Abstract Nanocomposite films were obtained by solvent casting method from aqueous solutions of furcellaran (FUR) and gelatin (GEL) with addition of various concentrations of nanoparticles (Se-AgNPs). The incorporation of Se-AgNPs gave rise to changes in the physical and mechanical properties of the nanocomposite films. The increase in the concentration of Se-AgNPs results in change of elasticity and elongation at break. The films with Se-AgNPs possess strong antibacterial activity against Staphylococcus aureus, Multi Resistant Staphylococcus aureus (MRSA) and Escherichia coli. This work aims to study the effect of nanocomposite films on quality of kiwi (Actinidia arguta) during storage period. Fresh kiwis in nanocomposite films had longer shelf lives than fruits in LDPE film. It follows from the study that the films are suitable for storage of kiwis.