Archaeological Sites Research Articles

Suggested mechanism of CCR5Δ32, CCR2-64I and SDF 1-3'A allele frequency change in Polish and Lithuanian gene pools from the perspective of passing time.

The study aimed to determine the frequency of the alleles associated with hereditary immune response in 16 historical populations and assess which evolutionary forces may have contributed to the observed frequency fluctuation. The analysed polymorphic sites are located in three genes - CCR5, CCR2 and SDF 1 (CXCL12). Protein products are involved in the innate immune response and are also involved in various types of infections, autoimmune diseases and tumours. The frequency of the alleles found in the DNA of the studied individuals was determined by the Sanger methodology and was compared with the data obtained for modern populations. To confirm the authenticity of the obtained results, mtDNA HVRI haplotypes of all the studied samples were obtained and compared with the genetic database of the laboratory personnel who came into contact with the studied material. Based on the variability of allele frequency, advanced biostatistical analysis was used to distinguish the effect of natural selection from genetic drift, i.e. the forces operating on the polymorphic sites studied. All procedures were performed according to the guidelines for working with ancient DNA to avoid contamination with modern DNA molecules. 681 samples from 39 archaeological sites in Poland and Lithuania dated to the 40th century BC and the 19th century were studied. The biostatistical analysis showed that the fluctuations in the frequency of CCR5Δ32 in the analysed time interval could be mainly the effect of genetic drift. Nevertheless, for CCR2-64I and SDF 1-3'A, the results confirm the suggestion of negative selection as the mechanism involved. Since all the polymorphic sites encode the elements of innate immune response that are indirectly associated with the process of an HPV infection and the development of cervical cancer, the human papillomavirus may be a good candidate for a selection coefficient affecting the frequency of CCR2-64I and SDF 1-3'A. However, for CCR5Δ32, selection was not detected despite its proven role in the molecular mechanism involved in the response to an HPV infection. The presented work seems to be the first in which the problem of the pattern of CCR5Δ32, CCR2-64I and SDF 1-3'A frequency fluctuations in a temporal perspective was discussed, proposing HPV as a factor influencing the occurrence of the CCR2 and SDF1 alleles.

From the Berelekh ‘mammoth graveyard’ to Berelekh geo-archaeological complex: Paleoenvironment, site formation processes, and human-mammoth relationships

The Berelekh ‘mammoth graveyard’ is located on the Berelekh River in the NE Yana-Indighirka Lowland at 70° 30′ N and 144° 02′ E in the East Siberian Arctic. First studied in 1970, it was originally viewed as an example of a natural accumulation of mammoth remains in a periglacial landscape, although the discovery of associated archaeological remains indicated a human presence. New field research has yielded more data on stratigraphy, site formation processes, paleoenvironments, and age, allowing reassessment of the relationship between the mass accumulation of mammoth remains and archaeological site, which together constitute the Berelekh geo-archaeological complex (BGAC). The accumulation of mammoth bone spans 13,700–11,800 14C yr B.P., with respective peaks at 12,400, 12,200, and 11,800 14C yr B.P. The peaks fall within the Bølling-Allerød warming, represented in the Berelekh pollen record. Based on the taphonomy and dating of the BGAC, humans contributed to the formation of the bone-bed and procurement of mammoth was related to production of mammoth ivory technology. The dating of the mammoth bone accumulation coincides with the age of the archaeological site, indicating recurring but not permanent human occupation of the area. Based on IntCal20 calibration, the archaeological component of the Berelekh geo-archaeological complex dates to ∼14,000–13,500 calBP.

Luminescence dating of adobe from monumental architecture in north coastal Peru

Luminescence dating was applied to adobe bricks from temples at several archaeological sites in coastal Peru. Resulting dates, ranging from BCE 100 to CE 920, are generally in agreement with associated radiocarbon assays, ca. 350–950 CE. Luminescence dates the last time bricks were exposed to sunlight. Because the bricks were likely made in molds, not all grains in the bricks were likely exposed to sufficient sun at time of manufacture. Use of single-grain dating is essential. Luminescence was measured on 180–212 µm potassium feldspar using infrared stimulated luminescence with correction for anomalous fading. A minimum age model was applied to isolate those grains most likely to have been fully exposed. Some dates were also derived for associated sediments.

Feeding the Philistine city. An isotopic investigation of animal resources at Ashkelon in the Iron I

The Iron I period (1200–1000 BCE) at the archaeological site of Ashkelon, Israel ushers in the beginnings of Philistine control of the city. Varied taxonomy in the animal bone remains in the context of domestic dwellings at Ashkelon reveals shifting patterns of consumption over time (Hesse and Fulton, 2020;Master and Aja, 2020). One hypothesis suggests that the use of cattle as traction/draught animals as part of an intensive agricultural system led to increased cattle bone remains in the Iron I, and these animals were likely pastured in fields directly related to the city (Hesse and Fulton, 2020). This hypothesis is tested by applying geographical and environmental interpretations to the isotopic composition of tooth enamel (carbon, oxygen and strontium) from domestic animals including cattle, donkeys, sheep and goats (caprines), and pigs. The hypothesis of draught cattle is not supported. While cattle were intensively managed, they were grazed in diverse locations across the landscape not centered around the city of Ashkelon. This specialized production strategy would have necessitated important cooperative social and economic relationships between urban consumers and inland farmers and herders who provided animals to the city.

Intracemetery relationships: Non-metric trait combination analysis of Maspalomas of Gran Canaria (Canary Islands, Spain).

Maspalomas is one of the most important archaeological sites in Gran Canaria, Canary Islands. The necropolis is one of the few funerary sites on the island where several the skeletons were found in anatomical position. The burials correspond to graves and cists dated between the 12th and 15th century CE. These graves and cists are clustered together in different formations across the necropolis, giving rise to a complex composition that denotes the existence of possible interpersonal relationships of the people buried there. A total of 135 calcanei and 118 tali were analysed to find non-metric traits and to test whether the clustered burials share a non-metric trait relationship. Trait combinations were formed using talus and calcaneus non-metric traits separately. The results of this study suggest that the individuals of Maspalomas showed a very high prevalence of lateral and medial talar facets, attributed to prolonged squatting position and/or walking on uneven ground. The calcaneal facet pattern (that may be aetiologically genetic) is more closely related to that observed among North-Africans or Indians than to Western Europeans. Calcaneal facet type Ib, and other genetically-determined traits, such as the extra facet extension of Posterior Facet, or the medial root of the inferior extensor retinaculum trait, either as single traits or as the combination of both traits, were significantly associated with individuals buried in different geographical areas of the necropolis defined by differences in burial structures, a finding that may suggest that genetically-linked individuals were buried in a separate area of the necropolis. The use of trait combination analysis in this study shows that the method can be applied to identify relationships among genetically or professionally related individuals that were subjected to a different burial procedure by their contemporaries.

Structural Assessment of Late Neolithic Sa Covaccada Dolmen, Sardinia, Italy

ABSTRACT The application of structural engineering tools to archaeological sites is unusual, while architects, archaeologists, and restorers are more commonly involved in these scenarios. Nevertheless, it can provide valuable indications for conservation and repair. This paper presents the structural assessment of the late Neolithic Sa Covaccada Dolmen, in Sardinia, Italy, regarded as a unicum in the Region and one of the most important megalithic monuments in the Mediterranean area. Due to severe material deterioration and extensive crack pattern, structural stability is a primary concern, yet it has not been assessed to date. To address this, both analytical rigid body analyses and 2D and 3D discrete element simulations were performed, contributing to an as challenging as fascinating multidisciplinary restoration project.

Color-based discrimination of color hues in rock paintings through Gaussian mixture models: a case study from Chomache site (Chile)

The article explores advanced image processing techniques for pigment discrimination in rock art paintings, emphasizing color separation using RGB (red, green, blue) and LHCUv (Luminance, Hue, Chroma) imagery. It highlights the use of dimensionality reduction methods such as Principal Components Analisys PCA and Independent Component Analysis (ICA), with a focus on Gaussian Mixture Models (GMM) for probabilistic classification of image elements. This approach, applied to the Chomache archaeological site on the northernmost coast of the Atacama Desert in Chile, reveals previously undetected motifs and details, offering a nuanced perspective in rock art documentation and analysis. This proposal reinforces the value of rock art panel not only as a finished product but as a process.

The late Early Pleistocene site of Fuente Nueva-3 (Guadix-Baza Depression, SE Spain): a hyena latrine developed on a quicksand trap for megaherbivores?

AbstractThe late Early Pleistocene archaeological site of Fuente Nueva-3 (FN3), which lies in the Guadix-Baza Depression (SE Spain) and is dated to ~ 1.4 Ma, contributes some of the oldest evidence of hominin presence in Western Europe, including a huge tool assemblage of Oldowan tradition, manuports (i.e., unmodified stones used as percussion tools) and abundant fossils of large mammals, some of which preserve anthropogenic marks related to defleshing, butchering and marrow processing. In addition, there are bones with tooth marks produced by scavenging carnivores. The fertile layers of the FN3 section have been grouped in a lower archaeological level (LAL) and an upper archaeological level (UAL). Both levels preserve abundant skeletal remains and lithic tools. However, the LAL shows a high density of manuports, which suggests that hominin activity was more intense at this level, while the UAL preserves many remains of megaherbivores, particularly proboscideans (Mammuthus meridionalis), and almost all coprolites unearthed from the site, which points to a greater involvement of the giant hyenas (Pachycrocuta brevirostris). In this paper, we (i) test for statistical differences in the composition of the faunal assemblages preserved in the UAL and LAL; and (ii) analyze particle size in the fertile layers of both archaeological levels. Our results show that megaherbivores are comparatively overrepresented in the UAL, specially by young elephants, while other medium-to-large and large-sized ungulates, particularly equids, are more abundant in the LAL, showing a predominance of adult individuals. Concerning the sedimentology, layers 2–3 of the LAL show a predominance of silts and clays, with sands representing a minor fraction. In contrast, layer 5 of the UAL is composed of two-thirds of fine and very fine sands, with the remaining third consisting of silts and clays. These data and the paleoenvironmental reconstruction of layer 5 suggest that it may be interpreted as a paleo-quicksand in which megaherbivores were trapped due to the elevated weight per unit area supported by their feet, and their half-sunken carcasses attracted the scavengers, particularly the hyenas, which fed on these carcasses and defecated in their surroundings.

A Semi-Automatic-Based Approach to the Extraction of Underwater Archaeological Features from Ultra-High-Resolution Bathymetric Data: The Case of the Submerged Baia Archaeological Park

A Semi-Automatic-Based Approach to the Extraction of Underwater Archaeological Features from Ultra-High-Resolution Bathymetric Data: The Case of the Submerged Baia Archaeological Park

Precision Forestry Versus Non-Precision Archaeology—Integrating Forest Management and Archaeological Site Protection

This study examines the precision of spatial data required for archaeological sites in Finland from a forest management perspective, identifies archaeological sites at risk due to forestry activities, and investigates potential collaboration between archaeology and forestry to enhance the preservation and understanding of these sites. Due to outdated methods and the lack of systematic field surveys, challenges in achieving accurate spatial data for archaeological sites are highlighted through a literature review, case studies, and fieldwork. Our findings indicate that a significant portion of sites lack boundary delineation, exposing them to the risk of destruction. Moreover, the study reveals limited collaboration between the archaeology and forestry sectors, despite mutual interests in site preservation. We advocate for improved spatial data accuracy using modern technological methods like precision GPS and enhanced cooperation between archaeologists and forestry professionals. This approach aims to protect Finland’s archaeological heritage while promoting responsible forestry practices. Notably, fieldwork enabled the precise determination of the location of Finland’s first separate plague cemetery, underscoring the value of direct field verification in enriching historical and archaeological understanding.

Rice's trajectory from wild to domesticated in East Asia.

Rice (Oryza sativa) serves as a staple food for more than one-third of the global population. However, its journey from a wild gathered food to domestication remains enigmatic, sparking ongoing debates in the biological and anthropological fields. Here, we present evidence of rice phytoliths sampled from two archaeological sites in China, Shangshan and Hehuashan, near the lower reaches of the Yangtze River. We demonstrate the growth of wild rice at least 100,000 years before present, its initial exploitation as a gathered resource at about 24,000 years before present, its predomestication cultivation at about 13,000 years before present, and eventually its domestication at about 11,000 years before present. These developmental stages illuminate a protracted process of rice domestication in East Asia and extend the continuous records of cereal evolution beyond the Fertile Crescent.

Past and Future Impacts of the Relative Sea Level Rise on the Seafront of Ancient Delos (Cyclades, Greece) and Flooding Scenarios by 2150

Sea level rise due to global warming is a continuing and, disappointingly, accelerating process which has already affected and will further impact coastal lowlands and the social and economic activities in these areas. Delos Island, situated in the middle of the Cyclades in the Aegean Sea, was considered the most sacred of all islands in ancient Greek culture and was a trading hub for the entire eastern Mediterranean. Uninhabited since the 7th century AD, and consistently the focus of research and touristic attention, the island is designated as an archaeological site and inscribed on the UNESCO World Heritage List. Previous studies on the relative sea level (rsl) changes suggest a steadily rising rsl during the last 6300 years, starting from a sea level of −4.80 ± 0.20 m in the Late Neolithic. The seafront of the ancient city of Delos is subject to the effects of rsl rise, which have caused significant coastline retreat and exposure to the northerly winds and waves, whereas parts of the coastal lowland, where the remains of the ancient city lie, are inundated, forming extended wetlands. The future impacts of rsl rise on the seafront of ancient Delos are illustrated on very-high-resolution digital surface models, evaluating both the flooding risk under different climatic projections, as provided by the IPCC AR6 report, and the ongoing land subsidence, as recorded by GNSS data. An rsl rise ranging from 87 cm (SSP1-2.6 scenario) to 148 cm (SSP5-8.5 scenario) is anticipated by 2150, requiring both resilience strategies and adaptation solutions as well as mitigation policies to cope with the effects of climate change.

Multiple luminescence dating on heated materials at the nanzuo archaeological site, central Chinese Loess Plateau

Nanzuo is a key Neolithic archaeological site in the central Chinese Loess Plateau (CCLP). The site is important for understanding the origin and development of the Chinese civilization, yet, it lacks a robust chronological framework. The current work, for the first time, dates two different types of heated materials (pottery and burnt soil) from a profile near the main excavation of the Nanzuo archaeological site using various luminescence dating techniques. These techniques included single grain (SG) quartz OSL and K-feldspar post-infrared infrared-stimulated (pIRIR) dating for burnt soil samples and pottery, and pIRIR dating for pottery slices using the rock surface luminescence technique (RSL). The reliability of luminescence dating was investigated by comparing SG quartz, K-feldspar and pottery slice ages with radiocarbon (14C) ages obtained from the same cultural layer. The results show that the SG quartz OSL and K-feldspar pIRIR225 age estimates of the two coarse potteries are consistent with the 14C ages, however, the age estimates of the pottery slice (NZ22-CPS150) are significantly younger, which is primarily explained by underestimation in equivalent doses (De). Moreover, it is noted that while there is a considerable variation in the De of coarse pottery slices with depth, no significant correlation is observed between K- content and De. Heterogeneous K content was revealed by μ-XRF scanning on coarse pottery slices. Conversely, fine pottery displayed homogeneous K-content distribution and age estimates consistent with the 14C ages. Thus fine pottery slices are suggested to be more suitable for pIRIR dating relative to coarse pottery slices. The underestimation of De values in mixed minerals is observed as the primary reason for underestimated slice ages compared to SG K-feldspar, with grain size of K-feldspar and K content variation playing only a minor role. Therefore, by integrating the 14C ages with the luminescence ages of the pottery, it is inferred that human occupation of the Nanzuo site occurred around 5100 to 4500 years ago, which aligns with the 14C ages obtained from the main section of the Nanzuo site. The current work further concludes that the single-grain luminescence dating method is reliable for pottery. The study recommends to prioritize materials characterized by relatively uniform potassium (K) content for pIRIR dating using pottery slices.

Sturgeons in materials from archaeological sites of Ukraine: A review

AbstractThe article presents a critical review of all published data on findings of the skeletal elements of sturgeons (Acipenseridae) during archaeological excavations conducted in the territory of Ukraine. Sturgeon remains are present in materials from 41 archaeological sites, the age of which covers the stage from the Neolithic (4 kyr bc) up to the Modern Period (17th century ad). Five species (Acipenser gueldenstaedtii, Acipenser nudiventris, Acipenser ruthenus, Acipenser stellatus, and Huso huso) are recognized. Of them, the Russian sturgeon, the sterlet, and the stellate sturgeon are the most abundant, while beluga is somewhat less numerous, and the fringebarbel sturgeon is represented by the smallest number of remains. The presence of the latter species (and the absence of the European sturgeon Acipenser sturio) in the historical past of Ukraine is discussed. We also highlight the importance of original and published data accessibility in archaeozoological research and discuss the issues of species identification, body length, and weight reconstruction of sturgeons based on skeletal elements.

Archaeological Sites of Irmen Culture in the Tomsk Ob Region

Archaeological Sites of Irmen Culture in the Tomsk Ob Region

BRAIN - Holocene archaeo-data for assessing plant-cultural diversity in Italy and other Mediterranean regions

In the field of botany applied to archaeological and palaeoecological studies, the multi- and inter-disciplinary nature of this research produces a lack of data sharing and scattered articles in the specialty literature or in national and international journals. The vast production of archaeobotany and palynology data makes it necessary to develop a tool for the availability, accessibility, and dissemination of existing research. Many databases exist on palaeoecology, archaeobotany or pollen data. There are no collections focused on archaeological sites and human-induced environments and centred on Southern Europe and the Mediterranean. BRAIN - Botanical Records of Archaeobotany Italian Network is the first database listing sites from which all types of plant records are available in Italy and nearby Mediterranean regions. BRAIN represents the largest integrated collection of archaeo/palaeo-botanical data and a range of descriptive information that makes data recovery FAIR ready. This unique network hosts data on the availability of anthropogenic pollen, palynomorphs and plant macroremains in the same database, and experts of different research fields may contribute to it.

A macroscopic assessment of porosity and new bone formation on the inferior pars basilaris: Normal growth or an indicator of scurvy?

ObjectivesThis research aims to determine the aetiology of porosity and subperiosteal new bone formation on the inferior surface of the pars basilaris. MaterialsA total of 199 non-adult individuals aged 36 weeks gestation to 3.5 years, from a total of 12 archaeological sites throughout the UK, including Iron Age (n=43), Roman (n=12), and post-medieval (n=145) sites, with a preserved pars basilaris. MethodsThe pars basilaris was divided into six segments, with porosity (micro and macro) and subperiosteal new bone formation recorded on the inferior surface in scorbutic and non-scorbutic individuals. Scurvy was diagnosed using criteria from the palaeopathological literature that was developed using a biological approach. ResultsThere was no statistically significant difference in microporosity between scorbutic and non-scorbutic individuals in four out of the six segments analysed. There was a significant negative correlation between age and microporosity in non-scorbutic and scorbutic individuals. A significant difference in subperiosteal new bone formation was observed between scorbutic and non-scorbutic individuals. ConclusionsMicroporosity on the inferior pars basilaris should not be considered among the suite of lesions included in the macroscopic assessment of scurvy in non-adult skeletal remains (less than 3.5 years). SignificanceThis study highlights the risk of over diagnosing scurvy in past populations. LimitationsIt is difficult to distinguish between physiological (normal) and pathological (abnormal) bone changes in the skeleton of individuals less than one year of age. Suggestions for further researchFuture research should focus on the analysis of individuals over 3.5 years of age.

K. A. Zheleznyakov’s Study of the Archaeological Sites of Heishui Mohe in Northern Manchuria on the Ashihe River in the 1930s – 1940s

K. A. Zheleznyakov’s Study of the Archaeological Sites of Heishui Mohe in Northern Manchuria on the Ashihe River in the 1930s – 1940s

Past human decision-making based on stone tool performance: Experiments to test the influence of raw material variability and edge angle design on tool function

One of the main interests in the interpretation of the archaeological record and its variability within and through time and space is the production and use of past human stone tool technologies. Tool design and function are inevitably intertwined and strongly related to tool use. Understanding tool design provides information about early human technological adaptations and reflects human behaviour in the sense of conscious or unconscious decision-making. Nevertheless, the reason for major changes (including novelties, innovations, and loss) in past human stone tool technology is still poorly understood. A comprehensive approach focusing on tool function (What was the tool meant for?) and use (What was the tool used for?) can help to overcome this gap. While tool function (including performance) can be investigated experimentally, tool use can be addressed with use-wear analyses. These questions can be best investigated on technological systems showing little tool variability but strong evidence of maintenance and long-term use, such as Middle Palaeolithic industries.The Late Middle Palaeolithic record of Central and Eastern Europe is marked by the emergence of an asymmetric tool-type called Keilmesser (bifacial backed knives). Due to their sophisticated morphology, Keilmesser as a case study offer the potential to address aspects of raw material selection, tool production, maintenance, and reworking.This paper presents the results of an experiment designed to study the tool performance of Keilmesser from three archaeological sites, namely Balver Höhle, the Upper site of Buhlen and Grotte de Ramioul by testing raw material, edge angle and movement as independent variables. A highly controlled, sequential experiment was conducted using a mechanical device performing unidirectional cutting and carving movements on hard contact material. Results demonstrate the possibility to perform the mentioned task with 35° and 45° edge angles, maintaining function, albeit at differing levels of efficiency. The data has a direct impact on the interpretation of the archaeological assemblages regarding aspects such as stone tool morphology and resharpening. At the same time, the study highlights the importance of raw material analysis to understand the variability in the archaeological record and the implications on past human decision-making strategies.

A comparative study of commercially available, minimally invasive, sampling methods on Early Neolithic humeri analysed via palaeoproteomics

Due to methodological advances in the archaeological sciences, an increasing number of archaeological specimens undergo destructive sampling. However, the preservation of cultural heritage is a primary concern. This leads to a dilemma between accessing sample material and obtaining sufficient information for a meaningful analytical outcome. Ideally, sampling a specimen would preserve the object for further macro, micro, and molecular analyses. For palaeoproteomics, a number of minimally invasive sampling approaches have been proposed, representing different benefits and limitations. There have been studies comparing a selection of these protocols, however, these have focused on specimens from a homogenous preservation environment using Zooarchaeology by Mass Spectrometry (ZooMS). Here we expand on earlier work by extending the comparison to specimens from two highly different preservation environments through both ZooMS and liquid-chromatography tandem mass spectrometry (LC-MS/MS). We compare five sampling approaches and seven extraction protocols in total, on 10 Bos sp. humeri from the Early Neolithic site of La Draga, Spain, utilising MALDI-ToF MS and LC-MS/MS to generate proteomic output, while assessing protocol invasiveness using microscopy and 3D imaging. Five humeri originate from Sector A, which is mostly related to dry, terrestrial preservation conditions, while the other five humeri stem from Sector B, which is characterised by its phreatic/aquatic preservation conditions. We show that there is a significant difference in protein recovery and taxonomic specificity between the sampling techniques applied, as well as between burial conditions. Additionally, various surface modifications were observed depending on the specific sampling technique applied. It is therefore essential to assess protein preservation for each sedimentological context within an archaeological site before performing extensive sampling, as protein preservation can be highly inter- and intra-site-specific.