical therapy. The aim of this study is to determine whether fast gas chromatography with uncoated surface acoustic wave sensor (GC-SAW) can be a useful technique for performing species identification and quality control using volatile patterns of ssajuari-ssuk and sajabal-ssuk air-dried for 4months and 2years and 4months. Fast GC-SAW sensor provides second unit analysis, simple, on-line measurements that do not require pretreatment of the sample and rapid sensory information. Headspace solid-phase microextraction gas chromatography-mass spectrometry (HS-SPME-GC-MS) was employed to confirm the identification of the volatiles and compared to fast GC-SAW sensor. In air-dried sajabal-ssuk, the concentration of 1,8-cineole was higher than that in air-dried ssajuari-ssuk, while the level of α-thujone was considerably lower than that of air-dried ssajuari-ssuk. Each of ssajuari-ssuk and sajabal-ssuk air-dried for 4months and 2years and 4months has its own characteristic volatile pattern owing to its individual chemotypes or chemical compositions. Consequently, the fast GC-SAW sensor can be a useful technique for species identification and quality control using volatile patterns of ssajuari-ssuk and sajabal-ssuk air-dried for 4months and 2years and 4months. This method can be used for the standardisation of quality control using volatile patterns of herbal medicines.

Role of Phenolic Acid Metabolism in Enhancing Bioactivity of Mentha Extract Fermented with Plant-Derived Lactobacillus plantarum SN13T

Plant-derived lactic acid bacteria are major fermentation organisms that can grow in medicinal herb extracts enriched with phytochemicals like glycosides, phenolic acids, flavonoids, and tannins. Fermentation with strain-specific Lactobacilli harboring metabolic enzymes can increase the bioactivity and bioavailability of medicinal herbs. Fermentation of extracts of Artemisia princeps and Paeonia lactiflora has been previously found to increase their bioactivities. Therefore, this study explores the possibility of increasing the bioactivity of Mentha arvensis (Mentha) extract against lipopolysaccharide (LPS)-induced RAW 264.7 macrophage cells by fermenting with plant-derived probiotic strains Lactobacillus (Lact.) plantarum SN13T and Pediococcus (Ped.) pentosaceus LP28. As a result, fermentation with SN13T significantly increased the bioactivity of Mentha extract as compared to unfermented or LP28-fermented extracts. This higher bioactivity was associated with the metabolism of rosmarinic acid (RA) and caffeic acid (CA), the major bioactive phenolic acids reported in Mentha, along with the production of the metabolite dihydrocaffeic acid (DHCA). DHCA was found to be a more potent LPS-induced nitric oxide (NO) inhibitor than its precursor phenolic acids. The metabolism of RA to DHCA via CA could be mediated by the enzymes cinnamoyl ester hydrolase and hydroxycinnamate reductases, encoded by the ceh gene and the hcrRABC gene operon, respectively, which were identified in the complete genome sequence of Lact. plantarum SN13T but were absent in Ped. pentosaceus LP28. The genes hcrA, hcrB, and hcrC were significantly and time-dependently overexpressed in Lact. plantarum SN13T when grown in the Mentha extract, suggesting the role of phenolic acid metabolism in enhancing its bioactivity.

Artemiprinolides A−M, thirteen undescribed sesquiterpenoid dimers from Artemisia princeps and their antihepatoma activity

Bioassay-guided investigation of the active fraction of Artemisia princeps led to 13 undescribed sesquiterpenoid dimers, artemiprinolides A−M (1–13), together with 11 known ones (14−24). Their structures were elucidated by comprehensive spectroscopic data and absolute configurations were assigned based on single crystal X-ray diffraction data and ECD calculations. Structurally, all compounds were postulated to be derived from the Diels–Alder cycloaddition. The isolated dimers except 11 and 15 were assayed for their cytotoxicity against HepG2, Huh7, and SK-Hep-1 cell lines, of which four compounds (3, 13, 17, 18) exhibited obvious cytotoxicity with IC50 values ranging from 8.8 to 20.1 μM. Interestingly, the most active compounds 1 and 16 manifested significant cytotoxicity on the three tested hepatoma cell lines with IC50 values of 5.4, 4.1 (HepG2), 7.7, 5.6 (Huh7), and 11.8, 15.7 μM (SK-Hep-1), respectively, which were better than sorafenib. Compound 1 dose-dependently inhibited cell migration and invasion, and significantly induced the HepG2 cell arrest in G2/M phase by downregulating cdc2 and pcdc2 and upregulating cyclinB1; and induced apoptosis by downregulating Bcl-2 expression and upregulating Bax level. The molecular docking study implied that the carbonyl at the C-12′ of 1 had a strong binding affinity with PRKACA.

Investigation of Asymptomatic Infection of Phellinus noxius in Herbaceous Plants.

The white-rot fungus Phellinus noxius is known to cause brown root rot disease (BRRD) in woody trees and shrubs. To understand the pathogenicity of P. noxius in herbaceous plants, we investigated 23 herbaceous weed and turfgrass species in 32 BRRD-infested sites in Taiwan and/or tested them by artificial inoculation. In the field survey, P. noxius was isolated from seven symptomless herbaceous species (i.e., Typhonium blumei, Paspalum conjugatum, Paspalum distichum, Oplismenus compositus, Bidens pilosa, Digitaria ciliaris, and Zoysia matrella). Potted plant inoculation assays suggested that P. noxius is able to infect Artemisia princeps, O. compositus, and Z. matrella but not Axonopus compressus, Eremochloa ophiuroides, Ophiopogon japonicus, or Cynodon dactylon. A. princeps plants wilted within 2 weeks postinoculation, but inoculated O. compositus and Z. matrella were asymptomatic, and P. noxius could be isolated from only inoculated sites. The colonization of P. noxius in the cortex and vascular cylinder of roots was visualized by paraffin sectioning and trypan blue staining of juvenile seedlings grown on water agar. To evaluate the effect of replantation for the remediation of BRRD-infested sites, P. noxius-inoculated wood strips were buried in soil with or without vegetation. After 4 weeks, P. noxius could be detected only in the bare soil group. For the control of BRRD, the herbaceous hosts should be removed around the diseased trees/stumps and non-host turfgrasses (e.g., A. compressus, E. ophiuroides, O. japonicus, or C. dactylon) planted to accelerate the degradation of P. noxius.

Effect of continuous lighting on the growth and leaf chemical components of Artemisia princeps grown hydroponically in a plant factory condition

Young leaves of Artemisia princeps Pamp. (Japanese mugwort), already used as a foodstuff in Japan, can be positioned as a functional health food because of remarkably higher contents of chlorogenic acid and total polyphenol compared to common vegetables. To procure young leaves in demand on a year-round basis by hydroponic production in fully artificial light-type plant factories, we investigated whether 24-h photoperiod, known to enhance some beneficial constituents, could improve the growth and chemical constituents of Japanese mugwort plants grown hydroponically in a plant factory condition. As we previously demonstrated that lowering the nutrient solution concentration increased chlorogenic acid and total polyphenol contents of the leaves without reducing the growth, plants were cultivated with a lower concentration of nutrient solution. The results indicated that it is possible to grow Japanese mugwort hydroponically under 24-h photoperiod in a plant factory condition with a nutrient solution concentration as low as 25% of the standard. In addition, under 24-h photoperiod, plant growth was greatly accelerated and chlorogenic acid as well as total polyphenol were increased, suggesting that 24-h photoperiod is highly beneficial for Japanese mugwort production in a fully artificial light-type plant factory.

The altered production and property of saliva induced by ingesting fermented food ingredients affect the oral microbiome composition in mice.

Oral functions are diverse and critical to human health. Therefore, insufficient secretion or poor quality of saliva, which is secreted into the oral cavity and plays various roles, could have a crucial influence on the oral microenvironment and be associated with systemic disease development. Here, we investigated the effects of food ingredients on saliva quantity and quality, including fermented ones. Through the in vitro submandibular glands' organ culture analyses, we found that "Yomo gyutto," fermented Japanese mugwort (Artemisia princeps), altered the expression of aquaporin-5, a water channel protein. We also found that Yomo gyutto increased saliva volume, along with the amount of α-amylase in mice, and caused changes in the oral microbiome composition of mice. These results suggested that by ingesting Yomo gyutto, we could directly and effectively manipulate the quantity and quality of saliva secreted from the salivary glands, potentially altering the oral microbiome composition for individual health.

Protective Role of Eupafolin against Tumor, Inflammation, Melanogenesis, Viral Disease and Renal Injury: Pharmacological and Analytical Aspects through Scientific Data Analysis

Background: Plant derived natural products have been always used in medicine for the treatment of human disorders due to their pharmacological activities and safety profile. Plant derived natural products have been used in food, Nutraceuticals and medicine for the better growth and development. Phytoconstituents are pure chemicals derived from various plant material and some of the best examples are tannins, alkaloids, terpenes, terpenoids, coumarins, polysaccharides, glycosides, flavonoids and phenols. Flavonoids are pure phytochemicals present in plants and their derived products and examples are eupafolin, kaempferol, quercetin, apigenin, myricetin, and luteolin. Flavonoids have different pharmacological activities due to their unique chemical structure and ring substitution. MethodS: In the present investigation, various scientific databases, including PubMed, Google, Science Direct and Scopus, have been searched to collect all the needed information of eupafolin. The biological potential and pharmacological activities of eupafolin have been investigated in the present work through literature data analysis of eupafolin through different scientific works. Detailed pharmacological activities of eupafolin have been collected and analyzed in the present work to know their effectiveness in the medicine for the treatment of human disorders. The importance of analytical techniques for the separation, identification and quantification of eupafolin in medicinal plants has been also investigated in the present work through literature data analysis. Results: Analysis of scientific databases revealed that eupafolin is a flavonoid class phytochemical found to be present in Arnica chamissonis, Arnica Montana, Artemisia mongolica, Artemisia princeps, Artemisia scoparia, Chamaemelum nobile, Dimerostemma vestitum, Eupatorium cannabinum, Eupatorium perfoliatum, Gaillardia aristata, Kalanchoe brasiliensis, Kalanchoe pinnata, Korscheltellus gracilis, Lantana montevidensis, Lippia canescens, Lippia dulcis, Phyla nodiflora and Salvia officinalis. Scientific research data analysis revealed the biological importance of eupafolin in medicine due to its anti-tumor, anti-inflammatory, anti-viral and anti-oxidant activity. However its potential on melanogenesis, cardiomyocytes, cerebral ischemia/reperfusion (I/R) injury, glutamate release has been also mentioned in the scientific research work. The study of analytical data revealed the importance of Gas chromatography, GC-MS, HPLC-DAD, HPLC-UV, TLC and UHPLC-MS for qualitative and quantitative analysis of eupafolin in the medicine and other allied health sectors. Conclusion: Eupafolin has a very impressive pharmacological profile and could be used against different types of human disorders.

Distribution Strategies of Vascular Plants at the Reclaimed Lands at the Saemangum Sea Dike

The distribution of plant communities in the reclaimed land of the southwestern coasts of South Korea, along with the environmental or plant factors, was studied through canonical correspondence analysis (CCA) and the competitor (C), stress tolerator (S), and ruderal (R) (CSR) ecological strategies. The coastal reclaimed land plants were classified into three plant-factor groups in the CCA biplot diagram. Axis1 was correlated with LS, SLA, CH, and FS. Axis 2 was correlated with LDMC, FP, and LDW. The reclaimed landplants were classified into three soil-factor groups in the CCA biplot diagram. First, the group factor was correlated with SAND. Second, the group factor was distributed according to T-N and TOC. Third, the group factor was distributed according to Salinity content. To clarify the relative significance of competition, stress, and disturbance in the distribution process of plant communities, the CSR distribution model was adopted. Sixteen of the 19 species were allocated to the factors related to the ruderals strategies such as R/CR and CR including R/CSR, SR/CSR, and CR/CSR, and SC strategies. They displayed ruderal and competition adaptation strategies reflecting the ecological environment. Seven species showed R/CR (ruderal/competitor-ruderal) strategies; Aeschno meneindica, Chenopodium album var. centrorubrum, Lolium perenne, Lotus corniculatus var. japonicus, Phragmites communis, Portulaca oleracea, and Soncous brachyotus and 6 species showed CR (competitor-ruderal) strategies; Bidens frondosa, Echinochloa crus-galli, Echino chloaoryzicola, Erigeron canadensis, Fimbristy lislongispica and Setaria viridis. The three species with R-related strategies were Artemisia princeps, Lolium perenne, and Trifolium repens. The distribution patterns of the CCA diagrams and CSR triangles may provide a useful scientific basis for protecting and restoring reclaimed lands and their valuable ecosystem services, from the increasing disturbances.

A Narrative Review of Human Clinical Trials on the Impact of Phenolic-Rich Plant Extracts on Prediabetes and Its Subgroups.

Phenolic-rich plant extracts have been demonstrated to improve glycemic control in individuals with prediabetes. However, there is increasing evidence that people with prediabetes are not a homogeneous group but exhibit different glycemic profiles leading to the existence of prediabetes subgroups. Prediabetes subgroups have been identified as: isolated impaired fasting glucose (IFG), isolated impaired glucose tolerance (IGT), and combined impaired fasting glucose and glucose intolerance (IFG/IGT). The present review investigates human clinical trials examining the hypoglycemic potential of phenolic-rich plant extracts in prediabetes and prediabetes subgroups. Artemisia princeps Pampanini, soy (Glycine max (L.) Merrill) leaf and Citrus junos Tanaka peel have been demonstrated to improve fasting glycemia and thus may be more useful for individuals with IFG with increasing hepatic insulin resistance. In contrast, white mulberry (Morus alba Linn.) leaf, persimmon (Diospyros kaki) leaf and Acacia. Mearnsii bark were shown to improve postprandial glycemia and hence may be preferably beneficial for individuals with IGT with increasing muscle insulin resistance. Elaeis guineensis leaf was observed to improve both fasting and postprandial glycemic measures depending on the dose. Current evidence remains scarce regarding the impact of the plant extracts on glycemic control in prediabetes subgroups and therefore warrants further study.

5-Desmethylsinensetin isolated from Artemisia princeps suppresses the stemness of breast cancer cells via Stat3/IL-6 and Stat3/YAP1 signaling

AimsTo study 5-desmethylsinensetin exhibiting potential anticancer activity against breast cancer stem cells and the related molecular mechanism. Main methodsIn this study, isolation of a cancer stem cell (CSC) inhibitor of Artemisia princeps was performed using a silica gel column, a Sephadex gel column, and high-performance liquid chromatography. A single compound was purified via activity-based isolation using mammosphere formation assays. An MTS was used to examine the proliferation of breast cancer cells, and flow cytometry was used to analyze apoptosis and cancer stem cell markers. Western blotting was used to detect the signaling pathway. ResultsThe isolated compound was identified as 5-desmethylsinensetin using nuclear magnetic resonance and mass spectrometry. 5-Desmethylsinensetin suppresses the proliferation and mammosphere formation of breast cancer cells, reduces the subpopulations of CD44+/CD24- and ALDH1+ cancer cells, and reduces the transcription of the stemness markers Oct4, c-Myc, Nanog and CD44 in Breast CSCs. 5-Desmethylsinensetin inhibits the total and nuclear expression of Stat3 and p-Stat3, as well as the translocation of YAP1. Additionally, 5-desmethylsinensetin reduces the mRNA and protein levels of IL-6. ConclusionOur results show that 5-desmethylsinensetin exhibits potential anticancer activity against breast cancer stem cells via Stat3-IL-6 and Stat3-YAP1 signaling.

Complete genome sequence of artemisia virus B, a new polerovirus infecting Artemisia princeps in South Korea.

The complete genome sequence of a new polerovirus found naturally infecting Artemisia princeps, artemisia virus B (ArtVB), was determined using high-throughput sequencing. The ArtVB genome comprises 6,141 nucleotides and contains six putative open reading frames (ORF0 to ORF5) with a genome structure typical of poleroviruses. A multiple sequence alignment showed that the complete ArtVB genome shares 50.98% nucleotide sequence identity with ixeridium yellow mottle virus 1 (IxYMaV-1, GenBank accession no. KT868949). ArtVB shares the highest amino acid sequence identity in P0 and P3-P5 (21.54%-51.69%) with other known poleroviruses. Phylogenetic analysis indicated that ArtVB should be considered a member of a new species within the genus Polerovirus, family Luteoviridae.

Effects of seasonal light variation and artificial light treatments on growth and flavonoid production of Artemisia princeps cultivated in greenhouses

Artemisia princeps (Ganghwa wormwood) is a medicinal plant that produces two major flavonoids, eupatilin and jaceosidin, which are used in the treatment of gastritis and peptic ulcers. A. princeps is primarily field cultivated, which has some drawbacks, including only one cultivation period per year and variations in flavonoid production due to environmental changes. The objective of this study was to analyze the effects of seasonal light variation and artificial light treatments on the growth and flavonoid production of A. princeps grown in greenhouses for year-round production. The plants were cultivated and harvested nine times in one year under natural seasonal light conditions in greenhouses. During the winter growth period (when natural light is substantially lower), four artificial light treatments were applied during two cultivation periods, from September 2016 to January 2017: supplemental light, night interruption, low light, and low light with night interruption. The plants grown under the natural light condition in greenhouses were used as a control. After harvest, the growth of the plants was measured, and the contents of eupatilin and jaceosidin were determined. The plants had the highest biomass when the accumulated radiation and duration were highest. The growth and flavonoid production were significantly associated with accumulated radiation and light duration. The supplemental light and night interruption treatments resulted in significantly higher biomass and flavonoid production, with the night interruption treatment requiring less energy input than the supplemental light treatment. Therefore, for consistent biomass and flavonoid production of A. princeps, a night interruption treatment is suggested in greenhouse cultivation during low irradiation and short days (less than 13 h).

Effect of Nutrient Solution Concentration on the Growth and Leaf Chemical Constituents of Hydroponically Grown Ibuki-yomogi, an Indigenous Line of Artemisia princeps in Shiga Prefecture

Effect of Nutrient Solution Concentration on the Growth and Leaf Chemical Constituents of Hydroponically Grown Ibuki-yomogi, an Indigenous Line of <i>Artemisia princeps</i> in Shiga Prefecture