In the Neolithic pile-dwelling settlements of southwestern Germany, bark played a prominent role in the production of technical textiles. So far, the inner bark (phloem) of the lime tree (genus Tilia) could be detected most frequently. Microscopic examination of anatomical features can determine the taxon, requiring manipulation of samples and archaeological objects. In this study, micro-computed tomography (µCT) was reviewed as a method for determining the woody taxon and obtaining additional information from the inner bark. To this end, modern bark samples from different tree organs of lime were first analysed using both µCT and transmitted light microscopy. Both methods were able to detect all characteristic anatomical features in the phloem and identify the genus. With analysis based on µCT data, further anatomical information can be obtained. For example, the shape of the phloem rays in the bast strips can provide information on the position within the bark and on the original organ diameter. These results obtained on modern material were verified on four samples from archaeological objects. Based on µCT, all samples could be clearly identified as lime and in two cases conclusions could also be drawn about the raw material. This approach could lead to new results and interpretations in archaeological sciences.