Moderate Resolution Imaging Spectroradiometer (MODIS) Land Surface Temperature (LST) products are essential data sources for global and regional climate change research. Currently, several versions of the MODIS LST product have been released, yet the performance differences and uncertainties they introduce in land surface studies remain insufficiently addressed. To bridge this gap, this study focuses on four distinct versions of the LST product: MxD11A1 Collection 5 (C5), Collection 6 (C6), Collection 6.1 (C6.1), and MxD21A1 Collection 6.1 (MxD21). The spatial resolution of all product generations is 1 km, and the temporal resolution is 0.5 days. This study provides a comprehensive analysis of the errors arising from different generations of these products in various land surface process studies. The error assessment includes cross-comparisons between product versions and evaluations of the absolute errors generated. Absolute errors in evaluation data were collected from 13 surface sites within the Heihe Watershed Allied Telemetry Experimental Research (HiWATER) project during the period 2013–2018. Cross-validation results show that the largest difference between C5 and C6.1 occurs over bare land, with an RMSE of approximately 1.45 K, while there is no significant change between C6 and C6.1. MOD21 shows considerable variation compared to C6.1 at night across different land cover types, with RMSE over cropland exceeding 2 K. The temperature difference between MOD21 and C6.1 is more pronounced at night (2.01 K) than during the day (0.30 K). Validation results based on temperature indicate that C5 has greater uncertainty compared to C6, especially over bare land, where errors are 2.06 K and 1.06 K, respectively. Furthermore, MxD21 demonstrates significant day–night performance discrepancies, with an average bias of 0.10 K at night, while daytime errors over bare land can reach 2 K, potentially influenced by atmospheric conditions. Based on the research in this paper, it is possible to clarify the performance of different versions of MODIS products, reflecting the appropriateness of their past applications; on the other hand, it is recommended to prioritize the use of the MxD11A1 C6 and C6.1 products for monitoring and applications in bare soil areas to ensure higher accuracy. Furthermore, for day and night monitoring, it may be beneficial to alternate between the MxD11A1 and MxD21A1 products to fully leverage their respective advantages and enhance overall monitoring effectiveness.
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