The Natural Laboratory of Peat Swamp Forest Area known as the Center For International Co-operation in Management of Trofical Peatland (CIMTROP) University of Palangka Raya has been set as specific research area of tropical peat swamps since 1993 and has a 50.000 hectares total area. Natural Laboratory of Peat Swamp Forest Area – CIMTROP University of Palangka Raya is peat and sandy land and as a catchment water area, that is crucial to preserve and protect its ecosystem. This research used remote sensing and GIS technology for land cover monitoring utilize Sentinel-2 images. The purpose of this research was to determine (1) land cover changes in the Natural Laboratory/CIMTROP UPR in 2016 and 2021, (2) The rate of land cover change in the Natural Laboratory/CIMTROP UPR and (3) the causes of land cover change in the Natural Laboratory/CIMTROP UPR. Land cover analysis by interpreting Sentinel-2 images in 2016 and 2021 used Supervised Classification Technique. Based on results of the analysis, there were five land cover classes: forest, shurbs, brush, swamp grass and open land. The accuracy testing process performed uses a confusion matrix method to produce mapping accuracy values for each land cover class. Between 2016 to 2021, the change in forest class decreased from 31.809,40 hectares (67,37%) to 29.888,10 hectares (63,30%). Contrary to the shrub class, there was and increase of 1.762,30 hectares (3,73%) to 2.585,20 hectares (5,47%). The increase in shrub area was due to forest reforestation, degradation and deforestation from the forest and land fires which occurred in previors years.