Aqueous Silver Nitrate Solution Research Articles

PHYTOSYNTHESIS AND CHARACTERIZATION OF SILVER NANOPARTICLES FROM RUELLIA TUBEROSA (L.): EFFECT OF PHYSICOCHEMICAL PARAMETERS

Objective: The current study focused on synthesizing silver nanoparticles (AgNPs) using Ruellia tuberosa aqueous tuber extract (RTTE) and silver nitrate (AgNO3) solution. Methods: AgNPs were synthesized using an aqueous tuber extract of the medicinal herb R. tuberosa (L.). The existence of significant phytoconstituents involved in synthesizing the AgNPs was determined using the gas chromatography–mass spectrometry (GC–MS) study. We evaluated the physical and chemical parameters such as the effect of time, temperature, metal ion concentration, crude aqueous tuber extract concentration, and pH in the synthesis of nanoparticles. The AgNPs were characterized using ultraviolet (UV)–Vis spectroscopy, field emission scanning electron microscopy (FESEM), energy-dispersive X-ray spectroscopy (EDX), high-resolution transmission electron microscope (HRTEM), selected area electron diffraction (SAED), X-ray diffraction (XRD), and Fourier transform infrared (FTIR) techniques. Results: R. tuberosa tuber extract was rich in various phytochemical constituents which were identified by GC–MS. For biosynthesis, the optimal values were 1 mM AgNO3concentration, 0.1 mL of aqueous tuber extract, and a 40 min incubation temperature of 70°C. The existence of a characteristic surface plasmon resonance (SPR) peak at 421 nm indicated the biosynthesis of AgNPs using UV–Vis spectroscopy. At higher temperatures and alkaline pH, the development of AgNPs increased overtime and remained stable up to 4 weeks. FESEM, EDX, HRTEM, SAED, and XRD analysis revealed that most AgNPs were spherical, with an average size distribution of 34.9 nm and a crystalline phase, face-centered cubic lattice. Infrared (FTIR) spectroscopic analysis revealed that hydroxyl and amino functional groups were involved in the biosynthesis and stabilization of AgNPs. Conclusion: The synthesis of AgNPs from R. tuberosa aqueous tuber extract was a cost-effective process and environmental friendly.

The Ag+ Reduction Process in a Plasma Electrochemical System Tuned by the pH Value

The interactions between discharge plasmas and an aqueous solutions can enable the production of reactive species and charge transfer at the plasma-liquid interface, forming the plasma electrochemical system (PES). The PES are promising for diverse applications, such as nanomaterials synthesis, due to the activation of the solution chemistry by the plasma. In this paper, we investigate the influence of the solution’s pH value on the formation of silver nanoparticles (AgNPs) in a direct current (DC) PES. Dual argon DC plasmas are generated in an H-type electrochemical cell containing an aqueous solution of silver nitrate with pH values in the range of 1.99–10.71. By this design, the solution acts as a cathode at one end of the H-type cell, and as an anode at the other end. The results show that the AgNPs are formed at the anode except for the solution with the pH value of 1.99. However, at the cathode, the AgNPs only appear in the solution with the pH value of 10.71. We find that the solvated electrons and hydrogen peroxide produced by the plasma-liquid interactions are responsible for the Ag+ reduction at the solution anode and the solution cathode, respectively.

Morphological and Optical Properties of Silicon Nanostructure, Obtained by One Step Ag-assisted Chemical Etching

Silver-assisted one-step chemical etching (AACE) is a low-cost, straightforward method for producing silicon nanostructures to improve their light absorption; it includes the etching of the wafers in aqueous hydrofluoric acid (HF), silver nitrate (AgNO3) and nitric acid (HNO3) solution. Influence of various parameters, such as AgNO3 concentration (0.47, 0.58, and 1.17 mM), HF concentration (0.67, 0.9, and 1.129 M), HNO3 concentration (0.12, 0.24, and 0.37 M), etching temperature (40, 50, and 60oC), and etching time (4, 5, and 6 min), on the Morphological and optical properties of silicon wafers were investigated. The results show that these parameters have a main role in determining the nanostructure size. The reflection measurements show that the minimum reflectance with 11% achieved with 0.58 mM AgNO3, 1.129 M HF, and 0.12M HNO3 recipe. Field Emission Scanning Electron Microscopy (FESEM) appears that the morphology of the manufactured silicon is semi-spherical nanostructures with the formation of the porous surface on the surface of the wafers.

Decoration of Ag Nanoparticle on ZnO Nanowire by Intense Pulsed Light and Enhanced UV Photodetector

Zinc oxide (ZnO) nanowires (NWs) are wide-bandgap semiconductors that absorb ultraviolet (UV) radiation. Various post-treatment processes have been studied to improve the optical properties of the as-grown ZnO NWs. Among them, Ag nanoparticles (NPs) effectively improved the optical properties on the surface of the ZnO NWs. In this study, ZnO NWs were synthesized via the hydrothermal synthesis method. ZnO NWs were decorated with Ag NPs on the surface of the ZnO NWs in a silver nitrate (AgNO3) aqueous solution by intense pulsed light (IPL) irradiation. Ag NPs were successfully decorated under the following conditions: aqueous AgNO3 solution of 100 nM, an energy of 1 J/cm2, and an exposure time of 8 ms. The responsivity and sensitivity of the ZnO NW UV photodetectors increased by 7.43 and 3.37 times, respectively. The IPL process makes it possible to decorate Ag NPs in a simple manner within an extremely short time.

Biogenic Synthesis of Silver Nanoparticles using Aspergillus Aureoles (Endophyte) and Demonstration of their Anti- microbial Activity

The nanoparticles have been recognized to play vital coating roles in electronics, energy contact actions, biology, and medicine due to their unique properties for two decades. In the present study, silver nanoparticles were synthesized using an endophytic fungus, Aspergillus aureoles, and subjected to antimicrobial activity. The treatment of an aqueous solution of silver nitrate (3 mM) with cell-free filtrate of Aspergillus aureoles resulted in the production of stable and crystalline silver nanoparticles. Silver nanoparticles were characterized using different studies, such as Ultraviolet-Visible Spectroscopy, High-Resolution Transmission Electron Microscopy, X-ray Diffraction Spectroscopy, Fourier Transform Infrared Spectroscopy, and Dynamic Light Scattering. The antimicrobial activity was carried out by the well diffusion method. The nanoparticles were in a spherical shape with an average particle size of 12-15 nm as observed in a High-Resolution Transmission Electron Microscope. The crystalline nature and size of nanoparticles were confirmed by the X-ray Diffraction Spectroscopy and Selected Area Electron Diffraction, respectively. The Fourier Transform Infrared Spectroscopic study revealed the involvement of various functional groups of Aspergillus aureoles extract in stabilizing the nanoparticles. These nanoparticles exhibited potent antimicrobial activity against both gram-positive and negative bacteria, which was comparable to the standard antibiotic. Thus, the study established the possible use of green silver nanoparticles in controlling microbial infections and developing a novel broad-spectrum antimicrobial agent/s.

Immobilization of Silver Nanoparticles on the Surface of Cellulose Nanofibers Using High-Pressure Wet-Type Jet Mill

Composite material immobilized silver nanoparticles (NPs) on the surface of cellulose nanofibers (CNF) was prepared using a high-pressure wet-type jet mill. A mixture both containing an aqueous silver nitrate solution and a CNF suspension was prepared as a raw starting material. The mixture was pulverized with the high-pressure wet-type jet mill at a pressure of 100 or 200 MPa. An X-ray diffraction pattern of the obtained sample revealed the presence of not only cellulose type I crystallites but also silver metal crystallites. According to observation by field-emission scanning electron microscopy, it was found that many silver NPs were immobilized on the surface of CNF. Note that almost all the silver NPs were well dispersed on the surface of CNF. It was cleared that the silver NPs had a spherical in shape with an average particle size of about 3 nm by the transmission electron microscope observation. The average size of the silver NPs slightly increased with the number of jet milling cycles, however, the change in the discharge pressure of the high-pressure wet-type jet mill did not affect the NPs size. The silver content in the composite materials increased with increasing both the number of jet milling cycles and the discharge pressure. The silver NPs were deposited by using the thermal energy released in the jet milling process, and their grain growth was then inhibited because the suspension was cooled immediately through the cooling tube. Therefore, it was assumed that silver NPs with a narrow size distribution could be immobilized on the surface of CNF.

A Novel Tetranuclear Silver Compound with bis(3,5-dimethylpyrazol-1-yl)acetate: a Simple Synthesis Yielding Complex Crystal Structure

A novel tetranuclear silver coordination compound with the formula [Ag4(bdmpza)4]×10H2O (bdmpza = bis(3,5-dimethylpyrazol-1-yl)acetate) was synthesized by a reaction between an aqueous solution of silver nitrate and an aqueous solution prepared by bis(3,5-dimethylpyrazol-1-yl)acetic acid and potassium hydroxide (1:1 molar ratio). The obtained compound was characterized by elemental analysis, coupled thermogravimetric-mass spectrometry analysis, vibrational IR spectroscopy, and its crystal structure was determined by single-crystal X-ray diffraction method. Furthermore, the obtained crystal structure was additionally evaluated by Hirshfeld surface analysis.

Green Synthesis, Antioxidant and Antimicrobial Activity of Silver Nanoparticles Using Gnetum africanum Extracts

The synthesis and application of nanoparticles is an important area of research that is gaining attention recently. In this recent project, we report the synthesis of silver nanoparticles, AgNP using aqueous solution of silver nitrate and Gnetum africanum leaf extract (reducing agent). The synthesis of AgNP was achieved by mixing aqueous solution of silver nitrate (70ml, 15.75mM) with a solution of Gnetum africanum leaf extract 100 ml) in a reaction flask and allowed to stand for 24 hours in a dark cupboard. A color change from light brown to yellowish brown was observed which indicated that synthesis of silver nanoparticles took place. The presence of AgNP was ascertained using UV-vis spectra analysis and absorption at 442 nm showed the presence of AgNP. The antioxidant assay of both the synthesized AgNP and the leaf extract was determined using DPPH. Antimicrobial activity was conducted using three different organisms which were Staphylococcus aureus, Escherichia coli and Pseudomonas respectively. The antioxidant results using DPPH scavenging ability of AgNp showed that at concentrations of 2mg/ml,1mg/ml and 0.1mg/ml, the percentage inhibition of DPPH by AgNp was 61.69, 53.06 and 38.31 respectively and that of Gnetum africanum leaf extract was 81.32, 78.49, and 58.29 respectively at the same concentrations using Ascorbic acid as a standard. The antimicrobial activity of both the synthesized AgNps and Gnetum Africanum Leaf extract using one gram positive bacteria (Staphylococcus aureus) and two gram negative bacteria (Escherichia coli and Pseudomonas) revealed that the synthesized AgNps showed lesser activity than Gnetumafricanum leaf extract for both the gram positive bacteria (Staphylococcus aureus) and gram negative bacteria (Pseudomonas) and (Escherichia coli). From the above findings, it can be observed that Gnetum Africanum Leaf extract reduced Ag+ to Ag0 and also both the synthesized AgNps and the Gnetum Africanum Leaf extract showed reasonable antioxidant activity against DPPH and antimicrobial activity against the tested microorganisms. This implied that both samples have medicinal values.

Green synthesis of silver nanoparticles using aqueous extract of Acacia cyanophylla and its antibacterial activity

AimsThe aim of this research is to conduct green synthesis of silver nanoparticles in an eco-friendly, economical and more effective approach using Acacia cyanophylla plant extract as well as to study the effects of the preparation conditions on the size of synthesized nanoparticles and its antibacterial activity. MethodologyIn this study, silver nanoparticles have been synthesized by reduction method using aqueous silver nitrate solution and aqueous extract of Acacia cyanophylla. Then, their characterization has been studied by several methods, such as visual inspection, UV–Vis spectroscopy, dynamic light scattering and scanning electron microscope. In addition, the effects of (silver nitrate: extract) ratio, type extract, temperature and reaction time have been studied on the size of prepared silver nanoparticles. Furthermore, the antibacterial effect of these nanoparticles was studied on Escherichia coli using micro-dilution method and determination the Minimum Inhibitory Concentration (MIC). ResultsThe results showed that the silver nanoparticles prepared using Acacia cyanophylla extract have reported visible yellowish brown color formation and the absorption peak at 460 nm indicated the biosynthesis of silver nanoparticles. Moreover, they have average diameter (88.11) nm and the polydispersity index (PdI) was suitable. The optimal conditions for synthesis silver nanoparticles were using aqueous extract in 9:1 ratio (silver nitrate: extract) at 35 °C for 48 h. These silver nanoparticles were stable in the in the fridge at 5 °C for a maximum period of 15 days. On the other hand, the antibacterial tests showed that these nanoparticles have high antibacterial activity where the MIC value ranged between (3.125–12.5) μg/ml on E. coli isolates. ConclusionWe conclude that Acacia cyanophylla extract is considered effective as a reducing agent for the preparation of stable silver nanoparticles in certain conditions and this silver nanoparticle has a high antibacterial activity.

Use of Aerial Roots of Ficus benghalensis for Green Synthesis of Silver Nanoparticles with Enhanced Antibacterial Activity

Basically, nanosubstances are developed by a variety of chemical methods which are not environmentally providential.
 Aim: The present research work deals with the synthesis of silver nanoparticles using the aerial root of Ficus benghalensis extract. The absolute reduction of silver ions was observed after 48 h of reaction when extact combine with aqueous solution of Silver nitrate. The visual colour changes were observed during the reduction of silver ion into the silver nanoparticles in the reaction mixture allows producing dark brown colour. The formed silver nanoparticles was purified by high speed centrifugation, collected and stored for further characterization.
 Methodology: The formation of silver nanoparticles was confirmed by UV-Visible spectroscopy, and characterised by X-Ray Diffraction (XRD) pattern, FTIR, High Resolution Transmission Electron Microscopy (HRTEM), Zeta potentiometry, ICP-AES.
 Results: The results showed that UV peak at 437.5 nm, the silver content estimation by ICP-AES was found to be 413.06 µg/mL and images were recorded by using High resolution TEM. Synthesized AgNPs were found to be effective against micro-organisms responsible for bacterial infections like Staphylococcus aureus, Pseudomonas aeruginosa, E. coli and Methicillin resistant Staphylococcus Aureus (MRSA). Further In-vitro cytocompatibility studies showed lack of toxicity at even higher concentration. 
 Conclusion: Still, these Silver Nanoparticles are cytotoxic in nature and could serve as a good green method for synthesis of silver nanoparticle by using plant extract.

Influence of Surfactant Additives on Photochemical Synthesized Silver Nanoparticles using UV Pulsed Laser Irradiations in Aqueous Silver Nitrate Solution

The effect of different additives on the AgNPs formation process was explored in this study. AgNPs were synthesized in an aqueous solution of silver nitrate-containing surfactants by photoreduction of silver ions. The concentration dependency of AgNPs formation suggested that stability was induced by the equilibrium of AgNPs adsorbed by surfactants with higher carbon chain molecules such as SDS and AOT. These results open up a new window both for structural control and the development process. It also indicated that different additives had an impact on the morphology of NPs. The hydrocarbon chain influenced the growth process and demonstrated that <10 carbon chain surfactants such as SMS, SOS, did not constitute the CGC and had a minor effect on the mechanism of growth. However, the NPs formation begun at a lower limit indicated as CGC. It was observed only with hydrocarbon chains of > 10 carbon atoms such as AOT, SDS. Fluorescence results confirmed that after laser irradiation, hemi-micelle formation after the development of AgNPs.

Comparison of the Ability of Two Brands of CBCT with That of SEM to Detect the Marginal Leakage of Class V Composite Resin Restorations.

Objectives This study was carried out to compare the ability of two common brands of cone-beam computed tomography (CBCT), including New Tom and Planmeca, to detect the marginal leakage of class V composite resins. The ability of each of the two brands of CBCT to detect the marginal leakage of class V composite resins was also compared with that of scanning electron microscopy (SEM). Methods Class V cavities were prepared on the buccal surface of sixteen extracted caries-free human premolars. Cavities were conditioned and filled with composite resin. The teeth were immersed in 50% weight/weight aqueous silver nitrate solution for 24 hours. They were then taken out and rinsed with distilled water. Next, they were put in a developing solution. They were first viewed with New Tom and Planmeca CBCT units and were then sectioned and evaluated by an SEM. Results The results of the Wilcoxon signed-rank test showed no significant difference between the mean marginal leakage scores of New Tom and Planmeca CBCT images (p value = 0.157) and between those of New Tom CBCT and SEM images (p value = 0.098). However, there was a significant difference between the mean marginal leakage scores of Planmeca CBCT and SEM images (p value = 0.023). Conclusion There were no significant differences between New Tom and Planmeca CBCT units in the detection of marginal leakage of class V composite resins. However, when these CBCT units were compared with the SEM, the New Tom CBCT unit could detect the marginal leakage better than Planmeca.

Deposition of silver on the surface of microparticles of zirconium and molybdenum oxides during their synthesis by laser ablation in liquid

The deposition of silver on the surfaces of micro- and nanoparticles of zirconium, molybdenum, and their oxides, obtained by laser ablation of pure metals in aqueous solutions of silver nitrate, is investigated experimentally. Different irradiation regimes and reagent concentrations are approved. The experimental results indicate a possibility of continuous or partial coating (decorating) with silver the surfaces of nanostructures of oxides of the aforementioned metals; using such systems as substrates, one can increase the Raman scattering amplitude. Silver-coated zirconium and molybdenum oxides can be applied in the diagnostic techniques based on the phenomenon of surface-enhanced Raman scattering.

Green synthesis of silver nanoparticles using Annona squamosa L. seed extract: characterization, photocatalytic and biological activity assay.

The aqueous seed extract of Annona squamosa L. was used as a reducing and stabilizing agent for the synthesis of silver nanoparticles (AgNPs). The formation of AgNPs in aqueous silver nitrate solution after the addition of the extract was indicated by a colour change from pale yellow to dark brown corresponding to a λmax at 430nm. The phytochemicals in the extract, responsible for efficient capping and stabilization of the nanoparticles, were identified by FTIR. Powder XRD pattern demonstrated the polycrystalline nature of the AgNPs. TEM image confirmed that AgNPs were spherical in shape and the average particle size was found to be 22nm. Further, the nanoparticles exhibited good catalytic activity towards the degradation of coomassie brilliant blue dye and demonstrated significant antibacterial activity. Their larvicidal activity against mosquito larvae showed a LC50 value 22.44μg/mL against III instars. In addition, AgNPs positively influenced the germination of chickpea seeds.

Silver Nanoparticles Formation by Nanosecond Pulsed Laser Irradiation in an Aqueous Solution of Silver Nitrate; Effect of Sodium bis (2-ethyl hexyl) Sulfosuccinate

A photochemical reduction of a silver salt precursor using near-ultra-violet (UV) pulsed laser (355 nm) irradiation into aqueous surfactant sodium-bis (2–ethylhexyl) sulfosuccinate (AOT) solution has succeeded in synthesizing homogenous speculative silver nanoparticles (Ag NPs). Without using any additive, the irradiation from ns laser pulses to aqueous silver nitrate solution was observed to create nanocubes (NCs). The photoproduct was transformed into a nanosphere when irradiated with a particular AOT concentration. The photoproduct concentration of NCs to NSs was approximately ten times lower than the critical concentration of micellar (CMC) in AOT, which means that the growth of NSs was aided in a single layer of AOT adsorbed on silver surfaces. A UV / Visible Spectrophotometer and Transmission/Scanning electron microscopy (TEM/SEM) were used to characterize the photochemically synthesized sample thoroughly. The mean size of AgNSs, analyzed by TEM, was 8 nm. These parameters have shown the growth of AgNSs and discussed in the paper. These nanoparticles are potential candidates for catalyst, semiconductor, photovoltaic equipment, medical diagnostics applications than bulk materials.

Growth of porous anodic TiO2 in silver nitrate solution without fluoride: Evidence against the field-assisted dissolution reactions of fluoride ions

Al, Ti, Zr and other metals can be anodized to form metal oxides with a porous structure or a nanotube structure. However, the mechanism of formation of oxide nanopores is still controversial. Anodic TiO2 nanotubes are usually obtained in an electrolyte containing fluoride ions, so a field-assisted dissolution reaction involving fluoride ions is considered to be the main reason for the formation of nanopores. In this paper, titanium was anodized at high current in a silver nitrate solution without fluoride, and a porous titanium oxide structure was obtained, which proves that a field-assisted dissolution reaction involving fluoride ions is not essential for the formation of nanopores. The electronic current leads to oxygen evolution, and the oxygen bubble mold effect is the real reason for the formation of nanopores. These interesting results show that the mechanism of formation of anodized TiO2 nanotubes is the same as that of porous oxide structures. Moreover, the growth rate of nanotubes in an aqueous solution of silver nitrate was much higher than that in a glycol solution of ammonium fluoride at the same voltage.

Effects of Momordica Charantia Silver Nanoparticles on the expressions of Genes Associated With Lipid Metabolism and Nephrotoxicity in Streptozotocin-Induced Rats

Hyperlipidemia and hyperglycemia have been implicated in diabetes mellitus (DM) leading to complications such as nephropathy. Medicinal plants like Mormodica charantia (MC) have been used in the treatment of DM over the years but little is known about their mechanisms of action. This study used biotechnology tools to investigate and compare the effects of M. charantia silver nanoparticles (MCSNPs) with M. charantia extract on expressions of genes linked with nephrotoxicity, lipid and glucose metabolisms using reverse-transcriptase polymerase chain reaction (RT-PCR) in streptozotocin-induced diabetic rats. The genes investigated include kidney injury molecule-1 (KIM-1), 3-hydroxyl, 3-methyl glutaryl_coA reductase (HMG-CoA reductase), peroxisome proliferator-activated receptor alpha and gamma (PPARα and PPARγ). Synthesis of MCSNPs was done using 1 mM concentration of aqueous silver nitrate solution at ratio 1:9 (v/v). Experimental rats were induced intraperitoneally with streptozotocin (65 mg/kg) and divided into six groups viz: diabetic control; normal control; silver nitrate (10 mg/kg); MCSNPs (50 mg/kg); Metformin (100 mg/kg) and M. charantia fraction (100 mg/kg). Sacrifice was done after 12 days of treatment and RT-PCR was then used to investigate gene expressions in liver and kidney tissues of the rats. The expression of HMG-CoA reductase gene was significantly upregulated (p<0.05) upon treatment with 50 mg/kg MCSNPs relative to the diabetic untreated group. M. charantia extracts and MCSNPs significantly upregulate (p<0.05) the expressions of PPAR-α and PPAR-γ compared to the diabetic control. Also, a significant (p<0.05) down-regulation of KIM-1 mRNA expression was observed in MCSNPs- treated group, relative to the diabetes untreated group. M. charantia silver nanoparticles could be a potent antidiabetic agent due to its potential to modulate genes associated with lipid metabolism and nephrotoxicity.
 Keywords: Medicinal plant; Diabetes Mellitus; Silver Nanoparticles; nephrotoxicity; gene expression

Selective electrooxidation of acetaldehyde in aqueous ethanol alkaline solutions on silver-containing electrodes

Selective electrochemical oxidation of acetaldehyde in aqueous alkaline ethanol solutions on the silver-containing electrodes has been presented. Several types of silver-containing electrode were used: the smooth silver disk electrode, rough silver electrode and the glassy carbon electrode modified with the silver-polypyrrole composite ink. Silver-polypyrrole (Ag-PPy) composite was synthesized via single-step chemical oxidation with the use of diluted aqueous solutions of silver nitrate and pyrrole in molar ratio 1:50. It has been shown that acetaldehyde is oxidized selectively on silver in the presence of ethanol in alkaline solutions. The influence of various factors (pH, the presence of molecular oxygen, ethanol and acetate anions, surface area extension and polypyrrole presence) on the aldehyde electrooxidation process has been studied. It was shown that for the silver-polypyrrole composites the aldehyde electrooxidation peak potential is shifted to negative direction in comparison with the silver electrode. However, the sensitivity of the silver-polypyrrole composite modified electrode is lower than for smooth or rough silver electrode. The addition of nitrate anions in alkaline solution permits to increase the peak currents for Ag-PPy modified electrode.

Brief descriptions of the principles of prominent methods used to study the penetration of materials into human hair and a review of examples of their use.

Consumers are attracted to the latest fashion trends and different looks. This drives the search for novel hair treatments. Some chemicals present in hair treatment products can penetrate the hair shaft. These materials can either nourish or injure the hair cortex. Different techniques have been used to investigate the mechanism of molecule penetration and the conditions under which penetration occurs. This article reviews the techniques applied for this purpose. Various microscopy techniques are used to capture clear and colourful images to determine the diffusion pathways and the exact location of the molecules under study. However, the laborious sample preparation often leads to sample destruction since cross-sectioning is often required. While various other techniques have been successfully used for investigating the penetration methods, most of these require different amounts of work to be put in for sample preparation and instrumentation. Several spectroscopic techniques have been used to study the penetration of the molecules because of the high levels of accuracy and the quick response time of these techniques. Moreover, the samples are not damaged during the investigation.

Biosynthesis and Characterization of Silver Nanoparticles Using Wheatgrass Extract and Assessment of Their Anticandidal Activity

Wheatgrass extract was used for eco-friendly extracellular synthesis of silver nanoparticles. Stable silver nanoparticles were formed by treating the wheatgrass extract with aqueous silver nitratesolution as reducing agents. Ultraviolet–visible spectroscopy was used to confirm the presence of silver nanoparticles. The X-ray diffraction analysis exhibited characteristic peaks signifying the crystalline nature of nanoparticels. The particle size and the involvement of various biomolecules in the synthesis of silver nanoparticles were determined using Atomic force microscopy and Fourier transform infrared spectroscopy, respectively. The particle structure was studied by scanning electron microscopy and the elemental silver present in reaction mixture was confirmed by Energy dispersive x-ray. The nanoparticles were tested for their anticandidal activity against Candida albicans MTCC3017, Candida albicans MTCC1637, Candida albicans MTCC183, Candida tropicalis MTCC230 and Candida glabrata MTCC3814 and determined their minimum inhibitory concentration.