Background: Trichinellosis caused by the gastrointestinal nematode Trichinellaspiralis occurs in humans, domestic animals and wild animals. It is difficultto control the muscle phase of the parasite. Homeopathic drugs such as Cinaand Santoninum have anthelmintic properties. We have observed that in materialdoses, the homeopathic drug Podophyllum also has nematotoxic properties.We have also observed that homeopathic potency can influence thewater permeability of cells. Objective: The purpose of this study was to investigatewhether potentized homeopathic drugs such as Cina 30, Santoninum30 and Podophyllum mother tincture can affect the muscle phase ofthe parasite T. spiralis in mice. Another objective was to see whethertrichinellosis and its treatment with the 3 named homeopathic drugs couldalter the water content in the muscle tissue of mice. Materials and Methods:Cina 30 and Santoninum 30 were prepared from the mother tincture of theflowering tops of Artemisia nilagirica and its active principle santonin, in eachcase by successive dilution (1:100) with 90% ethanol and sonication in 30steps following the single glass method (K30). Ethanol 30 was prepared bysuccessive dilution of 90% ethanol with 90% ethanol (1:100) followed by sonicationin 30 steps. In each step, the dilution was sonicated at 20 KHz for 30 s.We have observed before that sonication is a more uniform, measurable andeffective process of mechanical agitation of a liquid than manual succussion.Experimentally infected mice were orally treated with an aqueous Podophyllumsuspension at 60 mg/kg/day. Each potentized drug was diluted 1:20 withdistilled water and administered orally at 0.05 ml/mouse/day. Each mousewas inoculated with T. spiralis larvae at a dose of 200 larvae/mouse byesophageal intubation. Treatment was started on day 7 post-infection andcontinued for 120 days. After completion of treatment, the mice were sacrificedand the larvae were extracted from muscles by HCl-pepsin digestion.The water content of the muscles was measured by determining the differencebetween fresh weight and dry weight of the tissue. Results: Podophyllum?, Cina 30 and Santoninum 30 reduced the larval population in the studiedmice by 68.14%, 84.10% and 81.20%, respectively, as compared to the untreatedcontrol group. Ethanol 30 achieved no significant reduction in the larvalpopulation compared to the untreated control group. The water content ofthe muscle tissue in the untreated control group and the Podophyllum-treatedgroups was significantly higher than in the Ethanol 30-, Cina 30- and Santoninum30-treated groups. Conclusions: (1) Podophyllum ?, Cina 30 andSantoninum 30 were effective in the muscle phase of T. spiralis infection andsignificantly reduced the larval population in the treated mice. The potencieswere more effective than the mother tincture, an effect which was not dueto the medium ethanol. (2) The potencies significantly reduced the watercontent of the muscle tissue which might have affected the larvae. The effectof Podophyllum ? might be due to the direct toxic effect of the drug on thelarvae.