Abstract
We have developed a laser sheet method to evaluate micro-sized fragmented calcium oxalate granular particles created kidney stones suspended in a Newtonian fluid in an in vitro model. These tracer particles are examined by laser optical techniques. An optical setup is configured with a laser sheet to conduct image velocimetry on these calcium oxalate seed particles in the urine environment. The experimental setup involves the application of ultrasonic waves to fragment the calcium oxalate tiny stones of varying sizes and disperse them in random directions in the fluid. The data acquisition process employs double frame-single exposure imaging, which captures images at specified time intervals using a high-resolution CCD camera. This provides information regarding particle displacement and track the flow path within the Newtonian fluid. In addition, the bigger fragmented particles are identified, and their sizes are also measured.
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