Argan Research Articles

Assessing the health and yield of argan trees in Morocco’s unique ecosystem: a multispectral and machine learning approach

ABSTRACT This research is focused on developing an AI model that utilizes multispectral camera data from the Souss-Massa region. The model aims to estimate the yield and identify a range of diseases in Argan trees through meticulous on-field investigations. Initially, the work is focused on understanding the resistance of Argan plants against different diseases, based on non-irrigated and irrigated Argan trees as well as planted ones. The results show that disease resistance is high in the case of non-irrigated Argan trees and low in the case of irrigated trees. In addition, we conducted a detailed study of the Argan trees to provide a comprehensive view of the plant’s health. Utilizing machine-learning techniques, the yield estimation model suggests that it is possible to achieve up to 97% accuracy in yield estimation, processing data at an impressive rate of 33 images per second. After careful consideration and analysis, we have concluded that machine counting is the most suitable technique for Argan plants. Machine counting and disease detection offer high precision, fast and efficient data processing and cost-effectiveness. Additionally, it is less labour-intensive and can be easily integrated into the existing production process.

Diversity index of argania spinosa l. Skeels plant formation in the algerian western sahara

The argan (Argania spinosa) is a xerothermophilous tree, endemic in North-West Africa. In Algeria, it is found as wild in the northwest of the Hamada du Drâa of Tindouf (Algerian Western Sahara), where it is characterized by a great floristic diversity. Several indices were calculated in this study to have a better knowledge on the state of biological diversity of the argan tree ecosystem. It is characterized by a floristic richness composed of 123 taxa belonging to 38 families including the dominance of three major floristic families: Asteraceae, Brassicaceae and Amaranthaceae. Biologically, the study area is dominated by therophytic species. This therophytization is marked by a general invasion of annual species. The diversity index of Shannon-Weaver (4.42) associated with that of Pilou's equitability (0.843) are relatively high, which showed that the flora of the argan forest of Tindouf is highly diverse. The disturbance index is high (76%) which reflects a significant degree of disturbance of the plant formation and also a strong anthropozoic influence. Bangladesh J. Bot. 53(3): 447-457, 2024 (September)

Assessing the Impact of Aridity on Argan Trees in Morocco: Implications for Conservation in a Changing Climate

Assessing the Impact of Aridity on Argan Trees in Morocco: Implications for Conservation in a Changing Climate

Agronomic features of introduced Argan tree in eastern of Morocco and optimization of their propagation

Argania spinosa or argan is an endemic plant of Morocco that belongs to sapotaceae family. This species has significant ecological and socioeconomical impact in Morocco and is mainly recognized by argan oil that is very known for its nutritional quality and health benefits. Our study aims to elaborate an agronomical and pomological characterization of introduced argan trees in Oujda, Morocco (about 200 trees, 10 years old) , select high-performance clone heads based on their productivity and stability criteria, by measuring various productivity parameters over a tree years study period (fresh fruit production, almonds production and oil production), and successfully propagate them asexually using apical grafting technique in controlled condition ( phytotron ) with the aim of stabilizing the high-performance clone. The obtained results indicated the existence of high diversity in terms of the various pomological parameters assessed. Parallelly, productivity and profitability parameters, the average production of fresh fruits, almonds and oil per tree and per year was 8.62 kg, 0.253 kg and 0.133 L, respectively. While, the maximal production was 34.4 kg/year for fresh fruits production, 1.07 kg/year/tree for almonds, and 0.575 liter/year/tree oil produced. Regarding the propagation of these clones, the results showed that the grafting success rate depended on the mother stock and the season, with 100% success rate. The results obtained show that the argan tree is an adaptive species, and the most suitable grafting method for the argan tree is apical split grafting during the autumn season.

Plant biotechnology for the conservation of Argania spinosa (Skeels L): optimising propagation and sustainable valorisation

ABSTRACT Argania spinosa, an endangered species from southwestern Morocco, is studied for preservation through plant biotechnology. The research aims to optimise argan tree propagation to boost production, preserve heritage, and use residual species. In vitro seedlings are transferred to a modified Murashige and Skoog medium with various phytohormones. Six combinations of auxins and cytokinins are tested for germination. Results show that a liquid medium with activated charcoal from argan shells promotes stem elongation. Meta-topoline at 0.5 mg L−1, combined with naphthalene acetic acid (NAA) at 0.2 mg L−1, enhances shoot growth, leaf formation, and callogenesis. Adding benzylaminopurine (BAP) at 0.1 mg L−1 and indole-3-butyric acid (IBA) at 0.02 mg L−1 to the medium elongates stems by 4.3 cm after 21 days. A 2000 ppm IBA concentration is key for a 90% rooting rate. These findings underscore plant biotechnology’s role in preserving and propagating argan trees, improving resource management, and supporting sustainability.

Effects of container size and growing media on growth of argan (Argania spinosa) seedlings in Morocco

Background: Argan (Argania spinosa) is an important arid species in Morocco, hence the need to undertake nursery practices that promote its regeneration and sustainability in a vulnerable environment. This study aimed to examine the effect of container size and growing medium on morphological traits, biomass, as well as root morphology of one-year-old argan seedlings in the Marrakech region of Morocco. Methods: Three container sizes of 300, 400, and 500 cm3 were selected in which seven types of growing media with varying proportions of acacia compost, crushed cypress cones, Barbary thuja, eucalyptus capsule composts, and potting soil were used. Subsequently, measurements of plant growth variables and biomass, as well as identification of root deformations were carried out. Results: Our results show that container size and growth medium strongly influenced (p < 0.001) morphological traits and biomass of argan seedlings. Growth was highest in seedlings grown in the 500 cm3 container on substrates composed of both 100% acacia compost and 50% potting soil + 50% acacia compost, while it was lowest in seedlings in the 300 cm3 container with the compost composed of a mixture of equal proportions of cypress and Barbary thuja, and eucalyptus. While the main root deformities were hooked roots (14.1%) and root eccentricity (13.0%), seedlings with multiple deformities were by far the most common (38.4%). The size of the container did not seem to influence their occurrence, whereas about 35% of the deformations were observed in seedlings grown on substrates composed of mixtures of potting soil and acacia compost, cypress cones, and Barbary thuja and eucalyptus composts. Conclusions: This study shows the importance of adopting an adequate protocol to allow the regeneration of forest species with the necessary characteristics to survive the often-challenging environmental conditions prevailing not only in southern Morocco but also across most of the country.

Predicting the climatic suitability of non-native drought resistant trees in the face of desertification – A case study with Argania spinosa in the Iberian Peninsula

Climate change is driving a poleward shift in the optimal distributions of terrestrial organisms, including trees. Consequently, trees are expected to decrease in climatically unfavourable areas as their optimal climatic conditions shift poleward. This is the case of Mediterranean High Nature Value agroforestry systems in Iberian Peninsula, dominated by holm oaks (Quercus rotundifolia) and cork oaks (Quercus suber), which are expected to shift their future distribution northward. Failure to keep pace with climate warming poses a heightened risk of extinction for these tree species, as well as the loss of crucial ecosystem services they provide. A similar situation is found for argan trees (Argania spinosa) in the southwestern region of Morocco, where it also supports an agroforestry system. The optimum climate for these trees is also shifting northward, toward the Iberian Peninsula. As the argan tree thrives in more arid conditions with irregular precipitation, the future climatic conditions in the Iberian Peninsula may become favourable for the persistence of this tree. In light of this situation, the assisted colonization of the argan tree could potentially contribute to protecting and preserving the tree-associated ecosystem services in Iberian Peninsula. We conducted climate suitability modelling using maximum entropy approach, for both the current and future timeframes, for North of Morocco and Algeria (native range) and the whole Iberian Peninsula (non-native range). Accordingly, there appears to be a significant area with highly suitable climatic conditions (>80 %) for argan tree occurrence, covering approximately 6.7 % of the territory, over 38,000 km2. This area is projected to increase significantly in the future, covering 9–22 % of the territory, or about 55,000 and 127,000 km2. With this study, we aim to contribute to the discussion on the benefits of assisted colonization of argan trees into Iberian Peninsula, as well as potential drawbacks such as the risk of this species becoming invasive.

Combined Effect of Subsurface Water Retention Technology and Arbuscular Mycorrhizal Fungi on Growth, Physiology and Biochemistry of Argan Seedlings under Field Conditions.

The argan (Argania spinosa L. Skeels) ecosystem is severely degrading in arid and semi-arid lands due to climate change, particularly in terms of density loss and reforestation failure. Thus, it is important to adopt innovative effective sustainable practices to optimize the densification and reforestation success of the argan tree. The purpose of the present research was to investigate the combined effect of subsurface water retention technology (SWRT) and the use of native arbuscular mycorrhizal fungi (AMF) on edaphic, growth, physiological and biochemical parameters of field-grown argan seedlings in the Essaouira region, Morocco. In this experiment, one-year-old argan seedlings were transplanted in the absence and presence of biodegradable plastic and AMF. Our findings revealed that the application of SWRT enhanced soil profile moisture up to 640% at 40 cm depth compared to the control. The combination of this technology with AMF also improved soil fertility. Furthermore, the application of SWRT, with or without AMF, significantly enhanced argan seedling height (208 and 168%, respectively), stomatal conductance (54 and 33%, respectively), and chlorophyll fluorescence (21 and 20%, respectively). Similarly, the combined application of SWRT and AMF significantly improved protein and sugar content (36 and 57%, respectively), as well as antioxidant enzyme activities (peroxidase and polyphenol oxidase) and chlorophyll pigments content compared to the control. However, this treatment reduced malondialdehyde and H2O2 content in the argan leaves. As a summary, SWRT technology combined with AMF may be used as a valuable strategy to promote the success of argan reforestation and to limit soil erosion and desertification in arid and semi-arid climates.

Plant-Growth-Promoting Rhizobacteria Improve Seeds Germination and Growth of Argania spinosa.

Argania spinosa is among the most important species of the Moroccan forest in terms of ecological, environmental, and socio-economic aspects. However, it faces a delicate balance between regeneration and degradation in its natural habitat. Hence, the efforts to preserve and regenerate argan forests are crucial for biodiversity, soil quality, and local livelihoods, yet they face challenges like overgrazing and climate change. Sustainable management practices, including reforestation and community engagement, are vital for mitigating degradation. Similarly, exploiting the argan tree's rhizosphere can enhance soil quality by leveraging its rich microbial diversity. This approach not only improves crop growth but also maintains ecosystem balance, ultimately benefiting both agriculture and the environment. This enrichment can be achieved by different factors: mycorrhizae, plant extracts, algae extracts, and plant growth-promoting rhizobacteria (PGPR). The benefits provided by PGPR may include increased nutrient availability, phytohormone production, shoot, root development, protection against several plant pathogens, and disease reduction. In this study, the effect of rhizobacteria isolated from the Agran rhizosphere was evaluated on germination percentage and radicle length for Argania spinosa in vitro tests, growth, collar diameter, and branching number under greenhouse conditions. One hundred and twenty (120) bacteria were isolated from the argan rhizosphere and evaluated for their capacity for phosphate solubilization and indole acetic acid production. The results showed that 52 isolates could solubilize phosphorus, with the diameters of the solubilization halos varying from 0.56 ± 0.14 to 2.9 ± 0.08 cm. Among 52 isolates, 25 were found to be positive for indole acetic acid production. These 25 isolates were first tested on maize growth to select the most performant ones. The results showed that 14 isolates from 25 tested stimulated maize growth significantly, and 3 of them by 28% (CN005, CN006, and CN009) compared to the control. Eight isolates (CN005, CN006, CN004, CN007, CN008, CN009, CN010, and CN011) that showed plant growth of more than 19% were selected to evaluate their effect on argan germination rate and radicle length and were subjected to DNA extraction and conventional Sanger sequencing. The 8 selected isolates were identified as: Brevundimonas naejangsanensis sp2, Alcaligenes faecalis, Brevundimonas naejangsanensis sp3, Brevundimonas naejangsanensis sp4, Leucobacter aridicollis sp1, Leucobacter aridicollis sp2, Brevundimonas naejangsanensis sp1, and Staphylococcus saprophyticus. The results showed that Leucobacter aridicollis sp2 significantly increased the germination rate by 95.83%, and the radicle length with a value of 2.71 cm compared to the control (1.60 cm), followed by Brevundimonas naejangsanensis sp3 and Leucobacter aridicollis sp1 (2.42 cm and 2.11 cm, respectively). Under greenhouse conditions, the results showed that the height growth increased significantly for Leucobacter aridicollis sp1 (42.07%) and Leucobacter aridicollis sp2 (39.99%). The isolates Brevundimonas naejangsanensis sp3 and Leucobacter aridicollis sp1 increased the gain of collar diameter by 41.56 and 41.21%, respectively, followed by Leucobacter aridicollis sp2 and Staphyloccocus saprophyticus (38.68 and 22.79%). Leucobacter aridicollis sp1 increased the ramification number per plant to 12 compared to the control, which had 6 ramifications per plant. The use of these isolates represents a viable alternative in sustainable agriculture by improving the germination rate and root development of the argan tree, as well as its development, while increasing the availability of nutrients in the soil and consequently improving fertilization.

New Insights into Trace Element Accumulation in Kernels of Two Algerian Populations of Argan (Argania spinosa (L.) Skeels): An Endangered Endemic Tree.

Argan is one of the valuable oilseed trees in the Northwest Africa area, with important ecological and socioeconomic benefits; however, information underlying its elemental status remains absent . The current work was performed for the first time to quantify the mineral status of Argania spinosa kernels collected from two different geographic Algerian regions (Mostaganem and Tindouf). Elemental investigation (As, Br, Ca, Ce, Co, Cr, Fe, K, La, Na, Rb, Sb, Sc, Sm, Th, and Zn) was performed using neutron activation analysis (NAA). Two standard reference materials from the National Research Center for certified reference materials (China), namely, CRM-GBW 07605 (GSV-4 tea leaves), and the National Institute of Standards and Technology (USA), namely, NIST (SRM 1573a, tomato leaves), were used to ensure precision and validation of NAA measurements. A computational calculation of data including principal component analysis (PCA) and Pearson correlation coefficient (r) was applied. The extents of essential elements in Mostaganem kernels were found to be in the order of K (7185 μg g-1) > Ca (2079 μg g-1) > Na (205 μg g-1) > Fe (84.33 μg g-1) > Zn (73.41 μg g-1) > Cr (0.66 μg g-1) > Co (0.035 μg g-1), whereas the overall order of these elements in Tindouf kernels was K (7206 μg g-1) > Ca (3725 μg g-1) > Fe (114.69 μg g-1) > Zn (67.37 μg g-1) > Na (56.29 μg g-1) > Cr (0.54 μg g-1) > Co (0.11 μg g-1). PCA results revealed that Tindouf population was highly loaded with Br, Cr, Cu, Mn, Sr, V, and Zn, while Mostaganem population was associated only with Ce and Sm contents. These findings can be useful for making new formulations for cosmetic and culinary usage and contribute to the nutritional database and food safety.

Oxidative stability and nutritional quality of stored Linum usitatissmium L. and Argania spinosa L., oil blends: Chemical compositions, properties and nutritional value

Identification of the chemical compositions of fatty acids and tocopherols shows the high content of linum usitatissimum oil (LO) by linolenic acid 55.3735% and γ-tocopherol 570.927 mg/kg, while argania spinosa oil (AO) is known by the dominance of oleic acid 47.77% followed by linoleic acid 31.08% as well as tocopherols by γ-tocopherols 687.485 mg/kg and δ-tocopherols 51.035 mg/kg. This difference in compositions enables us to enrich the low-stability oil and monitor its behavior during storage at a specific time and under specific conditions. In this study, pure linum usitatissimum and argania spinosa oils extracted by cold pressing as well as their formulations at proportions of (LO: AO) respectively: (80:20; 60:40, 50:50; 40:60; 20: 80) were oxidized at 60 °C for 28 days of storage, during which time the pure oils and blends were assessed for oxidative stability by studying their different fatty acid and tocopherol profiles and physicochemical characteristics such as acidity, peroxide value and chlorophyll and carotenoid pigments, as well as nutritional indexes such as the atherogenic index (AI), the thrombogenic index (TI), and the hypocholesterolemic: hypercholesterolemic ratio (HH), ω3:ω6 ratio, also the oxidative susceptibility (OS), and oxidazability value (Cox), and total phenolic compounds (TPC).

Germination and histo-morphological features of argan (Argania spinosa (L.) Skeels) seeds

Germination and histo-morphological features of argan (Argania spinosa (L.) Skeels) seeds

Development of Allometric Equations to Determine the Biomass of Plant Components and the Total Storage of Carbon Dioxide in Young Mediterranean Argan Trees

Morocco’s argan trees, native to forests, are now cultivated in large orchards within the Argan Biosphere Reserve, transforming “Arganiculture”. These orchards are anticipated to bolster carbon storage, but their precise contribution to carbon storage remains unclear. This study introduces for the first time allometric equations for estimating biomass in different components of argan plants within orchards. A total of 89 plant individuals, aged 2 to 6 years, were collected. Their diameter and total height were measured. The biomass, carbon content, and biomass carbon stock of each component were determined. The best-fit allometric equation incorporates diameter, height, growing years, and root-to-shoot ratio to estimate total biomass (R2 = 0.95). The estimated total carbon biomass stock ranged from 0.01 to 0.82 t CO2 ha−1 for plants, at a density of 200 plants ha−1. Between 2021 and 2023, the average annual carbon sequestration was 0.20 t CO2 ha−1 year−1. This model offers valuable tools for use when species-specific equations during the establishment growing stage are unavailable, enhancing carbon sequestration quantification for more reliable results and informing climate change mitigation strategies. The allometric parameters serve as benchmarks for trees resembling the argan tree. The methodology could be adapted for other forest plants undergoing conversion to orchard cultivation.

Revealing the ecological footprint of Argan (Argania spinosa) derivatives: A comprehensive analysis of the carbon impact of Argan oil

Argania spinosa, commonly known as the Argan tree, assumes a crucial role in bolstering environmental sustainability by augmenting carbon storage in both biomass and soil. Despite its significant environmental contributions, there exists a conspicuous gap in scientific research and data pertaining to the carbon dynamics associated with Moroccan Argan forest ecosystems and their prized products, particularly Argan oil. To address this research gap, our study meticulously investigates the intricate details of the carbon footprint linked to Argan oil production within the "Argan Biosphere Reserve" in southern Morocco.This innovative research aims to deliver a comprehensive assessment of the carbon footprint within the Argan oil industry, conducting a thorough examination of 10 companies and 16 cooperatives actively involved in production. The foundation of this investigation rests on on-site interviews with leaders of production units, systematically gathering essential data for rigorous analysis.Employing the "Bilan Carbone" tool, we intricately calculated emissions, categorizing them into three scopes. The resulting carbon footprint outcomes were meticulously delineated across three distinct scenarios, shedding light on the nuanced dynamics of greenhouse gas emissions.In the first scenario, encompassing all scopes, including export-related emissions, companies displayed an average carbon footprint of approximately 10.17 kg CO2 eq.l−1, while cooperatives recorded 8.68 kg CO2 eq.l−1. The second scenario, excluding export-related emissions, unveiled companies with an average carbon footprint of 3.71 kg CO2-eq.l−1 and cooperatives with 6.32 kg CO2-eq.l−1. The third Scenario, concentrating solely on scopes 1 and 2, indicated companies with an average estimated carbon footprint of 1.31 kg CO2 eq.l−1, and cooperatives with approximately 3.01 kg CO2 eq.l−1.Delving deeper into the intricacies of emissions by scenario and oil-producing organizations, our findings underscore the substantial impact of electricity consumption on the overall carbon footprint of Argan oil. For companies, electricity consumption contributed between 10.84% (first scenario) and 66.97% (third scenario), while for cooperatives, the range was 26.97% (first scenario) to 75.57% (third scenario).To optimize the carbon footprint, our study recommends strategic interventions such as reducing electricity and butane consumption. Moreover, adopting sustainable practices, including leveraging photovoltaic electricity and synthetic gas from the pyrolysis of Argan byproducts, can significantly curtail the average carbon footprint of companies and cooperatives, charting a trajectory toward environmental sustainability. Specifically, a reduction of 37.05% and 40.75% (second scenario) and an impressive 71.66% and 77.01% (third scenario) for companies and cooperatives, respectively, could be achieved after a 30-year amortization period of photovoltaic panels.These findings not only contribute valuable insights into the environmental impact of Argan oil production but also pave the way for future in-depth studies and the formulation of sustainable pathways to optimize the carbon footprint of this critical industry.

New sustainable strategy for rehabilitating phosphate mining sites using phosphate industry by-products and sludge integrating Argan, Carob, and Olive trees

In semi-arid context, the development of anthroposol from phosphate industry by-products and sewage sludge could be a new approach for sustainable phosphate mining soils. The objective of this 2 years field study carried out at Benguerir phosphate mine, was to evaluate nutrients and heavy metals dynamics in the soil-plant system of three species planted on anthroposol consisting of a mixture of 65% phosphogypsum (PG), 30% phosphate sludge (PS), and 5% sewage sludge (SS). The experimental layout was a randomized complete block design with six replicates and three species: argan, olive, and carob. No fertilizer was applied to the different plots. Heights and diameters of the different species were measured. Species were pruned, and leaves and stems were separated. The nutrients (N, P, K, Ca, and Mg) and heavy metals (Cd, Cu, Cr, Pb, Ni and Zn) contents in both soil and plant part were determined. The results of the Pearson correlation showed significant correlations between height and diameter of the different species. The strongest correlation was observed for the argan tree. Furthermore, nutrient contents in the rhizosphere of species were significantly reduced, excepted Ca. Also, nutrient contents in leaves were significantly higher than those in the stems. In addition, the concentration of heavy metals in argan, olive and carob leaves, were below international standards. In semi-arid contexts, argan tree could be the best candidate for revegetating mining sites and this anthroposol (65% PG – 30% PS – 5% SS) could be a new approach to rehabilitate mining site on large scale.

Contribution to the characterization of the seed endophyte microbiome of Argania spinosa across geographical locations in Central Morocco using metagenomic approaches.

Microbial endophytes are microorganisms that live inside plants, and some of them play important yet understudied roles in plant health, growth, and adaptation to environmental conditions. Their diversity within plants has traditionally been underestimated due to the limitations of culture-dependent techniques. Metagenomic profiling provides a culture-independent approach to characterize entire microbial communities. The argan tree (Argania spinosa) is ecologically and economically important in Morocco, yet its seed endophyte microbiome remains unexplored. This study aimed to compare the bacterial and fungal endophyte communities associated with argan seeds collected from six sites across Morocco using Illumina MiSeq sequencing of the 16S rRNA gene and ITS regions, respectively. Bacterial DNA was extracted from surface-sterilized seeds and amplified using universal primers, while fungal DNA was isolated directly from seeds. Bioinformatics analysis of sequencing data identified taxonomic profiles at the phylum to genus levels. The results indicated that bacterial communities were dominated by the genus Rhodoligotrophos, while fungal communities exhibited varying degrees of dominance between Ascomycota and Basidiomycota depending on site, with Penicillium being the most abundant overall. Distinct site-specific profiles were observed, with Pseudomonas, Bacillus, and Aspergillus present across multiple locations. Alpha diversity indices revealed variation in endophyte richness between seed sources. In conclusion, this first exploration of the argan seed endophyte microbiome demonstrated environmental influence on community structure. While facing limitations due to small sample sizes and lack of ecological metadata, it provides a foundation for future mechanistic investigations into how specific endophyte-host interactions shape argan adaptation across Morocco's diverse landscapes.

Genome of Argania spinosa L.: insights into oil production and the tocopherol biosynthesis pathway

Argan (Argania spinosa L.) is a highly valued tree for its multiple uses as food and feed and for being linked to a broad range of benefits of pharmaceutical and cosmetic relevance. This multipurpose resource is becoming increasingly overused, which may disrupt the whole ecosystem’s sustainability. Due to the high socio-economic status of this tree, research interventions are needed to reverse the forest regressive trend, restore the disturbed ecosystem, and conserve genetic diversity. However, research on argan is restricted by the lack of accessible information on the genetic and genomic bases of the species, specifically a functional annotated genome. Herein, we report the reference transcriptome aided annotation of the argan tree genome using de novo gene prediction programs aided by homology information from different plants. The results of the genome annotation using AUGUSTUS were subsequently improved by performing RNA sequencing. A total of 62,590 gene loci could be identified with 82,286 isoforms and a BUSCO completeness of 91.7%. To gain insight into the agronomically important compounds in argan oil, a comparative genome analysis and ortholog identification was performed, followed by phylogenetic tree construction of the main biosynthesis genes. Among those are fatty acids and tocopherols, the latter being the main factor behind the increasing demands for argan oil. Our analysis is the initial step to provide breeders, geneticists, and the industries with adequate genomic information, facilitate improvement of economically important traits and to selectively adapt the tree to the increasing impact of climate change.

Traditional medicinal plants used for rheumatoid arthritis and immune system disorders treatment in the Casablanca-Settat region, Morocco: An ethnopharmacological study

Rheumatoid arthritis, classified as an immune system disorder, stands as a prevalent condition that presents considerable challenges to healthcare systems worldwide. This study was delving into the realm of traditional medicine to explore the use of medicinal plants for the treatment of rheumatoid arthritis and immune system disorders in the Casablanca-Settat region of Morocco. With a focus on ethnopharmacological insights, a comprehensive survey was conducted involving 372 participants, including herbalists and patients, to document the local knowledge and practices associated with these conditions. The findings revealed a noteworthy reliance on traditional medicinal knowledge, with a prominent role played by females across different age groups. A diverse spectrum of 88 plant species hailing from 45 distinct families was identified, where in the Myrtaceae, Oleaceae, and Zingibneraceae families garnered prominence as the most endorsed choices. Noteworthy among these, Zingiber officinale and Curcuma longa emerged as highly favored species, boasting robust anti-inflammatory and immunomodulatory attributes. The study also offers insight regarding preferences concerning administration methods, highlighting the salience of oral consumption and infusion as the predominant modes of preparation. Nevertheless, the observed variability in dosage utilization underscores the traditional therapeutic paradigmsintricacies. Geographic disparities were discernible as well, with certain species displaying a region-specific usage pattern, further enriching the understanding of local herbal practices. Moreover, the study identified endemic species such as Olea europaea, Teucrium polium, Argania spinosa, and Withania adpressa contributing to the safeguarding of the region's indigenous botanical heritage. In conclusion, this study contributes to a deeper understanding of the traditional management of RA and immune system disorders in the Casablanca-Settat region. The documented plant species, practices, and preferences offer valuable insights into potential sources of anti-inflammatory and immunomodulatory agents.

The Potential Role of Major Argan Oil Compounds as Nrf2 Regulators and Their Antioxidant Effects.

In recent years, research on the discovery of natural compounds with potent antioxidant properties has resulted in growing interest in these compounds due to their potential therapeutic applications in oxidative-stress-related diseases. Argan oil, derived from the kernels of a native tree from Morocco, Argania spinosa, is renowned for its rich composition of bioactive compounds, prominently tocopherols, polyphenols, and fatty acids. Interestingly, a large body of data has shown that several components of argan oil activate the nuclear factor erythroid 2-related factor 2 (Nrf2) pathway, playing a crucial role in the cellular defense against oxidative stress. Activation of this Nrf2 pathway by argan oil components leads to the increased expression of downstream target proteins like NAD(P)H quinone oxidoreductase (NQO1), superoxide dismutase (SOD), heme oxygenase 1 (HO-1), and catalase (CAT). Such Nrf2 activation accounts for several health benefits related to antioxidant defense, anti-inflammatory effects, cardiovascular health, and neuroprotection in organisms. Furthermore, the synergistic action of the bioactive compounds in argan oil enhances the Nrf2 pathway. Accordingly, the modulation of the Kelch-like ECH associated protein 1 (Keap1)/Nrf2 signaling pathway by these components highlights the potential of argan oil in protecting cells from oxidative stress and underlines its relevance in dietetic prevention and therapeutic applications. This review aims to provide an overview of how major compounds in argan oil activate the Nrf2 pathway, updating our knowledge on their mechanisms of action and associated health benefits.

Ethnobotany, traditional knowledge, and nutritional value of Argan (Argania spinosa (L.) Skeels) in Western Anti-Atlas of Morocco.

The Souss region in Morocco is known nationally and internationally for its essential knowledge of traditional herbal medicine and the cultural heritage of nutrition. The endemic species Argania spinosa (L.) Skeels is an important crucial plant used by the local population to treat many diseases and prepare some authentic foods. To identify the therapeutic uses of the Argan tree [Argania spinosa (L.) Skeels] and their benefits in preparing authentic foods, the survey was conducted using semi-structured questionnaires. We have achieved 450 interviews with traditional health practitioners and knowledgeable villagers. In the region of Chtouka Aït Baha and Tiznit (Western Anti-Atlas). In parallel with this survey, we collected some old local manuscripts from conventional practitioners in the region. The data obtained were analyzed using specific ethnobotanical indices such as Use Value (UV), Fidelity Level (FL), and Relative Frequency of Citation (RFC). In parallel, we analyzed the nutritional value of some authentic foods derived the argan oil (Amlou, Tagoulla, and Labsis). All 450 interviewees use the argan oil in food or for the preparation of their authentic foods derived (Amlou, Tagoulla, and Labsis); among them, 100 persons use Argan, in addition to food, in the treatment of various diseases with UV and RFC at 1.94 and 0.22, respectively. Among eight treated diseases by argan tree, the treatment of skin and subcutaneous diseases had a very highly significant value of the FL index (98%). Similarly, the analysis of the manuscripts collected in the study area revealed an ancient therapeutic use of the argan tree. The results also show that the Argan oil extracted from the seed is used to prepare authentic foods with significant nutritional value, especially Amlou.