Plant biotechnology for the conservation of Argania spinosa (Skeels L): optimising propagation and sustainable valorisation

Abstract

ABSTRACT Argania spinosa, an endangered species from southwestern Morocco, is studied for preservation through plant biotechnology. The research aims to optimise argan tree propagation to boost production, preserve heritage, and use residual species. In vitro seedlings are transferred to a modified Murashige and Skoog medium with various phytohormones. Six combinations of auxins and cytokinins are tested for germination. Results show that a liquid medium with activated charcoal from argan shells promotes stem elongation. Meta-topoline at 0.5 mg L−1, combined with naphthalene acetic acid (NAA) at 0.2 mg L−1, enhances shoot growth, leaf formation, and callogenesis. Adding benzylaminopurine (BAP) at 0.1 mg L−1 and indole-3-butyric acid (IBA) at 0.02 mg L−1 to the medium elongates stems by 4.3 cm after 21 days. A 2000 ppm IBA concentration is key for a 90% rooting rate. These findings underscore plant biotechnology’s role in preserving and propagating argan trees, improving resource management, and supporting sustainability.