Aqueous Humor Of Eyes Research Articles

The alpha and gamma crystallin content in aqueous humor of eyes with clear lenses and with cataracts

Specific radioimmunoassays were used to measure the concentration of α- and γ-crystallins in human aqueous humor. It was demonstrated that these crystallins are normally present in aqueous humor from healthy eyes. The crystallin concentration did not seem to increase with age. The normal upper limit for the α-crystallin concentration was found to be 10 ng/ml and for the γ-crystallin concentration 60 ng/ml. In the aqueous humor of eyes with cortical cataract the concentration of both crystallins was increased. With nuclear cataracts the α-crystallin concentration was increased while the γ-crystallin concentration was decreased. Experiments in rabbits showed that the crystallins in the aqueous humor left the anterior chamber at the same rate as the aqueous bulk flow. The demonstration of lens crystallins in the aqueous humor is compatible with the hypothesis that they leak from the lens.

Identification of a fluorescein tracer model for determination of the flow rate of aqueous humor in the eye

The continuous flow of the aqueous humor through the posterior and anterior chambers in the eye into Schlemm's canal is vital for the maintenance of a constant intraocular pressure. If the intraocular pressure rises above the normal range, several problems start appearing insidiously; for example, gradual visual field defects develop, leading ultimately to glaucoma. The determination of the flow rate of the aqueous humor is here performed by identifying a mathematical model after the free fluorescein concentration in the blood and in the anterior chamber of the eye has been measured. Experimental data obtained on rabbits are used in the analysis, and the flow rate of the aqueous humor in rabbit eyes is computed. This study demonstrates the feasibility of the approach in a clinical environment.

Lactic dehydrogenase activity in the aqueous humour of eyes containing malignant melanomas.

Lactic dehydrogenase activity in the aqueous humour of eyes containing malignant melanomas.

Gentamicin Penetration in the Aqueous Humor of Eyes with Corneal Ulcers

Gentamicin Penetration in the Aqueous Humor of Eyes with Corneal Ulcers

The fluorescent antibody tissue stain in experimental ocular syphilis.

The fluorescent antibody tissue stain for treponemes is described and its usefulness in experimental ocular syphilis documented. Two primates, inoculated by intraocular and intracisternal routes, respectively, acquired syphilis serologically but only one developed clinical lesions. The fluorescent antibody tissue stain (FA) demonstrated the presence of treponemes in aqueous humor from each animal. It was of interest that<i>Treponema pallidum</i>could be demonstrated in the aqueous humor of eyes that were virtually normal at the time of paracentesis. Further investigation of the FA stain in the diagnosis of syphilis is suggested.

A Light-catalysed Reaction in the Aqueous Humour of the Eye

Kon and Watson1 found that ascorbic acid in milk was destroyed by light and Hopkins2 showed that this destruction was caused by the photocatalytic oxidation of ascorbic acid by riboflavin. Oxygen was necessary and peroxide was formed. In connexion with an examination of glutathione peroxidase of lens I became interested in the ascorbic acid of the aqueous humour as a possible source of hydrogen peroxide. In many mammals, including man, the level of ascorbic acid in the aqueous humour is about 1.0 mM, which is far higher than the level in serum3. Light freely penetrates through the aqueous humour whenever the eye is open, and Philpot and Pirie4 found traces of riboflavin and/or flavinadenine dinucleotide in the aqueous humour of cattle eyes. It seemed possible, therefore, that a photocatalysed oxidation of ascorbic acid might take place in the eye in vivo, as it does in milk in vitro.

Changes in the adrenalin content of the blood and of the aqueous humor of the eye of the rabbit after irradiation

The change of the content of adrenalin was studied in the blood as well as in the aqueous humor of the rabbit's eye after total irradiation with the dose of 800 r. The polarographic method was used for the quantitative determination of the adrenalin content. After total irradiation of the rabbit distinct and regular changes of phasic character take place in the content of adrenalin in the blood and in the aqueous humor of the eye.