Determination Of Arsenic Research Articles

Arsenic aggravates oxidative stress causing hepatic alterations and inflammation in diabetic rats

AimsStudies have shown that exposure to either environmental toxicants or hyperglycemia causes hepatic injuries. However, it is unclear the extent to which their combined exposure may influence liver functions. Therefore, we aimed to evaluate morphological and functional hepatic parameters in diabetic rats exposed to arsenic. MethodsDiabetes was induced in male rats by intraperitoneal streptozotocin injection. While healthy and diabetic animals received saline solution (negative control and diabetes control, respectively), other animals received 10 mg/L sodium arsenate (arsenic control and diabetes + arsenic groups, respectively) for 40 days in drinking water. Liver tissue was subjected to antioxidant enzymes analysis, cytokine assay, arsenic determination, and histopathological evaluation. Functional markers of hepatic damage were analyzed using serum samples. Key findingsArsenate exposure reduced the antioxidant enzymes activity in healthy rats, and it worsened the reduction of GST in diabetic animals. Consequently, arsenate-exposed animals showed increased malondialdehyde and carbonyl protein levels, being this increase worsened in diabetes + arsenic animals. Arsenate-exposed groups also showed hepatic inflammatory process with high number of mast cells and TNF-α production mainly in diabetes + arsenic animals. Vascular alterations, such as congestion, bleeding, and hemosiderin deposition were intensified in diabetes + arsenic animals, whereas glycogen storage reduced in these animals. SignificanceWe concluded that arsenate exposure was able to intensify morphological and functional damages in liver tissue of diabetic animals.

Speciation of Arsenic in Ground Water Samples by Ion Exchange Chromatography with Improved Sample Preparation Technique.

Arsenic exists in the form of various chemical species differing in their physicochemical behavior, toxicity, bioavailability and biotransformation. The determination of arsenic species is an important issue for environmental, clinical and food chemistry. However, differentiation of these species is a quite complex analytical task. Numerous speciation procedures have been studied that include electrochemical, chromatographic, spectrometric and hyphenated techniques. Ion exchange chromatography is one of the techniques used to speciate inorganic arsenic i.e., arsenic (III) and arsenic (V). Both of these arsenic species are stable and exist as anions, arsenic (III) as arsenite (AsO33-) and arsenic (V) as arsenate (AsO43-). The proposed method can be used to perform the speciation of arsenic (III) and arsenic (V). It is a two-step process, first arsenic (V) is determined, followed by total arsenic determination by treating the sample with potassium permanganate solution to oxidize the arsenic (III) present in the sample to arsenic (V) and determining it as arsenic (V). Addition of potassium permanganate solution to the ground water samples for oxidizing the arsenic (III) to arsenic (V) is the newly adopted sample preparation technique and further determining it by ion exchange chromatography with conductivity detection. This method can be used for arsenic speciation in water samples from rivers and under the ground. Analytical validation and performance of the method is also discussed.

A novel approach to converting alkylated arsenic to arsenic acid for accurate ICP-OES determination of total arsenic in candidate speciation standards

A new approach was developed for quantitatively converting alkylated arsenic to arsenic acid (AsV). Conversion of the organic As species to AsV was required to prevent bias in the ICP-OES measurements because the species in the calibration standard was AsV. The approach was used in the development of a new series of organic As reference materials, SRMs 3030, 3031, 3033 and 3034, for monomethylarsonic acid, dimethylarsinic acid, arsenobetaine and arsenocholine, respectively, at the National Institute of Standards and Technology. Trimethylarsine oxide (TMAO) was used as a model alkylated arsenic compound to assess the effectiveness of the conversion due to its structural stability and difficulty to convert to AsV. Hydrochloric acid (HCl) was the only reagent capable of quantitative conversion of the alkylated arsenic compound to AsV using microwave assistance. The minimum concentration of HCl required for quantitative conversion under the experimental conditions was 4.4 mol L−1, which was 3 mL of concentrated HCl in 8 mL of solution. This is the first report of HCl affecting the quantitative conversion of alkylated arsenic to AsV. After converting to AsV, the mass fraction of arsenic in the alkylated arsenate compounds was quantified using ICP-OES with an expanded uncertainty ≤ 2.4 %. These ICP-OES results were in good agreement with INAA, a technique that is not susceptible to interferences from elemental speciation.

Improvements in the electrochemical generation of arsine at gold electrodes as a sample introduction strategy for arsenic determination in well-water samples by atomic absorption spectrometry

ABSTRACTThe development and evaluation of a cathode consisting of a gold electrode covered by a polyaniline film, PANI/Au modified electrode, for electrochemical generation of arsine is described. The efficiency of arsine production using the new electrode was ascertained by comparison with corresponding hydride generation atomic absorption spectroscopy determinations of arsenic (As) in aqueous standards. The PANI/Au modified electrodes provide better sensitivity, accuracy and precision than the pure Au cathodes. The PANI/Au cathodes were prepared by controlled electrodeposition cycles of polyaniline films on top of Au electrodes. Cathodes obtained after 10 electrodeposition cycles showed the best performance, while the ones obtained after five voltammetry cycles produced films too thin and those obtained after 20 voltammetry cycles produced films too thick for the purpose of this work. Under optimised conditions, the As limit of detection in aqueous solutions, according to the (3σ) criterion, was 2.48 µg L−1. For accuracy determination, the modified electrode was applied to quantification of As(III) in acidified aqueous solutions of the certified standard reference material, NIST SRM 1643d, and in well-water samples containing possible interfering ions. Application of the Student’s t-test showed no significant difference between the expected and obtained results of the NIST SRM standard analysis at the 95% confidence level. Values for six replicate determinations of As(III) in the well-water samples showed close agreement with values obtained by analyses using hydride generation atomic absorption. Recoveries were in the range of 95–105% at test for acceptable accuracy in the analysis of real samples.

Evaluation of digital camera as a portable colorimetric sensor for low-cost determination of inorganic arsenic (III) in industrial wastewaters by chemical hydride generation assisted-Fe(III) − 1, 10-phenanthroline as a green color agent

This research presents a novel, simple and green method for colorimetric determination of As(III) using a digital camera as the colorimeter. It is based on chemical hydride generation of arsine (AsH3) from acidic solution of As(III) by NaBH4 as well as the sequential reaction of arsine with Fe(III)-1,10-phenanthroline solution that produces red complex of Fe(II)-1,10-phenanthroline. The intensity of color red is related to the concentration of As(III) and acquired by image processing—Image J—software. To achieve the best sensitivity, we investigated the changes of RGB value in terms of red color intensity of the complex. Blue was the best as it showed the highest sensitivity. Under optimized conditions, the calibration curve was linear in the range of 1–25 µg mL−1 for As(III) and detection limit was 0.392 µg mL−1. The relative standard deviation (RSD) for five replicate measurements of 10, 15, 20 µg mL−1 of As(III) were 0.89, 2.43 and 3.08%, respectively. The proposed method was successfully used to determine As(III) in the industrial wastewater using standard addition method. The desirable recovery values (96–109%) indicate applicability of the proposed method for determination of As(III) in a complex matrix, such as industrial samples, in the presence of several unknown interferences without the need for any sample preparation.

Determination of arsenic in agricultural soil samples using High-resolution continuum source graphite furnace atomic absorption spectrometry and direct solid sample analysis

Soils around coal-fired thermal power plants based on coal combustion can present high concentrations of arsenic. This fact has a direct effect on the food chain. Arsenic can be absorbed by plants and vegetables through the soil, which will then serve as food for different animals, spreading the contamination. A method has been developed using high-resolution continuum source graphite furnace atomic absorption spectrometry (HR-CS GFAAS) for direct determination of arsenic in solid soil samples. Different chemical modifiers were tested to suppress the matrix effects observed. Among them, the modifier that showed the best results was the Zr, used as a permanent modifier. The optimized pyrolysis and atomization temperatures were 1000 °C and 2200 °C, respectively. A calibration curve was established using aqueous standard solutions which was linear up to 16 ng of arsenic. The characteristic mass and limit of detection were 22 pg and 73 pg As, respectively. The accuracy of the method was verified using two certified reference materials and comparison with results obtained for samples after microwave-assisted digestion. Eleven soil samples were collected around the power plant Complex Jorge Lacerda–Tractebel Suezin, in the south of Santa Catarina, Brazil. The concentration of As ranged from 3.4 mg kg−1 to 9.7 mg kg−1, which is within the limits allowed by Brazilian legislation.

Anodic stripping voltammetric determination of total arsenic using a gold nanoparticle-modified boron-doped diamond electrode on apaper-based device.

A multistep paper-based analytical device (mPAD) was designed and applied to the voltammetric determination of total inorganic arsenic. The electrodeposition of gold nanoparticles on a boron-doped diamond (AuNP/BDD) electrode and the determination of total inorganic arsenic is accomplished with a single device. Total inorganic arsenic can be determined by first reducing As(V) to As(III) using thiosulfate in 1.0molL-1 HCl. As(III) is then deposited on the electrode surface, and total inorganic arsenic is quantified as As(III) by square-wave anodic stripping voltammetry the potential range between -0.25V and 0.35V (vs. Ag/AgCl), best at around0.05 V. Under optimal conditions, the voltammetric response for As(III) detection is linear in the range from 0.1 to 1.5μgmL-1 and the limit of detection (3SD/slope) is 20ngmL-1. The relative standard deviation at 0.3, 0.7 and 1.0μgmL-1 of As(III) are 3.6, 4.3 and 3.3, respectively (10 different electrodes). The results show that the assay has high precision, a rather low working potential, and excellent sensor-to-sensor reproducibility. The method was employed to the determination of total inorganic arsenic in rice samples. Results agreed well with those obtained by inductively coupled plasma-optical emission spectroscopy (ICP-OES). Graphical abstract A multistep paper-based analytical device (mPAD) is described thatintegrates a AuNP/BDD electrode preparation step and a detection step into a single device. The AuNPs are easily deposited on the BDD electrode by applying electrodeposition potential. The total inorganic arsenic concentration in rice samples wasdeterminedby using square-wave anodic stripping voltammetry.

Separate quontitative determination of organic and non-organic arsenic in sea products

Separate quontitative determination of organic and non-organic arsenic in sea products

Раздельное количественное определение органических и неорганических форм мышьяка в морепродуктах

Раздельное количественное определение органических и неорганических форм мышьяка в морепродуктах

Simultaneous determination of arsenic, cadmium and lead in plant foods by ICP-MS combined with automated focused infrared ashing and cold trap

A fully automated focused infrared lightwave ashing sample preparation system was proposed and applied to the pretreatment of volatile arsenic, cadmium and lead in plant foods prior to inductively coupled plasma mass spectrometry (ICP-MS) determination. All the steps of ashing pretreatment were automatically accomplished within forty minutes. Gold-plated infrared quartz tubes produced focused infrared to supply a rapid heating. Ozone was used to accelerate sample carbonization. A cool trap was used to capture volatile arsenic, cadmium and lead. Three certified plant food reference materials were determined. The recoveries of the three elements were between 90% and 107%. Five real plant foods were analyzed by the proposed method and microwave digestion. The results showed no significant difference.

Preconcentration and speciation of arsenic by using a graphene oxide nanoconstruct functionalized with a hyperbranched polyethyleneimine.

A column sorbent for arsenic was obtained through immobilization of highly branched polyethylenimine (PEI) on graphene oxide (GO). The composite material enables speciation of arsenic by tuning the pH of the sample solution which governs the surface charge of the sorbent, depending on whether amino groups (-NH2) are present (at high pH) or ammonium groups (-NH3+; at low pH). The composite can be applied to improved speciation of arsenic (compared to unmodified GO). There is no need for oxidation or reduction of arsenic. A column procedure was applied for the sequestered extraction and speciation of As(III) and As(V) from environmental water samples before their determination by hydride generation-microwave induced plasma-atomic emission spectrometry. The method has a preconcentration factor of 440 for As(III) and of 400 for As(V). The limits of detection (at 3S/N) are extremely low, being 1.8± 0.2 ngL-1 for As(III) and 1.3± 0.08 ngL-1 for As(V). This is much lower than the arsenic guideline value of 10 μgL-1 as given by the WHO. Graphical abstract Graphene oxide interconnected with polyethyleneimine has been employed for the speciation and determination of arsenic. Quantitation by atomic emission spectroscopy reveals a high preconcentration factor (440 and 400) and low LODs of 1.8 ± 0.2 and 1.3 ± 0.08 ngL-1for As(III) and As(V), respectively.

A new strategy for the modification of a carbon paste electrode with carrageenan hydrogel for a sensitive and selective determination of arsenic in natural waters

An adsorptive stripping voltammetric method for the determination of As(III) and Astotal in water samples using a carrageenan modified carbon paste electrode is presented for the first time (CAR−CPE). The modified electrode was prepared in different ways: by adding CAR in solid form or as a hydrogel together with graphite and paraffin, as well as adsorbing CAR by applying a potential on an unmodified carbon paste electrode. The best results were obtained when CAR was incorporated as hydrogel (HCAR−CPE). The selection of the ratio amounts for electrode preparation was carried out applying a multivariate experimental design. Variables like amount of graphite (U1), HCAR (U2) and paraffin (U3) were optimized using a (2K+2K+C) model. The results showed that the amount of HCAR was the most significant factor, and the adequate U1:U2:U3 ratio to prepare the electrode was: 493 mg of graphite, 214 μL of paraffin and 134 μL of carrageenan as gel. The optimum parameters for the determination of As(III) were pH = 3.25 (0.01 mol L−1 H3PO4/H2PO4− solution); Eacc = −0.50 V and tacc = 30 s. The electrode presents good linear behavior concentration range from 0.50 to 6.70 μg L−1, with a limit of detection of 0.22 μg L−1. The relative standard deviation was 5.0% at the 1.5 μg L−1 As(III) level (n = 16). The method was validated by quantifying As(III) in spiked tap water from laboratory (RE: 3.0%), and it was applied for the determination of Astotal in water samples from the Loa River (North of Chile) prior reduction of As(V) with Na2S2O3 solution, obtaining 814.00 ± 0.03 μg L−1. The results of the proposed method were compared with those obtained by adsorptive stripping voltammetry with HMDE and by Inductively Coupled Plasma Mass Spectrometry (ICP−MS) techniques.

Evaluation of straightforward and rapid multi-element analyses of stream sediments for geochemical mapping in the remote islands of Japan — Seto Inland Sea region —

The straightforward and rapid determination of 53 elements in stream sediments using ICP-AES (inductively coupled plasma atomic emission spectrometry), ICP-MS (inductively coupled plasma mass spectrometry), and AAS (atomic absorption spectrometry) were evaluated for the geochemical mapping of isolated islands. Samples of 0.1 g were decomposed with HF, HNO3, and HClO4 at 125–145°C for 3 h to improve the determination of elements, including refractory minerals. The concentrations of rare earth elements (REEs), Nb, and Ta increased by 5–15% on average, and those of Zr and Hf increased by 30% on average. For arsenic determination, 0.1 g samples were digested using a mixed acid solution with an oxidizing reagent (KMnO4) at 120°C for 20 min. Decomposition without using the oxidizing reagent and/or extended decomposition times has been shown to cause a decrease in As concentrations in geochemical reference materials. However, similar As concentrations in stream sediment samples were obtained irrespective of KMnO4 addition and decomposition time. AAS was used to Hg measurement after thermal decomposition of about 50 mg samples without pre-treatment. The estimated concentrations of 53 elements in geochemical reference materials measured using ICP-AES, ICP-MS and AAS were agreement with the recommended values. Thus, it is concluded that the precision and accuracy of the rapid and straightforward analysis for geochemical mapping were satisfactory.

Determination of Trace Elements in Infant Formulas Available on Polish Market.

The aim of this study was to assess the levels of 13 essential and toxic elements (As, Cd, Co, Cr, Cu, Fe, Mn, Mo, Ni, Pb, Se, V, Zn) in the infant formulas, available on Polish market. Selected food samples were of animal (cow- and goat-based milks) and plant (soy-based milk, hypoallergic products, grain porridges) origin. Two analytical techniques, inductively coupled plasma mass spectrometry (ICP-MS) and neutron activation analysis (NAA), have been complementarily applied to analyze elemental content of 16 formulas dedicated for infants between 0 and 8months. For arsenic determinations, the radiochemical mode of NAA was also used. The daily intake of some micronutrients in the age 0-8months for non-breastfed infants was estimated and compared with present safety limits. Certified reference materials (CRMs) have been used for the validation of the methods: Non-fat Milk Powder 1549 (National Bureau of Standards-NBS), Soya Bean Flour INCT-SBF-4 (Institute of Nuclear Chemistry and Technology-INCT), Rice Flour SRM 1568b (National Institute of Standards and Technology-NIST).

Using an integrated method for the determination of environmental TCLP arsenic for sulphide-rich mine tailing remediation in Ghana, West Africa

Toxicity characteristic leaching procedure (TCLP) is a versatile short-term leaching protocol used to estimate the release of toxic metals from waste prior to disposal to a repository. This paper uses the integrated TCLP leachate data from tailings, tailings dam monitoring borehole data, and acidity ratio (AR) range of sulphide-rich ores to simulate the environmental TCLP As in tailings at the AngloGold Ashanti Obuasi mine in Ghana. The aim was to incorporate long-term leaching characteristics of tailings to minimise the risk of TCLP As test failure. The mean As concentration and pH value are 2.26 mg/l and 5.7 in TCLP leachate, 0.35 mg/l and 6.7 in monitoring boreholes, and < 0.01 mg/l and 5.7 in control boreholes, respectively. The evaluation of the TCLP As data using a one-sample t test performed at 80% confidence interval has the upper confidence limit (UCL) of 2.41 mg/l; this value which constitutes the short-term characterised environmental TCLP As is below the USEPA criterion of 5 mg/l and, therefore, qualifies the waste as safe for disposal. Alternatively, TCLP leachate, borehole and AR data were integrated to simulate the long-term environmental TCLP As of 2.40 mg/l and pH value of 5.7, and As concentration and pH value of 0.01 mg/l and 6.7 in monitoring boreholes, respectively. Such laboratory simulations of TCLP As leaching aimed at achieving 0.01 mg/l in field monitoring data would provide a more robust predictive value for environmental management decision making due to long-term considerations.

Determination and speciation of ultratrace arsenic and chromium species using aluminium oxide supported on graphene oxide

Alumina supported on graphene oxide (Al2O3/GO) nanocomposite as new nanosorbent in dispersive micro-solid phase extraction (DMSPE) for As(V) and Cr(III) preconcentration is described. The crucial issue of the study is synthesis of novel nanocomposite suitable for sorption of selected species of arsenic and chromium. Al2O3/GO demonstrates selectivity toward arsenates in the presence of arsenites at pH 5 and chromium(III) ions in the presence of chromate anions at pH 6. The Al2O3/GO nanocomposite was characterized by scanning electron microscopy (SEM) transmission electron microscopy (TEM), powder X-ray diffraction (XRD) and the Raman spectroscopy. The maximum adsorption capacity calculated based on the Langmuir adsorption model were 43.9 mg g−1 and 53.9 mg g−1 for As(V) and Cr(III), respectively. The nanocomposite was used as solid sorbent in preconcentration of As(V) and Cr(III)_ions from water samples and their determination using energy dispersive X-ray fluorescence spectrometry (EDXRF). The As(V) and Cr(III) ions can be quantitatively preconcentrated from 25 to 100 mL aqueous samples within 5 min using DMSPE procedure and 1 mg of Al2O3/GO. The nanocomposite was also used for preparation of Al2O3/GO membrane. Then, As(V) and Cr(III)_ions can be retained under flow condition by passing analyzed solution through Al2O3/GO membrane. Under the optimized conditions, As(V) and Cr(III) ions can be determined with very good recovery (92–108%), precision (RSD 2.7–4.0%) and excellent limit of detection (0.02 ng mL−1 As and 0.11 ng mL−1 Cr). The accuracy of the method was studied by analyzing certified reference materials (NIST 1640a) and spiked real water samples.

Determination of Arsenic by Hydride Generation—Laser-Induced Breakdown Spectroscopy: Characterization of Interelement Interferences

ABSTRACTIn this study, interelement interferences were evaluated for the determination of arsenic in aqueous samples through laser-induced breakdown spectroscopy (LIBS) hyphenated with a hydride generation sample introduction system. Optimum instrumental and chemical parameters were selected and variation in LIBS signal intensity was recorded for As solution in the presence of comparable concentrations of interfering elements. No significant change in the signal intensity of As(I) 228.8 nm line was observed in the presence of alkali/alkali earth metals; however, the presence of hydride-forming elements has shown a noticeable decrease in the line emission strength of arsenic. The least variation in arsenic signal was observed in the presence of Ge, the most volatile of all. However, the signal has decreased to a greater extent in the presence of Sn, Sb, and Pb. The presence of interfering elements on electron temperature and electron number density of arsenic plasma has also been studied. Plasma temperatures calculated using both As and Ar emission lines in the Boltzmann equation were similar, being around 5000 K. The McWhirter criterion for stationary and homogenous plasmas was utilized for the establishment of the local thermodynamic equilibrium under the plasma conditions studied. Applicability of the technique for multielemental analysis of water samples was tested through spiking experiments. Arsenic signal showed 26% decrease in the multielemental mixture solution. LIBS is among a few atomic spectroscopic techniques that facilitate rapid and simultaneous multielemental analysis without extensive sample preparation steps. However, the analytical performance of the technique still requires more serious efforts to compete with other conventional techniques for routine analysis of environmental samples.

Determination of Selenium and Arsenic Ions in Edible Mushroom Samples by Novel Chloride-Oxalic Acid Deep Eutectic Solvent Extraction Using Graphite Furnace-Atomic Absorption Spectrometry.

In present study, we proposed the application of a deep eutectic solvent (DES) made up of choline chloride (ChCl) and oxalic acid (Ox) for the dissolution of different edible mushroom samples for the determination of selenium (Se) and arsenic (As) ions. Therefore, an innovative, green, novel, and inexpensive method based on ChCl-Ox as the DES was developed for the determination of Se and As ions in mushroom species by graphite furnace-atomic absorption spectrometry. The important analytical parameters were also optimized. The LODs for Se and As ions were found to be 0.32 and 0.50 µg/L, respectively. The LOQs for Se and As ions were found to be 1.06 and 1.65 µg/L, respectively. The RSD was observed to be less than 5% for both analyte ions. The accuracy of the developed method was confirmed by analyzing mushroom powder Certified Reference Material CS-M-3 (Boletus edulis). The developed technique was effectively useful for the determination of Se and As ions in different species of mushroom samples from Turkey.

Simultaneous Determination of Arsenic, Cadmium, Calcium, Chromium, Cobalt, Copper, Iron, Lead, Magnesium, Manganese, Molybdenum, Nickel, Selenium, and Zinc in Fertilizers by Microwave Acid Digestion and Argon Inductively Coupled Plasma-Optical Emission Spectrometry Detection: Single-Laboratory Validation, First Action 2017.02.

A single-laboratory validation method for the simultaneous determination of nutrient and non-nutrient metals in commercial fertilizers was reviewed by the AOAC Expert Review Panel (ERP) and Stakeholder Panel for Fertilizers at the 2017 midyear AOAC INTERNATIONAL meeting. In addition to using 15 of the original 30 fertilizer materials from the previous Method 2006.03 collaborative study, NIST Standard Certified Reference Material 695 and Magruder check sample 2009-06 were also incorporated as accuracy, recovery, and repeatability materials. Method 2017.02 is suitable for the simultaneous determination of arsenic, cadmium, calcium, chromium, cobalt, copper, iron, lead, magnesium, manganese, molybdenum, nickel, selenium, and zinc in all major types of commercial fertilizer products by microwave digestion and inductively coupled plasma-optical emission spectroscopy analysis. With recoveries ranging from 92.8 to 103.2% for analytes above the LOQs, improved RSDs, and enhanced efficiency as compared with the previous method, Method 2006.03, the ERP recommended this method for First Action Official MethodsSM Status.

A comparison of the determination and speciation of inorganic arsenic using general HPLC methodology with UV, MS and MS/MS detection

The determination and speciation of arsenic in natural resources such as drinking water and agricultural soils has been a growing concern in recent years due to its many toxicological effects [1–3]. To speciate and quantitate concentrations of <1 ppm of arsenic, typically an ion chromatograph (IC) interfaced to an inductively coupled plasma mass spectrometer (ICP-MS) is employed [4–9]. This methodology may be very robust and sensitive, but it is expensive and not as ubiquitous as high performance liquid chromatography (HPLC) with ultraviolet (UV) absorbance detection or electrospray ionization mass spectrometry (ESI-MS). Anion exchange chromatography is a well-documented means of speciating arsenite (As(III), As2O3) and arsenate (As(V), AsO4) using UV [10], conductivity [11], or ESI-MS detection [12,13]. This paper demonstrates the utilization of common liquid chromatographic instrumentation to speciate and determines inorganic Arsenic compounds using UV or MS via selected ion recording (SIR) or multiple reaction monitoring (MRM) detection. This paper describes the analysis of arsenite and arsenate samples prepared using both deionized and ground water. The limit of quantitation for the techniques described in this paper for samples spiked in ground water were 454 ppb (As(III)) and 562 ppb (As(V)) for UV detection, 45.4 ppb (As(III)) and 56.2 ppb (As(V)) for SIR detection, and 4.54 ppb (As(III)) and 5.62 ppb (As(V)) for MRM detection.