Rainfall has a significant impact on urban population mobility, posing great challenges to traffic management and urban planning. An understanding of this influence from multiple perspectives is urgently needed. In this study, we devised a multiscale comparative research framework to explore the spatiotemporal effects of rainfall on taxi travel patterns, aiming to provide a new perspective on the investigation of rainfall’s impact on urban human mobility. More specifically, at the macroscopic scale, we computed taxi travel indicators across the entire study area and used kernel density estimates to observe the spatiotemporal distribution patterns influenced by rainfall. Subsequently, complex traffic networks were constructed by considering urban road intersections as nodes and combined with visualization methods to understand changes in taxi travel patterns visually at the microscopic level. We selected Wuhan City, a typical urban area in southern China with frequent rainfall, as the study area and used meteorological data along with a large volume of taxi spatiotemporal trajectory data for investigation. Results indicated a 4.16% decrease in weekly travel volume due to rainfall, with a 3.96% decrease on workdays and a 4.64% decrease on weekends. However, nighttime rainfall between 19:00 and 22:00 on weekdays increased the demand for taxi travel. Furthermore, the impact of rainfall on weekends exceeded that on workdays, restricting people’s mobility and leisure activities, resulting in reduced travel to recreational tourist spots and commercial pedestrian streets. Rainfall altered residents’ travel preferences to some extent, with more residents choosing taxis during rainy weather, which led to decreased transportation efficiency and increased traffic congestion. These findings contribute to a deeper understanding of the complex relationship between population mobility patterns and the urban ecological environment, providing valuable insights for planning resident travel and taxi dispatching under adverse weather conditions.