Abstract
The methanol extract of the leaf of Allamanda cathartica L. as well as its hexane, carbon tetrachloride, chloroform and aqueous soluble partitionates were subjected to screening for antioxidant, membrane stabilizing and antimicrobial activities. The antioxidant potential was evaluated by 1,1-diphenyl-2-picrylhydrazyl (DPPH) and Folin-Ciocalteau reagents using butylated hydroxytolune (BHT) and ascorbic acid as standards. The carbon tetrachloride soluble fraction revealed the highest free radical scavenging activity (IC50 = 47.5±0.11 μg/ml) which could be correlated to its total phenolic content of 59.31±0.47 mg of gallic acid equivalent (GAE)/g of extractives. In hypotonic solution and heat induced conditions, the aqueous soluble fraction inhibited haemolysis of human erythrocyte by 69.49±0.49 and 40.0±0.75%, respectively. Here, acetyl salicylic acid (0.1 mg/ml) was used as reference showing 72.79 and 42.12% of haemolysis of red blood cells (RBCs) in hypotonic solution and heat induced conditions, respectively. The carbon tetrachloride soluble fraction of A. cathartica demonstrated activity against microbial growth with zone of inhibition ranging from 5.0 to 8.5 mm. This fraction demonstrated 8.5 mm zone of inhibition against Bacillus megaterium. Key words: Allamanda cathartica, total phenolic content, 1,1-diphenyl-2-picrylhydrazyl (DPPH), free radical scavenging activity, membrane stabilizing activity, zone of inhibition.
Highlights
Allamanda cathartica L. (Synonyms: Echites verticillata Sessé & Moç, Orelia grandiflora Aublet, Allamanda grandiflora (Aublet) Poiret in Lam, Allamanda hendersonii W
An aliquot (5 g) of the concentrated methanol extract was fractionated by modified Kupchan partition protocol and the resultant partitionates were evaporated to dryness with rotary evaporator to yield hexane (HXSF, 1.0 g), carbon tetrachloride (CTCSF, 1.2 g), chloroform (CSF, 1.5 g) and aqueous (AQSF, 1.0 g) soluble materials
Following the method developed by Brand-Williams et al (1995), the antioxidant activity of the test samples was assessed by evaluating the scavenging activities of the stable 1,1-diphenyl-2picrylhydrazyl (DPPH) free radical by using synthetic antioxidants, butylated hydroxytoluene (BHT) and ascorbic acid as positive controls
Summary
Allamanda cathartica L. (Synonyms: Echites verticillata Sessé & Moç, Orelia grandiflora Aublet, Allamanda grandiflora (Aublet) Poiret in Lam, Allamanda hendersonii W. As part of our ongoing investigations on medicinal plants of Bangladesh (Kaisar et al, 2011; Sharmin et al, 2012), the methanol extract of leaves of A. cathartica as well as its organic and aqueous soluble fractions were studied for the antioxidant potential in terms of total BHT: Butylated hydroxytolune; ME: methanolic crude extract; HXSF: hexane soluble fraction; CTCSF: carbon tetrachloride soluble fraction; CSF: chloroform soluble fraction; AQSF: aqueous soluble fraction.
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