Apulia Region Research Articles

How we can improve the appropriateness of ambulatory gastrointestinal endoscopy: the lesson of the COVID-19 outbreak.

The coronavirus disease 2019 (COVID-19) outbreak has significantly modified hospital and outpatient activities. To assess the pandemic's impact on the appropriateness of outpatient gastrointestinal (G.I.) endoscopy in a southern Italy Region. Between 1 January 2019 and 31 December 2022, we compared the number, the type of activities, and the appropriateness of the G.I. endoscopy procedures in the four territorial gastroenterology and digestive endoscopy services of the Apulia region. In 2019, 11 173 outpatient procedures were provided, which dropped by about 44% (P < 0.05) during 2020. The endoscopic activity was slightly reduced during 2020 (-18.77%), while every other activity dropped significantly. During 2021-2022, the overall activities slightly increased but remained below the number of activities provided before the pandemic (P < 0.05). However, the endoscopic activity increased by +6.86% from 2019 to 2022, mainly due to more colonoscopies. During the 4 years analysed, the overall appropriateness rate of the endoscopic examinations increased during 2020 (2019: 67%; 2020: 81%) while dropping again in the following years (2021: 75%; 2022; 69%). The appropriateness of the G.I. endoscopic procedures in the territorial gastroenterology services in the Apulia Region has improved during the acute phase of the COVID-19 pandemic, dropping again in the following years. Overall, the appropriateness of endoscopic procedures remains suboptimal; thus, educational activities are needed to improve the appropriateness rate to optimise the limited resources.

Towards sustainable marine spatial planning of aquaculture

An efficient and adaptive strategy within the EU Marine Spatial Planning Directive has to manage the existing and increasing conflicts between human uses and habitat conservation in coastal-marine areas. Among the different human activities developed along the coasts, aquaculture occupies a primary role. In this context, the aims of this research have been: (1) to propose a conceptual model suitable for aquaculture marine spatial planning; (2) to collect and integrate indicators useful for the characterization of the study area in terms of socio-ecological-economic sensitivities and pressures; and (3) to identify and map the most suitable areas for the development of new fish and shellfish farms. The study area is the Apulia Region (Southern Italy) with a coastline of about 1,000 km, in the Adriatic and Ionian Seas, and characterized by several economic activities within a high value natural context. The evaluation of area’s suitability for fish and shellfish farms have been carried out through the ecological characterization of the coastal areas, the identification of the socio-economic, ecological, cultural, and legal-military constraints and the estimate of the “Suitability Index” that, through a Weighted Linear Combination, integrates environmental variables and allows to classify areas as “Highly Suitable”, “Suitable” or “Unsuitable”. The “highly suitable areas” for new fish and shellfish farms are mainly located in northernmost of the Southern Adriatic Sea along the Gargano coast and in the Gulf of Manfredonia, whilst concerning the Northern Ionian Sea they are at a mean distance of 5 Km from the shoreline. The suitability maps have shown that existing fish farms are in line with their suitable areas but, surprisingly, this has seemed not to be true for shellfish farms. This can be explained by the fact that these aquaculture activities have traditionally been present in some areas (e.g., Taranto Seas), currently strongly impacted by human activities. This research has highlighted that despite aquaculture is generally conceived as an environmental impacting activity, it could be also impacted by other environmental and/or anthropic stressors (i.e., industrial ports, sewage discharges). Considering all these elements, the present research addresses decision-makers, providing information and tools necessary to plan in a more aware way, and also stakeholders interested in investing in the aquaculture sector, who could benefit from the proposed suitability maps for fish and shellfish farms for a sustainable development of this sector.

3D MODELS CITYGML-BASED COMBINED WITH TECHNICAL DECISION SUPPORT SYSTEM FOR THE SETTING UP OF DIGITAL CONSERVATION PLANS OF HISTORIC DISTRICTS

Abstract. The setting up of recovery plans for historic districts requires a multi-level and multi-thematic process for their analysis and diagnosis to determine classes of priorities and interventions for buildings at the district scale of relevance. Traditional tools and protocols have already highlighted operative complexity and expensive activities, affecting the organicity and effectiveness in interpreting data. On the other hand, recent scientific and practical activities based on the use of parametric Digital Models and Informative Systems have highlighted their advantages in standardizing complex issues and knowledge. Recent work by the authors has defined the structured organization of technical knowledge for the creation of a digital recovery plan using Informative Parametric Models, based on descriptors, and primary and secondary factors. These aim at converting properties and information in qualitative and quantitative data, and then structuring dependencies on descriptors and primary factors, according to thematic taxonomies, existent ontologies for the geometric and semantic representation of urban and architectural entities, thematic normative and established approaches for the recovery of cultural and landscape heritage. Thus, the present work shows a workflow for the semi-automatic setting up of intervention classes for architectures in historic districts in Italy. It is structured on CityGML-based models, coherently implemented with a Technical Decision-Support System (T-DSS). Specifically, the T-DSS is determined considering the relations among thematic normative: UNI 11182, UNI/CEN TS 17385:2019, and Italian Consolidated Law on Building. The workflow is finally tested in the historic district of Ascoli Satriano, in the Apulia Region (South of Italy).

Environmental and economic benefits of wheat and chickpea crop rotation in the Mediterranean region of Apulia (Italy)

Wheat plays an essential role in safeguarding global food security. However, its intensive agricultural production, aimed at maximizing crop yields and associated economic benefits, jeopardizes many ecosystem services and the economic stability of farmers. Rotations with leguminous are recognized as a promising strategy in favor of sustainable agriculture. However, not all crop rotations are suitable for promoting sustainability and their implications on agricultural soil and crop quality should be carefully analyzed. This research aims to demonstrate the environmental and economic benefits of introducing chickpea into a wheat-based system under Mediterranean pedo-climatic conditions. For this purpose, the crop rotation “wheat-chickpea” was evaluated and compared with the conventional regime (wheat monoculture) by means of life cycle assessment methodology. For this purpose, inventory data (e.g., agrochemical doses, machinery, energy consumption, production yield, among others) was compiled for each crop and cropping system, thus converted into environmental impacts based on two functional units: 1 ha per year and one € of gross margin. Eleven environmental indicators were analyzed, including soil quality and biodiversity loss. Results indicate that chickpea-wheat rotation system offers lower environmental impacts, regardless of the functional unit considered. Global warming (18 %) and freshwater ecotoxicity (20 %) were the categories with the largest reductions. Furthermore, a remarkable increase (96 %) in gross margin was observed with the rotation system, due to the low cost of chickpea cultivation and its higher market price. Nevertheless, proper fertilizer management remains essential to fully attain the environmental benefits of crop rotation with legumes.

Detection by Sensitive Real-Time Reverse Transcription Loop-Mediated Isothermal Amplification of Olive Leaf Yellowing Associated Virus and Its Incidence in Italy and Spain

Olive trees (Olea europea L.) are constantly threatened by many viruses, such as the olive leaf yellowing-associated virus (OLYaV), that belong to the Olivavirus genus, family Closteroviridae. In this work, the OLYaV incidence in different regions of Italy and Spain, which represent the two most important European areas for olive production, was evaluated through the development of a real-time reverse transcription-loop-mediated isothermal amplification (RT-LAMP) for reliable and sensitive OLYaV detection. The specificity and accuracy of the developed real-time RT-LAMP assay were determined; the assay showed that potential cross-reactivity with other viruses belonging to the Closteroviridae family was excluded. The LAMP assay detected OLYaV with a higher sensitivity than conventional end-point RT-PCR, detecting a total of 1.34 × 10−2 genome copies. A total of 80 and 120 plants of different olive cultivars from Spain (Comunitat Valenciana, Andalusia) and Italy (Sicily, Calabria, Apulia, Lazio, and Umbria) regions were tested, respectively. The percentage of infected plants was 46.25% and 30% for Spain and Italy, respectively, while the most susceptible cultivars were “Serrana Espadán” and “Villalonga” from Comunitat Valenciana and Andalusia regions (Spain) and “Ogliarola barese” from Apulia region (Italy). In addition, the survey demonstrated that the real-time RT-LAMP showed good sensitivity for OLYaV-positive sample detection, especially on asymptomatic olive trees. For this reason, the developed assay could be very suitable for phytopathological laboratories as a reliable and efficient method for a rapid and sensitive routine test on olive samples.

#5104 GLOMERULAR DISEASES ASSOCIATED WITH SARS-COV2 INFECTION AND VACCINATION: A MULTICENTRIC REGIONAL RETROSPECTIVE STUDY

Abstract Background and Aims The SARS-CoV2 caused a pandemic disease, leading to millions of cases and fatalities worldwide. Kidney involvement is frequent and glomerular diseases (GD) have been reported in association with infection. Podocytopathies, endothelial injury with thrombotic microangiopathy are the most common histological findings in literature. From January 2021 anti SARS-CoV2 vaccination became available. Several GD has also been associated with vaccination. IgA nephropathy (IgAN) and minimal change disease (MCD) are the most common GD reported in patients who received vaccination. Our aim was to assess the histological findings in patients who presented kidney involvement after SARS-CoV2 infection and/or vaccination and to compare the frequencies of GD in these patients to those in non-Covid19 population. Method We conducted a retrospective study of kidney pathology in patients undergoing native kidney biopsy within 90 days of SARS-CoV2 infection and/or vaccination, between February 2020 and August 2022 in Apulia Region of Italy. We considered separately patients without history of urinary abnormalities and/or kidney dysfunction prior to infection and/or vaccination in order to detect GD which were more likely to be related to infection and/or vaccination than to pre-existent pathologies. We used a database of kidney biopsies performed in a period of 5 years (from January 2015 to February 2020) prior to the Covid19 pandemic as a control comparison cohort to examine glomerular diseases frequencies. Results Among the 808 biopsies: 36 (4.4%) were carried out within 90 days of SARS-CoV2 infection (Group 1) and 142 (17.6%) within 90 days of vaccination (Group2). Among them, we identified 12 patients with renal symptoms raised after COVID19 infection (Group 3) and 26 patients after vaccination (Group 4). In the Group 1 the main histological diagnosed founded were: podocytopathies (25.1%), IgAN (16.7%) and ANCA-vasculitis (13.9%). In Group 2, the most common histological diagnosis were IgAN (18.3%), podocytopathies (17%) and membranous nephropathy (MN) (10.6%). In the Group 3 we found a predominance of podocytopathies (33.3%) followed by ANCA-vasculitis and IgAN (three patients each, 25%). In the Group 4, the most frequent GD were podocytopathies (36.6%) followed by ANCA-vasculitis (19.2%) and IgAN (15.4%). Between the 1380 kidney biopsies from the 5 years pre-Covid19 period, the most frequent histological diagnosis were IgAN (18.3%), podocytopathies (18%) and MN (10.9%). Comparing Group 1 and Group 2 data with the 5 years pre-Covid19 database we found a significant increased prevalence only of ANCA vasculitis in group 1 (13.9% of patients after Covid19 infection vs 5.6% of pre-covid database (p-value: 0.03)). Comparing data from patients who had developed renal signs after Covid19 infection (group 3) and/or vaccination (group 4) with those in the 5 years database a significant higher prevalence of ANCA-related crescentic glomerulonephritis was found in both group. The 25% of patients of the Group 3 and the 19.2% of the Group 4 had a histological diagnosis of vasculitis, while these were found only in the 5.7% of patients from the pre-Covid19 database (p-value: 0.004 for Group 3 and 0.003 for Group 4). Furthermore podocytopathies in group 4 were significantly higher than pre-covid19 (36.6%vs18.3%, p = 0.02). Conclusion We found a higher prevalence of ANCA vasculitis and podocytopathies in patients with symptoms developed after SARS CoV2 infection/immunization compared to diagnosis prevalence in a pre-Covid19 reference database.

Preliminary survey on the occurrence of microplastics in bivalve mollusks marketed in Apulian fish markets

Microplastics (MPs) are a relevant threat to food safety because they are ingested by humans through various foods. Bivalves are at high risk of microplastic contamination due to their filter-feeding mechanism and pose a risk to consumers as they are ingested whole. In this work, microplastics were detected, quantified, identified, and classified in samples of mussels (Mytilus galloprovincialis) and oysters (Crassostrea gigas) marketed in the Apulia region. The total number of plastic debris was 789 particles in the mussel samples and 270 particles in the oyster samples, with size ranging from 10 to 7350 µm. Fragments with size within the category of 5-500 µm were the predominant findings in both species, with blue as the predominant color in mussels and transparent in oysters; most of the debris was polyamide and nylon polymers in the mussels and chlorinated polypropylene in the oysters. These results show that mussel and oyster samples purchased at fish markets are contaminated with microplastics. The sources may be diverse and further studies are needed to assess the impact of the marketing stage on microplastic contamination in bivalves to better define the human risk assessment associated with microplastic exposure from bivalves consumption.

Sustainable strategies of industrial tourism in the agri-food business: an exploratory approach

PurposeThis study aims to analyze the opportunities and potential of industrial tourism for the agri-food industry in the current post-pandemic scenario. Industrial tourism can favor appropriate behaviors by companies and tourists to address emerging challenges in the post-pandemic era, such as responsible production and consumption and the containment of waste production.Design/methodology/approachThe adopted research method is a case study with exploratory purposes, applied to an industrial tourism experiment called “Make it in Puglia” (Apulia Region, Italy). The case study was based on participant observation and semi-structured interviews.FindingsThis study analyzes how industrial tourism in active agri-businesses allows local areas to be enhanced while simultaneously improving and strengthening sustainable strategies and choices for organizations and customers, especially in the changing context of the post-pandemic era.Research limitations/implicationsThe country specificity of this project and the limited duration of the investigation in the post-pandemic period were the main limitations of this study.Practical implicationsIndustrial tourism in the agri-food business can contribute to a sustainable strategy for destination management based on new elements of attractiveness. Moreover, industrial tourism represents an opportunity for agri-food businesses, through the promotion of “business tours,” to respond to requests for more sustainable practices, especially considering that food waste and resource use efficiency have become a topic of growing concern.Originality/valueThis paper is one of the first studies that considers industrial tourism as a strategy for achieving sustainable development goals (SDGs) in the agribusiness sector, as it analyzes industrial tourism as an instrument in the construction of a cohesive ecosystem where tourism, agri-food business and local communities together pursue preservation and safeguarding of the environment, heritage and territory.

Hospitalization for bronchiolitis in children aged ≤ 1year, Southern Italy, year 2021: need for new preventive strategies?

BackgroundBronchiolitis is a major cause of hospitalization in infants, particularly in the first six months of life, with approximately 60–80% of admissions due to respiratory syncytial virus (RSV) infection. Currently, no prophylactic options are available for healthy infants. The present study aimed at describing the demographic, clinical, and epidemiological characteristics of infants hospitalized for bronchiolitis in the Apulia region of Italy in 2021.MethodsFrom January to December 2021, data on children aged 0–12 months admitted for bronchiolitis in nine neonatal or pediatric units covering 61% of pediatric beds of hospitals in the Apulia region of Italy were analyzed. Demographic data, comorbidities, need for oxygen support, length of hospital stay, palivizumab administration, and outcomes were collected. For the purpose of the analysis, patients were divided into those aged 0–3 months and > 3 months. A multivariate logistic regression model was used to explore associations between the need for oxygen support and sex, age, comorbidities, history of prematurity, length of hospital stay, and palivizumab administration.ResultsThis study included 349 children aged 0–12 months admitted for bronchiolitis, with a peak of hospitalization in November (7.4 cases/1,000 children). Of these patients, 70.5% were RSV positive, 80.2% were aged 0–3 months, and 73.1% required oxygen support. Moreover, 34.9% required observation in the sub-intensive care unit, and 12.9% in the intensive care unit. Of the infants who required intensive care, 96.9% were aged 0–3 months and 78.8% were born at term. Three patients required mechanical ventilation and one, who required Extra Corporeal Membrane Oxygenation, died. Children aged 0–3 months were more likely to show dyspnea, need oxygen support, and have a longer hospital stay.ConclusionsThe present study showed that almost all of the children who required intensive care support were aged ≤ 3 months and most were born at term. Therefore, this age group remains the highest risk group for severe bronchiolitis. Preventive measures such as single-dose monoclonal antibody immunoprophylaxis, and maternal and childhood vaccination against RSV, may reduce the high public health burden of bronchiolitis.

On the energy performance of the Mediterranean school building stock: The case of the Apulia Region

The refurbishment opportunities provided by climate policies require an adequate knowledge of the school building stock, characterised by an urgent need of maintenance. Nevertheless, empirical evidence on energy performance of school samples appears limited due to the difficulty in retrieving data, although field data analysis is crucial in the built environment management. This study aims to explore existing energy conditions of an educational building sample hosting pre-schools, primary and lower secondary schools, located in southern Italy (Apulia Region). Firstly, an overview of the schools based on data retrieved from the regional dataset was performed. Then, more than 1000 buildings were clustered based on two predictors (construction year and surface-to-volume ratio), identifying five clusters representing the majority Apulian schools. In addition, billed gas and electricity data collected for 47 schools over a five-year period (2017–2021) were analysed, identifying annual and monthly trends, benchmarks, and mean values, which account for 46.5 (gas consumption), 15.59 kWh/m2 (electricity consumption). On average, source total consumption in 2020 experienced a reduction of 20%, partly due to Covid-19 restrictive measures. Finally, factors affecting heating consumptions were explored, and a regression analysis was performed, identifying heating degree days, construction year and boiler power to be the most significant.

Variability in Productive and Biochemical Traits of Vicia faba L. Landraces from Apulia Region (South Italy)

The faba bean (Vicia faba L. var. major) is a pulse that is garnering attention for its chemical composition, which makes it suitable for a healthy diet. The Apulian germplasm is rich in local accessions at risk of genetic erosion, which need evaluating and promoting. Thirteen Vicia faba local Landraces have been analyzed in relation to their productivity and their chemical and biochemical characteristics: their protein, total phenol, total flavonoid, condensate tannin and L-DOPA levels. The results showed great variability—above all in the thousand-seeds weight and in their content of proteins and L-DOPA. Among the accessions evaluated, the two collected from the most southern area of the region (FV12-FV10) were particularly promising—both for their good biochemical traits and, especially, for the higher L-DOPA content (0.46 and 0.49 g 100 g−1 d.m., respectively), even when expressed in terms of yield per plant (116.3 and 153.0 mg plant−1 d.m., respectively).

The Habitat Network for Butterfly Communities of the Alta Murgia National Park (Apulia, Italy)

Habitat networks can help to make habitats more resilient and assist species survival in a fragmented landscape and changing climate. Butterflies are one of the main indicators of diversity due to their high sensitivity to environmental changes. In the context of sudden and unpredictable environmental changes, protection strategies for butterflies at risk of extinction should consider the exact distribution of these species, as well as the various threats to which each of them is subjected. About 290 species of butterflies are reported in Italian fauna, and 120 of them are recorded in Apulia (Southern Italy). In the Alta Murgia National Park (AMNP) (Apulia Region, Italy), screening was performed to study the relationships between area/landscape composition and diurnal butterfly community structure. Representative semi-natural habitats of Alta Murgia buffering productive crops were selected to set up transects/paths along dry grasslands, oak forests and pine forests. Monthly samplings were performed for one year. During the survey, 909 specimens from 53 species were collected. The highest values of butterfly’s abundance and richness were recorded in dry grasslands. A strong positive correlation between butterfly abundance and air temperature was registered (Pearson correlation: r = 0.8; p &lt; 0.001). Melanargia arge, endemic in central-southern Italy and considered threatened in Europe (Annexes II and IV—Habitats Directive), was registered in each habitat. The biodiversity indices (Chao 1, Shannon and Simpson) for each habitat were high and similar to those in protected areas of Sicily. The connected landscape is important for generalist or open-habitat specialists, and large remnants are key for disturbance-sensitive and threatened taxa. The presented evidence can provide useful information on butterfly conservation in the AMNP and for the management and conservation of characteristic landscapes of Alta Murgia.

1H-NMR Metabolomics Study after Foliar and Endo-Therapy Treatments of Xylella fastidiosa subsp. pauca Infected Olive Trees: Medium Time Monitoring of Field Experiments.

Here we report the medium-term effects of foliar spray and endo-therapy treatments with different doses of a Cu/Zn citric acid biocomplex (Dentamet®) in Xylella fastidiosa infected olive trees of Salento, Apulia region (South-east Italy). Leaf extract samples from field-treated 150 years old olive trees cvs Ogliarola salentina and Cellina di Nardò were studied by 1H NMR-based metabolomics. The result of different applications of Dentamet® endo-therapy after 60, 120 and 180 days in comparison with traditional foliar spray treatment and water injection as a control have been investigated. The metabolic profile analyses, performed by 1H NMR-based metabolomic approach, indicated plant metabolites variations connected to the disease progression such as mannitol, quinic acid, and oleuropein related compounds. The best results, in terms of discrimination of the metabolic profiles with respect to water injection, were found for monthly endo-therapy treatments. Dentamet® foliar application demonstrated more specific time related progressive effectiveness with respect to intravascular treatments. Therefore, besides a possible more effective performance of endo-therapy with respect to foliar treatments, the need of further doses/frequencies trimming to obtain long-term results was also assessed. The present field studies confirmed the indication of Dentamet® effectiveness in metabolic variation induction, potentially linked with reducing the X. fastidiosa subspecies pauca related Olive Quick Decline Syndrome (OQDS) symptoms development.

Field management of Phelipanche ramosa on tomatoes by plowing depth or resistance strategies

Two separate independent experiments were carried out on agronomic approaches for controlling infestation by Phelipanche ramosa: the evaluation of two soil plowing depths (30 and 50 cm) and determining the resistance of two tomato cultivars (SV5197 and SV8840) to this parasitic plant. The experiments were performed in 2014 and 2018 seasons in naturally heavily infested fields in the province of Foggia (Apulia Region, south-eastern Italy). Based on our results, the 50 cm moldboard plowing depth reduced parasite infestation by 41.2% compared to that of 30 cm depth, commonly adopted by local farmers. Higher attachments to the host plants were observed in SV5197 than in SV8840, which could be correlated with the different level of resistance of the host plant. In both experiments, the highest Phelipanche infestation reduced the tomato yield but did not affect the fruit quality.

Participatory Planning for the Drafting of a Regional Law on the Bioeconomy

In an increasingly complex global economic scenario, sustainability represents a fundamental compass aimed to guide actions of institutions and individuals. A nondissipative use of Earth’s resources is feasible through a common effort that reconsiders the actual development system according to the key principles of the bioeconomy. It is vital to start from local contexts to reach the global dimension by exploiting the opportunities available in each territory. Starting from these assumptions, the participatory process activated in the Apulia region has represented the first step towards an intervention strategy in the panorama of the bioeconomy, and has made it possible to increase the awareness of a development based on the adoption of bioeconomy models and, therefore, circular economy ones through an effective inclusion process. A process has given rise to a project allowing all involved actors to reflect on the double economy–environment system, to share good practices and promote the adoption of lifestyles and consumption styles more compatible with the principles of the bioeconomy and to elaborate a proposal for a participatory regional law for the bioeconomy in the Apulia region as an expression of the collaboration between different bodies and institutions (universities, Confindustria and the council of the Puglia region).

Environmental surveillance of Sars-Cov-2 in wastewater in Apulia region, Southern Italy

Environmental surveillance of Sars-Cov-2 in wastewater in Apulia region, Southern Italy

Incidence and Prevalence of Multisystem Inflammatory Syndrome in Children (MIS-C) in Southern Italy.

Multisystem inflammatory syndrome in children (MIS-C) is a pediatric hyperinflammatory syndrome related to severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection whose epidemiology is not very well known at present. The objective of the study was to better understand the incidence of MIS-C in the Apulia region in southern Italy. Our primary goal was to estimate the incidence of newly identified cases of MIS-C in children aged 0-18 years, during a period of six months, encompassing the second pandemic wave. We also analyzed the characteristics of our cohort in terms of clinical features, treatment, and outcomes. The cumulative incidence of MIS-C was 3.27 per 100,000 residents between 0 and 18 years of age. In our cohort, gastrointestinal, mucocutaneous, and cardiac involvement were the most common clinical features. With our step-up approach to therapy, no patients required intensive care unit (ICU) admission and no cardiac sequelae after 6 months of onset were found in echocardiograms. Conclusion: Our epidemiological study of MIS-C in southern Italy showed unexpectedly overlapping figures with other US studies.

Stakeholder Empowerment in Sustainable Rural Development Partnerships: Two Case Studies from Italy

The funds allocated through the EU Rural Development Programme provided the engine for local stakeholders to interact, forming mixed collaboration partnerships. This paper investigates the structure of such partnerships with the aim of verifying whether (R1) there are significant differences between the various stakeholder categories in influencing the rural development process, and (R2) which categories of stakeholders are more empowered in directing the design of sustainable rural development. The study is focused on two Italian cases: the regions of Apulia and Veneto. Using a combination of SNA and nonparametric tests, the results demonstrate that the empowerment of the stakeholders followed unequal paths in the two cases; i.e., a central role is clearly played by economic associations in Apulia, while this power is more distributed between different kinds of stakeholders in the case of Veneto. Agricultural associations in Apulia play an important role in the densely connected rural development network, promoting information flow and collective action. On the other hand, the weakness of this configuration lies in the fact that the rural development agenda can receive strong pressure from the agricultural sector, pushing more sectoral strategies in turn. Private companies play a key role in Veneto’s rural development, bridging the network gaps between more clustered local groups and increasing pluralism and inclusion. However, the network is sparse and shrinking, posing challenges in terms of coordination and collective action. This kind of evaluation makes policymakers and managers aware of both the most influential and weakest actors. This is crucial to improving the effectiveness and sustainability of the project, as they can involve the most influential groups from the early stages of the design process to ensure support as well as address the needs of the lagging stakeholder categories to reinforce tacit rules, trust, accountability, and responsibilities.

REGIONAL OBSERVATORY OF PHYSICAL FITNESS IN CHILDREN AND ADOLESCENTS IN SOUTHERN ITALY: A THIRTY-YEAR COMPARISON BETWEEN 1990-2020

International literature showed that lower physical activity levels are associated with a progressive decline in physical fitness levels in children and adolescents over the past decades. The present cross-sectional study aims to assess trends in physical fitness levels among young adolescents in Lecce (Apulia region, Italy) comparing motor performances in 1990 and 2020. The sample consisted of 107 children attending the secondary school in Lecce in 1990, and 118 children attending secondary school in the same province in 2020, divided according to gender and BMI cut-off (normal weight and overweight/obese). Physical fitness assessment included standing long jump, medicine ball throw 2kg, 30-m dash and Cooper endurance running proposed both in 1990 and 2020. The analysis of variance (ANOVA) was performed to compare motor performances (1990 vs 2020) according to gender and BMI, and on total sample according to BMI; chi-square test was carried out to underline differences between Normal Weight, Overweight and Obese distribution in 1990 and 2020, and linear regression analysis was executed to investigate the variance in motor test explained by BMI, both in 1990 and 2020 groups. Results showed that: (a) sample in 1990 performed significantly better than those in 2020 in all of the above physical fitness test, independently for gender and BMI cut-off, (b) the percentage of overweight obese children has increased significantly from 1990 to 2020, and (c) BMI is a key factor influencing almost all motor performance in 2020.

High Genetic Diversity and Virulence Potential in Bacillus cereus sensu lato Isolated from Milk and Cheeses in Apulia Region, Southern Italy.

The Bacillus cereus group includes species that act as food-borne pathogens causing diarrheal and emetic symptoms. They are widely distributed and can be found in various foods. In this study, out of 550 samples of milk and cheeses, 139 (25.3%) were found to be contaminated by B. cereus sensu lato (s.l.). One isolate per positive sample was characterized by Multilocus Sequence Typing (MLST) and for the presence of ten virulence genes. Based on MLST, all isolates were classified into 73 different sequence types (STs), of which 12 isolates were assigned to new STs. Virulence genes detection revealed that 90% and 61% of the isolates harboured the nheABC and the hblCDA gene cluster, respectively. Ninety-four percent of the isolates harboured the enterotoxin genes entS and entFM; 8% of the isolates possessed the ces gene. Thirty-eight different genetic profiles were identified, suggesting a high genetic diversity. Our study clearly shows the widespread diffusion of potentially toxigenic isolates of B. cereus s.l. in milk and cheeses in the Apulia region highlighting the need to adopt GMP and HACCP procedures along every step of the milk and cheese production chain in order to reduce the public health risk linked to the consumption of foods contaminated by B. cereus s.l.