Aqueous Soluble Partitionates Research Articles

Studies of Biological Activities of the Roots of Bombax ceiba L.

Bioactivities of the methanolic crude extract of root of Bombax ceiba L. and its organic and aqueous soluble partitionates were studied to investigate its medicinal importance. In Free radical scavenging assay by DPPH method, the aqueous soluble partitionate (AQSF) demonstrated the highest free radical scavenging activity with IC50 value of 3.33 ± 0.25 μg/ml. The brine shrimp lethality bioassay of different partitionates of the root demonstrated the significant lethality by the hexane soluble partitionate (HSF) having LC50 value of 1.19 ± 0.10 µg/ml. Among different fractionates of the root, the highest percentage (44.55 ± 0.12%) of clot lysis was exhibited by AQSF. The crude extract revealed promising antidiarrheal, hypoglycemic, central and peripheral analgesic activities at both doses of 200- and 400- mg/kg body weight in Swiss-Albino rat model. Bangladesh Pharmaceutical Journal 22(2): 219-223, 2019

In vitro Study of Different Partitionates of Alstonia scholaris (L.) R.Br. Leaf for Thrombolytic and Membrane Stabilizing Activities

Alstonia scholaris (L.) R.Br. is a medicinal herb belonging to the family Apocyanaceae. Locally it is known as Chatim and abundant with ethno-medicinal properties. The crude ethanolic extract of A. scholaris leaves along with its n-hexane, ethyl acetate, dichloromethane and aqueous soluble partitionates were assayed for their probable thrombolytic and membrane stabilizing activities. The activities of the leaf extract were compared to standard drugs, streptokinase for thrombolytic and acetyl salicylic acid for membrane stabilizing activities. In this study, among all extractives the highest thrombolytic activity was exhibited by the crude ethanolic extract (55.33±0.08%) (p < 0.001) as compared to Streptokinase (62.07±0.33%) (p < 0.001). Alternatively, ethanol soluble materials also significantly inhibited the haemolysis of human erythrocyte membrane both in induced by hypotonic solution (83.47±0.15%) (p < 0.001) and by heat (79.82±0.71%) (p < 0.001), respectively as compared to standard acetyl salicylic acid (85.09±0.8%) (p < 0.001) and (80.98±1.34%) (p < 0.001).Bangladesh Pharmaceutical Journal 21(2): 145-149, 2018

English

The crude methanol extract of leaves of Jasminum matthewii as well as its hexane, carbon tetrachloride, chloroform and aqueous soluble partitionates were subjected to screening for antioxidant, cytotoxic, thrombolytic, membrane stabilizing, antimicrobial, analgesic, anti-diarrheal and central nervous system depressant activity assays. The antioxidant potential was evaluated in terms of total phenolic content and free radical scavenging activity using butylated hydroxytolune (BHT) and ascorbic acid as standards. Among the test samples of J. matthewii, the highest free radical scavenging activity was demonstrated by the aqueous soluble fraction (IC50= 41.55±0.51 µg/ml), whereas in case of brine shrimp lethality bioassay, the hexane soluble fraction revealed the highest cytotoxic activity with LC50 value 0.19±0.32 μg/ml. In thrombolytic activity assay, the aqueous soluble fraction of J. matthewii extractives showed 62.29±0.29% of clot lysis, whereas standard streptokinase demonstrated 66.77% clot lysis. Among the test samples, the crude methanol extract inhibited 70.15±0.39% haemolysis of red blood cells induced by hypotonic solution. In case of heat-induced condition, the aqueous soluble fraction demonstrated 25.25±0.31% inhibition of haemolysis of red blood cells. None of the test samples revealed any zone of inhibition in disc diffusion assay. In peripheral analgesic activity assay, the crude methanol extract of J. matthewii demonstrated 51.02% inhibition of writhing at a dose of 400 mg/kg body weight dose compared to 74.49% inhibition by standard diclofenac sodium. In anti-diarrheal activity assay, the methanolic crude extract reduced diarrheal feces by 89.00±0.15% at 400 mg/kg dose. J. matthewii extractives potentiated phenobarbitone sodium-induced sleeping time in a dose dependent manner. Key words: Jasminum matthewii, free radical scavenging activity, brine shrimp lethality, thrombolysis, membrane stabilization, hypotonic solution, zone of inhibition, writhing.

Exploration of In-vitro antimicrobial and cytotoxic potentials of crude methanolic extracts of Quisqualis indica (stem bark): An ethno medicinal plant

Quisqualis indica plant (Stem bark) was analyzed in-vitro to evaluate biological activities including anti-microbial properties and cytotoxic potentials. The crude n-hexane, carbon tetrachloride, chloroform and aqueous soluble partitionates of the methanolic extract of the plant were subjected to the microbiological investigation against a wide range of Gram positive, Gram negative bacteria and fungi. Brine shrimp lethality bioassay was used for evaluation of cytotoxic activity of Quisqualis indica. The zones of inhibition produced by the hexane, carbon tetrachloride, chloroform and aqueous soluble partitionates of the methanolic extract of Quisqualis indica were ranged from 7-15 mm, 8-18 mm, 12-20 mm and 10-16 mm respectively at a concentration of 400μg/disc. The obtained results were compared against standard Kanamycin (30µg/disc). In case of brine shrimp lethality bioassay, the n-hexane, carbon tetrachloride, chloroform and aqueous soluble partitionates were found to show LC50 of 1.254, 0.826, 3.866 and 5.366 µg/ml respectively as compared with vincristine sulfate (with LC50 value of 0.3229 µg/ml) as a standard positive control. The obtained results confirmed the presence of anti-microbial properties and cytotoxic potentials of Quisqualis indica which supports the traditional use of this plant in various diseases caused by pathogenic microorganisms and can be a good candidate for a further anti-tumor drug study.

Wild Cinchona (Neolamarckia cadamba) – Bioactivities of a Medicinal Plant of Bangladesh

The methanol extract of bark of Neolamarckia cadamba (Roxb.) and its organic and aqueous soluble partitionates were subjected to assays for antioxidant, cytotoxic, thrombolytic, membrane stabilizing, antimicrobial and analgesic activities. In the DPPH free radical scavenging assay, the pet ether and carbon tetra chloride soluble partitionate of the methanolic extract demonstrated the highest free radical scavenging activity with IC50 values of 60.46 ?g/ml and 78.58 ?g/ml, respectively. On the other hand, the carbon tetrachloride and pet ether soluble fractions displayed the potential lethality to brine shrimps, having LC50 of 7.24 and 7.89 ?g/ml, as compared to standard vincristine sulphate (LC50 value of 0.45 ?g/ml). During assay for thrombolytic property, the carbon tetrachloride and aqueous soluble materials revealed 66.36 % and 64.25 % clot lysis of human blood, respectively. In the membrane stabilizing assay, the carbon tetrachloride soluble fraction inhibited 86.79 % haemolysis of human RBCs in hypotonic solution-induced condition, while the aqueous soluble partitionate inhibited 92.39 % haemolysis of RBCs in the heat-induced condition. The crude methanolic extract of bark of N. cadamba showed significant central and peripheral analgesic activity at both 200 and 400 mg/kg body weight, whereas its chloroform soluble fraction mildly inhibited the growth of test microorganisms. Therefore, our studies suggest that wild cinchona should be subjected to extensive phytopharmacological investigation.Bangladesh Pharmaceutical Journal 19(1): 32-36, 2016

Cytotoxic, thrombolytic, membrane stabilizing and anti-oxidant activities of <i>Hygrophila schulli</i>

<p class="Abstract">The crude methanol extract of Hygrophila schulli and its petroleum ether, chloroform, ethyl acetate and aqueous soluble Kupchan partitionates were investigated for in vitro cytotoxic, thrombolytic, membrane stabilizing and anti-oxidant activities. In the brine shrimp lethality bioassay, the petroleum ether soluble extract of H. schulli showed significant cytotoxicity (LC<sub>50</sub> = 0.1 µg/mL and LC<sub>90</sub> = 15 µg/mL) as compared to vincristine sulfate (LC<sub>50</sub> = 0.4 µg/mL and LC<sub>90</sub> = 9 µg/mL). Among all the partitionates, chloroform soluble fraction demonstrated the highest thrombolytic activity with 10.5% clot lyses. Moreover, in hypotonic- and heat-induced conditions, the chloroform soluble extractive inhibited hemolysis of human erythrocyte by 113.7% and 14.3%, respectively as compared to 71.9% and 42.2% demonstrated by standard acetylsalicylic acid. On the other hand, in anti-oxidant activity test, chloroform soluble fraction revealed mild antioxidant activity (IC<sub>50</sub> = 195.1 µg/mL) as compared to standard tert-butyl-1-hydroxytoluene (IC<sub>50</sub> = 27.5 µg/mL).</p><p> </p>

Pharmacological Activities of Grevillea robusta, a Medicinal Plant of Bangladesh

The objective of this study was to evaluate the crude methanol extract of leaf of Grevillea robusta as well as its hexane, carbon tetrachloride, chloroform and aqueous soluble partitionates for cytotoxic, thrombolytic, membrane stabilizing and antimicrobial activities. In the brine shrimp lethality bioassay, the crude methanolic extract of G. robusta leaf revealed the highest cytotoxic activity with LC50 values as 1.50 ± 0.45 ?g/ml as compared to 0.45 ?g/ml for vincristine sulphate. Among the extractives of G. robusta, the carbon tetrachloride soluble fraction showed 69.95±0.11% clot lysis as compared to 70.77% clot lysis by standard streptokinase. At concentration of 1.0 mg/ml, the chloroform soluble fraction inhibited 40.31 ± 0.59% and 62.93 ± 0.73% of haemolysis of RBC induced by heat and hypotonic solution as compared to 42.12% and 71.90% by acetyl salicylic acid, respectively. The test samples also showed antimicrobial activity with zone of inhibition ranging from 7.0 to 17.0 mm in diameter. The chloroform soluble partitionate demonstrated the highest zone of inhibition (17.0 mm) against Salmonella Typhi. DOI: http://dx.doi.org/10.3329/bpj.v17i2.22329 Bangladesh Pharmaceutical Journal 17(2): 135-137, 2014

Bioactivities of Malvaviscus arboreus var. drummondii and Phyllanthus reticulatus Poir.

The study was designed to evaluate the bioactivities of crude methanol extracts of leaves of Malvaviscus arboreus and Phyllanthus reticulatus along with their pet-ether, carbon tetrachloride, chloroform and aqueous soluble partitionates. The antioxidant potential was evaluated by DPPH and Folin-Ciocalteau reagents using butylated hydroxytolune (BHT) and ascorbic acid as standards. In the DPPH free radical scavenging assay, the crude methanol extract of M. arboreus revealed the highest free radical scavenging activity with IC50 values 6.47 ± 0.78 ?g/ml. In brine shrimp lethality bioassay, the chloroform soluble materials of M. arboreus displayed the highest cytotoxic potential having LC50 values 2.73 ± 0.36 ?g/ml, whereas the standard vincristine sulphate exhibited an LC50 value of 0.45 ?g/ml. In the thrombolytic activity assay, the pet-ether soluble fractions of M. arboreus and P. reticulatus revealed 55.60 ± 0.48 % and 18.99 ± 0.84% clot lysis, respectively. In the membrane stabilizing activity assay, the chloroform soluble fraction of M. arboreus inhibited heat- and hypotonic solution- induced haemolysis of RBCs by 54.60 ± 0.81 % and 66.96 ± 0.84 % as compared to 57.06 % and 72.79 % inhibition by acetyl salicylic acid (0.10 mg/ml), respectively. On the other hand, the pet-ether soluble materials of crude methanol extract of P. reticulatus demonstrated 57.11 ± 0.57 % and 70.62 ± 0.22 % inhibition of heat- and hypotonic solution- induced hemolysis, respectively. DOI: http://dx.doi.org/10.3329/dujps.v13i2.21892 Dhaka Univ. J. Pharm. Sci. 13(2): 143-147, 2014 (December)

English

The methanol extract of the leaf of Allamanda cathartica L. as well as its hexane, carbon tetrachloride, chloroform and aqueous soluble partitionates were subjected to screening for antioxidant, membrane stabilizing and antimicrobial activities. The antioxidant potential was evaluated by 1,1-diphenyl-2-picrylhydrazyl (DPPH) and Folin-Ciocalteau reagents using butylated hydroxytolune (BHT) and ascorbic acid as standards. The carbon tetrachloride soluble fraction revealed the highest free radical scavenging activity (IC50 = 47.5±0.11 μg/ml) which could be correlated to its total phenolic content of 59.31±0.47 mg of gallic acid equivalent (GAE)/g of extractives. In hypotonic solution and heat induced conditions, the aqueous soluble fraction inhibited haemolysis of human erythrocyte by 69.49±0.49 and 40.0±0.75%, respectively. Here, acetyl salicylic acid (0.1 mg/ml) was used as reference showing 72.79 and 42.12% of haemolysis of red blood cells (RBCs) in hypotonic solution and heat induced conditions, respectively. The carbon tetrachloride soluble fraction of A. cathartica demonstrated activity against microbial growth with zone of inhibition ranging from 5.0 to 8.5 mm. This fraction demonstrated 8.5 mm zone of inhibition against Bacillus megaterium. Key words: Allamanda cathartica, total phenolic content, 1,1-diphenyl-2-picrylhydrazyl (DPPH), free radical scavenging activity, membrane stabilizing activity, zone of inhibition.

Membrane stabilization – A possible mechanism of action for the anti-inflammatory activity of a Bangladeshi medicinal plant: Erioglossum rubiginosum (Bara Harina)

AimsThe present study aimed at assessing the effect of methanol extract of Erioglossum rubiginosum, a widely used shrub like plant in folkloric medicine, in experimentally induced inflammation, using human red blood cell (HRBC) membrane stabilization as study method. MethodsThe methanol extract of leaves of E. rubiginosum and its pet-ether, carbon tetrachloride, chloroform and aqueous soluble partitionates were subjected to assays for lysis of erythrocytes and % of inhibition in hypotonic solution and heat induced lysis, using acetyl salicylic acid as standard drug, in an in vitro model. ResultsAt the concentration of 1 mg/ml, methanol extract and its pet-ether, carbon tetrachloride, chloroform and aqueous soluble partitionates significantly inhibited hypotonic solution induced lysis of the human red blood cell membrane with values of 0.428 ± 0.005, 0.204 ± 0.003, 0.233 ± 0.002, 0.411 ± 0.003, 0.439 ± 0.003% respectively; which were comparable to the standard drug acetyl salicylic acid (0.1 mg/ml), 0.166 ± 0.003. In case of heat induced HRBC heamolysis, the plant extracts also showed significant activity where the values of inhibitory actions were 14.46 ± 0.344, 28.23 ± 0.315, 25.18 ± 0.303, 17.09 ± 0.365, 13.44 ± 0.470% for methanol extract, pet-ether, carbon tetrachloride, chloroform and aqueous soluble partitionates respectively. ConclusionIt can be postulated from the observed results that the membrane stabilizing action and inhibition of erythrocyte lysis property of E. rubiginosum may be the possible mechanism of action of its anti-inflammatory activity. So, further studies are suggested to evaluate the anti-inflammatory and analgesic activities of the plant.

Bioactivities of Artocarpus chaplasha Roxb. and Bougainvillea spectabillis Willd.

The methanol extracts of leaf of Artocarpus chaplasha Roxb. and Bougainvillea spectabillis Willd. as well as their petroleum ether, carbon tetrachloride, chloroform and aqueous soluble partitionates were subjected to screenings for antioxidant, cytotoxic, thrombolytic, membrane stabilizing and antimicrobial activities. The antioxidant potential was evaluated by 1,1-diphenyl-2-picrylhydrazyl (DPPH) and Folin- Ciocalteau reagent using butylated hydroxytolune (BHT) and ascorbic acid as standards. The aqueous soluble fraction of the crude methanol extract of A. chaplasha and the methanolic crude extract of B. spectabillis demonstrated the highest free radical scavenging activity with the IC50 of 15.60±0.27 ?g/ml and 18.33±0.17 ?g/ml, which could be correlated to their total phenolic content of 61.26±0.23 and 50.00±0.19 mg of GAE/gm of extractive, respectively. Different extractives of A. chaplasha and B. spectabillis showed clot lysis activity ranging from 2.56±0.68% to 17.07±0.23% and 2.24±0.71% to 17.59±0.74% as compared to 66.77% and 3.791 % produced by the standard streptokinase and water, respectively. In hypotonic solution and heat induced conditions, the crude extract of A. chaplasha inhibited the haemolysis of human erythrocyte by 41.96±0.87% and 40.00±0.78%, while the carbon tetrachloride soluble fraction of methanol extract of B. spectabillis inhibited haemolysis by 65.68±0.36% and 35.62±0.82%, respectively. Here, acetyl salicylic acid (0.1 mg/ml) was used as reference showing 72.79% and 42.12% of haemolysis of RBCs in hypotonic and heat induced conditions, respectively. Among the extractives of both plants, the crude methanol extract of A. chaplasha and the chloroform soluble fraction of B. spectabillis extract revealed mild to moderate antimicrobial activity with zone of inhibition ranging from with 7.0 to 13.0 mm. The general toxicity was determined by brine shrimp lethality bioassay where the pet-ether soluble fraction of A. chaplasha (LC50 0.781±0.36 ?g/ml) and the aqueous soluble partitionate of B. spectabillis (LC50 1.28±0.57 ?g/ml) suggested the presence of considerable bioactive principles. DOI: http://dx.doi.org/10.3329/bpj.v16i1.14493 Bangladesh Pharmaceutical Journal 16(1): 63-68, 2013

Membrane Stabilizing and Preliminary Hypoglycemic Activities of Picrasma javanica

The methanol extract of leaves of Picrasma javanica Blume and its n-hexane, carbon tetrachloride, chloroform and aqueous soluble partitionates were evaluated for membrane stabilizing and preliminary hypoglycemic activities. In hypotonic solution and heat-induced conditions, the crude methanol extract inhibited 65.30±0.52% and 36.79±0.38% haemolysis of RBC as compared to 72.79% and 42.12% produced by standard drug, acetyl salicylic acid, respectively (0.10 mg/ml). The crude extract also showed blood sugar lowering efficacy at 200 and 400 mg/ kg body weight, with 48.63% reduction of blood sugar level at 400 mg/kg body weight while the standard drug, Glibenclamide (10 mg/kg body weight) demonstrated 53.7% reduction of blood sugar level at 3rd hour of administration. DOI: http://dx.doi.org/10.3329/bpj.v16i1.14500 Bangladesh Pharmaceutical Journal 16(1): 89-92, 2013

Antioxidant and cytotoxic activities of Parabaena sagitatta Miers

A methanolextract of the leaves of Parabaena sagitatta Miers and its petroleum ether, carbon tetrachloride, dichloromethane, ethylacetate and aqueous soluble partitionates were evaluated for antioxidant activity by 1,1-diphenyl-2-picrylhydrazyl (DPPH), Folin-Ciocalteau reagent and phosphomolybdenum total antioxidant assays by using butylated hydroxytolune (BHT) and ascorbic acid as standards. The dichloromethane soluble fraction demonstrated the presence of significant amount of phenolic compounds 61.06 ± 0.54 mg GAE/g of extract and also has moderate antioxidant activity IC50 50.62 ± 0.25 ?g/ml. A positive correlation (R2), 0.969 was observed between total phenolic content and total antioxidant activity of P. sagitatta. The general toxicity was determined by brine shrimp lethality bioassay where the dichloromethane LC50DOI: http://dx.doi.org/10.3329/bjb.v41i2.13441 Bangladesh J. Bot. 41(2): 155-158, 2012 (December)

In Vitro Membrane Stabilizing and Thrombolytic Activities of Ophirrhiza mungos, Mussaenda macrophylla, Gmelina philippensis and Synedrella nodiflora Growing in Bangladesh

The methanol extracts and their pet-ether, carbon tetrachloride, chloroform and aqueous soluble partitionates of Ophirrhiza mungos, Mussaenda macrophylla, Gmelina philippensis and Synedrella nodiflorawere subjected to assays for membrane stabilizing and thrombolytic activities. The extractives inhibited heat- as well as hypotonic solution-induced haemolysis of human erythrocytes in vitro. The pet-ether soluble fraction of O. mungos, M. macrophylla and S. nodiflora demonstrated 61.16 % & 24.75%, 52.55% & 23.35% and 60.24% & 22.85% inhibition of hemolysis of RBC caused by hypotonic solution and heat, whereas the carbon tetrachloride soluble fraction of G. philippensis showed 49.05% and 21.25% inhibition of hypotonic and heat induced hemolysis of RBC, respectively. Here, acetyl salicylic acid was used as reference standard at 0.10 mg/mL. Among the four plants, the carbon tetrachloride soluble fraction of O. mungos, methanol extract of M. macrophylla, carbon tetrachloride soluble fraction of G. philippensis and chloroform soluble fraction of S. nodiflora revealed highest thrombolytic activity with clot lysis value of 50.09%, 49.50%, 47.14% and 46.37%, respectively. Standard streptokinase and water were used as positive and negative controls which showed 65.00% and 3.84% lysis of clot, respectively.

In vitro Antioxidant, Membrane Stabilizing and Thrombolytic Activities of Glycosmis arborea

The methanol extract of leaves and stems of Glycosmis arborea and their pet-ether, carbon tetrachloride, chloroform and aqueous soluble partitionates were subjected to assays for antioxidant activity by Folin-Ciocalteau reagent, membrane stabilizing and thrombolytic activities. The carbon tetrachloride soluble fraction of stems of G. arborea demonstrated the presence of significant amount of phenolic compounds (36.95 ± 0.54 mg of GAE/g of extract). The extractives inhibited heat as well as hypotonic solution-induced haemolysis of rat erythrocytes in vitro. The pet-ether soluble fraction of leaves and stems of G. arborea showed 20.46 and 38.24% and 22.50 and 48.25%. inhibition of hemolysis of RBC caused by heat and hypotonic solution, as compared to 30.55 and 72.91% inhibition of hemolysis of RBC caused by the standard Acetyl salicylic acid at 0.01 mg/ml concentration, respectively. Among the four fractions, the chloroform soluble materials of the stems of G. arborea revealed highest thrombolytic activity with clot lysis value of 36.50% while standard streptokinase and water used as positive and negative controls, showed 64.25±0.26 and 2.35±0.35 % lysis of clot respectively DOI: http://dx.doi.org/10.3329/bpj.v15i2.12579 Bangladesh Pharmaceutical Journal 15(2): 141-143, 2012

Antioxidant, Thrombolytic and Cytotoxic Activities of Picrasma javanica

The methanol extract of leaf of Picrasma javanica as well as its n-hexane, carbon tetrachloride,chloroform and aqueous soluble partitionates were subjected to screening for antioxidant and thrombolytic activities and brine shrimp lethality. The antioxidant potential was evaluated by DPPH, Folin-Ciocalteau reagent and phosphomolybdenum total antioxidant assays using butylatedhydroxytolune (BHT) and ascorbic acid as standards. All fractions showed moderate to strong antioxidant activity, of which the chloroform and aqueous soluble fractions demonstrated the highest activity with the IC50 value of 14.59 ± 0.73 ?g/ml and 18.6 ± 0.15 ?g/ml, respectively. The total phenolic content of the chloroform and aqueous soluble fractions was 10.4 ± 0.64 and 5.15 ± 0.22 mg of GAE/g of extractive, respectively. Thus, a positive correlation was found between the total phenolic content and total antioxidant activity of P. javanica. The general toxicity was determined by brine shrimp lethality bioassay where the crude extract (LC50 1.04 ± 0.31 ?g/ml) and its n-hexane (LC50 1.28 ± 0.45?g/ml) soluble partitionate demonstrated the presence of considerable bioactive principles. Mild to moderate thrombolytic activity was discerned by the methanol extract of leaf of P. javanica and its different fractions. During assay for thrombolytic activity, the carbon tetrachloride soluble materials revealed 34.165±1.57 % of clot lysis while standard streptokinase and water, used as positive and negative controls, demonstrated 66.77% and 3.791% lysis of clot, respectively. DOI: http://dx.doi.org/10.3329/dujps.v11i1.12491 Dhaka Univ. J. Pharm. Sci. 11(1): 71-74, 2012 (June)

Antimicrobial activity and toxicity of Quisqualis indica

The plant Quisqualis indica (Compositae) has ehnopharmcological reputation of being used as a healing agent in Bangladesh. In this study, preliminary screenings were conducted to look at the antimicrobial susceptibility and cytotoxicity of the plant extract. The extractives of the plant were subjected to screening for inhibition of microbial growth by the disc diffusion method. The zones of inhibition demonstrated by the n-hexane, carbon tetrachloride, chloroform and aqueous soluble partitionates of the methanolic extract ranged from 8 - 15 mm, 8 - 18 mm, 12 - 20 mm and 10 - 16 mm, respectively at a concentration of 400 <TEX>${\mu}g$</TEX>/disc. All the extractives were also subjected to brine shrimp lethality bioassay for primary cytotoxicity evaluation. Here, the carbon tetrachloride soluble materials demonstrated the highest cytotoxicity with <TEX>$LC_{50}$</TEX> of 0.826 <TEX>${\mu}g$</TEX>/ml, while n-hexane, chloroform and aqueous soluble partitionates of the methanolic extract revealed the <TEX>$LC_{50}$</TEX> of 1.254, 3.866 and 5.366 <TEX>${\mu}g$</TEX>/ml, respectively. This is the first report of the antimicrobial activity and cytotoxicity Q. indica.