A highly selective and facile sensing platform based on MOF-235 is fabricated and explored for the synchronous detection of dopamine and uric acid. The MOF-235 material is prepared via a one-step hydrothermal method. The sensing platform is fabricated via drop casting of MOF-235 dispersion on bare glassy carbon electrode (GCE). Then it is sealed using chitosan solution. The as-prepared MOF-235 material has large amount of unsaturated iron metal ions available for electrochemically redox-driven transformation. The aromatic moieties and the uncoordinated carboxylate groups of the MOF-235 are capable of obtain well-separated signals for dopamine (DA) and uric acid (UA), which is attributed to the π-π interactions, hydrogen bond and electrostatic action. These characteristics endow the excellent permselectivity and sensitivity for the MOF-235 modified electrode. The designed sensing platform exhibits linear response over concentration range from 10 to 90 μM for DA, and 10 to 90 μM for UA with the detect limit of 3.34 μM for DA and 3.46 μM for UA separately. To our knowledge, this is the first attempt that MOF-235 is explored the applications in electrochemical determination of medical small molecules. The implementing of MOF-235 in voltammetric detection offers new opportunities for the development of MOF materials based electroanalytical devices.