Abstract

Pomegranate (Punica granatum) fruit peels was investigated for its phenolic compounds, antioxidant and antoxidative activity on L.A.B in yoghurt during cold storage . Results of chemical composition being 7.15 %, 1.22 % 0.65 % and 85.04 % for moisture, protein, crude fat and carbohydrates respectively. Total phenolic compounds were 42.62 mg gallic acid / g. Data of HPLC revealed that ten compounds were identified namely catechin , gallic and - chlorogenic acid being 138.71, 111.68 and 92.39% , Moreover catechol and caffein were 84.36 and 44.47 % and other trace compounds namely coumarin, cinnamic , chrysin, caffeic and ferulic . Radical scavenging activity DPPH for the aqueous extract was 90.97 %,compared with TBHQ 94.1% as synthetic one. Pomegranate peel extract explored considerable inhibitory effect of each of lactic acid bacteria, coliform group and Staph. Sp. The increase aqueous extract of pomegranate peel from 1to 5 % lead to an inhibitory effect on LAB and other tested pathogenic bacteria. 1 % of extract was appropriate for the viability of Lactic acid bacteria which was above 30 × 106 c.f.u ⁄ ml. In addition, using of 1 %, 0.75, 0.5 and 0.25% on yoghurt processing was acceptable for the chemical, microbiological and organoleptical properties of the resultant yoghurt. Moreover, the ratio of 0.75 % was more acceptable, comparing with other added doses.

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