Aqueous Extract Of Pomegranate Peel Research Articles

Evaluating the Anti-Cancer Effect of Pomegranate Aqueous Extract of Gold Nanoparticles by Using Photodynamic Laser on Laboratory Mice.

Background: This study evaluated the anticancer effects of gold nanoparticles (Au- NPs) generated by reducing hydrated gold metal ions in the presence of aqueous pomegranate peel extract. Aim of study: To test the possibility that Au-NPs generated from the pomegranate aqueous extract could be employed as an anti-cancer therapy. Materials and methods: The study aimed to evaluate Photodynamic therapy (PDT) using a laser at a wavelength of 532 nm with green gold particles as a photosensitizer in in-vivo experiment. Gold nanoparticles (Au-NPs) were produced by reducing hydrated gold metal ions in the presence of aqueous pomegranate peel extract. Using UV-visible spectroscopy, the absorption peak of the gold particles was found near 550- 592 nm. All diffraction peaks for 2θ at = 37.8227º at 43.7014º, 63.8103º and 77.1540º correspond to the (111), (200), (220), and (311) levels, respectively by X-ray diffraction(XRD) analysis, imaging confirmed Au-NP by Transmission electron microscopy (TEM), the nanoparticle size ranged between 19.96 ± 0.784 nm. Forty Mature Swiss females weighing 20–25 g and aged 6–8 weeks were used, the animals were placed in well-ventilated rooms. Results: The best exposure time to eradicate the tumor is with a duration of exposure of 30 minutes using the diode laser, as histological investigations indicated the development of anti-immunity with the same exposure period.

Antibacterial Potential of Pomegranate Peel Extracts on Escherichia coli Isolated From Benha Hospital in Egypt

Introduction. Pomegranate is an old fruit which has many antimicrobial compounds. Impact of pomegranate peel extracts on Escherichia coli clinical isolate from Benha general hospital was investigated in this study. Material and Methods: pomegranate peel extraction was primed and GC/MS chromatogram analysis was carried out using the GC Agilent Technologies gas chromatography fitted with HP-5 fused silica column (25mm × 0.25mm, film thickness of 0.25 µm) and interfaced to the flame-ionization detector (FID). The antibacterial potential of Pomegranate peel extractions against E.coli sample was done using well diffusion method. Results: According to (GC/MS) technique, methanol and aqueous extracts of pomegranate peel have inhibitory effects on E. coli isolate. The minimum inhibition concentrations (MICs) of methanolic and aqueous extracts were 100 and 60 mg/L, respectively. In this study , the MICs values of Aqueous extract of pomegranate peel were higher than of methanolic extract of pomegranate peel (P<0.05). Zones of inhibition and values of MICs indicated that the aqueous extract of pomegranate peel had more effect on E. coli isolate than methanolic extract of pomegranate peel. Conclusion: In this study, the extraction of pomegranate peel has antibacterial effect on E.coli isolate. However, the aqueous extract had a higher effect on E.coli than the methanolic extract.

An aqueous pomegranate peel extract (Punica granatum) protect against Elastase-induced pulmonary emphysema in Sprague Dawley rats model

We investigated the effect of Punica granatum peel aqueous extract (PGE), on pulmonary inflammation and alveolar degradation induced by intratracheal administration of Elastase in Sprague Dawley rats. Lung inflammation was induced in rats by intratracheal instillation of Elastase. On day 1 and 2, animals received an intraperitoneal injection of PGE (200 mg/mL), three hours later, they were intratracheally instilled with 25U/kg pancreatic porcine Elastase. Animals were sacrificed 7 days later. Bronchoalveolar lavage (BAL) were collected and cellularity, histology and mRNA expression of Monocyte chemotactic protein 1(MCP-1), Tumor Necrosis Factor-Alpha (TNF-α), Interleukin 6 (IL-6), and Matrix Metalloproteinase-2 (MMP-2) were studied. In addition, activity of TNF- α, IL-6 and MCP-1 on BAL were also analyzed by ELISA Kit. Elastase administration increased: BAL cellularity, neutrophils recruitment and BAL MCP1, IL-6 expressions. It also increased lung TNF-α, MCP-1, MMP-2 expressions, platelets recruitment, histological parameters at 7th day of elastase treatment. Intraperitoneal injection of 200 mg/kg of PGE reduced, significantly, BAL cellularity, and neutrophils recruitment. However, in animal treated with PGE, MCP-1, MMP-2 and IL-6 on day 7, were similar to the Sham group. Treatment with PGE (200 mg/ kg) also significantly reduced lung TNF-α, and MCP-1 expression. This study reveals that PGE Punica granatum protects against elastase lung inflammation and alveolar degradation induced in rats.

Pomegranate peel extract protects against carbon tetrachloride-induced nephrotoxicity in mice through increasing antioxidants status.

Carbon tetrachloride (CCl4) is a notorious environmental pollutant known for its toxicity. The aim of the present study was to evaluate the possible protective effects of aqueous pomegranate peel extract (PPE) against CCl4 induced nephrotoxicity in mice. Adult male mice were divided into four groups: Group one was used as the control; Group two was treated with a daily oral dose of PPE (400 mg/kg) for 15 days; the third group was intraperitoneally injected with a dose (1 ml/kg) of CCl4 twice a week for two weeks; and the final group was injected with the same dose of CCl4 twice a week concomitantly with a daily oral dose of PPE (400 mg/kg). Biochemical and histopathological data were analyzed along with the gene expression levels of the antioxidant enzymes and immunohistochemistry of the kidney tissue. CCl4 resulted in a significant increase in the serum urea and creatinine levels with detectable degenerative changes in the Bowman's capsule and glomerulus, with cells exhibiting vacuolization and evidence of necrosis. Co-administration of animals with CCl4 and PPE resulted in improved biochemical and histopathological conditions. Similarly, increased production of the Caspase-3 and collagen fibers were reduced in mice treated with PPE. Quantitative analysis of superoxide dismutase, catalase and glutathione peroxidase further accentuated the effects of PPE treatment significantly improving the conditions of the CCl4-administered group. The results of the present study demonstrate that the phenolic derivative rich PPE is a potent nephroprotective agent and suppresses CCl4-induced nephrotoxicity in mice.

Antimicrobial activity of the aqueous extract of pomegranate peel and Lantana cammara leaves extracts against Proteus mirabilis

Antimicrobial activity of the aqueous extract of pomegranate peel and Lantana cammara leaves extracts against Proteus mirabilis

Green Synthesis of Zinc Oxide Nanoparticles from (Punica granatum L) Pomegranate Aqueous Peel Extract

The biosynthesis of Zinc oxide nanoparticles (ZnONPs) was done under alkaline conditions (pH 12) and temperature ≥ 80 ◦C. Mixing 10% of aqueous pomegranate peel extract (PPE) with 0.1 molar of zinc nitrate [Zn (NO3)2.6H2O], mixture was stirred at 70-80 ◦C for 15 minute and then left at room temperature overnight. The result was separated and centrifuged at 4000 rpm for 10 minutes. This process was repeated three times then the precipitate was collected and dried for using. ZnONPs were characterized by visual observation of color changes, estimate duration of synthesis compared to weight of ZnONPs was the result, UV-visible analysis, Fourier transform infrared (FT-IR), X- ray diffraction (XRD) and Scanning electron microscope (SEM). The results of this line, investigate the proper condition for ZnONPs synthesis. Result of FT-IR showed functional group related to Zn-O at (613.38 to 580.59 cm_1), whereas the result of X-RD at 2 theta diagnose the type of oxide formation as ZnO and determined particle size were found in range (20 - 30 nm). Finally, SEM image was shown crystals shape of ZnO nanoparticles. In conclusion, PPE acts as reducing agent used in synthesis of ZnO in Nano-scale size via easy and simple method.

Effects of pomegranate peel extract on quality and microbiota composition of bighead carp (Aristichthys nobilis) fillets during chilled storage

This study aimed to investigate effects of aqueous pomegranate peel extract (APPE) and ethanolic pomegranate peel extract (EPPE) on microbiota and changes in quality of bighead carp (Aristichthys nobilis) fillets stored at 4 °C. The results showed that pomegranate peel extract (PPE, which includes both APPE and EPPE) retarded the deterioration of sensory quality and flesh color, inhibited the growth of spoilage bacteria, and attenuated the production of biogenic amines, total volatile basic nitrogen (TVB-N), and the degradation of ATP-related compounds. Moreover, EPPE performed better in color attributes and biogenic amines, but APPE was more effective in retarding the increase of TVB-N and K-value. High-throughput sequencing results showed that microbial composition of all samples became less diverse as storage time increased. For the control group, Acinetobacter was predominant in the middle-period of storage, while Pseudomonas, Aeromonas, and Shewanella became predominant at the end of storage. Additionally, PPE decreased the relative abundance of Acinetobacter in the middle-period of storage, and thus changed the microbial composition. Based on our assessments of quality and microbial analysis, PPE prolonged the shelf-life of bighead carp fillets for about 2 days, and it has the potential to become a promising preservative in aquatic products.

Anticryptosporidial effect of pomegranate peels water extract in experimentally infected mice with special reference to some biochemical parameters and antioxidant activity.

Cryptosporidiosis is a zoonotic disease caused by a well-known parasitic protozoan called Cryptosporidium. Infection in livestock causes important economic losses among farm animals and its control has a global concern. In this study, internal white and external red layers were separated from pomegranate peels (Punica granatum) then; they were grinded to reach Nano form. Anticryptosporidial effect of their water extracts was investigated in experimentally infected mice. Also, their antioxidant activity, biochemical and histopathological changes were studied. Briefly, hot aqueous extracts of pomegranate peels were prepared regarding its good sensory attributes at concentration of 10% W/V. Analysis of total phenolics, individual phenolics by HPLC-DAD and antioxidant activities have been done. Forty-five mice were divided into five groups each one containing nine mice. The first group was healthy mice and the 2nd one was infected orally with 104 Cryptosporidium parvum (C. parvum)oocysts/mice and not treated. The other 3 groups were infected and orally treated with Nitazoxanide (NTZ) for the 3rd group, pomegranate red peel extract for the 4th group and pomegranate white peel extract for the 5th group. Blood samples were collected after 1 and 2weeks post treatment for protein profile, liver enzymes and antioxidant activity evaluation. After 3weeks, all animals were sacrificed and ileal tissues were embedded in paraffin for histopathological examination. The results showed that pomegranate peel extracts were rich in phenolic compounds, had high antioxidant activity and therapeutic effect on C. parvum in experimentally infected mice. Red peel extract diminished C. parvum oocysts count significantly in experimentally infected mice than white peel and NTZ treatments. Also, the histopathological examination revealed that red peel treated mice ileal sections showed a great enhancement in the shape and structure of villi towards normal structure than other treated groups. Most of the measured biochemical parameters after 2weeks' treatment with red pomegranate peel and NTZ were enhanced in their concentrations towards the healthy normal status. In conclusion, this study showed the effectiveness of Nano-form of pomegranate white and red peel extracts against C. parvum oocysts. Pomegranate red peel extract was found to have antioxidant activity that could significantly enhance the serum biochemical parameters and oxidative stress towards the healthy normal status. Furthermore, it is suggested that pomegranate peel should be separated and used in the daily animal diet oras a functional beavarage for human as accepted from the panelists to give protective effects against this parasite as well as to improve health benefits.

Evaluation of the antimicrobial efficacy of 20% Punica granatum, 0.2% chlorhexidine gluconate, and 2.5% sodium hypochlorite used alone or in combinations against Enterococcus faecalis: An in-vitro study.

Objectives:The study was aimed at evaluating the antimicrobial efficacy of 20% Punica granatum, 0.2% chlorhexidine (CHX) gluconate, and 2.5% sodium hypochlorite used alone or in combinations against Enterococcus faecalis.Materials and Methods:Aqueous extract of pomegranate peel was prepared in the Pharmacology Departmental Laboratory. A total of 240 wells were prepared (40 for each group) with 5 wells per with a diameter of 6 mm and depth of 4 mm at equidistant from each other. Using a pipette, each well was filled with 50 μl of the test irrigant solution. CHX (0.2%), 2.5% sodium hypochlorite, and aqueous extract of pomegranate peel and their combinations were tested as root canal irrigants against a standard strain of E. faecalis (ATCC 29212) on sheep blood agar plate by calculating the zones of inhibition. The mean diameter of zones was calculated and tabulated. Data were analyzed using one-way analysis of variance and Tukey's post hoc test. Descriptive statistics was obtained using SPSS software (version 11.5) with P established at < 0.05.Results:Combination of Punica granatum with sodium hypochlorite and CHX showed maximum mean zones of inhibition with mean of 23.9 and 25.7 mm, respectively, and showed significantly better results than all other groups either irrigants used alone or in combinations.Conclusions:Punica granatum and CHX was proved to be a very good combination among experimental groups against E. faecalis, and sodium hypochlorite was least effective against E. Faecalis.

Ultrasound assisted extraction of bioactive compounds from pomegranate (Punica granatum L.) peel

Ultrasound-assisted extraction (UAE) technology was used for extracting effective components from pomegranate (Punica granatum L.variety SisheKape-Ferdos) peel (POP). A response surface method was used to investigate the effects of independent process variables (ultrasonic amplitude (power control, UA): 20, 60 and 100%, and ultrasonic exposure time (UET): 5, 10 and 15 min) on the yield (Y) and antioxidant characteristics: total phenolic compounds (TPC), 1,1-diphenyl-2-picrylhydrazyl free radical scavenging (DPPH), ferric reducing-antioxidant power (FRAP), half maximal of radical-scavenging activity (IC50) of aqueous pomegranate peel extract. According to Derringer's desired function approach, the optimal conditions based on both individual and combinations of all process variables were UA 60% and UET 6.2 min, and at this optimum condition, the predicted maximum values of Y, TPC, DPPH, FRAP, and IC50 of the pomegranate peel extract (POPx) were 13.1%, 42.2 mg GA/g, 88.8%, 1824.6 μmol Fe2+/g, and 0.51 mg/mL, respectively. The experimental values were in a good agreement with the predicted values. Also, at optimal conditions, the minimum inhibitory concentration (MIC) of the extract (mean diameter of inhibition zone: 17.7 mm) showed that it had antifungal activities against Aspergillus niger.

Antidiarrheal effect of bioactivity-guided fractions and bioactive components of pomegranate (Punica granatum L.) peels.

Pomegranate peels have been widely used to treat diarrhea in China. The antidiarrheal activities of aqueous extracts of pomegranate peels have been evaluated. However, there have not been any bioactivity-guided fractionation studies on the antidiarrheal effect to identify the bioactive components of the extract. Bioactivity-guided fractionation of an aqueous extract of pomegranate peels was performed using different solvents of increasing polarity, generating fractions dissolved in ethyl acetate, n-butyl alcohol, and the residual fraction. The principal chemical composition of the active fraction was analyzed by HPLC/ESI-MS. Fecal frequencies revealed that only the ethyl acetate fraction possessed significant antidiarrheal activity. Furthermore, administration of the ethyl acetate fraction at 100, 200, and 400mg/kg significantly reduced gastrointestinal transit in charcoal meal tests in mice. It also significantly inhibited castor oil-induced enteropooling compared to control animals. Histopathological analysis revealed that small intestine lesions of mice treated with the ethyl acetate fraction were alleviated compared to those in mice treated with castor oil. The ethyl acetate fraction was found to be composed mainly of punicalagin, corilagin, and ellagic acid, and a combination of these compounds could mediate the antidiarrheal activities. Our study describes the protective effects of pomegranate peels against castor oil-induced diarrhea. The findings showed that the ethyl acetate fraction was the active fraction of pomegranate peels, of which punicalagin, corilagin, and ellagic acid were responsible for the antidiarrheal effect of aqueous extracts.

Preparation and characterization of biocompatible silver nanoparticles using pomegranate peel extract

The potential application of any nanoparticles, including silver nanoparticles (AgNPs), strongly depends on their stability against aggregation. In the current study, an aqueous extract of pomegranate peel was used as a stabilizer during synthesis of AgNPs. Nanoparticles have been prepared by the chemical reduction method from an aqueous solution of silver nitrate in the presence of sodium borohydride as a reducing agent. The AgNPs were characterized by dynamic light scattering (DLS), zeta-potential measurements, UV–Vis spectroscopy and transmission electron microscopy (TEM). The antibacterial efficiency of AgNPs against Escherichia coli was investigated. The size, polydispersity index, FWHM, and colloidal stability of nanoparticles in dispersion depends on the extract concentrations. In the presence of pomegranate peel extract, the nanoparticles suspension shows colloidal stability at least for a week. Our studies show that synthesized AgNPs with the above described procedure were stable at pH = 3–12 and in the temperature range of 25–85 °C. Additionally, AgNPs exhibit antibacterial properties, especially at the lowest amount of extract to silver ratio (KExtract/Ag).

The antimicrobial activity of alcoholic and aqueous extracts of pomegranate fruit peel on Staphylococcus aureus isolated from hair in Najaf

There has been considerable interest in the use of plant materials as an alternative method to control pathogenic microorganisms and many compounds of plant products have been shown to be specifically targeted against antibiotic resistant pathogens. The present study was aimed to evaluate the antimicrobial activity of the alcoholic and aqueous extract of pomegranate peel against Staphylococcus aureus isolated from hair. The plant extracts prepared and The antimicrobial effects of the pomegranate extracts were evaluated using method disk inhibition zone , the results shows that the extracts of pomegranate have a good antibacterial growth activity against Staphylococcus aureus isolated from hair with significant differences (P&lt;0.05) with concentrations of the type extract in which there are more susceptible to 100 mg/ ml( the highest concentration ) and the alcoholic extract.

Antioxidant activity of pomegranate peel extract on lipid and protein oxidation in beef meatballs during frozen storage

Antioxidant effect of pomegranate peel extract (PE) to retard lipid and protein oxidation in beef meatballs was investigated during frozen storage at −18±1°C. Concentrated and freeze dried aqueous extract of pomegranate peel was incorporated into freshly prepared meatball mix at 0.5% and 1.0% concentrations, and compared with 0.01% butylated hydroxytoluene (BHT) and control (without any antioxidant). In PE treated samples, particularly in high PE concentration, peroxide, malondialdehyde and carbonyl formation, loss of total protein solubility and sulfhydryl groups were significantly lower than control after 6months of storage. A diminution of both myofibrillar (MP) and sarcoplasmic (SP) proteins of high molecular weight was detected after 6months of the storage according to gel electrophoresis patterns. The 1.0% PE led to maintain colour intensity (C) and hue (h°) value. The results from sensory analyses revealed that PE addition to meatballs was effective on preventing rancid odour formation. Addition of both 0.5 and 1% PE in meatballs reduced lipid and protein oxidation and improved sensory scores. These results indicated that PE was effective on retarding lipid and protein oxidations.

The effect of pomegranate peel extract and vitamin C in comparison with gemifloxacin on inhibiting adhesion of Escherichia coli to uroepithelial cells

Background and objective: Prevention of bacterial adhesion is an attractive target for the development of new therapies in the prevention of bacterial infection. The aim of this study is to investigate the effects of pomegranate peel extract, vitamin C, combination of pomegranate peel extract and vitamin C &amp; gemifloxacin on adhesion of E.coli to uroepithelial cells. &nbsp; Methods: Uroepithelial cells were incubated with E. coli ATCC 25922 bacteria previously exposed to either the aqueous extract of pomegranate peel (AEPP),&nbsp;vitamin C, combination of both or gemifloxacin and the adherence was assessed by light microscopy. Results: AEPP showed good antibacterial activity with minimum inhibitory concentration MIC of 25μg/ml and 10 mmol/ml for vitamin C and upon combination, the MIC was10µg/ml &amp;5mmol/ml for AEPP &amp; vitamin C respectively whereas for gemifloxacin was 0.03μg/ml. In vitro and in vivo adhesion of E.coli to uroepithelial cells were significantly inhibited by AEPP, vitamin C alone and in combination and by gemifloxacin. Conclusion: AEPP showed good antibacterial activity, inhibited E.coli adhesion to uroepithelial cells and were potentiated by vitamin C.

ANTIOXIDANT AND ANTIMICROBIAL ACTIVITY OF POMEGRANATE (Punica granatum L.) FRUIT PEELS EXTRACT ON SOME CHEMICAL, MICROBIOLOGICAL AND ORGANOLEPTICAL PROPERTIES OF YOGHURT DURING STORAGE.

Pomegranate (Punica granatum) fruit peels was investigated for its phenolic compounds, antioxidant and antoxidative activity on L.A.B in yoghurt during cold storage . Results of chemical composition being 7.15 %, 1.22 % 0.65 % and 85.04 % for moisture, protein, crude fat and carbohydrates respectively. Total phenolic compounds were 42.62 mg gallic acid / g. Data of HPLC revealed that ten compounds were identified namely catechin , gallic and - chlorogenic acid being 138.71, 111.68 and 92.39% , Moreover catechol and caffein were 84.36 and 44.47 % and other trace compounds namely coumarin, cinnamic , chrysin, caffeic and ferulic . Radical scavenging activity DPPH for the aqueous extract was 90.97 %,compared with TBHQ 94.1% as synthetic one. Pomegranate peel extract explored considerable inhibitory effect of each of lactic acid bacteria, coliform group and Staph. Sp. The increase aqueous extract of pomegranate peel from 1to 5 % lead to an inhibitory effect on LAB and other tested pathogenic bacteria. 1 % of extract was appropriate for the viability of Lactic acid bacteria which was above 30 × 106 c.f.u ⁄ ml. In addition, using of 1 %, 0.75, 0.5 and 0.25% on yoghurt processing was acceptable for the chemical, microbiological and organoleptical properties of the resultant yoghurt. Moreover, the ratio of 0.75 % was more acceptable, comparing with other added doses.

Evaluation of Antibacterial Activity of Aqueous Extracts of Pomegranate Peels, Green Tea Leaves and Bay Leaves against Vibrio cholera

The antibacterial activity of aqueous extracts of Pomegranate peels, Green tea leaves, Bay leaves were evaluated in this study. Agar diffusion technique was applied. The three extracts were found to have inhibitory effect against Vibrio cholera and the mean of the diameter of inhibition zones ranges from (11-23 mm) for pomegranate peels, (9-20 mm) for Green tea leaves and (718mm) for Bay leaves extracts and statistically analyzed. There were significant differences between pomegranate peels, tea leaves and bay leaves extracts at each concentration used in this study. The pomegranate peels were found to have greater effects against Vibrio cholera followed by tea leaves, Bay leaves. Minimum bactericidal concentration (MBC) was also determined and it was shown that 10mg/ml was the MBC for pomegranate peels, 10-15mg/ml the MBC for tea leaves and 20-25mg/ml the MBC for Bay leaves.

Quality and antioxidant properties of a reduced-sugar pomegranate juice jelly with an aqueous extract of pomegranate peels

In the present study, the production of a reduced-sugar pomegranate juice jelly supplemented with an aqueous extract of pomegranate peel (PE) is described. Influence of different carbohydrate polymers (guar (G), xanthan (X) and tragacanth (T) gums) on rheological properties was studied. Combination GXT presented the most similar rheological behaviour to commercial jelly. Jelly (J) and jelly with PE (JE) were stored at 4°C over an 8week period for physical, chemical, antioxidant, microbiological and sensory analysis. J and JE showed similar values for °Brix, colour and Aw, though the pH of JE was lower than J. Thiol and phenolic compounds were higher in JE than in J. Antioxidant activity (radical scavenging activity and autoxidation of linoleic acid) was higher in JE than in J at 0weeks, and were decreasing with time. Pomegranate juice with additives was generally less accepted than J and JE.

Partial Identification of Antifungal Compounds from Punica granatum Peel Extracts

Aqueous extracts of pomegranate peels were assayed in vitro for their antifungal activity against six rot fungi that cause fruit and vegetable decay during storage. The growth rates of Alternaria alternata , Stemphylium botryosum , and Fusarium spp. were significantly inhibited by the extracts. The growth rates were negatively correlated with the levels of total polyphenolic compounds in the extract and particularly with punicalagins, the major ellagitannins in pomegranate peels. Ellagitannins were also found to be the main compounds in the bioactive fractions using bioautograms, and punicalagins were identified as the main bioactive compounds using chromatographic separation. These results suggest that ellagitannins, and more specifically punicalagins, which are the dominant compounds in pomegranate peels, may be used as a control agent of storage diseases and to reduce the use of synthetic fungicides.

Antibacterial activity of aqueous extract of pomegranate peel against Pseudomonas stutzeri isolated from poultry meat

In this study antibacterial activity of pomegranate peel (PPE) was evaluated against bacteria isolated from poultry meat. The bacteria were identified using 16S rRNA gene and DNA sequencing. Results of molecular characterization showed that the bacteria isolated were having 100% homology with the Pseudomonas stutzeri strain CTSP36 and further analysis showed that sample sequence clustered with the P. stutzeri strain CTSP36. Antibacterial activity of PPE was demonstrated by clear zone of inhibition in plates inoculated with extract. The diameter of inhibition zones were significantly (p < 0.05) higher in PPE as compared to standard antibiotic discs used (tetracycline, vancomycin and streptomycin). Results of broth dilution assay also revealed that PPE at 1%, 5% and 10% were effective in inhibiting bacterial growth in test plates. Further, a decrease in the growth of bacterial cells and a gradual decline in protein content of bacterial cells were also observed when bacterial culture was grown with different concentration of PPE along with a control. These results showed the potential application of pomegranate peel extract as antibacterial agent against P. stutzeri.