Antimicrobial and Cytotoxicity Effects of Synthesized Silver Nanoparticles from Punica granatum Peel Extract

Sandhanasamy Devanesan,Fadilah S Aleanizy,Akram A Alfuraydi,Amirtham Jacob A Ranjitsingh,Fulwah Y AlQahtani,Mohamad S AlSalhi,Radhakrishnan Vishnu Balaji,Ahmed H Othman,Anis Ahamed

doi:10.1186/s11671-018-2731-y

Sandhanasamy Devanesan, Fadilah S Aleanizy

Open Access

https://doi.org/10.1186/s11671-018-2731-y

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Journal: Nanoscale Research Letters	Publication Date: Oct 4, 2018
Citations: 108	License type: open-access

Affiliation: King Saud University

Abstract

To address the growing challenges from drug-resistant microbes and tumor incidence, approaches are being undertaken to phytosynthesize metal nanoparticles, particularly silver nanoparticles, to get remedial measure. In this study, an attempt has been made to utilize a major biowaste product, pomegranate fruit peel (Punica granatum), to synthesize silver nanoparticles. The silver nanoparticles (AgNPs) were synthesized using the aqueous extract of pomegranate peel. The formation of synthesized AgNPs was confirmed through UV-Vis spectroscopy, X-ray diffraction (XRD), transmission electron microscopy (TEM), scanning electron microscopy (SEM), and energy-dispersive X-ray spectroscopy (EDX) as well as through the change of the colorless aqueous solution to a dark brown solution. Using UV-Vis spectroscopy, the dark brown solution showed a Plasmon resonance band peak at 378 nm in UV-Vis spectroscopy after reacting for 24, 48, and 72 h. The XRD report revealed that the AgNPs had a cubic structure. The TEM and SEM report showed the nanoparticles were equally distributed in the solution, with a spherical shape and size ranging from 20 to 40 nm and with an average particle size of 26.95 nm. EDX imaging also confirmed the presence of AgNPs. The synthesized AgNPs were found to exhibit good antimicrobial effects on Gram-negative and Gram-positive bacteria, particularly the pathogens Escherichia coli (ATCC 25922), Pseudomonas aeruginosa (ATCC 27584), Proteus vulgaris (ATCC 8427), Salmonella typhi (ATCC 14028), Staphylococcus aureus (ATCC 29213), Staphylococcus epidermidis (MTCC 3615), and Klebsiella pneumonia. The cytotoxic effects of AgNPs were also tested against a colon cancer cell line (RKO: ATCC® CRL-2577™), and it was observed that the viabilities were 56% and 61% on days 3 and 5, respectively, with exposure to 12.5 μg of AgNPs. This simple, economic, and eco-friendly method suggests that the AgNPs biosynthesized using pomegranate peel extract may be a novel, potent solution for the development of a drug for colon cancer that also has antibacterial activity.

Highlights

In the last few decades, there has been a growing amount of research on nanotechnology, involving the green synthesis and characterization of nanoparticles, as nanoparticles less than 100 nm in size are ideal agents for drug delivery and biomedical applications [1]
The cytotoxic effects of AgNPs were tested against a colon cancer cell line (RKO: ATCC® CRL-2577TM), and it was observed that the viabilities were 56% and 61% on days 3 and 5, respectively, with exposure to 12.5 μg of AgNPs
This simple, economic, and eco-friendly method suggests that the AgNPs biosynthesized using pomegranate peel extract may be a novel, potent solution for the development of a drug for colon cancer that has antibacterial activity

Summary

Introduction

In the last few decades, there has been a growing amount of research on nanotechnology, involving the green synthesis and characterization of nanoparticles, as nanoparticles less than 100 nm in size are ideal agents for drug delivery and biomedical applications [1]. Devanesan et al Nanoscale Research Letters (2018) 13:315 to formulate nanoparticles that can interact with target biomolecules [6]. This approach to the synthesis of silver nanoparticles may play an important role in future treatments for various forms of cancer or other ailments that can be controlled by phyto-nanotechnology [7, 8]. Surgical infections, including pneumonia and bloodstream infections, are due to the presence of Gram-positive and Gram-negative bacteria [10]. Plant-mediated synthesis of AgNPs can help in the development of effective antibacterial agents against microbial pathogens of public health relevance. Many researchers have reported that synthesized AgNPs contain well-known antimicrobial properties against Gram-positive and Gram-negative pathogens, as well as cytotoxic effects on different cancerous and normal cell lines [12–14]. AgNPs are highly efficient due to a high-surface-area-to-volume ratio, can disrupt, and have the ability to penetrate bacterial cells when compared to silver ions alone [13]

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