Aromatic Nitrogen Research Articles

Enhancement of oil forensic methodology through the addition of polycyclic aromatic nitrogen heterocycle biomarkers for diagnostic ratios

Current oil spill forensic identification of source oils relies upon hydrocarbon biomarkers resistant to weathering. This international technique was developed by the European Committee for Standardization (CEN), under EN 15522–2 Oil Spill Identification guidelines. The number of biomarkers have expanded at pace with technological advances, while distinguishing new biomarkers becomes more challenging due to interference of isobaric compounds, matrix effects, and high cost of weathering experiments. Application of high-resolution mass spectrometry enabled exploration of potential polycyclic aromatic nitrogen heterocycle (PANH) oil biomarkers. The instrumentation showed reduction in isobaric and matrix interferences, allowing for identification of low-level PANH and alkylated PANHs (APANHs). Weathered oil samples, obtained from a marine microcosm weathering experiment, enabled comparison with source oils to identify new, stable forensic biomarkers. This study highlighted eight new APANH diagnostic ratios that expanded the biomarker suite, increasing the confidence for identifying highly weathered oils back to their source oil.

Microbiome of High-Rank Coal Reservoirs in the High-Production Areas of the Southern Qinshui Basin.

To study the distribution features of microorganisms in distinct hydrological areas of the southern Qinshui Basin, C-N-S microorganisms were studied using 16S RNA sequencing, metagenome sequencing and geochemical technologies, showing the high sensitivity of microorganisms to the hydrodynamic dynamics of coal. The hydrodynamic intensity of the #3 coal gradually decreased from the runoff areas to the stagnant areas. The stagnant zones have higher reservoir pressure, methane content, δ13CDIC and TDS and lower SO42-, Fe3+ and NO3- concentrations than the runoff areas. C-N-S-cycling microorganisms, including those engaged in methanogenesis, nitrate respiration, fermentation, nitrate reduction, dark oxidation of sulfur compounds, sulfate respiration, iron respiration, chlorate reduction, aromatic compound degradation, denitrification, ammonification and nitrogen fixation, were more abundant in the stagnant areas. The relative abundance of C-N-S functional genes, including genes related to C metabolism (e.g., mcr, mer, mtr, fwd and mtd), N metabolism (e.g., nifDKH, nirK, narGHI, nosZ, amoB, norC and napAB) and sulfur metabolism (e.g., dsrAB and PAPSS), increased in the stagnant zones, indicating that there was active microbiological C-N-S cycling in the stagnant areas. The degradation and fermentation of terrestrial plant organic carbon and coal seam organic matter could provide substrates for methanogens, while nitrogen fixation and nitrification can provide nitrogen for methanogens, which are all favorable factors for stronger methanogenesis in stagnant areas. The coal in the study area is currently in the secondary biogenic gas generation stage because of the rising of the strata, which recharges atmospheric precipitation. The random forest model shows that the abundance of C-N-S microorganisms and genes could be used to distinguish different hydrological zones in coal reservoirs. Since stagnant zones are usually high-gas-bearing zones and high-production areas of CBM exploration, these microbiological indicators can be used as effective parameters to identify high-production-potential zones. In addition, nitrate respiration and sulfate respiration microorganisms consumed NO3- and SO42-, causing a decrease in the content of these two ions in the stagnant areas.

Dearomative triple elementalization of quinolines driven by visible light

Organoboron and organosilicon compounds are used not only as synthetic building blocks but also as functional materials and pharmaceuticals, and compounds with multiple boryl and silyl groups are beginning to be used for these purposes. Especially in drug discovery, methodology providing easy stereoselective access to aliphatic nitrogen heterocycles bearing multiple boryl or silyl groups from readily available aromatic nitrogen heterocycles would be attractive. However, such transformations remain challenging, and available reactions have been mostly limited to dearomative hydroboration or hydrosilylation reactions. Here, we report the dearomative triple elementalization (carbo-sila-boration) of quinolines via the addition of organolithium followed by photo-boosted silaboration, affording the desired products with complete chemo-, regio-, and stereoselectivity. The reaction proceeds via the formation of silyl radicals instead of silyl anions. We also present preliminary studies to illustrate the potential of silaboration products as synthetic platforms.

Discovery of a potent olaparib-chlorambucil hybrid inhibitor of PARP1 for the treatment of cancer.

Introduction: Development of Poly (ADP-ribose) polymerase (PARP) inhibitors has been extensively studied in cancer treatment. Olaparib, the first approved PARP inhibitor, showed potency in the inhibition of both BRCA (breast cancer associated)-mutated and BRCA-unmutated cancers. Methods: Aiming to the discovery of olaparib analogs for the treatment of cancer, structural modifications were performed based on the scaffold of olaparib. In the first series, reduction of carbonyl group to CH2 led to decrease of PARP1 inhibitory activity. Preserving the original carbonyl group, molecules with potent PARP1 inhibitory activities were derived by introduction of hydrazide and aromatic nitrogen mustard groups. The synthesized compounds were evaluated in the in the PARP1 enzyme inhibitory screening, cancer cell based antiproliferative assay, cell cycle arrest and apoptosis studies. Results: It is remarkable that, molecule C2 with chlorambucil substitution, exhibited potent PARP1 inhibitory activity and a broad-spectrum of anticancer potency in the in vitro antiproliferative assay. Compared with olaparib and chlorambucil, molecule C2 also showed significant potency in inhibition of a variety of BRCA-unmutated cell lines. Further analysis revealed the effects of C2 in induction of G2/M phase cell cycle arrest and promotion of apoptosis. Discussion: Collectively, the olaparib-chlorambucil hybrid molecule (C2) could be utilized as a lead compound for further drug design.

Analysis of polycyclic aromatic hydrocarbons (PAHs) and their derivatives in biochar treated stormwater

Polycyclic aromatic hydrocarbons (PAHs), oxygenated PAHs (oxy-PAHs) and nitrogen heterocyclic polycyclic aromatic compounds (N-PACs) are persistent and semi-volatile organic compounds primarily formed due to incomplete combustion of organic material or, in the case of the derivatives, through transformation reactions of PAHs. Their presence in the environment is ubiquitous and many of them have been proven carcinogenic, teratogenic, and mutagenic. These toxic pollutants can therefore pose a threat to both ecosystem and human health and urges for remediation strategies for PAHs and derivatives from water bodies. Biochar is a carbon-rich material resulting from the pyrolysis of biomass resulting in a very porous matter with high surface area for an enhanced interaction with chemicals. This makes biochar a promising alternative for filtering micropollutants from contaminated aquatic bodies. In this work, a previously developed and validated methodology for the analysis of PAHs, oxy-PAHs and N-PACs in surface water samples was adapted for its utilization in biochar treated stormwater with special emphasis on scaling down the solid-phase extraction as well as including an additional filtering step for the removal of particulate matter in the media.•Optimized extraction method for PAHs, oxy-PAHs and N-PACs from stormwater treated with biochar.•Biochar strongly impacts the stormwater matrix and, therefore, additional steps are required in the extraction methodology.•Solid-phase extraction combined with GC–MS have been used to analyse PAHs, oxy-PAHs and N-PACs in stormwater treated with biochar.

Нефтепродукты для северных территорий на основе процесса замедленного коксования

Possible risks of summer and inter-season fuel overproduction at the Russian Federation oil refineries are reviewed. Comprehensive analysis of properties of coking light gas oil narrow fractions hydrotreated at hydrogen pressure of 80 atm is conducted. Dependences of changes in density, cloud point, chilling point, content of aromatic hydrocarbons, sulfur and nitrogen on weighting of fraction composition are considered. Based on the received data the components of jet fuel, winter and summer diesel fuel were compounded from narrow fractions. Received blends are analyzed for compliance with the requirements of corresponding GOSTs. The possibility to receive jet fuel, winter and summer diesel fuel from light coking gas oil at hydrogen pressure of 80 atm is shown.

Research on the adsorption of melamine for Cu(II) and Pb(II)

Melamine contains three aromatic nitrogen atoms and three free amino groups, which have great adsorption potential for heavy metallic ions in wastewater. The sorption performances of melamine for Cu(II) and Pb(II) ( They are denoted as Cu&Pb) were studied. Some major effects such as adsorption time, original concentration, and temperature on its adsorption performances were studied. The effects confirmed that melamine had true adsorption for Cu&Pb, and higher selectivity for the adsorption of Cu&Pb in a binary blended system. The adsorption amount of Pb(II) on melamine reached 4.91 mg/g at pH 5, adsorption equilibrium time of 12 h, and temperature of 55 °C, while the sorption amount of Cu(II) was only 2.70 mg/g, with a difference of about 1.8 times. The adsorption mechanism shows that the sorption of Cu&Pb on melamine follows the Lagergren quasi-second-order kinetic model. This result indicates that the mixture of Cu&Pb can be separated by stepwise processes from a binary mixed system.

Infrared spectra of isoquinolinium (iso-C9H7NH+) and isoquinolinyl radicals (iso-C9H7NH and 1-, 3-, 4-, 5-, 6-, 7- and 8-iso-HC9H7N) isolated in solid para-hydrogen.

Protonated polycyclic aromatic nitrogen heterocycles (H+PANH) are prospective candidates that may contribute to interstellar unidentified infrared (UIR) emission bands because protonation enhances the relative intensities of the bands near 6.2, 7.7 and 8.6 μm, and the presence of the N atom induces a blue shift of the ring-stretching modes so that the spectra of H+PANH match better with the 6.2 μm feature in class-A UIR spectra. We report the infrared (IR) spectra of protonated isoquinoline (the 2-isoquinolinium cation, iso-C9H7NH+), its neutral counterpart (the 2-isoquinolinyl radical, iso-C9H7NH), and another mono-hydrogenated product (the 6-isoquinolinyl radical, 6-iso-HC9H7N), produced on the electron-bombardment of a mixture of isoquinoline (iso-C9H7N) with excess para-hydrogen (p-H2) during matrix deposition at 3.2 K. To generate additional isomers of hydrogenated isoquinoline, we irradiated iso-C9H7N/Cl2/p-H2 matrices at 365 nm to generate Cl atoms, followed by IR irradiation to generate H atoms via Cl + H2 (v = 1) → HCl + H; the H atoms thus generated reacted with iso-C9H7N. In addition to iso-C9H7NH and 6-iso-HC9H7N observed in the electron-bombardment experiments, we identified six additional hydrogenated isoquinoline species, 1-, 3-, 4-, 5-, 7- and 8-iso-HC9H7N, via their IR spectra; hydrogenation on the N atom and all available carbon atoms except for the two sharing carbon atoms on the fused ring was observed. Spectral groupings were achieved according to their behaviors after maintenance of the matrix in darkness and on secondary photolysis at various wavelengths. The assignments were supported via comparison of the experimental results with the vibrational wavenumbers and IR intensities of possible isomers predicted using the B3LYP/6-311++G(d,p) method. The implications in the identification of the UIR band are discussed.

Molecular Target Interactions of Quinoline Derivatives as Anticancer Agents: A Review.

One of the leading causes of death worldwide is cancer, which poses substantial risks to both society and an individual's life. Cancer therapy is still challenging, despite developments in the field and continued research into cancer prevention. The search for novel anticancer active agents with a broader cytotoxicity range is therefore continuously ongoing. The benzene ring gets fused to a pyridine ring at two carbon atoms close to one another to form the double ring structure of the heterocyclic aromatic nitrogen molecule known as quinoline (1-azanaphthalene). Quinoline derivatives contain a wide range of pharmacological activities, including antitubercular, antifungal, antibacterial, and antimalarial properties. Quinoline derivatives have also been shown to have anticancer properties. There are many quinoline derivatives widely available as anticancer drugs that act via a variety of mechanisms on various molecular targets, such as inhibition of topoisomerase, inhibition of tyrosine kinases, inhibition of heat shock protein 90 (Hsp90), inhibition of histone deacetylases (HDACs), inhibition of cell cycle arrest and apoptosis, and inhibition of tubulin polymerization.

Use of Mixed Cultures for the Production of Grape–Plum Low-Alcohol Fermented Beverages

This work presents the attempt to develop a production technology for grape–plum low-alcohol beverages and enhance their chemical composition and flavor complexity through the non-Saccharomyces species. Saccharomyces cerevisiae (SC) pure cultures were used as reference beverages. Pure cultures of Lachancea thermotolerans (LT) and co-inoculated Lachancea thermotolerans with Saccharomyces cerevisiae (MIX) were included for grape–plum must fermentation at a pilot scale. The process involves two steps: a primary alcoholic fermentation in stainless steel tanks (F1) and a secondary fermentation in a bottle after dextrose syrup addition (F2). The chemical compositions of all beverages obtained in F1 and F2 were studied. Compared to SC, must inoculated with L. thermotolerans (LT and MIX) required four more days to complete the fermentation of sugars during F1. SC fermentation tended to have slightly higher pH and titratable acidity values and lower concentrations of total phenols. Final levels of aromatic precursor nitrogen and sulfur amino nitrogen were obtained more in SC than in LT and MIX. SC treatment had higher final levels of histidine, phenylalanine, isoleucine, lysine, methionine, threonine, valine, and cysteine. Related to individual amino acids, SC treatment had higher final levels of histidine, phenylalanine, isoleucine, lysine, methionine, threonine, valine, and cysteine. Analysis of the volatile composition showed that, compared with SC, MIX had the highest percentage of higher alcohols (3-methyl-1-butanol and 2-methyl-1-butanol) and acetates (isoamyl acetate and isobutyl acetate) which are associated with fruity and banana aromas. A decreasing trend in volatile fatty acids was observed in LT and MIX compared to SC. LT application, both in pure and mixed culture, significantly modified the values of the percentage of 5 of the 10 ethyl ester compounds analyzed. Finally, the sensory analysis showed that there were no significant differences, even though the non-Saccharomyces had a higher percentage of volatile metabolites. The results have shown that through this process an innovative and high-quality product was obtained: a low-alcohol beverage made from grapes and plums, which could be developed at an industrial level due to the increasing interest of consumers in this type of product.

Structural Analysis and Protein Binding of Cephalosporins.

Cephalosporins are a widely used subclass of β-lactam antibiotics that demonstrate variable protein binding independent of generation or antibiotic coverage. Prior work analyzed carbon3 (C3) and carbon7 (C7) substituents (locations of R2 and R1 groups respectively) for protein binding interactions. This study builds upon these results with statistical analysis of additional agents of the class. Chemical structures of 23 cephalosporins were used to identify the presence of 40 functional groups, and correlative relationships were identified using established protein binding data. Four functional groups were significantly correlated with protein binding: tetrazole (positive association), pyridinium, primary amine, and quaternary amine (negative associations). Cephalosporins with a negative charge at physiological pH were associated with increased protein binding. Analysis of tetrazole-containing cephalosporins and ceftriaxone indicates the need for further study of the potential role in protein binding of neutral or negatively charged aromatic nitrogen heterocycles linked at the C3 position by a thiomethylene group.

The catalytic effect of alkali and alkaline earth metals on the transformation of nitrogen during phenylalanine pyrolysis

Effects of alkali metals (KCl, NaCl) and alkaline earth metals (CaO, MgCl2) on the evolution of nitrogen-containing compounds and product distribution during pyrolysis of phenylalanine were studied in this study. TG-IR was used to monitor the change of nitrogen-containing gases (NH3, HCN, HNCO) with temperature and their final releasing amounts. Using M06-2X method and 6-31g(d) basis group, the pyrolysis reaction paths of phenylalanine were simulated, and the influence mechanism of AAEMs on the evolution process of nitrogen-containing compounds was explored from the molecular level. The results show that KCl promoted the release of HCN and HNCO and inhibited the release of NH3; however, NaCl inhibited the production of NH3 and HCN, promoting the production of HNCO. Both CaO and MgCl2 promoted the release of HCN and NH3 and inhibited the release of HNCO, while MgCl2 had a minor effect on HNCO. Theoretical simulations reveal that the main reason for affecting the total amount of three nitrogen-containing gases is the competitive effect of AAEMs on the formation paths of aromatic nitrogen compounds. KCl and CaO inhibited the formation paths of aromatic nitrogen compounds, while NaCl and MgCl2 function conversely.

Comparative performance of theoretical tools in order to quantify the effect of the electric potential on the vibrational wavenumbers and intensities of the SERS of 2‐methylpyrazine adsorbed on a nanostructured silver electrode

AbstractThe effect of the electrode potential in surface‐enhanced Raman scattering (SERS) intensities and wavenumbers of 2‐methylpyrazine (2MP) was analyzed on the basis of a resonant charge transfer (CT) mechanism by using a simple theoretical model in which the metallic surface and its charge density were simulated by atomic silver clusters of different size (n) and charge (q), [Agn]q. Two linear silver atoms (n = 2) with zero charge (q = 0) and three linear silver atoms (n = 3) with positive and negative charges (q = ±1) linked to the two nonequivalent aromatic nitrogen atoms in 2MP were taken into account. The wavenumber shifts of the most intense bands and the SERS‐CT spectra of these two types of metal‐adsorbate supermolecule, [Agn‐N1]q and [Agn‐N4]q, were calculated by using a time‐dependent density functional theory (TD‐DFT) method and the independent mode displaced harmonic oscillator (IMDHO) approximation. A comparison of the effect of different levels of calculation, ab initio/DFT, on the predictions from the two theoretical models (isolated adsorbate/supermolecule) is also performed. Only DFT theoretical results of the metal‐adsorbate supermolecule allow to explain the main role of the pair of bands assigned to totally symmetric ring‐stretching 8a,b modes. The 8a vibration is the strongest band at any electrode potential, whereas the 8b mode reaches a maximum enhancement at −0.50 V and then decreases at −0.75 V. This model of a charged metal‐adsorbate interface allows for detecting the presence of a CT mechanism in a SERS record.

Application of High-Performance Liquid Chromatography with Fluorescence Detection for Non-Polar Heterocyclic Aromatic Amines and Acridine Derivatives Determination in Pork Loin Roasted in a Roasting Bag.

Heat treatment of meat can lead to the formation of carcinogenic organic compounds. The influence of dried fruits on the formation of non-polar heterocyclic aromatic amines (carbolines) and nitrogen derivatives of polycyclic aromatic hydrocarbons (azaarenes) in roasted pork loin was elucidated. Two hundred grams of fruit per 1 kg of meat were used as stuffing. Carbolines, derivatives of pyridoimidazole and pyridoindole, and azaarenes (benzoacridines and dibenzoacridines) were determined by means of high-performance liquid chromatography with fluorescence detection. The total concentration of six δ-, γ- and α-carbolines in roasted pork loin was 1.3 ng/g. This content decreased by 64%, 58%, and 54% in pork loin stuffed with prunes, apricots, and cranberries, respectively. Concentrations of β-carbolines (harmane and norharmane) increased under the influence of added fruits. The norharmane content increased the most, from 2.2 ng/g in the control sample to 12.3 ng/g in meat prepared with cranberries. The harmane content increased from 1.0 ng/g to 3.6 ng/g in meat with prunes. The total concentration of azaarenes (two benzoacridines and dibenzo[a,c]acridine), which was close to 0.1 ng/g, decreased in dishes with prunes and apricots by 54% and 12%, respectively. Azaarenes were not found in samples of meat stuffed with cranberries.

Selective hydrogenation and defunctionalization of heavy oil model compounds using an unsupported iron catalyst

Developing practical, inexpensive catalysts for the partial upgrading of crude oil is essential for converting heavy oil to meet pipeline standards without diluent. Here we report that air-stable unsupported iron sulfide nanoparticles derived from a single-source homogeneous precatalyst, Fe2S2(CO)6, are highly effective catalysts for the partial hydrogenation of substituted anthracenes and aromatic nitrogen and sulfur heterocycles. The reactivity of the anthracene series is governed by stereoelectronic effects imposed by the phenyl substituents, decreasing in the order anthracene > 9-phenylanthracene > 9,10-diphenylanthracene. Nitrogen- and sulfur-containing heterocycles are partially hydrogenated, with limited heteroatom removal for benzothiophene, showing that the unsupported iron sulfide catalyst is well-suited for the selective partial hydrogenation and limited defunctionalization, which are key for catalytic partial upgrading of heavy petroleum. The unsupported iron sulfide nanoparticles also catalyze hydrogenation of mixed “feeds” comprising combinations of model compounds, demonstrating that carbocyclic and heterocyclic molecules can be processed simultaneously with little self-inhibition of overall activity, a key requirement for compositionally challenging bitumen feeds. Several techniques were used to characterize the iron sulfide nanoparticles, as well as the organic reaction products. The heterogeneous catalyst, generated in situ, was more active than a ball-milled commercial iron sulfide catalyst. The heterobimetallic precatalyst Co2FeS(CO)9 does not enhance the rates of hydrogenation.

Mathematical Modeling of the Vacuum Gas Oil Hydrotreatment

Thermochemical properties of molecules and thermodynamic characteristics of vacuum distillate hydrotreatment were calculated by quantumchemical methods. A kinetic model of the hydrotreatment process was developed using a formalized transformation scheme of hydrocarbons. The developed kinetic model was employed in numerical studies aimed to estimate the effect of the feedstock composition on the residual content of heteroatomic components in the product of vacuum gas oil hydrotreatment, the effect of temperature on the content of aromatic hydrocarbons, nitrogen and sulfur in the hydrotreatment product, and the effect of the hydrogen-containing gas consumption on the content of sulfur and hydrogen sulfide in the hydrotreated vacuum gas oil.

Characteristics of humic acids in sod-podzolic soil under long-term exposure to different fertilization systems

The elemental composition and structure of humic acids in the soddy-podzolic heavy loamy soil of the Cis-Urals were studied during long-term application of organic, mineral and organo-mineral fertilizers under conditions of a long-term stationary experiment. Mineral fertilizers contributed to the formation of humic acids with increased content of aliphatic components, the ratio H : C = 1.45 and a high degree of oxidation (ω = 0.41). The low content of nitrogen in humic acids (from 2.0 to 2.6 at.%), with various fertilizer systems, is primarily due to its low content in the plant biomass of crops cultivated in crop rotation.The organic fertilizer system resulted in the enrichment of the structural aggregates of humic acids with aromatic groups and nitrogen. The high intensity of absorption of the >C=O group of carboxylic acids in the region of 1 717 cm-1 and double bonds of carbon atoms at 1 627 cm-1 confirms this position. Humic acids of the “manure 10 t/ha” and “manure 5 t/ha + equiv. NPK” have the largest number of carboxyl groups in their composition. The vibrations of the >C=O group of carboxylic acids are most weakly expressed in soil humic acids with a mineral fertilizer system. Organo-mineral fertilizer system demonstrates intermediate results in terms of the content of the main constitutional elements in humic acids. Mineral fertilizers application reduces the amount and thermal stability of the structural components of humic acids, thereby contributing to the degradation of the most stable part of macromolecule, which can lead to the loss of potential soil fertility. Тhe use of mineral fertilizers on sod-podzolic heavy loamy soil led to the enrichment of humic acids with aliphatic fragments, and of organic ones – increased the content of stable components of aromatic nature, but lowered their thermal stability. According to thermographic analysis the ratio of weight loss in the low-temperature zone and the weight loss in the high-temperature zone (Z) during long-term use of mineral fertilizers is 0.9, i. e. the proportion of components of aliphatic nature in the structure of humic acids increases in comparison with unfertilized or manure fertilized soil.

Pyrazole, imidazole and triazole: In silico, docking and ADMET studies against SARS-CoV-2

The Coronavirus pandemic, Covid-19 and SARS-Cov-2 put multidisciplinary research by chemists, biologists, pharmacists and theorists necessary and primordial task to find new active biomolecules which will be beneficial for all humanity. The azoles drugs are electronic rich, they should be used with caution, and an understanding of their pharmacokinetic profile, safety, absorption, distribution, excretion, metabolism, toxicity, and drug-drug interaction profiles isimportantto provide effective and cure therapy. In these objectives and goals, twenty aromatic nitrogen heterocycle compounds were chosen for in silico, docking and AMET studies against SARS-CoV-2. In this paper with respect to the protein S of SARS-CoV-2 properties, the GAUSSIAN 09w program used in the semi-empirical method at the AM1 level with the optimization of the geometry of the structures. Then Toxicity and physicochemical properties were evaluated by AMET. Molecular docking investigations conducted; the binding affinities as well as interactions of the sieve compounds with the SRAS-CoV-2 protein Spike using PyRx software. In general, the preliminary results are fructuous and needs further in vitro testes.

ON ASSESSING THE MORBIDITY OF THE POPULATION ASSOCIATED WITH THE ATMOSPHERIC AIR QUALITY ON THE EXAMPLE OF A RUSSIAN CONSTITUENT ENTITY

According to the World Health Organization (WHO), 24% of the global burden of diseases and 23% of all deaths associate with environmental factors. The presence of chemical impurities in the air can have an adverse effect on the health of the population. A connection has been established between the increased content of chemical impurities in the air and the development of such pathologies as diseases of the respiratory system, circulatory system, the formation of malformations, etc. Many countries implement projects to improve air quality. For the purpose of targeted development of management decisions aimed at minimizing the adverse impact of atmospheric air on the health of the population, the study of the morbidity of the population associated with the impact of priority atmospheric air hazard factors is relevant. The research goal is to characterize the morbidity of the population associated with air quality (on the example of Krasnoyarsk Krai) to identify the priority factors of atmospheric air that form the greatest contribution to the associated morbidity. The qualitative characteristics of the atmosphere and the level of morbidity of the population in Krasnoyarsk Krai are estimated on the basis of official data of federal and industry statistics. The analysis of the primary morbidity of the population, designated by the WHO as an indication of the effect of environmental factors, has been carried out. The authors calculate the number of third-party cases of diseases associated with the quality of the atmosphere and determine the priority risk factors. High proportions of air samples that do not meet the standards for the content of heavy metals, benz(a)pyrene, xylenes (59% of samples) have been registered over the territory of Krasnoyarsk Krai. Since 2012, there has been an increase of 0.2%–6.4% in the indicators of primary morbidity, indicated by the WHO as an indicator of the effect of environmental factors and a significant increase in congenital anomalies (the growth rate is 93.2%). Up to 231 thousand third-party cases of diseases of the general population with diseases affecting the respiratory organs, circulatory system, hematopoietic organs, nervous system, eyes and their appendages determine the presence of aromatic hydrocarbons, nitrogen dioxide, hydroxybenzene (as well as its derivatives), benz(a)pyrene, nitrogen oxide, ammonia, dihydrosulfide and carbon disulfide, sulfur dioxide in the air in concentrations exceeding the standards. The most substantial number of diseases associated with air quality forms in the class of respiratory diseases (2020 – 587.4 cases per 100 thousand population, 64.5% of the total air-associated morbidity).

Potential utilization of vitamin C industrial effluents in agriculture: Soil fertility and bacterial community composition

The potential of industrial effluents from vitamin C (VC) production was assessed for agricultural applications by monitoring plant growth, soil properties, and microbial community structure. The results demonstrated that two types of effluents—residue after evaporation (RAE) and concentrated bacterial solution after ultrafiltration (CBS)—had positive effects on the yield and VC content of pak choi. The highest yield and VC content were achieved with a combined RAE-CBS treatment (55.82 % and 265.01 % increase, respectively). The soil fertility was also enhanced by the application of RAE and CBS. Nitrate nitrogen and organic carbon contents in the soil were positively correlated with the RAE addition, while ammonium nitrogen and available phosphorus were positively correlated with the CBS addition. The diversity of bulk and rhizosphere soil bacterial communities increased significantly after the addition of RAE-CBS. The abundance of Sphingomonas and Rhizobium significantly increased after the RAE-CBS treatment, which affected aromatic compound hydrolysis and nitrogen fixation positively. Changes in plant growth and soil fertility were closely related to the upregulation of functional gene expression related to C, N, and P cycling. RAE and CBS application exerted various positive synergistic effects on plant growth, soil fertility, and bacterial community structure. Consequently, the study results confirmed the potential of RAE and CBS application in agriculture. This study provides an innovative solution for utilizing VC industrial wastewater in agriculture in a resourceful and economically beneficial manner while alleviating the corresponding environmental burden.