This study showcases the importance of the remote sensing of biological aerosols for both homeland security and environmental protection. We demonstrate the application of a hyperspectral Laser Induced Fluorescence Light Detection and Ranging (LIF-LIDAR) system for the remote sensing of bioaerosols during the international S/K CHALLENGE II field campaign at the U.S. Army Dugway Proving Ground (DPG). The system employs UV photoexcitation at 266 nm and fluorescence spectral analysis in the range of 250–650 nm at a resolution of approximately 2 nm. A two-stage Microchannel Plate (MCP)-ICCD-based receiver capable of single photon-counting detection limit was used. During the campaign, four different bioaerosol simulants were studied in enclosed chambers and open space experiments at night and at distances of 0.5–2 km. A real-time Spectral Angle Mapping (SAM) algorithm was implemented to identify unknown bioaerosol clouds, dust, and combustion plumes as they spread in the open atmosphere. Additionally, we conducted preliminary tests in our institute, which demonstrated the system's ability to identify Ovalbumin (OV) aerosol despite being covered by a thick dust cloud. During the field measurements in DPG, we continuously tracked the fluorescence signature of OV aerosol as it spread in the atmosphere from a distance of 1.5 km for over 20 min. The overall detection performance during the S/K CHALLENGE II was 49 out of 52 trials, indicating a success rate of about 94%. The discussion centers on spectral identification statistics of the unknown dispersed bioaerosol as well as the implications of false-positive and false-negative events.
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