Soil erosion is a most severe environmental problem in humid sub-tropical hilly state Tripura. The present study is carried out on Muhuri river basin of Tripura state, North east India having an area of 614.54 Sq.km. In this paper, Universal Soil Loss Equation (USLE) model, with Geographic Information System (GIS) and Remote Sensing (RS) have been used to quantify the soil loss in the Muhuri river basin. Five essential parameters such as Runoff-rainfall erosivity factor (R), soil erodibility Factor (K), slope length and steepness (LS), cropping management factor (C), and support practice factor (P) have been used to estimate soil loss amount in the study area. All of these layers have been prepared in GIS and RS platform (Mainly Arc GIS 10.1) using various data sources and data preparation methods. In these study DEM and LISS satellite data have been used. The daily rainfall data (2001-2010) of 6 rain gauge stations have been used to predict the R factor. Soil erodibility (K) factor in Basin area ranged from 0.15 to 0.36. The spatial distribution map of soil loss of Muhuri river basin has been generated and classified into six categories according to intensity level of soil loss. The average annual predicted soil loss ranges between 0 to and 650 t/ha/y. Low soil loss areas (<25 t/ha/y) have been recorded under very densely forested areas and intensely plantation (mainly Rubber plantation) area. The high rate (>70 t/ha/y) of soil erosion was found along the main course of Muhuri River.