At present, the prevalence of infectious diseases is rising annually, making it an important risk factor for human health that should not be neglected. Consequently, infection control and prevention have become even more important. The key to determining and designing the most effective anti-infectious medication depends upon the immediate and accurate identification of the causative agent. The standard techniques used for routine infection screening and surveillance tests are shifting toward biosensors. Furthermore, biosensors are projected to be employed for microbiological detection to satisfy the higher accuracy required for clinical diagnosis. This is because of their compact size, real-time monitoring and ability to analyze large sample numbers with less sophistication and manpower requirement, which have allowed them to develop quickly with extensive uses. Biosensors have multiple applications in food safety, environmental surveillance, drug sensing and national security because they offer several advantages such as quick response, outstanding sensitivity, remarkable selectivity, high degree of accuracy and precision, ease of use and affordable price. This review highlights the performance aspects of recently developed biosensors for the detection of infectious bacteria and viruses in biological and environmental samples and emphasizes the significance of nanotechnology in signal amplification for enhanced biosensor performance and dependability.