Applied Life Sciences Research Articles

High Fluorescence of Phytochromes Does Not Require Chromophore Protonation

Fluorescing proteins emitting in the near-infrared region are of high importance in various fields of biomedicine and applied life sciences. Promising candidates are phytochromes that can be engineered to a small size and genetically attached to a target system for in vivo monitoring. Here, we have investigated two of these minimal single-domain phytochromes, miRFP670nano3 and miRFP718nano, aiming at a better understanding of the structural parameters that control the fluorescence properties of the covalently bound biliverdin (BV) chromophore. On the basis of resonance Raman and time-resolved fluorescence spectroscopy, it is shown that in both proteins, BV is deprotonated at one of the inner pyrrole rings (B or C). This protonation pattern, which is unusual for tetrapyrroles in proteins, implies an equilibrium between a B- and C-protonated tautomer. The dynamics of the equilibrium are slow compared to the fluorescence lifetime in miRFP670nano3 but much faster in miRFP718nano, both in the ground and excited states. The different rates of proton exchange are most likely due to the different structural dynamics of the more rigid and more flexible chromophore in miRFP670nano3 and miRFP718nano, respectively. We suggest that these structural properties account for the quite different fluorescent quantum yields of both proteins.

Different scope of two applied biological chemistry journals as revealed by network analysis: a bibliometric study

Purpose: The landscape of academic publishing is experiencing significant transformations, characterized by an increasing volume of research output and the growth of interdisciplinary studies. These developments pose complex challenges for editorial boards, necessitating advanced strategies for submission management and the maintenance of publication standards.Methods: Utilizing network analysis, this study examined 1,865 articles from <i>Applied Biological Chemistry</i> and 1,081 articles from <i>Journal of Applied Biological Chemistry</i>, revealing distinct thematic and methodological orientations within these journals.Results: <i>Applied Biological Chemistry</i> demonstrated a pronounced focus on extraction processes, while <i>Journal of Applied Biological Chemistry</i> focused more on fermentation techniques and enzymatic studies. This differentiation highlights the journals’ unique contributions to the field of applied life sciences and underscores the diversity within academic publishing.Conclusion: The findings of this study not only shed light on the subtle distinctions between <i>Applied Biological Chemistry</i> and <i>Journal of Applied Biological Chemistry</i> but also emphasize the critical role of articulating the journal scope in detail in helping authors find the most suitable publication venues for their interdisciplinary research. By showcasing the utility of bibliometrics and network analysis, this research provides valuable insights for editorial boards to refine their management processes and for authors to navigate the complex landscape of academic publishing effectively, thereby enhancing the dissemination and impact of scholarly work.

Professor Manuel Miró, a researcher with an extensive and prestigious academic career, kindly spoke to BrJAC

Manuel Miró received his M.Sc. (1998) and Ph.D. (2002) in Chemistry from the University of the Balearic Islands, Spain. He has conducted post-doctoral research in several universities, including the Technical University of Berlin, Technical University of Denmark and University of Natural Resources and Applied Life Sciences. He is currently Full Professor in Analytical Chemistry at the University of the Balearic Islands (since September 2017); Guest Professor at Charles University (Czech Republic) (since 2014); and member of the IUPAC Chemistry and Environment Division (Subcommittee on Chemical and Biophysical Processes in the Environment). He has completed and consolidated four teaching periods (each of 5 years) and three research periods (each of 6 years). Dr. Miró is the Reviews Editor of the journal Analytica Chimica Acta (Elsevier, IF: 6.91, the second highest IF across scientific journals for general analytical chemistry) and Associate Editor of the Encyclopedia of Analytical Science, 3rd Edition, Elsevier, positions that he has held since 2007 and 2016, respectively. His publication record shows over 220 refereed publications, including 14 book chapters and a foreword, with an h-index of 42 and over 5600 citations. He has published 24 articles in the prestigious journal ‘Analytical Chemistry’ from the American Chemical Society and is the corresponding author of 110 articles. Dr. Miró has delivered 70 oral presentations (60 as plenary, keynote, or invited lecturer) at international conferences on analytical chemistry, sample preparation, nanotechnology, environmental chemistry, and automation based on flow methodology. He has also presented over 180 poster communications in international conferences and symposiums. He has supervised 10 Ph.D. students in national and international universities (Technical University of Denmark, Mahidol University and Chiang Mai University in Thailand, University of the Balearic Islands in Spain, and Federal University of Bahia in Brazil). He has been actively engaged in 34 national and international research projects (e.g., University of Melbourne in Australia and Charles University in Czech Republic), including 16 as the Principal Investigator. Dr. Miró’s research interests are focused on the development of on-line sample processing strategies for isolation and/or preconcentration of trace levels of environmental pollutants, exploiting 3D printing in the generation of various flow injections, including 3D-printed µFIA and Lab-on-a-Valve mesofluidic platforms, in conjunction with modern analytical instrumentation.

Nobuo Ikekawa

Nobuo Ikekawa, 95, died May 12 in Ito-shi, Japan. “Nobuo Ikekawa devoted his life to the study of chemistry. In doing so, he helped humankind by providing new medicines, including the active form of vitamin D, and by increasing crop production. He was awarded the Order of the Rising Sun, Gold Rays with Neck Ribbon, in 2002. He was a good educator with a generous heart. Every year he held a party at home for his students and family. He had treasured friends all over the world that he met through his career. In his private life, he enjoyed playing golf, raised three children, and had six grandchildren.”—Sumio Ikekawa, son Most recent title: President, Niigata University of Pharmacy and Applied Life Sciences Education: BS, pharmacy, 1951, and PhD, pharmacy, 1959, University of Tokyo Survivors: Wife, Sanae; sons, Sumio and Masao; daughter, Eiko Kasuga To recognize your late loved one or

Better understanding the exposome in order to maintain human health

Cumulative exposure to environmental factors and chemicals can be damaging to human health. A new concept called the exposome refers to the totality of exposures and their impact on health. Professor Yukiko Minamiyama, Food Hygiene and Environmental Health Division of Applied Life Science, Graduate School of Life and Environmental Sciences, Kyoto Prefectural University, is exploring how lifestyle factors could reduce exposure and maintain human health and also how certain lifestyle changes could control or avoid chronic oxidative stress. She and her team are delving deeper into the concept of the exposome and chronic oxidative stress to enhance understanding and facilitate informed, beneficial changes for humankind. Minamiyama is collaborating with a leading researcher on oxidative stress Dr Toshikazu Yoshikawa, Dr Hiroshi Ichikawa of Doshisha University School of Life and Medical Sciences and Dr Shigekazu Takemura of Osaka Metropolitan University Graduate School of Medicine. Minamiyama and the team believe controlling free radicals and reactive oxygen species (ROS) can prevent or improve diseases. Although food additives are unavoidable, Minamiyama believes that consuming antioxidants could help reduce oxidative stress and disease. In one study , the researchers investigated the in vitro and in vivo effects of food additives on the male rat brain and sperm/testes, with an emphasis on oxidative stress and found that the food additive stevioside induced ROS production in sperm and resulted in sperm dysfunction. They also discovered that treatment with the antioxidant alpha-tocopherol significantly improved oxidative stress.

Shape asymmetry - what's new?

Studies of shape asymmetry have become increasingly abundant as the methods of geometric morphometrics have gained widespread use. Most of these studies have focussed on fluctuating asymmetry and have largely obtained similar results as more traditional analyses of asymmetry in distance measurements, but several notable differences have also emerged. A key difference is that shape analyses provide information on the patterns, not just the amount of variation, and therefore tend to be more sensitive. Such analyses have shown that apparently symmetric structures in animals consistently show directional asymmetry for shape, but not for size. Furthermore, the long-standing prediction that phenotypic plasticity in response to environmental heterogeneity can contribute to fluctuating asymmetry has been confirmed for the first time for the shape of flower parts (but not for size). Finally, shape analyses in structures with complex symmetry, such as many flowers, can distinguish multiple types of directional asymmetry, generated by distinct direction-giving factors, which combine to the single component observable in bilaterally symmetric structures. While analyses of shape asymmetry are broadly compatible with traditional analyses of asymmetry, they incorporate more detailed morphological information, particularly for structures with complex symmetry, and therefore can reveal subtle biological effects that would otherwise not be apparent. This makes them a promising tool for a wide range of studies in the basic and applied life sciences.

Member's Plaza: Laboratory of Functional Plant Physiology Department of Applied Life Sciences Faculty of Agriculture, Shizuoka University/Plant Nutrition Laboratory, Department of Bioscience and Biotechnology, Kyushu University

Member's Plaza: Laboratory of Functional Plant Physiology Department of Applied Life Sciences Faculty of Agriculture, Shizuoka University/Plant Nutrition Laboratory, Department of Bioscience and Biotechnology, Kyushu University

Trypanocidal function of Terminalia catappa leaf extract in Albino rat

Trypanocidal function of Terminalia catappa leaf extract in Albino rat

"Palatability Evaluation, DNA Analyses, and Functional Processing of Rice"

Ken’ichi Ohtsubo* and Sumiko Nakamura Author Affiliations Faculty of Applied Life Sciences, Niigata University of Pharmacy and Applied Life Sciences, 265-1, Higashijima, Akiha-ku, Niigata, 956-8603, Japan Received: September 15, 2021 | Published: October 08, 2021 Corresponding author: David R Khan, Professor of Chemistry, Paul Engler Endowed Professor of Natural Sciences West Texas A&M University, USA DOI: 10.26717/BJSTR.2021.39.006282

Concept and Case Study for a Generic Simulation as a Digital Shadow to Be Used for Production Optimisation

Optimising an existing production plant is a challenging task for companies. Necessary physical test runs disturb running production processes. Simulation models are one opportunity to limit these physical test runs. This is particularly important since today’s fast and intelligent networking opportunities in production systems are in line with the call of Industry 4.0 for substantial and frequent changes. Creating simulation models for those systems requires high effort and in-depth knowledge of production processes. In the current literature, digital twins promise several advantages for production optimisation and can be used to simulate production systems, which reduce necessary physical test runs and related costs. While most companies are not able to create digital twins yet, companies using enterprise resource planning (ERP) systems have the general capability to create digital shadows. This paper presents a concept and a case study for a generic simulation of production systems in AnyLogic™ to create digital shadows as the first step towards a full digital twin. The generic simulation visualises production systems automatically and displays key performance indicators (KPIs) for the planned production program, using representational state transfer (REST) interfaces to extract product and production data from an ERP system. The case study has been applied in a learning factory of the University of Applied Life Sciences Emden/Leer. The results prove the presented concept of the generic simulation and show the limits and challenges of working with generic simulation models.

Preface

3rd INTERNATIONAL CONFERENCE ON FOOD SCIENCE AND ENGINEERING (ICFSE) 2020 International Conference on Food Science and Engineering (ICFSE) is a bi-annual conference series organized by the Faculty of Agriculture, Universitas Sebelas Maret (UNS), Surakarta, Indonesia since 2016. Following the success of previous ICFSE and great enthusiasms from the audience, the 3rd ICFSE is held on 29-30 September 2020 at UNS Surakarta. Unfortunately, the Covid-19 pandemic had spread worldwide during the year, causing a restriction to held a regular conference system. On the contrary, the 3rd ICFSE should not be postponed since participants have arranged to disclose their research finding in this conference as well as the uncertainty of when this pandemic will end. Therefore, in order to comply with WHO as well as the Indonesian Government regulation to cut the spread of Covid-19 by means of physical distancing, the committees of 3rd ICFSE 2020 have switched the conference format from a regular meeting into virtual meeting.This conference offers the theme “Strengthening Collaborative Frameworks for Innovations in Food Science and Engineering: Contributions to Sustainable Development Goals”. The transformational vision of the 2030 Agenda for Sustainable Development calls on all countries and stakeholders to work together to end hunger and prevent all forms of malnutrition by 2030. This goal can be achieved by ensuring the sustainability of agriculture and food systems, so that food supplies are stable and all people have access to adequate nutrition and health. Moreover, it must be realized that the sustainably supply of energy, clean freshwater, and foods for human kinds are among the major challenges that must be addressed for the future. An effective strategy must be conceptualized and implemented to feed the world through innovative research in the field of food science and engineering. This effort must be reinforced by the understanding and cooperation among the related parties all over the world in facing and solving the problems that might appears. Hence, the 3rd ICFSE 2020 is designed to facilitate the needs of academicians, researchers, practitioners, policy makers, governmental and private agencies in disclosing and sharing their knowledge and experiences in topic of current and future issues related to food science and engineering.The 3rd ICFSE 2020 featured a keynote speaker, Prof. Dr. Umar Santoso as the chairman of Indonesian Association of Food Technologist (IAFT) who delivered his speech for 40 minutes. Six prominent scientist and researchers in the field of food science and engineering worldwide were invited in this conference to share their expertise and experience in two plenary sessions. In each session, three invited speakers presented each 30 minutes talk followed by 45 minutes question and answer (Q&A) sessions for all speakers. The invited speakers including Prof. Dr. Ahmad Yunus (Department of Agrotechnology, Universitas Sebelas Maret, Indonesia), Prof. Dr. Mieke Uyttendaele (Department of Food Technology, Safety and Health, Ghent University, Belgium), Assoc. Prof. Dr. Jirawat Yongsawatdigul (School of Food Technology, Suranaree University of Technology, Thailand), Dr. Sangeeta Prakash (School of Agriculture and Food Science, University of Queensland, Australia), Prof. Dr. Hitoshi Shirakawa (Department of Science of Food Function and Health, Tohoku University, Japan), and Prof. Dr. Takahisa Nishizu (Department of Applied Life Sciences, Gifu University, Japan).As many of 123 papers from participants of six different countries had been accepted to be presented in the 3rd ICFSE 2020. They are clustered into 20 parallel sessions covering the following topics: food microbiology and biotechnology (3 sessions), food production system and technology (4 sessions), physical food analysis and chemistry (10 sessions) and miscellaneous topic (3 sessions). Time allocation of 10 minutes presentation and 5 minutes Q&A session was given to each presenter in a parallel session consisted of 5-7 presenter, 1 moderator and 1 time keeper. Both presentation and Q&A session were conducted live during virtual conference. The 3rd ICFSE 2020 virtual conference was technically run very smoothly via zoom online system by Universitas Sebelas Maret with no major issue arise during this conference. Similarly, there are no major issue regarding the virtual system on participant’s side that come from different part of Indonesia and oversea. Most of participants were very engaged throughout plenary and parallel sessions evidenced by many questions raised during Q&A sessions. Their comments on the implementation of this conference were very positive and satisfying.Lastly, we like to express our sincere gratitude to all of the 3rd ICFSE speakers, participants, sponsors, organizing committees and all of related parties who made this conference feasible. Their kind contributions are very much appreciated. Through this conference, we’ve shared our expertise and contributed our best effort to fight hunger and provide adequate nutritious food for all human kind.Best regards,Muhammad Zukhrufuz Zaman, Ph.D.Chairman of the 3rd ICFSE 2020Universitas Sebelas Maret (UNS), IndonesiaLIST OF COMMITTEES, CONFERENCE PHOTOGRAPHS, and the titles available in this pdf

Variation in Results of Three Biology‐Focused Search Engines: A Case Study Using North American Tree Species

Variation in Results of Three Biology‐Focused Search Engines: A Case Study Using North American Tree Species

Challenging Precedent: Critical Care Nursing and Medical Product Innovation.

In this presentation, I will share my unconventional journey, starting from my first job as a critical care staff nurse to my current role as tenure-track faculty at the University of Massachusetts Amherst, where I hold a joint position with the Institute for Applied Life Sciences and the College of Nursing. Throughout this journey, I have had many opportunities to participate in interdisciplinary clinical outcomes research and medical product development as a staff nurse, clinical nurse specialist, and project lead from the clinical, industry, and academic perspectives. While passionate about my central clinical research interests in technology innovation and its responsible use in critical and acute care, the foundation of my approach is dedicated to the values and lessons of my earliest experiences in critical care bedside nursing: supporting and preserving the dignity and humanity of person-centered patient care. Early in my career as a critical care nurse, I realized how vitally important a critical care nursing perspective could be in the design of technology for meeting the critical care needs of patients, nurses, and other professionals who provide this care. As the nation's largest group of health care professionals, nurses use more products than any other health care professional, and thus nurses have a uniquely practical and care-sensitive perspective on the development and design of medical products. Nurses, especially critical care nurses, are in a unique position to identify and address everyday health care issues, challenge assumptions and the status quo, address unrecognized and unarticulated needs, and ensure that clinical outcomes research serves as the foundation for validating the effectiveness of medical product innovation. My goal is to share lessons learned and to help participants to see the many different ways that critical care nursing knowledge can be used to improve patient care.

National Institutes of Health StrokeNet During the Time of COVID-19 and Beyond.

National Institutes of Health StrokeNet During the Time of COVID-19 and Beyond.

Challenging Precedent: Critical Care Nursing and Medical Product Innovation

In this presentation, I will share my unconventional journey, starting from my first job as a critical care staff nurse to my current role as tenure-track faculty at the University of Massachusetts Amherst, where I hold a joint position with the Institute for Applied Life Sciences and the College of Nursing. Throughout this journey, I have had many opportunities to participate in interdisciplinary clinical outcomes research and medical product development as a staff nurse, clinical nurse specialist, and project lead from the clinical, industry, and academic perspectives. While passionate about my central clinical research interests in technology innovation and its responsible use in critical and acute care, the foundation of my approach is dedicated to the values and lessons of my earliest experiences in critical care bedside nursing: supporting and preserving the dignity and humanity of person-centered patient care. Early in my career as a critical care nurse, I realized how vitally important a critical care nursing perspective could be in the design of technology for meeting the critical care needs of patients, nurses, and other professionals who provide this care. As the nation’s largest group of health care professionals, nurses use more products than any other health care professional, and thus nurses have a uniquely practical and care-sensitive perspective on the development and design of medical products. Nurses, especially critical care nurses, are in a unique position to identify and address everyday health care issues, challenge assumptions and the status quo, address unrecognized and unarticulated needs, and ensure that clinical outcomes research serves as the foundation for validating the effectiveness of medical product innovation. My goal is to share lessons learned and to help participants to see the many different ways that critical care nursing knowledge can be used to improve patient care.

The Novation Dynamic:: Clarifying the Work of Change, Disruption, and Innovation

Leading in today’s health care organizations is extremely challenging. The speed of technology and innovation, increasingly more regulatory requirements, and the demand for value-based care, all contribute to these challenges. Many of the current approaches used to develop the leaders of today and the future fall short of what is required for success. Innovation leadership must provide the skills and competencies to facilitate complex adaptive systems, complexity, change, and quantum leadership. In this article, “The Novation Dynamic: Clarifying the Work of Change, Disruption, and Innovation,” a framework for facilitating innovation is described. As the largest health care discipline and the only frontline health care providers with access to patients 24 hours/day, the nursing profession holds a unique perspective for understanding today’s health care challenges. Nurses are well-positioned to be the leaders of the Novation Dynamic, to provide the leadership required in today’s health care systems, and to work collaboratively with other health care disciplines, business colleagues, innovators, and patients. Leading in today’s health care organizations is extremely challenging. The speed of technology and innovation, increasingly more regulatory requirements, and the demand for value-based care, all contribute to these challenges. Many of the current approaches used to develop the leaders of today and the future fall short of what is required for success. Innovation leadership must provide the skills and competencies to facilitate complex adaptive systems, complexity, change, and quantum leadership. In this article, “The Novation Dynamic: Clarifying the Work of Change, Disruption, and Innovation,” a framework for facilitating innovation is described. As the largest health care discipline and the only frontline health care providers with access to patients 24 hours/day, the nursing profession holds a unique perspective for understanding today’s health care challenges. Nurses are well-positioned to be the leaders of the Novation Dynamic, to provide the leadership required in today’s health care systems, and to work collaboratively with other health care disciplines, business colleagues, innovators, and patients. Michael H. Ackerman, DNS, RN, FCCM, FNAP, FAANP, FAAP is director of the Master in Healthcare Innovation Program and professor of clinical nursing at the College of Nursing, The Ohio State University, in Columbus, Ohio. He can be reached at [email protected] . Karen K. Giuliano, PhD, RN, FAAN, MBA, is associate professor at the Institute for Applied Life Sciences & College of Nursing, University of Massachusetts Amherst, in Amherst. Kathy Malloch, PhD, MBA, RN, FAAN, is professor of clinical nursing at the College of Nursing, The Ohio State University, and associate director, Education & Evidence Based Regulation, Arizona State Board of Nursing in Phoenix, Arizona.

Material Development Using the Inherent Features of Nano-cellulose and Nano-chitin: Necessity of Simple Processes and Cross-disciplinary Collaboration

Abstract For nano-cellulose materials, which have attracted attention in both academia and industry in recent years, to be used as a truly valuable material class, the author believes that their processing procedures must be both rational and simple. In addition, it is important to focus on areas where the outcomes cannot be achieved without these particular nanomaterials. These points also apply to nanomaterials obtained from the animal structural polysaccharide chitin. On this basis, the author’s group has recently achieved “creation of micro-patterning cell culture substrate by inkjet printing of chitinous nanocrystals” and “utilization of cellulose nanofibers as a module for microfluidic paper-based analytical device (μPAD)”. The direction of these projects also reflects some of the activities of the applied life science department that the author worked for until recently. Cellulosic materials, including traditional forms for industrial use, often exist as a powder state at some point in the production process. The author hopes that research on nano-cellulose and nano-chitin will be enhanced through collaboration with powder technology researchers.

Material development using the inherent features of nano-cellulose and nano-chitin: Necessity of simple processes and cross-disciplinary collaboration

For nano-cellulose materials, which have attracted attention in both academia and industry in recent years, to be used as a truly valuable material class, the author believes that their processing procedures must be both rational and simple. In addition, it is important to focus on areas where the outcomes cannot be achieved without these particular nanomaterials. These points also apply to nanomaterials obtained from the animal structural polysaccharide chitin. On this basis, the author’s group has recently achieved “creation of micro-patterning cell culture substrate by inkjet printing of chitinous nanocrystals” and “utilization of cellulose nanofibers as a module for microfluidic paper-based analytical device (μPAD)”. The direction of these projects also reflects some of the activities of the applied life science department that the author worked for until recently. Cellulosic materials, including traditional forms for industrial use, often exist as a powder state at some point in the production process. The author hopes that research on nano-cellulose and nano-chitin will be enhanced through collaboration with powder technology researchers.

Magnetic Separation in Bioprocessing Beyond the Analytical Scale: From Biotechnology to the Food Industry.

Downstream processing needs more innovative ideas to advance and overcome current bioprocessing challenges. Chromatography is by far the most prevalent technique used by a conservative industrial sector. Chromatography has many advantages but also often represents the most expensive step in a pharmaceutical production process. Therefore, alternative methods as well as further processing strategies are urgently needed. One promising candidate for new developments on a large scale is magnetic separation, which enables the fast and direct capture of target molecules in fermentation broths. There has been a small revolution in this area in the last 10–20 years and a few papers dealing with the use of magnetic separation in bioprocessing examples beyond the analytical scale have been published. Since each target material is purified with a different magnetic separation approach, the comparison of processes is not trivial but would help to understand and improve magnetic separation and thus making it attractive for the technical scale. To address this issue, we report on the latest achievements in magnetic separation technology and offer an overview of the progress of the capture and separation of biomolecules derived from biotechnology and food technology. Magnetic separation has great potential for high-throughput downstream processing in applied life sciences. At the same time, two major challenges need to be overcome: (1) the development of a platform for suitable and flexible separation devices and (2) additional investigations of advantageous processing conditions, especially during recovery. Concentration and purification factors need to be improved to pave the way for the broader use of magnetic applications. The innovative combination of magnetic gradients and multipurpose separations will set new magnetic-based trends for large scale downstream processing.

Analysis of Lipid Droplet Content in Fission and Budding Yeasts using Automated Image Processing.

Lipid metabolism and its regulation are of interest to both basic and applied life sciences and biotechnology. In this regard, various yeast species are used as models in lipid metabolic research or for industrial lipid production. Lipid droplets are highly dynamic storage bodies and their cellular content represents a convenient readout of the lipid metabolic state. Fluorescence microscopy is a method of choice for quantitative analysis of cellular lipid droplets, as it relies on widely available equipment and allows analysis of individual lipid droplets. Furthermore, microscopic image analysis can be automated, greatly increasing overall analysis throughput. Here, we describe an experimental and analytical workflow for automated detection and quantitative description of individual lipid droplets in three different model yeast species: the fission yeasts Schizosaccharomyces pombe and Schizosaccharomyces japonicus, and the budding yeast Saccharomyces cerevisiae. Lipid droplets are visualized with BODIPY 493/503, and cell-impermeable fluorescent dextran is added to the culture media to help identify cell boundaries. Cells are subjected to 3D epifluorescence microscopy in green and blue channels and the resulting z-stack images are processed automatically by a MATLAB pipeline. The procedure outputs rich quantitative data on cellular lipid droplet content and individual lipid droplet characteristics in a tabular format suitable for downstream analyses in major spreadsheet or statistical packages. We provide example analyses of lipid droplet content under various conditions that affect cellular lipid metabolism.