Er3+ doped tellurite fibers have been fabricated using a convenient suction technique. Different scanning calorimetry (DSC), Electro-probe micro-analyzer (EPMA), Raman, steady and dynamic luminescence spectra were performed to investigate their thermal, structure, and luminescent properties. Broadband 2.7 μm amplified spontaneous emission (ASE) emission with concurrent 1.5 μm and visible up-conversion emissions have been achieved in fibers with various lengths upon the excitation of 980 nm laser diode (LD). A quantitative study of the spectroscopic properties has been further analyzed in detail according to the Jude-Ofelt model, rate equation, and Dexter’s theory to evaluate the competitive relationship among them. The desirable spectroscopic characteristics associated with excellent thermal stability and mechanical properties indicate that the Er3+ doped tellurite fibers are excellent host matrices for 2.7 μm lasing and may provide applications in mid-infrared fiber lasers and amplifiers.