In order to quantify the aesthetic attraction of visual elements of highway landscape space, the concept of visual attraction intensity of highway landscape space is proposed, and the factors and indicators of visual attraction elements are clarified. Based on the perspective of human factors, taking passengers as the main attraction, taking 30 landscape elements from three typical highway landscape scenes, namely mountain highway landscape, plain highway landscape and elevated highway landscape, as the research object. The real-time evaluation and data processing system of subjective landscape preference has been independently developed, and combined with the processing and analysis of human-machine-environment synchronous quantitative analysis technology and in-depth learning, a “subjective+objective” quantitative evaluation model of highway landscape visual element attraction intensity based on dynamic perception has been constructed, revealing the quantitative relationship between highway landscape visual attraction elements and attraction intensity, The quantitative method and action law of the attraction intensity of highway landscape and the dynamic aesthetic perception of the attraction subject is clarified. It is found that the change of highway planning alignment, high green view rate and low enclosure have positive effects on the subjective preference score of the landscape (0.690), and there is a significant positive correlation between green view rate and enclosure; the richer the landscape elements such as mountains, trees and water bodies, the stronger the visual attraction, but the complexity of the landscape elements increases the cognitive load of the area of interest. Among the physiological indicators, there are significant correlations between EEG and ECG (0.790), EDA and ECG (0.632), and subjective preference and passengers' eye-movement behavior and EDA; as well as the change pattern of attraction intensity based on the dynamic perception of tunnel area. The results show that the attraction intensity of visual elements of highway landscape space based on dynamic perception is consistent with human aesthetic preference, visual perception and physiological response, which verifies the scientificity, appropriateness and accuracy of eye-movement analysis method and physiological analysis method for quantitative evaluation of aesthetic preference of highway landscape.