2-Phenylethanol (2-PE) is an important high-grade aromatic alcohol, which is widely used in the cosmetics, perfumery and food industries. However, 2-PE is mainly synthesized using a chemical route, which produces environmental pollution and harmful by-products. Screening of high-yielding wild-type strains has become an important goal for the future biosynthesis of 2-PE. In this study, a wild-type Wickerhamomyces anomalus was isolated from rice wine fermented mash. By optimizing the initial glucose and l-phenylalanine concentrations, 2630.7 mg/L of 2-PE was obtained in shaking flasks. The conditions of initial glucose and l-phenylalanine concentration, pH, and inoculation amount were optimized for 2-PE production with W. anomalus. Finally, based on the optimal conditions, the 2-PE titer reached 4,727.3 mg/L by a single-dose fed-batch strategy in a 5-L bioreactor. The results showed that the ability was expanded to harness the Ehrlich pathway for the production of high-value aromatics in aroma-producing yeast species.