Organic Architecture Research Articles

MicroRNA-1 targets ribosomal protein genes to regulate the growth, development and reproduction of Schistosoma japonicum

Eggs laid by mature female schistosomes are primarily responsible for the pathogenesis of schistosomiasis and critical for transmission. Consequently, elucidating the mechanism of sexual maturation as well as egg production may lead to new strategies for the control of schistosomiasis. MicroRNAs (miRNAs) are involved in multiple biological processes including reproduction in many organisms, yet their roles have not been well characterized in schistosomes. Here, we investigated microRNA-1 (miR-1), which was downregulated gradually in both male and female Schistosoma japonicum after they reached sexually maturity. The expression of miR-1, as shown with quantitative reverse transcription PCR (qRT-PCR), was lower in the reproductive organs of adult females compared with the somatic tissues. Overexpression of miR-1 in adult worms destroyed the morphological architecture of reproductive organs and reduced the subsequent oviposition, which may be due to the activation of apoptosis pathways. Through in silico analysis, 34 potential target genes of miR-1 were identified, including five ribosomal protein genes, called rp-s13, rp-l7ae, rp-l14, rp-l11 and rp-s24e. In vitro dual-luciferase reporter gene assays and miRNA overexpression experiments further validated that these ribosomal protein genes were directly regulated by miR-1. In contrast to the gene expression of miR-1, qRT-PCR and in situ hybridization experiments demonstrated these ribosomal protein genes were enriched in the sexual organs of adult females. Using RNA interference to silence the ribosomal protein genes in different developmental stages in a mouse model system, we demonstrated that these miR-1 target genes not only participated in the reproductive development of S. japonicum, but also were required for the growth and survival of the parasite in the early developmental stages. Taken together, our data suggested that miR-1 may affect the growth, reproduction and oviposition of S. japonicum by targeting the ribosomal protein genes, which provides insights for exploration of new anti-schistosome strategies.

Isostructurality or Not: A Deep Insight into the Selective Formation of Spironolactone Solvates

Isostructurality is a potential tool to design crystals with tailored arrangements, especially for multicomponent systems, but research into its formation mechanism is still limited to date. Herein, a family of spironolactone (SPI) isostructural and non-isostructural solvates was investigated to explore the selective formation of isostructural crystals. The SPI framework, dominated by van der Waals forces, is robust and flexible, with different solvent molecules in its channels playing the same structural role. The formation of the SPI isostructural solvate is a delicate energy balance between the energy loss caused by the expansion of the SPI framework and the addition of SPI···solvent interactions. Besides the size and functional groups of solvent molecules, the directionality of the SPI···solvent interactions should also be carefully considered in the isostructurality-based crystal design. In addition, weak interactions seem to be regarded as potential design elements for organic host architectures.

ЛАТЕНТНІСТЬ ПРОТОДЕКОНСТРУКТИВІЗМУ В ПРОЕКТНОМУ КОНТЕНТІ ФРЕНКА ЛЛОЙДА РАЙТА

Analyzing the work of the most influential architects of the middle of the 20th century, including the American architect Frank Lloyd Wright, it is possible to declare the presence of ideas at that time, which were later designed and prescribed by trendy deconstructivist architects in the nineties and early 2000s. In the middle of the 20th century, F. Wright continued to develop the principles of organicity in architecture, supplementing them with avant-garde aesthetics. Comparison of the graphic and three-dimensional morphology of buildings, methods and means of expression of the content of the master of architecture of the 20th century Frank Lloyd Wright during the extreme period of his work with the pattern of trendy deconstructivists leads to conclusions about the presence of the aesthetics of proto-deconstructivism in his author's philosophy. In his seventies, Wright experiments with the forms and graphic language of bricolage, which naturally transform into his philosophy of organic architecture. The objects proposed to be attributed to the period of proto-deconstructivism were created by Wright over a period of almost twenty years between 1937 and 1959. By its inner essence, the graphoplastic language of the projects was sharply avant-garde, so it was not always understandable for contemporaries. Architectural historian, critic and publicist Rainer Behnem, in the introductory article to his book "A View of Modern Architecture: The Age of Masters", which was published during the postmodern era of the 80s, states the following "... in the last five years of his career, Wright created the meanest projects an old man could create” (Banham, 1975). Apologists of 20th century modernism Mies van der Rohe, Walter Gropius, Le Corbusier and Frank Wright, about whom Rainer Behnem wrote, felt in advance the stylistic flow that formed together with the avant-garde current and was defined by postmodern deconstructivism. When analyzing Wright's projects from the point of view of the presence in them of the aesthetics of proto-deconstructivism, similarities with recognized characteristic patterns of architecture of this style are revealed. Wright's author's philosophy about the naturalness, that is, the organicity of architecture, was proto-deconstructivist and was formed in parallel with global metaphysical trends.

A Universal Organocatalyst for Selective Mono-, Di-, and Triboration of Terminal Alkynes

Multiboronate compounds are valuable synthetic building blocks for the construction of complex organic architectures via cross-coupling chemistry at the C–B bonds. Hence, there has been a tremendous amount of work in the past decades to develop practical multiboration reactions. Many of the developed methods start from alkynes, the most versatile synthetic precursors for multiboronates, and use transition-metal complexes as catalysts. However, several drawbacks such as low efficiency, poor selectivity, and narrow substrate and catalyst scopes remain major challenges to be addressed in this research area. Herein, we report the development of a universal organocatalytic system to promote the mono-, di-, and triboration of terminal alkynes in a highly efficient and selective manner. The reaction outcomes can be manipulated at will by varying the catalyst loading, reagent stoichiometry, and reaction time. A combined computational and experimental mechanistic study offers interesting insights into the selectivity of these sequential reactions.

Precisely Designed Difunctionalized Cyclodextrin Produces a Solid-State Organic Porous Hierarchical Supramolecular Assembly.

Regioselective di-functionalization of a cyclodextrin allows hydrophobic domains to be directed in a geometrically controlled manner. This controlled orientation ultimately gives access to an original hierarchical assembly in the solid state. This assembly spans over three levels of hierarchy which are governed by synergistic host-guest inclusions, directed hydrophobic effect and hydrogen bonding. This combination of interactions precisely positioned in space through regioselective functionalization of a cyclodextrin creates a porous organic architecture.

Off-Lattice Coarse-Grained Model of Surface-Confined Metal–Organic Architectures

An off-lattice model of self-assembling surface-confined metal–organic nanostructures (SMONs) comprising tripod molecules has been developed. The model considers the directionality and saturability of coordination bonding. To parametrize the model, density functional theory methods have been used. Self-assembly of the SMONs has been simulated with Metropolis Monte Carlo method in the canonical ensemble. In this paper, we investigate how the directionality of metal–linker bonding and the linker/metal ratio affect the self-assembly of SMONs containing metal centers with different coordination numbers: M(II), M(III), and M(IV). The directionality of coordination bonding is determined by the functional group of the linker molecule and is defined in the model as the angular diameter of the interaction shell. Regardless of the preferred coordination number of the metal center, even a small change in the angular diameter significantly affects the structure of the SMONs. Depending on the angular diameter and composition of the metal–organic layer, various structures can appear. Using our model, we have simulated the self-assembly of the random porous (or defected honeycomb) and triangular structures, the set of hexagonal phases, amorphous structures of different densities, and metal–organic chains. Relative thermal stabilities of clusters of the main structures have been studied. The model reproduces correctly the main SMONs observed by scanning tunneling microscopy.

Influence of Simulated Microgravity on Mammary Epithelial Cells Grown as 2D and 3D Cultures.

During space travel, astronauts will experience a unique environment that includes continuous exposure to microgravity and stressful living conditions. Physiological adaptation to this is a challenge and the effect of microgravity on organ development, architecture, and function is not well understood. How microgravity may impact the growth and development of an organ is an important issue, especially as space flight becomes more commonplace. In this work, we sought to address fundamental questions regarding microgravity using mouse mammary epithelial cells in 2D and 3D tissue cultures exposed to simulated microgravity. Mouse mammary HC11 cells contain a higher proportion of stem cells and were also used to investigate how simulated microgravity may impact mammary stem cell populations. In these studies, we exposed mouse mammary epithelial cells to simulated microgravity in 2D and then assayed for changes in cellular characteristics and damage levels. The microgravity treated cells were also cultured in 3D to form acini structures to define if simulated microgravity affects the cells' ability to organize correctly, a quality that is of key importance for mammary organ development. These studies identify changes occurring during exposure to microgravity that impact cellular characteristics such as cell size, cell cycle profiles, and levels of DNA damage. In addition, changes in the percentage of cells revealing various stem cell profiles were observed following simulated microgravity exposure. In summary, this work suggests microgravity may cause aberrant changes in mammary epithelial cells that lead to an increase in cancer risk.

Inkjet‐Printed p‐NiO/n‐ZnO Heterojunction Diodes for Photodetection Applications

AbstractTransparent Conducting Oxides (TCOs) are an enticing family of optoelectronic materials which have been proven to increase efficiency when incorporated into perovskite light emitting diode (PE‐LED) and organic OLED architectures as transport layers. Solution‐processed metal oxide inks have already been demonstrated, although there is still a need for high‐quality inkjet‐printable metal oxide inks with a thermal post‐process below 200 °C. The set of inks in this work are adapted from low‐boiling point colloidal suspensions of metal oxide nanoparticles synthesized via flame spray pyrolysis. High quality, pinhole‐ and wrinkle‐free inkjet‐printed layers are obtained at low temperatures through vacuum oven post process, as proven by scanning electron microscopy. The crystallinity of the layers is confirmed by X‐ray diffraction, showing the expected hexagonal and cubic structures respectively for ZnO and NiO. The thin film layers reach over 70% (ZnO) and 90% (NiO) transparency in the visible spectrum. Their implementation in the inkjet‐printed p–n diode shows excellent I–V rectifying behavior with an ON/OFF ratio of two orders of magnitude at ±3 V and a forward threshold voltage of 2 V. Furthermore, the device exhibits an increase in photocurrent around four orders of magnitude when illuminated under a 1‐sun solar simulator.

Molecular Identification, Pathogenesis, and Life Cycle of Sarcocystis cruzi from Cattle (Bos taurus) in New Valley Governorate, Egypt.

Sarcocystis cruzi was identified by molecular methods from an intermediate host, cattle (Bos taurus), in El-Kharga, New Valley Governorate, Egypt, and its life cycle and pathogenicity were studied in the final host, dogs (Canis familiaris). 600 slaughtered cattle aged 6-8 years (480/120 males/females) were included. In addition, three laboratory-bred, coccidian-free puppies aged 2-3 months were fed infected bovine muscles to locate the definitive host and analyze sporogony. 18S rRNA-specific gene primers were used for DNA amplification from esophageal muscles. These polymerase chain reaction (PCR) amplicons were subjected to restriction fragment length polymorphism (RFLP) and molecular sequence analysis. Infection was detected in 78.8% (473/600; 95% CI, 75.56-82.11%). Histopathological examination of esophageal muscles showed oval- to spherical-shaped cysts, 96.7 μm wide by 326.9 μm long; cysts in cardiac muscles were ovoid and smaller. Infected puppies began shedding sporocysts in feces 7 days post-inoculation and showed distorted organ architecture, severe cellular damage, and inflammatory lesions in liver, kidney, esophagus, and stomach. Three oocysts with different shapes and sizes were identified. Partial 18S rRNA gene sequences of isolated New Valley sarcocysts were identical to S. cruzi isolated from different areas, verifying their genetic relatedness. Our analysis suggests that S. cruzi is the most prevalent in slaughtered cattle in New Valley Governorate, Egypt.

THE CLASSICISM IN OF SACRED SPACE FORMATION

The classicism of the first decade of the 20th century was analyzed as a result of a negative assessment of the situation in the culture of the late 19th century. The opinions of such cultural theorists as Max Nordau or Richard Hamann, who represent extreme individualism and isolation of art from moral and social tasks, are manifestations of degeneration and decline, leading society to madness and collapse. The features characteristic of the culture of the end of the century (fin de siècle) are presented.
 The architecture of sacred structures built on the principle of classical architecture is considered. It is determined that the objects created by Jozsef Plečnik, Gunnar Asplund and Sigurd Lewerentz, which carried super-individual, inhuman forms of industrial classicism, could seem terrible, but in this case they were associated with the features of a single reliable support of the human community.
 A look at the mysterious nature of the connection of people in society is presented - close to the types of connections connecting religious communities, as well as the concept of architecture as a bridge between heaven and earth, which changed the approach to the building as an exclusively material object.
 The forms of individual sacred structures and the ways of organizing their interiors are analyzed, which made it possible to conclude that the ideas of classicism of the unity of the surrounding landscape, the tendency to use local materials, but also a more general, philosophical attitude, allowing us to consider architecture as an element that connects the forces of space with human life.
 The use of the metaphorical language of architecture based on visual human products and their iconographic distinctive features is considered. The need to take into account the relationship between certain forms and messages through which they are transmitted in the formation of sacred space is revealed.
 The tendencies of shaping the sacred space in the 20th century, the role of local traditions and natural materials, organic architecture are revealed.

Pre-synaptic DC bias controls the plasticity and dynamics of three-terminal neuromorphic electrolyte-gated organic transistors

The role of pre-synaptic DC bias is investigated in three-terminal organic neuromorphic architectures based on electrolyte-gated organic transistors—EGOTs. By means of pre-synaptic offset it is possible to finely control the number of discrete conductance states in short-term plasticity experiments, to obtain, at will, both depressive and facilitating response in the same neuromorphic device and to set the ratio between two subsequent pulses in paired-pulse experiments. The charge dynamics leading to these important features are discussed in relationship with macroscopic device figures of merit such as conductivity and transconductance, establishing a novel key enabling parameter in devising the operation of neuromorphic organic electronics.

Location of olfactory organs and architecture of gustatory organs in the poultry red mite, Dermanyssus gallinae (Acari: Dermanyssidae)

Dermanyssus gallinae is a hematophagous ectoparasitic mite in the poultry industry worldwide. Its current control mainly relies on spraying acaricides with many deficiencies. It's urgently needed to develop novel control approaches, such as pheromones. The pheromones are detected by chemoreceptors, which are not well investigated in D. gallinae. In the current study, Y-tube olfactometer bioassays revealed that extracts of feces of mites could attract D. gallinae, indicating aggregation pheromones existed in feces. After varnishing the tips of the foreleg tarsi, the female mites' response to feces extracts disappeared in Y-tube olfactometer bioassays. Still, their response to guanine (a non-volatile compound acting as a potential arrestment pheromone) remained in filter paper contact bioassays. These results suggested that the sensilla at tips of the foreleg tarsi had olfactory function, and the sensilla at the tips of the pedipalpal tarsi could have gustatory function and the gustatory organs were located in other body parts of female mites. Through scanning electron microscopy (SEM), it was found that adults and nymphs possess a cluster of 8 peg-like sensilla as well as 2 blunt, peg-like sensilla at the tip of each pedipalpal tarsus. Nevertheless, the larvae had only a cluster of 5 blunt peg-type sensilla. Each sensillum has a terminal pore or depression, suggesting it functions as gustatory organ. The present study confirmed the location and explored the architecture of gustatory organs of D. gallinae, which would facilitate the development of novel control method based on pheromones.

Acute toxicity study of hydroalcoholic extract of two Indian medicinal plants Alternanthera ficoidea and Ludwigia octovalvis

Background: The ethano-pharmacological survey on two Indian medicinal plants Alternanthera ficoidea and Ludwigia octovalvis revels that both plants are biologically potential and effective in the management of various diseases. Despite the both the plants as whole are used traditionally by the local people in Indian subcontinent but the toxicity studies on whole plant extract of both plant has not explored scientifically. Objective: The toxicity study was designed to explore the toxicity potential associated to hydroalcoholic extract of whole plant of Alternanthera ficoidea and Ludwigia octovalvis according OECD guidelines. Material and methods: Healthy Charles Foster albino female rats of 8-12 weeks’ old were divided in 3 groups. Group I, group II, and group III received vehicle (0.5% CMC, p.o., single dose), hydroalcoholic extract of whole plant of Alternanthera ficoidea (HAF) (Single dose of 2000 mg/kg, given orally), and hydroalcoholic extract of whole plant of Ludwigia octovalvis (HLO) (Single dose of 2000 mg/kg, given orally), respectively. After 14 days, all rats were anesthetized with chloroform and blood were collected by retro-orbital capillary puncture for the study of haematological and biochemical parameters. After blood collection, rats were sacrificed; organs were carefully collected, weighted and examined for changes. Results: The acute toxicity study on Charles foster female rats showed that there was no sign and symptoms of toxicity were seen in rats of all group. Haematological parameters, biochemical parameters and histopathological architectures of various organs of treated groups were found to be non-significant and normal in range when compared with normal control groups. Conclusion: The study found that no mortality occurred at a dosage of 2 g/kg of HAF and HLO, and it came to the conclusion that the IC50 of HAF and HLO was higher than that level.

Polyoxometalate-based metal–organic supramolecular architectures derived from two new pyrimidine-amide ligands as supercapacitors and multifunctional electrochemical sensors

Two polyoxometalate (POM)-based metal–organic supramolecular architectures based on two new pyrimidine-amide ligands were prepared. Both of them possess high specific capacitance, good sensitivity and low detection limit for Cr(vi) and Fe(iii) ions.

Antimalarial Efficacy and Safety of Senna occidentalis (L.) Link Root Extract in Plasmodium berghei-Infected BALB/c Mice.

Emergence of Plasmodium resistance to antimalarial drugs presents a major drawback in efforts to control malaria. To address this problem, there is an urgent and continuous need for the development of new and effective antimalarial agents. Senna occidentalis (L.) link extract has exhibited in vitro antiplasmodial activity in many pharmacological studies. To our knowledge, data on its in vivo antimalarial efficacy is still very limited. A recent study demonstrated that polar extracts from the plant roots inhibit Plasmodium berghei proliferation in a mouse model. This study further describes the efficacy and safety of a methanolic root extract of the plant as an antimalarial agent by demonstrating its effect on hematological, biochemical, and histological parameters of Plasmodium berghei-infected BALB/c mice. Rane's test, a curative approach, was used to evaluate the antimalarial efficacy of Senna occidentalis methanolic root extract in Plasmodium berghei-infected BALB/c mice. The effect of the extract on both hematological and biochemical parameters was evaluated using automated analyzers. Kidney, liver, lung, spleen, and brain tissues were harvested from euthanized mice and examined for changes in organ architecture. This study demonstrates that methanolic root extract of Senna occidentalis significantly inhibited Plasmodium berghei parasitemia in BALB/c mice (p < 0.01). Infected mice that were treated with the extract depicted a significantly low level of total leucocytes (p < 0.01), red blood cell distribution width (p < 0.01), and a significantly high hemoglobin concentration (p < 0.001) compared to the infected animals that were administered with the vehicle only. The infected animals that were treated with the extract exhibited a significantly low level of urea, creatinine, bilirubin, and alkaline phosphatase (p < 0.05), compared to the infected animals that were given the vehicle only. The level of sodium, potassium and chloride ions, lymphocytes, granulocytes, hematocrit (HCT), mean corpuscular volume (MCV), mean corpuscular hemoglobin (MCH), mean corpuscular hemoglobin concentration, total protein, albumin, aspartate aminotransferase (AST), alanine transaminase (ALT), alkaline phosphatase (ALP), total platelets, mean platelet volume (MPV), and platelet distribution width of the infected animals treated with the extract was not significantly different from those of the infected animals that were given the vehicle only (p > 0.05). The extract alleviated organ pathological changes in the infected mice. The extract did not induce any remarkable adverse effect on the growth, hematological, and biochemical parameters of uninfected animals (p > 0.05). In addition, administration of the extract did not alter the gross appearance and histological architecture of the organs, implying that the extract was well tolerated in mice. Senna occidentalis methanolic root extract exhibited good antimalarial activity against Plasmodium berghei and may be safe in mice.

Advances in Brain Metastasis Models

To obtain achievements in addressing the clinical challenges of brain metastasis, we need a clear understanding of its biological mechanisms. Brain metastasis research is challenged by many practical scientific barriers. Depending on the purpose of the study, experimental brain metastasis models in vivo can be used. It is now possible to re-create the architecture and physiology of human organs. Human organoids provide unique opportunities for the study of human disease and complement animal models. The translation of experimental findings to clinical application has several barriers in the development of treatment for brain metastasis. A variety of models have provided significant contributions to the knowledge of brain metastasis pathology and remain pivotal tools for examining novel therapeutic strategies.

CXCL12/CXCR4: An amazing challenge and opportunity in the fight against fibrosis.

Fibrosis is a pathological process caused by abnormal wound healing response, which often leads to excessive deposition of extracellular matrix, distortion of organ architecture, and loss of organ function. Aging is an important risk factor for the development of organ fibrosis. C-X-C receptor 4(CXCR4) is the predominant chemokine receptor on fibrocytes, C-X-C motif ligand 12 (CXCL12) is the only ligand of CXCR4. Accumulated evidence have confirmed that CXCL12/CXCR4 can be involved in multiple pathological mechanisms in fibrosis, such as inflammation, immunity, epithelial-mesenchymal transition, and angiogenesis. In addition, CXCL12/CXCR4 have also been shown to improve fibrosis levels in many organs including the heart, liver, lung and kidney; thus, they are promising targets for anti-fibrotic therapy. Notably, inhibitors of CXCL12 or CXCR4 also play an important role in various fibrosis-related diseases. In summary, this review systematically summarizes the role of CXCL12/CXCR4 in fibrosis, and this information is of great significance for understanding CXCL12/CXCR4. This will also contribute to the design of further studies related to CXCL12/CXCR4 and fibrosis, and shed light on potential therapies for fibrosis.

Dynamic covalent self-assembly and self-sorting processes in the formation of imine-based macrocycles and macrobicyclic cages.

Investigating the self-assembly and self-sorting behaviour of dynamic covalent organic architectures makes possible the parallel generation of multiple discrete products in a single one pot procedure. We here report the self-assembly of covalent organic macrocycles and macrobicyclic cages from dialdehyde and polyamine components via multiple [2 + 2] and [3 + 2] polyimine condensations. Furthermore, component self-sorting processes have been monitored within the dynamic covalent libraries formed by these macrocycles and macrobicyclic cages. The progressive assembly of the final structures involves intermediates which undergo component selection and self-correction to generate the final thermodynamic constituents. The homo-self-sorting observed seems to involve entropic factors, as the homoleptic species present a higher symmetry than the competing heteroleptic ones. This study not only emphasizes the importance of an adequate design of the components of complex self-sorting systems, but also verifies the conjecture that systems of higher complexity may generate simpler outputs through the operation of competitive self-sorting.

Cardiovascular Effects of Nigella Sativa on Isolated Rabbit Heart and its Impact on Cardiac Remodeling

Background: Black Cumin/Nigella sativa (NS) which belongs to the botanical family of Ranunculaceae commonly grows in Eastern Europe, the Middle East, and Western Asia. Its prolonged use can produce physiological changes with or without affecting the architecture of different organs like the heart (cardiac remodeling). The data for the cardiovascular benefits of black cumin are not well-established scientifically. Objectives: To determine the direct cardiovascular effects of Nigella Sativa extract on heart rate, cardiac contractility (apical force), ECG, and coronary flow in the normal heart with and without cardiac remodeling. Methods: This experimental study was conducted on forty-two (42) rabbits. These rabbits were divided into seven groups, each comprising six animals (Group I-VI without cardiac remodeling and Group VII with cardiac remodeling). NS was given to these groups in different doses i.e., Group I (NS=10ug), Group II (NS=30ug), Group III (NS=100ug), Group IV (NS=300ug), Group V (NS=3000ug), Group VI (NS=10000ug) and VII (NS=300ug). Radnoti's working heart system was used to determine the effects of NS on heart rate, cardiac contractility (apical Force), ECG, and coronary flow in a normal heart with and without cardiac remodeling. The results were analyzed using SPSS version 28. Results: Results of this study revealed negative chronotropic and positive inotropic effects without ECG changes in the normal heart and with ECG changes in the remodeled heart. Conclusions: Prolonged use of Nigella sativa can lead to disturbed ECG by affecting the conducting tissue.

Development and Optimization of Methotrexate Encapsulated Polymeric Nanocarrier by Ionic Gelation Method and its Evaluations

AbstractMethotrexate encapsulated nanocarriers (M‐CNCs) were synthesized via the ionic gelation method and using Taguchi design to optimize the formulation for; size, percent entrapment efficiency (%EE), and percent drug loading (%DL). The M‐CNCs are designed to improve therapeutic efficacy and minimize the side effect of Methotrexate. Various critical factors, such as pH, polymer to a cross‐linker ratio (v/v), and reaction time (h), are considered to investigate their influence on the selected responses during the optimization. The physicochemical properties and morphology of the optimized M‐CNCs were investigated by Dynamic light scattering, Fourier transforms infrared spectroscopy (FTIR), X‐ray diffraction, Thermogravimetric analysis, and High‐resolution transmission electron microscopy. Further, In‐vitro release, stability, pharmacokinetics, and toxicity were studied. The FTIR result revealed the cross‐linking between the drug and polymer. The nanocarrier was found to be stable with spherical morphology and crystalline nature. Optimized formulation shows, Zavg=155 nm, EE=87 %, and D=49 %. The In‐vitro drug release showed a controlled release profile of MTX up to 72 h. Pharmacokinetic evaluation of M‐CNCs in healthy Sprague Dawley rats showed an improvement in plasma drug concentration. Toxicity evaluation by the histology study revealed a healthy organ architecture and the absence of pathological lesions. Overall, the formulated M‐CNCs showed enhanced bioavailability with no toxicity.