Veterinary Applications Research Articles

Species-Specific Optimisation of Cryopreservation Media for Goat and Buffalo Adipose-Derived Mesenchymal Stem Cells.

Adipose-derived mesenchymal stem cells (ADSCs) are promising for clinical and veterinary applications due to their ease of isolation, high yield, and multilineage differentiation potential. Effective cryopreservation is vital to ensure their availability for large-scale applications. This study evaluated cryopreservation strategies for goat (gADSCs) and buffalo (bADSCs) ADSCs, using combinations of intracellular (dimethyl sulfoxide, DMSO) and exocellular cryoprotectants, including fetal bovine serum (FBS), polyethylene glycol (PEG), trehalose, bovine serum albumin (BSA), and dextran. Post-thaw parameters such as viability, recovery, metabolic activity, clonogenicity, oxidative stress, apoptosis, and senescence were assessed. Results revealed species-specific differences in cryopreservation requirements. gADSCs were optimally preserved in a medium with 5% DMSO, 3% FBS, 2% PEG, 3% trehalose, and 2% BSA, while bADSCs performed best in an FBS-free medium containing 5% DMSO, 2% PEG, 3% trehalose, and 2% BSA. DMSO-FBS formulations supported high recovery and metabolic activity but were associated with increased oxidative stress and apoptosis. Dextran-based cryomedia effectively preserved gADSCs but failed to maintain bADSC functionality. Biochemical composition analysis indicated significantly higher lipid content in bADSCs, likely influencing cryopreservation efficacy. These findings underscore the need for tailored cryopreservation strategies to address species-specific differences. Incorporating exocellular cryoprotectants reduced FBS dependency, minimised oxidative damage, and maintained functional attributes. This study highlights the potential for optimised, cost-effective biobanking solutions that accommodate species-specific requirements, advancing the use of ADSCs in veterinary and translational research.

Comprehensive Echocardiographic Protocol for Pigs with Emphasis on Diastolic Function: Advantages over MRI Assessment.

Swine are increasingly utilized in cardiovascular research due to their anatomical and physiological similarities to humans, particularly for studying diastolic dysfunction. While MRI offers excellent structural imaging, echocardiography provides superior real-time assessment of diastolic parameters. To address the lack of standardized methods and reduce variability across studies, we present a comprehensive guide for performing echocardiography in Yorkshire pigs, detailing anatomical considerations, equipment requirements, and technical approaches. We describe systematic approaches for obtaining and optimizing right parasternal long and short-axis views, apical four-chamber, and subcostal imaging windows, with specific attention to anatomical variations from human cardiac orientation and standard clinical transducer positioning. These tomographic views enable comprehensive assessment of systolic and diastolic function, including ventricular volumes, wall thicknesses, chamber dimensions, ejection fraction, and Doppler measurements of blood flow and tissue velocities. This standardized methodology for echocardiographic images acquisition enhances data reliability in cardiovascular pig models, improving the interpretation of preclinical study results and strengthening translational research outcomes. The protocol also provides consistency for veterinary applications, making echocardiography a preferred modality for longitudinal studies in this valuable translational model.

Age estimation using the ratio of dental pulp to tooth volume by CT scan in dogs.

Accurate age estimations in dogs are essential for various veterinary and clinical applications. Traditional methods based on dental appearance are error-prone because of external influences, necessitating the development of more precise methodologies. This study examined the association between chronological age and pulp to tooth (P/T) volume ratio in dogs using computed tomography (CT) of the right maxillary canine and right maxillary fourth premolar (PM4) teeth. Ninety-five canine skulls, aged five to 216 months, were examined using a two-slice CT scan machine. Volumetric analyses of the right maxillary canine and PM4 were performed. Statistical analysis, including Spearman's correlation and regression analysis, was conducted to assess the relationship between age and the P/T volume ratio. This study included 60 male and 35 female dogs, with no significant age difference between genders (p = 0.655). A strong negative correlation was observed between age and the P/T volume ratio for the right maxillary canine (r = 0.88, p < 0.001) and PM4 (r = 0.77, p < 0.001) teeth. Logarithmic regression models provided a better fit for age prediction (R² = 0.78 for the right maxillary canine, R² = 0.67 for PM4) compared to linear models. CT-based volumetric analysis of the P/T ratio in the right maxillary canine and PM4 teeth offers a precise, non-invasive method for estimating the age of dogs. This technique can improve the accuracy of age estimations, particularly in forensic and clinical settings, providing valuable insights for veterinarians and researchers.

Stem Cell-Based Therapies for Glaucoma Treatment: A Review Bridging the Gap in Veterinary Patients.

Retinal diseases are characterized by progressive damage to retinal cells, leading to irreversible vision loss. Among these, glaucoma stands out as a multifactorial neurodegenerative disease involving elevated intraocular pressure, retinal ganglion cell apoptosis, and optic nerve damage, ultimately resulting in blindness in both humans and dogs. Stem cell-based therapies have emerged as a promising therapeutic option for such conditions due to their regenerative and neuroprotective potential. These therapies, particularly those based on mesenchymal stem cells, offer the potential to repair and protect retinal tissues through the bioactive molecules (growth factors, cytokines, chemokines) secreted, their secretome. However, research in this field, especially on the use of umbilical cord mesenchymal stem cells' secretome, remains sparse. Most clinical trials focus on human glaucomatous patients, leaving a significant gap in veterinary patients' application, especially in dogs, with additional research being needed to determine its usefulness in canine glaucoma treatment. Future studies should aim to evaluate these therapies across both human and veterinary contexts, broadening treatment possibilities for glaucoma.

DOG BREED CLASSIFICATION USING TRANSFER LEARNING

“Dog Breed Classification using Transfer Learning “ the project focus on accurately classifying dog breeds is a challenging computer vision task due to the subtle differences between breeds and the high variability within breeds. This project addresses these complexities using transfer learning with the Inception V3 model, a state-of-the-art convolutional neural network (CNN). Traditional machine learning approaches often struggle with subtle inter-breed differences and require large labeled datasets and significant computational resources. Training deep learning models from scratch is typically infeasible, which makes transfer learning an ideal solution. By using a pre-trained Inception V3 model, this approach leverages learned features from large-scale datasets like ImageNet and fine-tunes them for dog breed classification, reducing trainingtime while enhancing accuracy. The fine- tuned Inception V3 model undergoes transfer learningwhere initial layers remain frozen to retain general features, and fine-tuning optimizes the later layers for specific breed classification. To make the solution accessible and user-friendly, a Flask web application is developed. Users can upload dog images and receive breed predictions in real time, with the backend integrating the trained model to process the images, extract features, and output predictions. The application is scalable and practical for diverse deployment scenarios, such as pet identification, educational purposes, or veterinary applications. This project not only highlights the efficiency of transfer learning in reducing training time but also demonstrates how AI can be applied effectively in real-world challenges. Combining advanced deep learning with a simple web interface bridges the gap between complex machine learning models and practical applications, emphasizing accuracy, efficiency, and accessibility in pet care and animal research. Key Words: Inception v3, fine-tuning , CNN , flask

Single Cell Transcriptomic Profiling of MYBPC3-Associated Hypertrophic Cardiomyopathy Across Species Reveals Conservation of Biological Process But Not Gene Expression.

Hypertrophic cardiomyopathy (HCM) is a common heritable heart disease where the most frequently associated mutations occur in the myosin-binding protein C (MYBPC3) sarcomere-associated gene. HCM is also a common veterinary clinical problem in certain cat breeds such as Maine Coons and Ragdolls, also most associated with mutations in MYBPC3. Mouse models of HCM in which Mybpc3 mutations are introduced recapitulate some, but not all, features of human HCM. To elucidate the common and distinctive pathological pathways across species and foster a greater understanding of the concordance of mouse HCM models to clinical mybpc3-associated HCM, we generated single nuclei RNA-sequencing data sets from feline, human, and murine heart tissue carrying MYBPC3 variants. Numerous genes were differentially expressed between mutation positive and mutation negative cell types within each species, identified using the model-based analysis of single-cell transcriptomics algorithm. Gene Ontology enrichment analysis of differentially expressed genes in cardiomyocytes across species revealed alterations in genes involved in muscle development, muscle contraction, muscle hypertrophy, regulation of sarcoplasmic calcium release, ATP metabolic process, and oxidative phosphorylation. These common biological processes across species are consistent with known phenotypic aspects of HCM such as hypertrophy, hypercontractility, diastolic dysfunction, and altered energy metabolism. Surprisingly, among conserved biological processes within cardiomyocytes across species, the individual genes driving the biological processes were distinct. This work to identify common and species-specific disease-promoting pathway differences will allow development of targeted therapies for both human and veterinary application and will facilitate an understanding of the idiosyncrasies of mouse models.

Recombinant VSV as a platform for rabies vaccine development: Enhancing immunogenicity and safety

The research investigates the possibility of development of a recombinant vesicular stomatitis virus (VSV) expressing the rabies virus glycoprotein as a novel rabies vaccine. The recombinant VSV can show robust immunogenicity, eliciting strong virus-neutralizing antibody responses in both mice and dogs. The oral vaccine formulation proved stable in simulated gastric conditions and induced immune responses comparable to intramuscular injection. Long-term immunity studies indicated high antibody levels and protection against rabies virus challenge at six months and one-year post-immunization, suggesting potential for durable immunity, particularly in dogs. Toxicity studies can be used to analyze adverse effects, confirming the vaccine’s safety. The potential for the recombinant VSV vaccine to induce mucosal immunity and spread through social behaviours in wildlife populations presents a promising approach for rabies control. However, challenges such as ensuring genetic stability and evaluating environmental impacts must be addressed. This research highlights the recombinant VSV-based vaccine’s promise as an effective, safe, and potentially self-disseminating tool for rabies prevention, with implications for both human and veterinary applications.

A Nanotoxicological Approach to the Effects of Metformin and Sodium Metavanadate Co-encapsulated in Polycaprolactone Nanoparticles under the Biological Parameters of Zebrafish (Danio rerio)

The present study evaluated the sublethal and lethal effects, and heart rate of Danio rerio when exposed to antidiabetic drugs, metformin (M-10), and sodium metavanadate (V-10), their association (MV-10) and the polycaprolactone nanoparticles containing poloxamer 188 with (PPMV-1, PPMV-5, and PPMV-10) or without these antidiabetics (PP-10). Acute toxicity tests were carried out to evaluate these effects. Groups V-10, MV-10, PPMV-5, PPMV-10, and PP-10 had lethal and sublethal, such as effects as pericardial edema. Concerning the heart rate, the PPMV-1, PPMV-5, PPMV-10, and PP-10 groups had a reduction compared to the other groups, indicating toxicity of the constituents of the nanoparticles. PPMV-5, PPMV-10, and PP-10 groups had sublethal and lethal effects depending on the concentration. Antidiabetics were eliminated as a possible cause and the poloxamer 188 is non-toxic at the concentrations used. In the PP-10 group, there was a dynamic of sublethal and lethal effects like the PPMV-10 group. We conclude that the presence of PCL in the formulation of nanoparticles was harmful at the cardiovascular level in embryos and eleuthero-embryos of D. rerio, affecting their development and heart rate, regardless of their concentration. Because of the results obtained, we can conclude that the toxicological evaluation of a nanomaterial is important to anticipate problems in its veterinary application since such results show possible toxicity problems.

Predicting Admission and Future Performance of Veterinary School Applicants: Evaluation of Scores of Self-Reported Animal Experience and Rural Versus Urban Background.

Admission to veterinary school is generally based on academic and nonacademic measures. Descriptions of animal or veterinary experience and rural versus urban background are often sought from applicants, but little is objectively known about their impact on admission success or future performance. We evaluated scores from written descriptions from 590 veterinary school applicants for the nature and extent of self-reported animal experience. For those admitted to the program, we compared animal experience and rural versus urban background to performance in discipline-based courses, professional skills courses, clinical rotations, and the North American Veterinary Licensing Exam (NAVLE). More than 98% of applicants reported animal experience, with small animal veterinary experience most reported. There was no difference in animal experience or background between successful and unsuccessful applicants, but rural and urban applicants reported different experiences. There was a small correlation between small animal experience and performance in clinical rotations (.21), a small negative correlation between rural background and NAVLE performance (-.23), but otherwise, no significant correlations between animal experience or background and future performance. These findings suggest that scores of self-reported animal experience do not provide predictive information on applicants, or, alternatively, that the nature and extent of animal experience, the methods used to score these experiences, and/or the measures assessed during veterinary school need to be explicitly defined to ensure that we are capturing the appropriate information. More investigation into the scoring and impact of animal experience and background on applicant performance in the DVM program and success in a veterinary career is warranted.

The Formulation and Evaluation of Customized Prednisolone Gel Tablets Prepared by an Automated Extrusion-Based Material Deposition Method.

Background/Objectives: An automated extrusion-based material deposition is a contemporary and rapid method for pharmaceutical dose-dispensing and preparing (printing) individualized solid dosage forms. The aim of this study was to investigate and gain knowledge of the feasibility of automated extrusion-based material deposition technology in preparing customized prednisolone (PRD)-loaded gel tablets for veterinary applications (primarily for dogs and cats). Methods: The PRD loads of the extrusion-based deposited gel tablets were 0.5% and 1.0%, and the target weights of tablets were 0.250 g, 0.500 g, and 1.000 g. The effects of the material deposition processes on the physical solid state, in vitro dissolution, and the physicochemical stability of PRD gel tablets were investigated. Results: The small-sized gel tablets presented a uniform round shape with an exceptionally smooth outer surface texture. The actual average weight of the tablets (n = 10) was very close to the target weight, showing the precision of the process. We found that PRD was in a pseudopolymorphic sesquihydrate form (instead of an initial PRD crystalline form II) in the gel tablets. In all the immediate-release gel tablets studied, more than 70% of the drug load was released within 30 min. The soft texture and dimensions of gel tablets affected the dissolution behaviour in vitro, suggesting the need for further development and standardization of a dissolution test method for such gel tablets. A short-term storage stability study revealed that the content of PRD did not decrease within 3 months. Conclusions: Automated extrusion-based material deposition is a feasible method for the rapid preparation of gel tablets intended for veterinary applications. In addition, the present technology and gel tablets could be used in pediatric and personalized medicine where precise dosing is crucial.

Feline Infectious Peritonitis Effusion Index: A Novel Diagnostic Method and Validation of Flow Cytometry-Based Delta Total Nucleated Cells Analysis on the Sysmex XN-1000V®.

The emergence of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has led the medical and scientific community to explore the pathogenesis and clinical manifestations of coronaviruses. In felines, a widespread coronavirus known as feline coronavirus (FCoV) can lead to feline infectious peritonitis (FIP), a highly fatal disease characterised by severe systemic inflammation. Diagnosing FCoV remains challenging due to the limited accuracy of the available methods. The present study introduces the FIP Effusion Index, a novel diagnostic method that combines the albumin-to-globulin (ALB/GLOB) ratio with the delta total nucleated cell (∆TNC) count obtained via flow cytometry using the Sysmex XN-1000V® analyser in effusions. Samples from cats (n = 50) with suspected FIP were analysed for ∆TNC, with findings showing that a ∆TNC ≥ 2.1 is highly indicative of FIP and a ∆TNC ≥ 4.9 can be considered diagnostic. The FIP Effusion Index enhanced diagnostic precision in our group of samples, achieving 96.3% sensitivity and 95.7% specificity for values ≥ 5.06, and reaching perfect specificity (100%) with 96.3% sensitivity for values ≥ 7.54. This combined approach surpasses the accuracy of individual parameters, establishing the FIP Effusion Index as a superior diagnostic tool for FIP, with potential applications in both veterinary and human medicine for related coronavirus diseases.

Utilization of Computed Tomography at the Aydin Adnan Menderes University Faculty of Veterinary Medicine Research and Application Hospital: An Analysis of Preliminary Results on Patient Demographics and Case Distribution

This study examines the distribution of computed tomography (CT) usage at Aydin Adnan Menderes University Faculty of Veterinary Medicine Research and Application Hospital between 2023 and 2024. CT, a widely adopted diagnostic tool in veterinary medicine, allows for detailed imaging of internal organs and anatomical structures, enhancing the diagnosis of complex cases. The study analyzed data from 143 animals, including dogs, cats, and other species, to assess the frequency of CT scans based on species, age, sex, breed, and imaged anatomical regions. A total of 350 CT scans were reviewed, with dogs (n=87) undergoing the majority of scans (200), followed by cats (n=50) with 175 scans, and other species accounting for 26 scans. Cranium, thorax, and abdomen were the most frequently imaged regions across all species. The study found that older animals, particularly large-breed dogs and male cats, required more imaging, while younger animals (0-6 months) underwent fewer CT scans. The findings emphasize the need for tailored diagnostic approaches based on species, age, and sex to improve early detection and treatment outcomes in veterinary practice. Furthermore, the study highlights the potential for expanding CT use in non-traditional veterinary species, with the goal of improving diagnostic precision. The results provide important insights for enhancing veterinary services through the strategic application of CT imaging.

STEM CELL APPROACHES IN ORGAN REGENERATION AND REPAIR

Recent advances in stem cell research have provided new hope for treating a variety of diseases and conditions that currently have no effective cure. Stem cells have the unique ability to differentiate into various cell types, enabling them to support tissue growth and replace damaged or specialized cells throughout the lifespan. Among the most promising types of stem cells are mesenchymal stem cells (MSCs), which are easily harvested from adipose tissue and can be cultured and expanded in the lab. Due to their versatility, MSCs have become a focal point in tissue regeneration and have been widely used in animal studies and clinical trials involving humans. This review aims to summarize the current understanding of MSCs, focusing on the various types of stem cells isolated from different animal models such as horses, pigs, goats, dogs, rabbits, cats, rats, and mice. Given the growing interest in MSCs, we will also discuss their applications in veterinary and regenerative medicine. Current research highlights the potential of MSCs in treating conditions like heart failure, wound healing, and tooth regeneration, demonstrating their broad therapeutic potential. KEYWORDS: Mesenchymal stem cells (MSCs), animal models, cell-based therapy, regenerative medicine

Integrating AI into practice.

The possible veterinary applications of chatbots such as ChatGPT are already revealing themselves, but there are pitfalls to be aware of too.

Liposome-based drug and vaccine delivery system in veterinary application: recent advancement and future trends – a review

Abstract Liposomal technology has become a significant advancement in the field of veterinary therapeutics, offering increased performance and safety in the delivery of treatments such as antibiotics, anti-inflammatories, and anti-cancer medications. With their superior stability, improved encapsulation of drugs, and more efficient transport mechanisms, nanoparticles are becoming a key tool for future uses in veterinary applications. This review article explores the latest developments and potential future applications of liposomes for delivering drugs and vaccines in veterinary medicine. Furthermore, we delve into the potential of liposomes for ground-breaking applications. These include delivering drugs precisely to specific tissues and organs, alongside the incorporation of immunomodulatory agents to boost vaccine effectiveness in veterinary medicine. It will emphasize the critical role of liposomes in the administration of vaccines and pharmaceuticals, as well as their potential as nanoscale carriers in veterinary applications. It will highlight the expanding significance of liposomes in veterinary medicine and delve into their potential as a foundational technology for advancing animal health management strategies.

Advancements in genetic analysis: Insights from a case study and review of next-generation sequencing techniques for veterinary oncology applications.

Acute myeloid leukemia (AML) poses significant challenges in veterinary medicine, with limited treatment options and poor survival rates. While substantial progress has been made in characterizing human AML, translating these advancements to veterinary practice has been hindered by limited molecular understanding and diagnostic tools. The case study presented illustrates the application of whole genome sequencing in diagnosing AML in a dog, showcasing its potential in veterinary oncology. Our approach facilitated comprehensive genomic analysis, identifying mutations in genes that may be associated with AML pathogenesis in dogs, such as KRAS, IKZF1, and RUNX1. However, without supportive evidence of its clinical utility (eg, association with response to treatment or prognosis), the information is limited to exploration. This article reviews the comparative features of canine AML with human AML and discusses strategies to shrink the knowledge gap between human and veterinary medicine with cost-effective next-generation sequencing (NGS) techniques. By utilizing these approaches, the unique and shared molecular features with human AML can be identified, aiding in molecular classification and therapeutic development for both species. Despite the promise of NGS, challenges exist in implementing it into routine veterinary diagnostics. Cost considerations, turnaround times, and the need for robust bioinformatics pipelines and quality control measures must be addressed. Most importantly, analytical and clinical validation processes are essential to ensure the reliability and clinical utility of NGS-based assays. Overall, integrating NGS technologies into veterinary oncology holds great potential for advancing our understanding of AML and improving disease stratification, in hopes of improving clinical outcomes.

Comparison of academic achievement, graduation competency and academic sustainability of veterinary students based on college admission processes

A university’s admission policy is established to select applicants who can successfully complete the curriculum. Thus, it is important to develop optimal admissions processes for applicant selection. This study was conducted to infer the most suitable admission processes for veterinary school applicants by examining academic achievement, graduation competency and academic sustainability. It was conducted on students admitted from 2009 to 2018 at one of the veterinary colleges in Korea. The admission methods were divided into, first the regular admission (RA) process, primarily reflecting the results of the College Scholastic Ability Test, and second, the rolling admission processes. The rolling processes were further divided into admission officer-based (AO), high school grade point average-based (GA), essay test-based (ET), admission for the underprivileged (UP) and admission for overseas Koreans and foreigners (OKF). The final grade point average was observed to be higher in the order of the GA, AO and RA groups. The survey on the veterinary graduation competency did not reveal any difference between the regular and rolling admission processes. However, among the latter, AO-based admission showed a higher ratio of outstanding competency levels. In terms of academic sustainability, OKF admission demonstrated a higher rate of failure while RA showed a higher rate of expulsion and dropout. Summarizing the above results, the most successful veterinary applicants were selected through AO-based admission. These results are meaningful in providing the basic information required to establish admission policies for veterinary colleges in Korea.

One Hundred Years of Pyrethroid Chemistry: A Still-Open Research Effort to Combine Efficacy, Cost-Effectiveness and Environmental Sustainability

A century after the first scientific research on the chemical structures of pyrethrins was published (in 1923), this paper aims to provide an exhaustive review of the historical research pathways and relative turning points that led to the discovery and mass production of pyrethroids, which have become among the most commercially successful insecticides. These compounds, which are not specific to any particular pest, are used globally and offer cost-effective advantages against a broad spectrum of pests in both agricultural and non-agricultural situations. They are utilized in the context of both harvest and post-harvest applications, as well as in the implementation of public health programs and veterinary applications. Currently, the research for new pyrethroids has essentially reached a standstill due to the increasingly widespread occurrence of insecticide resistance in pests. Nevertheless, several research paths remain open regarding these pesticides. This paper represents the current state of knowledge regarding pyrethroids, exposing both their advantages and disadvantages. Moreover, further investigation, at the molecular level, on their mode of action (MoA) could be very useful to improve their specificity. The results of this review may stimulate additional research for the development of novel pyrethroids having enhanced efficacy, low cost and reduced environmental impact.

Some Aspects and Convergence of Human and Veterinary Drug Repositioning.

Drug innovation traditionally follows a de novo approach with new molecules through a complex preclinical and clinical pathway. In addition to this strategy, drug repositioning has also become an important complementary approach, which can be shorter, cheaper, and less risky. This review provides an overview of drug innovation in both human and veterinary medicine, with a focus on drug repositioning. The evolution of drug repositioning and the effectiveness of this approach are presented, including the growing role of data science and computational modeling methods in identifying drugs with potential for repositioning. Certain business aspects of drug innovation, especially the relevant factors of market exclusivity, are also discussed. Despite the promising potential of drug repositioning for innovation, it remains underutilized, especially in veterinary applications. To change this landscape for mutual benefits of human and veterinary drug innovation, further exploitation of the potency of drug repositioning is necessary through closer cooperation between all stakeholders, academia, industry, pharmaceutical authorities, and innovation policy makers, and the integration of human and veterinary repositioning into a unified innovation space. For this purpose, the establishment of the conceptually new "One Health Drug Repositioning Platform" is proposed. Oncology is one of the disease areas where this platform can significantly support the development of new drugs for human and dog (or other companion animals) anticancer therapies. As an example of the utilization of human and veterinary drugs for veterinary repositioning, the use of COX inhibitors to treat dog cancers is reviewed.

Quantitative estimation of doxylamine succinate by zero-order derivative area under curve Spectrophotometric methods in bulk and in-tablet dosage form

The purpose of this study is to establish Zero-order UV-Spectrophotometric - absorbance and Zero Order- Area under curve (AUC) methods for estimation of Doxylamine succinate in bulk and in pharmaceutical dosage form . Doxylamine succinate is Histamine H1 antagonist with pronounced sedative properties. It is used in allergies and as an antitussive, antiemetic, and hypnotic. Doxylamine has also been administered in veterinary applications and was formerly used in parkinsonism, distilled water was used as solvent for solubilization of Doxylamine succinate. Maximum absorption for Doxylamine succinate was found to be at wavelength 260nm when dissolved in Distilled water. The methods are based upon measurement of absorbance at 260nm and integration of area under curve for analysis of Doxylamine succinate in the wavelength range of 251.20-267.20 nm. The drug followed linearity in the concentration range of 10 - 60µg/mL with correlation coefficient value r&amp;#62; 0.99 . The proposed methods were validated for accuracy (% recovery), precision, repeatability and ruggedness, as per ICH guidelines. The proposed methods were applied for qualitative and quantitative estimation of Doxylamine succinate in tablet and results were found in good agreement with the label claimed. Developed methods can be used for routine analysis of Doxylamine succinate in bulk and pharmaceutical tablet.