Brain acetylcholinesterase activity of the American pronghorn antelope (Antilocapra americana) collected from the US Army Dugway Proving Ground, Utah

Abstract

Brain tissue was analyzed for acetylcholinesterase (AChE) activity from 24 American pronghorn antelope (Antilocapra americana) collected on the US Army Dugway Proving Ground (DPG) (latitude 40°13' 52" N, longitude 112°45' 01" W), Tooele County, Utah. Pronghorn antelope from DPG were evaluated against 26 pronghorn antelope collected in Wyoming. The mean AChE activity was significantly greater (P < 0.001) in the Wyoming control group (4.612 ± 0.193 µM/gm brain tissue/min) relative to DPG (4.032 ± 0.621 µM/gm brain tissue/min). The DPG database exhibited a fourfold greater coefficient of variation, a tenfold greater variance, and a threefold increase in the standard deviation when compared to control AChE activity. Furthermore, the 95% confidence interval for the control and for the DPG data were not overlapping; the entire control data set was greater than the mean DPG AChE activity. A post hoc sequential Bonferroni statistical procedure showed two significantly (P < 0.001) distinct subsets in the DPG data. Mean DPG Subset I AChE activity (4.528 ± 0.347 µM/gm brain tissue/min) was indistinct from the mean control AChE value (4.612 ± 0.193 µM/gm brain tissue/min). The mean DPG Subset II AChE activity (3.537 ± 0.387 µM/gm brain tissue/min) differed significantly (P < 0.001) from the mean control AChE activity. The sum of resulting α values from the multiple statistical tests did not exceed the selected α value of P < 0.05, validating the post hoc sequential Bonferroni statistical procedure. Pronghorn antelope represented by Subset II, experienced a 23.3% mean loss of AChE activity suggesting sub-lethal organophosphate (OP) exposure rather than a low level chronic environmental influence was experienced by a population subset of the DPG pronghorn antelope herd. The origin of the DPG sublethal OP exposure and its long-term effects are speculative.