Application Of Ultrasonic Waves Research Articles

Computational analysis of the simultaneous application of ultrasound and electric fields in a lipid bilayer

The combined application of electric fields and ultrasonic waves has shown promise in controlling cell membrane permeability, potentially resulting in synergistic effects that can be explored in the biotechnology industry. However, further clarification on how these processes interact is still needed. The objective of the present study was to investigate the atomic-scale effects of these processes on a DPPC lipid bilayer using molecular dynamics simulations. For higher electric fields, capable of independently forming pores, the application of an ultrasonic wave in the absence of cavitation yielded no additional effects on pore formation. However, for lower electric fields, the reduction in bilayer thickness induced by the shock wave catalyzed the electroporation process, effectively shortening the mean path that water molecules must traverse to form pores. When cavitation was considered, synergistic effects were evident only if the wave alone was able to generate pores through the formation of a water nanojet. In these cases, sonoporation acted as a mean to focus the electroporation effects on the initial pore formed by the nanojet. This study contributes to a better understanding of the synergy between electric fields and ultrasonic waves and to an optimal selection of processing parameters in practical applications of these processes.

A cavitation enabled green leaching of metals from spent lithium-ion batteries

As the world dabbles with increasing quantities of spent lithium-ion batteries (LIBs) and the need to recover valuable metals therein, sustainable approaches to achieving this need have become a necessity. In this work, we present the findings of an ultrasound-assisted intensification of a leaching process for the recovery of cobalt, nickel, manganese and lithium from spent LIBs. Molasses, methanesulfonic acid and ultrasonication were used together to enhance the leaching of the metals. The influence of ultrasonic modes, namely, sonication under isothermal and non-isothermal conditions, and leaching parameters, such as temperature, sonication amplitude, time, solid-liquid ratio, and reductant dosage, were evaluated. The leaching of metals was found to be strongly dependent on amplitude, temperature, and time. Compared to conventional mechanical stirring, the application of ultrasonic waves improves the leaching of metals by ∼22–74 %, with the degree of enhancement dependent on the temperature and the metal. The non-isothermal sonication mode (sonication without temperature control) has a lower energy input yet can yield a similar leaching performance as sonication under controlled temperature (isothermal mode). The results suggest that the introduction of ultrasonic waves created cavitation bubbles, which caused solid fragmentation, enhanced the mass transfer and diffusion of the reactants, and formed products that led to significant improvements in the leaching efficiency.

Application of therapeutic ultrasonic waves across the dialyzer membrane: A pilot study on the impact on dialyzer clearance and safety.

Progressive clogging of the dialyzer membrane during hemodialysis can compromise solute removal efficiency. Existing solutions fall short in addressing intradialytic reduction of dialyzer clearance. This pilot study aims to assess the impact and safety of applying therapeutic ultrasonic waves to dialyzers for mitigating intradialytic clogging. In this pilot study, 15 stable maintenance hemodialysis patients (12 males and 3 females) were enrolled. Each patient served as their own control. They underwent one session of hemodialysis with the application of therapeutic ultrasonic waves (Ultrasonic session) and were crossed-over to a second session without the use of ultrasonic waves (Control session). All the study sessions operated at a fixed dialysate flow rate of 500 mL/min and a blood flow rate of 250 or 300 mL/min. The adequacy of dialysis achieved during each session was monitored using Online Clearance Monitoring of the dialysis machines, and clearance K values, varying between 135 and 209 mL/min, were recorded, and plotted. A direct comparison between Control and Ultrasonic sessions was performed to assess the impact and safety of using ultrasonic waves during hemodialysis. The mean percentage decline in dialyzer clearance values was 4.41% for Ultrasonic sessions (SD: 5.3) and 12.69% for Control sessions (SD: 6.35) (p-value <0.001). This indicates that the application of ultrasonic waves reduced the decline in clearance values. The mean differences of the blood component parameters were comparable between both Ultrasonic sessions and Control sessions, suggesting the safety of utilizing ultrasonic waves during dialysis. Microscopic membrane analysis corroborated the safety. Intradialytic clogging of dialyzer membranes is a significant problem that can cause dialysis inadequacy. Our study tackles this issue by introducing therapeutic ultrasonic waves to improve dialyzer clearance during hemodialysis sessions in patients.

Effects of ultrasonic power on the electrochemical characteristics of high-performance nanosized zinc–cobalt oxide electrodes for asymmetric supercapacitors

This study reports the preparation of ZnCo2O4 oxide (ZCO) electrode materials with favorable microstructure and porosity arrangement via ultrasonic electrodeposition-calcination. The application of ultrasonic waves at a power of 200 W (using ZCO-2) led to the development of a smooth and flat microstructure, characterized by the presence of numerous micropores. The lattice constant of ZnCo2O4 in the ZCO-2 oxide electrode material was determined to be 0.428 nm via TEM analysis. Furthermore, the XRD analysis of ZCOs demonstrated the effective synthesis of ZnCo2O4 electrode materials. The main lattice diffraction planes of ZCOs were observed at (111), (220), (311), (400), (511), and (440), with corresponding diffraction angles of 29.8°, 36.4°, 42.9°, 45.2°, 62.3°, and 66.5°, respectively. The application of 200 W ultrasonic power during electrochemical experiments significantly improved the electrochemical performance of ZCO-2. The ZCO-2 oxide electrode demonstrated remarkable area capacitance and negligible electrical impedance. At a current density of 3 mA/cm2, the area capacitance was measured to be 1.803 F/cm2. The ZCO-2 oxide electrode showed remarkable capacitance retention of 67.3 % even under high current density conditions. Furthermore, the asymmetric supercapacitor composed of ZCO-2 and AC (ZCO-2/AC ASC) showed outstanding electrochemical performance. The ZCO-2/AC ASC showed a capacitance of 5.721 F/cm3 at a current density of 0.5 mA/cm2. Furthermore, it demonstrated an energy density of 68.2 Wh/kg at a power density of 250 W/kg. The capacitor showed consistent performance and retained 63.8 % of its initial capacitance even after undergoing 24,000 cycles. Furthermore, the findings of this research provide substantial implications for the combination of ultrasonic electrodeposition with capacitors, while also presenting an innovative concept for electrode materials in high-performance supercapacitors.

Application of ultrasonic waves to evaluate the rheological properties of non-Newtonian slurries: a preliminary study

An ultrasonic-wave system was developed to measure the rheological properties of non-Newtonian materials based on their responses to shear and pressure waves. This system was composed of a commercial shear wave transducer and various homemade devices, including a signal generator, a signal conditioner, and a transmission/receiving switch. The shear wave reflections at the interface between the delay line and the unknown test materials can be interpreted by the signals measured with calibration materials (e.g. air, water, olive oil and honey) that have well-known rheological properties. If the Voigt viscoelastic model is used to describe the dynamic behaviour of these test materials, the interpreted data can be used to determine the acoustic impedances, and thus solve for their shear moduli (G), kinematic viscosities (ν) and bulk densities (ρ). After the calibration procedures, the proposed system was used to test a consolidating kaolinite slurry with zero salinity and an initial suspended sediment concentration of 420 g l–1. The measurements showed that the shear modulus G is developed until consolidation for approximately 100 h. Afterward, the G gradually approached a value on the order of 500 N m–2 after approximately 200 h. This corresponded to an initial viscosity of approximately 7 × 10−5 m2 s–1, which decreased slowly over time to approximately 4 × 10−6 m2 s–1 after 300 h. With minor modification to capture pressure waves from the shear wave transducer, the bulk density can also be measured. The integrated system offers a non-invasive and efficient method for characterizing non-Newtonian fluids, providing valuable insights into their rheological properties, which are crucial for a wide range of industrial and scientific applications.

Investigation of renal calculi fragmented tracer particles in lithotripsy model by laser speckle technique

We have developed a laser sheet method to evaluate micro-sized fragmented calcium oxalate granular particles created kidney stones suspended in a Newtonian fluid in an in vitro model. These tracer particles are examined by laser optical techniques. An optical setup is configured with a laser sheet to conduct image velocimetry on these calcium oxalate seed particles in the urine environment. The experimental setup involves the application of ultrasonic waves to fragment the calcium oxalate tiny stones of varying sizes and disperse them in random directions in the fluid. The data acquisition process employs double frame-single exposure imaging, which captures images at specified time intervals using a high-resolution CCD camera. This provides information regarding particle displacement and track the flow path within the Newtonian fluid. In addition, the bigger fragmented particles are identified, and their sizes are also measured.

Effect of in situ ultrasonic wave and influent ammonia nitrogen fluctuation on stability of aerobic granular sludge

ABSTRACT This study elucidates the impact of fluctuating influent conditions and in situ ultrasonic wave exposure on the stability of aerobic granular sludge (AGS) in the treatment of simulated wastewater emanating from rare earth mining operations. During a stable influent period spanning from Day 1 to Day 95, the seed granules underwent an initial disintegration followed by a re-granulation phase. The secondary granulation was achieved on Day 80 and Day 40 for the ultrasonic reactor (R1) and the control reactor (R2), respectively. Notably, granules formed in R1 exhibited a more porous structure compared to those generated in R2. Subsequently, when the ammonia nitrogen in the influent oscillated between 100 and 500 mg/L during Days 96-140, both reactors yielded compact and densely structured granules. Nitrogen removal profiles were comparable between the two reactors: the removal efficiencies for ammonia nitrogen and total inorganic nitrogen escalated from 95% and 80%, respectively, during Days 1-95, to 95% and 90%, respectively, post-Day 140. A suite of performance metrics indicated that steady-state granules from R1 outperformed those from R2 across several parameters. Specifically, the nitrification/denitrification rates, and relative abundance of denitrifying bacteria were all higher in granules from R1. Conversely, the relative abundance of nitrifying bacteria was comparable between granules from both reactors. However, R1 granules demonstrated lower sludge concentration and smaller average particle size than their R2 counterparts. In conclusion, the AGS system demonstrated robust resilience to fluctuating ammonia nitrogen, and the application of ultrasonic waves significantly enhanced granular activity while achieving in situ sludge reduction.

Improving heavy metal removal efficiency from steel sludge: Application of the coupled ultrasonic-bioleaching treatment

&lt;p&gt;This study assessed the efficiency of Fe, Al, Ni and Sr removal from the steel sludge using the coupled bioleaching with Thiobacillus thiooxidans and ultrasonic waves. Growth conditions were optimized using the surface response method. The bacterium was adapted successively to three heavy metal-containing solutions with different concentrations of 100, 200, and 300 mg/ml. Samples were exposed to ultrasonic waves at frequencies of 30, 60 and 90 kHz and durations of 20, 30 and 40 min for two weeks. The highest Fe removal efficiency of 98.45% was obtained using the T. thiooxidation, wave frequency of 30 kHz for 40 min, and pulp density of 100 mg/ml. The maximum removal efficiency was found to be 99.74% for Al under a wave frequency of 90 kHz for 20 min and a pulp density of 300 mg/ml, approximately 100% for Ni under a wave frequency of 30 kHz for 20 min and a pulp density of 300 mg/ml, and 98.45% for Sr under a wave frequency of 90 kHz for 20 min and a pulp density of 300 mg/mL. Results showed that the removal efficiency of Ni and Al bioleaching improved significantly (P &amp;lt;0.05) under the ultrasonic irradiation while the removal efficiency of Fe and Sr remained statistically unchanged (P&amp;gt; 0.05) with and without the application of ultrasonic waves.&lt;/p&gt;&#x0D;

Unveiling the Potential of Cavitation Erosion-Induced Heavy Crude Oil Upgrading

The application of ultrasonic waves in the processing of hydrocarbons is a new promising technology that has developed rapidly in recent years. However, the acoustic-induced cavitation erosion phenomenon is poorly studied. In this paper, a comparison study of cavitation erosion was carried out in water and oils with different viscosities produced from Ashal’cha and North Komsomol using an ultrasonic reactor operating at an industrial frequency of 20 kHz. The acoustic spectra obtained from hydrophones during the ultrasonic treatment of fluids can be characterized by using subharmonics of the main frequency and a continuous white noise level. Moreover, the cavitation erosion of aluminum foil under various ultrasound irradiation times and power levels was thoroughly investigated. It has been found that the process of ultrasonic cavitation has a less erosive impact on metal foil in oil due to its high viscosity. In addition, the formation of microflows in the oil phase, which also intensify the erosion process, is hindered. Cavitation erosion in the Ashal’cha oil sample exhibited a higher intensity compared to that in the North Komsomol oil sample. It was found that upon increasing ultrasound intensity in the case of the viscous (Ashal’cha) oil sample, cavitation stability was disrupted. In turn, this led to a reduction in the collapse energy of the cavitation bubbles. The results we obtained enable the assessment of cavitation erosion in crude oil and could be used to improve methodologies for monitoring and optimizing cavitation processes in crude oil.

An Application of Ultrasonic Waves in the Pretreatment of Biological Sludge in Urban Sewage and Proposing an Artificial Neural Network Predictive Model of Concentration

This research examines whether ultrasonic waves can enhance the hydrolysis, stability, and dewatering of activated sludge from raw urban wastewater. Sampling and physical examination of the activated sludge that was returned to the aeration pond were carried out using ultrasonic waves that were guided at frequencies of 30 and 50 kHz for periods of 0.5, 1, 3, 5, 10, 15, and 30 min. Various tests, including volatile suspended solids, inorganic solids, volatile solids, sludge resistant time, capillary suction time, total suspended solids, total solids, and volatile soluble solids, were carried out to advance further the processes of hydrolysis, stabilization, and dehydration of samples. According to the observations, the volatile soluble solids at a frequency of 30 kHz and t=15 min were raised by 72%. The capillary suction time of 30 and 50 kHz in 1 min demonstrated a drop of 29 and 22%, respectively. It is crucial to consider that, at 10 min and the frequency of 50 kHz, the greatest efficiency was found. The 30 kHz and 1 min yielded the optimum sludge dewatering conditions. Finally, artificial neural networks (ANN) are utilized to propose predictive models for concentration, and the results were also very accurate (MAE=1.37%). Regarding the computational costs, the ANN took approximately 5% of the time spent on experiments.

Green Application of Ultrasonic Waves for Extraction of Yellow Colorant from Haar Singhar and its Colouring Behaviour in Cotton Dyeing

Natural dyes have grown in popularity due to their eco-friendliness. They can be used as a substitute for synthetic dyes to minimize environmental pollution. This research investigates the natural colouring behaviour of haar singhar flower (HSF) extract in cotton dyeing. Colorant was extracted under various conditions utilizing various extraction mediums such as aqueous, basic, and acidic. On cotton, it was discovered that applying 55 mL of aqueous extract containing 1.5 g/100 mL sodium chloride for 40 minutes at 70 °C yielded the best colour output. A new hue with good colour fastness was developed utilizing chemical and bio-mordants. The existence of nyctanthin as a colouring agent in haar singhar flowers was discovered through FTIR analysis of the extract. The CIE Lab system revealed that using 2 g/100 mL of pistachio shell as a bio-mordant resulted in good quality reddish yellow hues. It was discovered that ultrasonic radiations have a high potential for isolating colourants and dyeing cotton fabric under decreased conditions of temperature, time and volume. The application of biomordants has made the procedure greener, more efficient, and safer.

Ultrasonic sensing of properties of eco-friendly cement mortar

Cement is one of the essential construction materials required to develop concrete and mortar; therefore, it is vital in developing cities' infrastructure and maintaining human civilisation. However, cement production is recognised as a major source of many environmental problems, including air and water pollution. This paper, therefore, focused on the partial replacement (from 0 to 40%) of cement in the mortars with industrial by-products and on the application of ultrasonic waves as a sensing approach to evaluate the mechanical properties of the new cement mortar. The compressive strength of the eco-friendly mortar was measured at ages of 7, 14, and 28 days, and the results showed the best compressive strength of the eco-friendly mortar was 19.8 MPa at 28 days compared to 23.9 MPa of the reference mix. Additionally, a good agreement was noticed between the ultrasonic pulse velocity (UPV) and the compressive strength confirming the applicability of the UPV for compressive strength sensing.

The Application of Ultrasonic Waves and Microwaves to Improve Antihyperglycaemic and Antimicrobial Activities of Marrubium vulgare Extracts.

Having scarce information about ultrasound assisted extraction (UAE) and microwave assisted extraction (MAE) of white horehound (Marrubium vulgare L.), the idea has emerged to determine the optimal process parameters for the maximization of polyphenols and to compare the efficiency of these green extraction technologies. The optimal UAE parameters are temperature of 73.6 °C, extraction time of 40 min and ultrasound power of 30.3 W/L, while the optimal MAE parameters are 63.8% ethanol, extraction time of 15 min and microwave power of 422 W. Extract obtained at optimal UAE parameters shows the highest antihyperglycemic activity (α-amylase inhibition: 50.63% and α-glucosidase inhibition: 48.67%), which can potentially be explained by the presence of chlorogenic acid and quercetin, which were not identified in the macerates. The most sensitive bacterial strain to optimal ultrasonic extract is Bacillus cereus, whereas the most sensitive fungal strain is Saccharomyces cerevisiae.

Predictive models in the ultrasonic disinfection of hospital-effluents: a review and suggestion for future research

At the end of this review on applications of ultrasonic waves in the inactivation of microorganisms in hospital laundry effluents, the fourth type of predictive model is proposed, based on the application characteristics of the experimental plans. The number of works for the disinfection of wastewater by ultrasound waves (US) associated with other techniques or individuals has been increasing. The use of ultrasound to produce lethal effects on microorganisms is attractive because it is considered a "green" technology in that it involves sound energy and does not require additional chemicals, or a very small amount. Also, the US as a tool for water reuse processes - operates at low pressure and temperature and does not produce toxic or greenhouse gases. Hospital laundry effluents are characterized by different microorganisms, a fact that can hinder the application of conventional physical-chemical technologies. To contribute to future developments in the application of ultrasound (US), this work uses some typical US applications in the disinfection of aqueous effluents. To propose strategies for carrying out new works, comments are made regarding an approach oriented towards new advances based on the need to interrelate the variables involved in the studies, at the same time that these variables are correlated with the rate of inactivation. For this, it is discussed the predictive models and their contributions, according to the number of factors that should be involved for a better understanding of the rate of microbial inactivation.

RANCANG BANGUN SOUNDINGMETER FUEL OIL KAPAL PENANGKAP IKAN BERBASIS MIKROCONTROLLER ATMEGA328

This study discusses the design of a sounding system that measures the liquid contents in a vessel tank. The fuel tank sounding system on the ship still uses manuals. In this case it can be a problem that is often faced because it has not used an automated system. Monitoring the measurement of the level of fuel in the tank using a measuring line. In this Final Project a fuel tank monitoring model has been designed on the ship using the application of ultrasonic waves by reflecting sound waves. This monitoring model is to make a system know if the fuel in the tank on the ship has started to run out. The device system consists of a transmitter, an ultrasonic receiver based on the ATMega326 microcontroller. Transmitter and receiver are directed to the tank. Where the distance is directly proportional to the transmitter's transmitting time until it is received back by the receiver, then processed using the ATMega326 microcontroller as the main controller, and the LCD as a display of the state of the fuel that is in the tank of the ship.

Recent Advancement of Phase Shifted Fiber Bragg Grating Sensor for Ultrasonic Wave Application: A Review

Fiber Bragg grating (FBG) is gaining traction in the field of optical fiber sensors, either as a detection tool for ultrasonic waves or as a detection target. The significant contributions of the last few years are summarized in this overview, that includes setups based on wavelength and intensity. This article summarizes recent research and development activities in ultrasonic wave applications utilizing FBG sensors and makes recommendations for future research. Several different FBG strain sensor packing configurations are discussed. This article presents a comprehensive theoretical and mathematical analysis of a phase-shifted fiber Bragg grating (<inline-formula> <tex-math notation="LaTeX">$\pi $ </tex-math></inline-formula>-PS-FBG) for use in a structural health monitoring system that employs a longitudinal ultrasonic strain wave obtained via a natural single FBG. The effect of grating regulating factors (grating length, index change, and apodization), as well as ultrasonic acoustic wavelengths, on the <inline-formula> <tex-math notation="LaTeX">$\pi $ </tex-math></inline-formula>-PS-FBG&#x2019;s wavelength shift sensitivity have been investigated. Additionally, the slow-light effect in the <inline-formula> <tex-math notation="LaTeX">$\pi $ </tex-math></inline-formula>-PS-FBG acoustic sensor was investigated. Sensitivity, detection range, and limit of detection are all thoroughly examined and discussed with the objective of comparing various technologies and determining future directions in the field of phase shifted fiber optic sensors.

Theoretical and experimental study of a new freezing desalination system assisted by ultrasound

Compared with traditional seawater desalination methods, freezing desalination has gotten more attention for its advantages in reducing energy consumption and environmental protection. In this study, a new freezing desalination assisted by ultrasonic is proposed to improve desalination efficiency. A detailed freezing desalination model is established based on phase transition analysis and ultrasonic-assisted freezing theory. The experiment system is also developed to analyze the desalination process. The factors such as temperature, interface transformation and salinity in the process of droplet freezing under the action of ultrasound were analyzed and discussed. The result showed that the salinity of the droplet residual brine could decline to less than 2.4% when the initial salinity was 5% and droplet diameter was 2 mm after 1 min centrifugal treatment. After the treatment, the salinity of the existed ice-brine mixing was decreased below 0.2%. According to experiments and simulation analysis, the application of ultrasonic wave in freezing desalination can effectively increase the freezing speed of droplets and improve the efficiency of desalination. This study will contribute to the application of ultrasonic in the freezing desalination.

Solving ultrasonic ray tracing in parts with multiple material layers through Root-Finding methods

Ultrasonic testing has been used for material analysis and inspection since 1930′s. Nevertheless, the applicability of ultrasonic waves to new complex cases is still growing, thanks to the availability of powerful electronics and software. However, the complication that slows down the deployment of ultrasonic inspection to geometric complex parts and structures arises from the wave refraction phenomenon. A clear understanding of the ultrasound wave propagation, impacted by refractions, is crucial to interpret the data obtained from the inspection of multi-layered/multi-medium test subjects as it is not always possible to assume that mechanical waves travel in straight lines. This work presents suitable approaches for solving the ray-tracing problem in multi-layered structures. Accurate benchmarking shows that the use of the Newton-Raphson root-finding method allows a threefold reduction of the computation time, when compared to the bisection-based root-finding methods. An effective combination of the Newton-Raphson methods with bisection-type iterations is also proposed and discussed. Although the work repeatedly refers to the field of ultrasonic inspection, the presented findings are relevant and applicable to areas beyond material inspection.

Influence of intensity of ultrasound on morphology and hardness of copper coatings obtained by electrodeposition

The influence of various intensities of ultrasound applied for the electrolyte stirring on morphological and mechanical characteristics of electrolytically produced copper coatings has been investigated. The copper coatings produced by the galvanostatic regime of the electrodeposition from the basic sulphate electrolyte and the electrolyte with added levelling/brightening additives at the low temperature were characterized by scanning electron microscopy (SEM) and atomic force microscopy (AFM) techniques (surface morphology and topography, respectively) and by Vickers microindentation (hardness). The roughness of coatings increased with the increasing intensity of ultrasound, indicating that morphology of the coatings worsened with the enhanced application of ultrasonic waves. This is attributed to the strong effect of ultrasound on hydrodynamic conditions in the near-electrode layer, which is manifested by the increase of share of the activation control in the mixed activation-diffusion control of electrodeposition with increasing the intensity of ultrasound. The concept of "effective overpotential" originally proposed to explain a change of surface morphology in the conditions of vigorous hydrogen evolution is also applicable for a change of morphology of Cu coatings under the imposed effect of ultrasonic waves. Hardness analysis of the coatings showed that an intensity of applied ultrasound did not have any significant effect on the hardness, especially for the Cu coatings produced from the basic sulphate electrolyte.

Study on the application of ultrasonic wave in the removal of microbiofouling on PMMA surface

Ultrasound has been used for antifouling on the surface of medical devices or food utensils, but it is rarely applied in marine anti-biofouling on underwater instruments. To understand whether ultrasonic antifouling is suitable for underwater optical windows, the effect of ultrasonic conditions including frequency, power and duration on the removal of microbiofouling on the surface of polymethyl methacrylate (PMMA), a type of common optical material, was investigated in this study by three-factor and three-level orthogonal experiments. Before and after the ultrasonic treatment, both surface morphology and fouling degree of PMMA samples immersed in Escherichia coli suspension and seawater were characterized and quantified using laser scanning microscope. The results showed that ultrasonic treatment can effectively remove microfouling from the PMMA surface under suitable conditions. Ultrasonic technology has a great potential for the control of microfouling on the marine optical instruments. When compared with power and duration, ultrasonic frequency has a more significant effect on antifouling efficacy of ultrasound. It is useful for PMMA samples exposed to seawater within 2 days to conduct an antifouling treatment under the condition of an ultrasonic frequency of 20 kHz, ultrasonic power of 40 W, and ultrasonic duration of 7 min.