The emerging area of genetically engineered ultrasound contrast agents, like gas vesicles, has the potential to broaden the applications of medical ultrasound imaging by enabling targeted and deep tissue imaging at the cellular level. Nevertheless, the existing gene construct/encoding relies on significant cell processing to ensure sufficient gas vesicles are formed within the cell to produce ultrasound contrast. Here, we describe a drug-inducible and drug-selectable acoustic reporter gene construct that can enable gas vesicle expression in mammalian cell lines, which we demonstrate in wild type HEK293T cells. Fluorescence microscopy was employed to validate the integration of the plasmid, while the creation of single-cell clones was achieved through the utilization of flow cytometry. The expression for gas vesicle was optically and ultrasonically verified, achieving 80% improved signal to noise ratio in cells expressing gas vesicles compared to negative controls. This technology introduces a novel paradigm for reporter genes, utilizing ultrasound to visually identify particular cell types both in vitro and in vivo, serving diverse purposes such as cellular reporting and applications in cell therapies.