Drug Delivery Applications Research Articles

Hydrogel-Embedded Polydimethylsiloxane Contact Lens for Ocular Drug Delivery.

Topical administration is the commonly preferred method of administering ophthalmic formulations, with the majority of available medications in the form of eye drops or ointments. However, the topical application of ophthalmological medications has less bioavailability and a short residence time because of the physiological and anatomical constraints of the eye, making efficient ophthalmic drug delivery a challenging task. Microfluidic contact lenses have the advantage of delivering drugs into the eye in a controlled and on-demand manner. Here, we showcase the use of hydrogel-embedded microcavities on PDMS-based contact lenses for ocular drug delivery applications. The fabrication technique adopted here is the spontaneous formation of the spherical cavity by hydrogel monomer droplet, followed by the simultaneous thermal curing of hydrogel and PDMS, creating a spherical cavity as small as 150 μm. The spherical cavity is embedded with pH-responsive hydrogel for on-demand drug delivery. The drug loaded in the hydrogel matrix is released into the ocular environment by diffusion. The spherical cavity with a narrow opening restricts the diffusion to a minimum under normal ocular pH conditions(pH > 6). When the ocular pH reduces (pH < 6), the pH-responsive hydrogel inside the spherical cavity deswell and accelerates the drug release.

Distinct Solubilization Mechanisms of Medroxyprogesterone in Gemini Surfactant Micelles: A Comparative Study with Progesterone

The solubilization behavior of medroxyprogesterone (MP) within gemini surfactant micelles (14-6-14,2Br−) was investigated and compared with that of progesterone to uncover distinct solubilization mechanisms. We employed 1H-NMR and 2D ROESY spectroscopy to elucidate the spatial positioning of MP within the micelle, revealing that MP integrates more deeply into the micellar core. This behavior is linked to the unique structural features of MP, particularly its 17β-acetyl group, which promotes enhanced interactions with the hydrophobic regions of the micelle, while the 6α-methyl group interacts with the hydrophilic regions of the micelle. The 2D ROESY correlations specifically highlighted interactions between the hydrophobic chains of the surfactant and two protons of MP, H22 and H19. Complementary machine learning and electron density analyses supported these spectroscopic findings, underscoring the pivotal role of the molecular characteristics of MP in its solubilization behavior. These insights into the solubilization dynamics of MP not only advance our understanding of hydrophobic compound incorporation in gemini surfactant micelles but also indicate the potential of 14-6-14,2Br− micelles for diverse drug delivery applications.

Unveiling Fluorescence Spectroscopy, Molecular Docking and Dynamic Simulations: Interactions Between Protein and 2, 4-Dinitrophenylhydrazine Schiff Base.

In this study, we aimed to explore the interaction mechanism between bovine serum albumin (BSA) and a Schiff base compound derived from 2,4-dinotrophenyl hydrazine (L) using various spectroscopic techniques. The interaction between BSA and synthesizing molecule can provide insights into binding affinity, conformational changes and potential applications in drug delivery or biochemistry. The interaction between BSA and L was studied by using UV-Vis and fluorescence titration analysis. The fluorescence quenching emission was observed at 343nm, upon addition of L to the buffer solution of BSA. The binding between BSA and ligand is static in nature using fluorescence quenching emission. The thermodynamic parameters were calculated from the temperature-dependent binding constants (i.e., ∆H = -0.318kcal/mol, ∆G = -7.857kcal/mol and ∆S = 0.023kcal/mol), which indicated that the protein-ligand complex formation between L and BSA is mainly due to the electrostatic interactions. The experimental and theoretical results showed excellent agreement with respect to the mechanism of binding and binding constants. The molecular docking and molecular dynamic analysis experiments were performed to establish the interaction between protein and ligand.

Aminosilane@mesoporoussilica/chitosan∗salicylaldehyde nanohybrid: synthesis, characterization and applications

“Hydrogen-borrowing” mechanism has enables the production of biologically active molecules with minimal waste, high efficiency and mild reaction conditions. However, majority of research on the above mechanism has been focused on small molecule transformations. Hence, using Fe-tetraphenylcyclopentadienone tricarbonyl complex as a catalyst for “hydrogen-borrowing” mechanism between amines and alcohols, a novel pH-responsive inorganic-organic hybrid nanocarrier based on mesoporous silica-chitosan (MSN@CONH2/CS∗SCA) was developed to overcome these limitations. The nanocarrier was fabricated through C-N bond formation between 1-[3-trimethoxysiliylpropyl] urea functionalized mesoporous silica and salicylaldehyde modified chitosan derivative for antibacterial, antioxidant and drug delivery application. The chemical composition of synthesized nanocarrier was characterized by FTIR, 1H NMR and XRD techniques while the thermal stability examined through TGA. The surface morphology and porosity were evaluated using BET, SEM, and TEM whereas elemental composition, particle size and surface charge were determined by EDX with mapping, PSA and Zeta-potential method respectively. The nanohybrid was loaded with quercetin and curcumin drug, with 82.68 % and 83.24 % encapsulation efficiency respectively. The controlled drug release for quercetin was observed with 76 % (pH-5.0) and 20 % (pH-7.4), while for curcumin it observed 70.36 % (pH-5.0) and 19.16 % (pH-7.4) in 72 h. The antibacterial performance of nanocarrier shows better zone of inhibition for E. coli [2.9 ± 0.2 cm(3)] as compared to B. subtilis [1.6 ± 0.3 cm(3)]. The antioxidant ability of nano-hybrid to scavenge DPPH and ABTS radicals were 53.18 % and 55.21 %, respectively. Thereafter, MTT cytotoxicity assay of nanohybrid on peripheral blood mononuclear cells) exhibited promising results for biomedical applications.

Form Equals Function: Influence of Coacervate Architecture on Drug Delivery Applications.

Complex coacervates, formed through electrostatic interactions between oppositely charged polymers, present a versatile platform for drug delivery, providing rapid assembly, selective encapsulation, and responsiveness to environmental stimuli. The architecture and properties of coacervates can be tuned by controlling structural and environmental design factors, which significantly impact the stability and delivery efficiency of the drugs. While environmental design factors such as salt, pH, and temperature play a crucial role in coacervate formation, structural design factors such as polymer concentration, polymer structure, mixing ratio, and chain length serve as the core framework that shapes coacervate architecture. These elements modulate the phase behavior and material properties of coacervates, allowing for a highly tunable system. In this review, we primarily analyze how these structural design factors contribute to the formation of diverse coacervate architecture, ranging from bulk coacervates to polyion complex micelles, vesicles, and cross-linked gels, though environmental design factors are considered. We then examine the effectiveness of these architectures in enhancing the delivery and efficacy of drugs across various administration routes, such as noninvasive (e.g., oral and transdermal) and invasive delivery. This review aims to provide foundational insights into the design of advanced drug delivery systems by examining how the origin and chemical structure of polymers influence coacervate architecture, which in turn defines their material properties. We then explore how the architecture can be tailored to optimize drug delivery for specific administration routes. This approach leverages the intrinsic properties derived from the coacervate architecture to enable targeted, controlled, and efficient drug release, ultimately enhancing therapeutic outcomes in precision medicine.

Conformations of α-cyclodextrin and its role on the stability of inclusion complexes in aqueous solution

α-Cyclodextrin (αCD), a cyclic carbohydrate polymer, presents a wealth of opportunities for drug delivery and various industrial applications. Its high affinity and selectivity in encapsulating target molecules have been well-documented. However, the influence of αCD's conformational variations on its encapsulation capacity remains a promising area for further exploration. This study explores the conformational dynamics of αCD and its relationship with the formation of inclusion complexes (ICs) in aqueous solutions, focusing on understanding the forces driving these dynamics and the formation of stable ICs. Classical molecular dynamics (CMD) demonstrate that αCD solvated in water can adopt two conformations: a truncated cone shape with a suitable cavity for encapsulation and a distorted cone shape with a collapsed cavity. The predominant conformation observed in the CMD trajectory of pristine αCD is the distorted cone. However, under complexation conditions with aliphatic molecules, the guest molecule's size dictates the ICs' stability, forcing αCD to maintain the truncated cone conformation. Quantum chemistry methods and electron density analyses (Quantum Theory of Atoms in Molecules and Non-Covalent Interactions index) support CMD observations by characterizing and quantifying non-covalent interactions. Findings indicate that the shift from the truncated to the distorted cone conformation in αCD is primarily driven by intramolecular non-covalent interactions and the reconfiguration of water molecules surrounding αCD. The ICs' analysis displays a significant correlation between the size of the aliphatic chain and the intensity of intermolecular interactions. The strength of these interactions can effectively disrupt the αCD intramolecular non-covalent interactions that lead to a conformational change and, consequently, the loss of the inclusion complex. In conclusion, although αCD's conformational change can affect encapsulation, guest molecules can manipulate the conformational preference. The systematic analysis of non-covalent interactions offers a way to characterize and quantify these forces, enhancing our ability to understand and predict the stability of inclusion complexes.

Mechanistic Model for Drug Release from PLGA-Based Biodegradable Implants for In Vitro Release Testing: Development and Validation.

Several factors can affect drug release from polylactide coglycolide (PLGA)-based formulations, including polymer and drug properties, formulation components, manufacturing processes, and environmental in vitro or in vivo conditions. To achieve optimal release profiles for specific drug delivery applications, it is crucial to understand the mechanistic processes that determine drug release from PLGA-based formulations. In the current study, we developed a mechanistic model for the in vitro drug release of PLGA-based solid implants. The model accounts for all known critical quality attributes (CQAs) and considers the most important release rate processes, including water or dissolution medium influx into the porous structure of the implant, initial noncatalytic hydrolysis of PLGA, autocatalytic hydrolysis, dissolution of oligomers and monomers into the aqueous medium, the liberation of the trapped solid drug from the polymer matrix, dissolution of the solid drug into the wetted pore network, diffusion of the dissolved drug out of the implant, and distribution of the dissolved drug into the dissolution medium. The model has been validated using in vitro release data obtained from implants of four drugs (buserelin, afamelanotide, brimonidine, and nafarelin). The model presented in this manuscript provides valuable insights into the kinetics and mechanism of drug release from PLGA-based solid implants and has demonstrated the potential for optimizing formulation design. The in vitro release model, coupled with physiologically based pharmacokinetic (PBPK) modeling, can predict the in vivo performance of implants and can be used to support bioequivalence studies in a drug development program.

Review of Gold Nanoparticles: Synthesis, Properties, Shapes, Cellular Uptake, Targeting, Release Mechanisms and Applications in Drug Delivery and Therapy

The remarkable versatility of gold nanoparticles (AuNPs) makes them innovative agents across various fields, including drug delivery, biosensing, catalysis, bioimaging, and vaccine development. This paper provides a detailed review of the important role of AuNPs in drug delivery and therapeutics. We begin by exploring traditional drug delivery systems (DDS), highlighting the role of nanoparticles in revolutionizing drug delivery techniques. We then describe the unique and intriguing properties of AuNPs that make them exceptional for drug delivery. Their shapes, functionalization, drug-loading bonds, targeting mechanisms, release mechanisms, therapeutic effects, and cellular uptake methods are discussed, along with relevant examples from the literature. Lastly, we present the drug delivery applications of AuNPs across various medical domains, including cancer, cardiovascular diseases, ocular diseases, and diabetes, with a focus on in vitro and in vivo cancer research.

Point-Spread Function Deformations Unlock 3D Localization Microscopy on Spherical Nanoparticles.

Nanoparticles (NPs) have proven their applicability in biosensing, drug delivery, and photothermal therapy, but their performance depends critically on the distribution and number of functional groups on their surface. When studying surface functionalization using super-resolution microscopy, the NP modifies the fluorophore's point-spread function (PSF). This leads to systematic mislocalizations in conventional analyses employing Gaussian PSFs. Here, we address this shortcoming by deriving the analytical PSF model for a fluorophore near a spherical NP. Its calculation is four orders of magnitude faster than numerical approaches and thus feasible for direct use in localization algorithms. We fit this model to individual 2D images from DNA-PAINT experiments on DNA-coated gold NPs and demonstrate extraction of the 3D positions of functional groups with <5 nm precision, revealing inhomogeneous surface coverage. Our method is exact, fast, accessible, and poised to become the standard in super-resolution imaging of NPs for biosensing and drug delivery applications.

Synthesis, Urease Inhibition, Molecular Docking, and Optical Analysis of a Symmetrical Schiff Base and Its Selected Metal Complexes.

Designing and developing small organic molecules for use as urease inhibitors is challenging due to the need for ecosystem sustainability and the requirement to prevent health risks related to the human stomach and urinary tract. Moreover, imaging analysis is widely utilized for tracking infections in intracellular and in vivo systems, which requires drug molecules with emissive potential, specifically in the low-energy region. This study comprises the synthesis of a Schiff base ligand and its selected transition metals to evaluate their UV/fluorescence properties, inhibitory activity against urease, and molecular docking. Screening of the symmetrical cage-like ligand and its metal complexes with various eco-friendly transition metals revealed significant urease inhibition potential. The IC50 value of the ligand for urease inhibition was 21.80 ± 1.88 µM, comparable to that of thiourea. Notably, upon coordination with transition metals, the ligand-nickel and ligand-copper complexes exhibited even greater potency than the reference compound, with IC50 values of 11.8 ± 1.14 and 9.31 ± 1.31 µM, respectively. The ligand-cobalt complex exhibited an enzyme inhibitory potential comparable with thiourea, while the zinc and iron complexes demonstrated the least activity, which might be due to weaker interactions with the investigated protein. Meanwhile, all the metal complexes demonstrated a pronounced optical response, which could be utilized for fluorescence-guided targeted drug delivery applications in the future. Molecular docking analysis and IC50 values from in vitro urease inhibition screening showed a trend of increasing activity from compounds 7d to 7c to 7b. Enzyme kinetics studies using the Lineweaver-Burk plot indicated mixed-type inhibition against 7c and non-competitive inhibition against 7d.

A comprehensive review on the classification, synthesis, characterization of nanopartices and their therapeutic impact

The main topic of the document is nanotechnology and the use of nanoparticles in various fields. The document introduces nanotechnology and defines nanoparticles as particles with lengths between 1 nm and 100 nm. Nanoparticles are classified as zero-dimensional nanomaterials and have distinct physico-chemical characteristics compared to bulk materials due to their small size and high surface to volume ratio. Nanoparticles have gained attention in technological breakthroughs due to their adjustable properties and potential applications in drug delivery. The document also discusses the different types of nanoparticles, including organic, inorganic, carbon-based, and herbal nanoparticles. Organic nanoparticles, such as micelles and liposomes, are often used for drug delivery. Inorganic nanoparticles, such as silver and gold nanoparticles, find applications in cosmetics and medicine. Carbon-based nanoparticles, including graphene and carbon nanotubes, have various uses in fields such as energy storage and sensors. The document also mentions the use of herbal nanoparticles derived from plant extracts. Additionally, the document highlights the concept of green synthesis, which offers advantages in terms of environmental friendliness and sustainability. Overall, the review provides an overview of the significance and potential applications of nanoparticles in different industries.

Precision Design of Sequence‐Defined Polyurethanes: Exploring Controlled Folding Through Computational Design

AbstractThis study presents the exploration of sequence‐defined polyurethanes (PUs) as a new class of heteropolymers capable of precise conformational control. Utilizing molecular dynamics simulations, the folding behavior of polyurethane chains is investigated of varying lengths (11, 20, and 50 monomers) in both vacuum and aqueous environments. The simulations reveal that the heterogeneous chains systematically refold to approach the designed target structures better than non‐designed chains or chains with artificially disrupted hydrogen‐bond networks. The subsequent synthesis of an optimized 11‐mer sequence (P1) is achieved through solid‐phase chemistry, with thorough characterization via NMR, MS, and SEC confirming the accuracy of the predicted sequence and its controlled chain length. Solubility tests showed favorable results across multiple solvents, highlighting the versatility of the designed polymer. This research underscores the potential of sequence‐defined polyurethanes to emulate the structural and functional attributes of biological macromolecules, opening new pathways for their application in catalysis, drug delivery, and advanced material design. The findings illustrate a promising direction for the development of synthetic polymers with tailored properties, emphasizing the transformative impact of sequence control in polymer chemistry.

Curcumin-loaded zein nanoparticles: A quality by design approach for enhanced drug delivery and cytotoxicity against cancer cells

Zein, a maize protein, has been explored for constructing potential biomaterial due to its hydrophobic nature, self-assembly capability, and biocompatibility. In its nanoparticulate form, zein is a promising material for drug delivery applications, particularly in cancer treatment. Despite the importance of colloidal stability for effective drug delivery, systematic studies investigating the effect of various surface modifying agents (MAs) on the zein nanoparticles (ZNPs)-based formulations are limited. This study employs quality-by-design (QbD) approach to optimize curcumin-loaded ZNPs, enhancing colloidal stability, size, and drug-encapsulation efficiency using different MAs for potential cancer therapy. Gum arabic (GA) emerged as the optimal stabilizer, with GA-stabilized curcumin-loaded ZNPs (GA-Cur-ZNPs) achieving a particle size of 184.8 ± 2.85 nm, zeta potential of −23.4 ± 0.56 mV and 87.1 ±1.55 % drug encapsulation efficiency, along with excellent colloidal stability over two months. The optimal formulation also demonstrated sustained release of Cur over 72 h. GA-Cur-ZNPs demonstrated lower IC50 values and higher anti-proliferative effects on three different cancer cell lines compared to the free drug, while also exhibiting superior intracellular uptake. With negligible toxicity to human dermal fibroblast cells, the optimized Cur-GA-ZNPs show promise for safe and effective killing of cancer cells.

Synthesis, Characterization and in Vitro Drug Delivery Applications of a Tin-Based Metal‒Organic Framework

Synthesis, Characterization and in Vitro Drug Delivery Applications of a Tin-Based Metal‒Organic Framework

Berberine-Loaded Bovine Serum Albumin Nanoparticles: Fabrication, Characterization, and Drug Release Evaluation

Abstract Introduction/Objective Introduction: Numerous studies have highlighted the diverse biochemical and pharmacological properties of berberine (BER), including its anti-inflammatory, antioxidant, anticancer, and hypolipidemic effects. Nanoparticles play a crucial role in enhancing drug delivery by providing a larger surface area, facilitating quicker dissolution in the bloodstream. Methods/Case Report Methods: Blank bovine serum albumin nanoparticles (BSA NPs) were prepared using a desolvation process, followed by the fabrication of berberine-loaded BSA nanoparticles (BER-NP). Physicochemical characterization, including particle size and zeta potential, was conducted using a Malvern Zetasizer. The mean particle size and distribution were determined using photon correlation spectroscopy (PCS), and transmission electron microscope (TEM) images were obtained for visualization. Fourier transform infrared (FTIR) spectra and differential scanning calorimetry (DSC) thermograms were recorded to assess drug encapsulation efficiency (EE). Berberine release from BER-NPs was evaluated using dialysis membranes. Results (if a Case Study enter NA) Results: FTIR spectra and DSC thermograms confirmed successful drug loading into the nanoparticles. Encapsulation efficiency was determined by UV absorption spectroscopy, revealing high efficiency in the encapsulation process. Dialysis membranes demonstrated controlled release kinetics of berberine from BER-NPs, indicating their potential for targeted drug delivery. Conclusion Conclusion: Berberine-loaded bovine serum albumin nanoparticles exhibit favorable characteristics for drug delivery applications, including enhanced permeation across biological barriers. These nanoparticles hold promise for targeted therapy and combination drug regimens, offering potential benefits for various medical interventions.

Hydrolytically Degradable Zwitterionic Polyphosphazene Containing HEPES Moieties as Side Groups.

Zwitterionic polymers, ampholytic macromolecules containing ionic moieties of opposite sign on the same pendant groups, exhibit strong protein-repulsive properties and an inherent biological inertness. For that reason, these highly hydrated inner salt macromolecules have emerged as some of the most viable alternatives to poly(ethylene glycol) (PEG), a gold standard in enabling stealth behavior in life science applications. However, the structural diversity of polymer zwitterions remains limited, and currently available macromolecules do not possess an intrinsic ability to undergo hydrolytical degradation, an important prerequisite for use in drug delivery applications. The present paper reports on the synthesis of a zwitterionic polymer, a multimerized form (two thousand copies), of a biologically benign buffering agent, HEPES, which is covalently assembled on a polyphosphazene backbone. The polymer exhibits typical polyzwitterionic solution behavior, an environmentally dependent hydrolytic degradation pattern, and excellent in vitro compatibility, features that highlight its potential utility for life science applications.

Artificial Intelligence (AI) Applications in Drug Discovery and Drug Delivery: Revolutionizing Personalized Medicine

Artificial intelligence (AI) encompasses a broad spectrum of techniques that have been utilized by pharmaceutical companies for decades, including machine learning, deep learning, and other advanced computational methods. These innovations have unlocked unprecedented opportunities for the acceleration of drug discovery and delivery, the optimization of treatment regimens, and the improvement of patient outcomes. AI is swiftly transforming the pharmaceutical industry, revolutionizing everything from drug development and discovery to personalized medicine, including target identification and validation, selection of excipients, prediction of the synthetic route, supply chain optimization, monitoring during continuous manufacturing processes, or predictive maintenance, among others. While the integration of AI promises to enhance efficiency, reduce costs, and improve both medicines and patient health, it also raises important questions from a regulatory point of view. In this review article, we will present a comprehensive overview of AI’s applications in the pharmaceutical industry, covering areas such as drug discovery, target optimization, personalized medicine, drug safety, and more. By analyzing current research trends and case studies, we aim to shed light on AI’s transformative impact on the pharmaceutical industry and its broader implications for healthcare.

Stability Assessment in Aqueous and Organic Solvents of Metal-Organic Framework PCN 333 Nanoparticles through a Combination of Physicochemical Characterization and Computational Simulations.

Mesoporous metal-organic frameworks (MOFs) have been recognized as powerful platforms for drug delivery, especially for biomolecules. Unfortunately, the application of MOFs is restricted due to their relatively poor stability in aqueous media, which is crucial for drug delivery applications. An exception is the porous coordination network (PCN)-series (e.g., PCN-333 and PCN-332), a series of MOFs with outstanding stability in aqueous media at the pH range from 3 to 9. In this study, we fabricate PCN-333 nanoparticles (nPCN) and investigate their stability in different solvents, including water, ethanol, and methanol. Surprisingly, the experimental characterizations in terms of X-ray diffraction, Brunauer-Emmett-Teller (BET), and scanning electron microscopy demonstrated that nPCN is not as stable in water as previously reported. Specifically, the crystalline structure of nPCN lost its typical octahedral shape and even decomposed into an irregular amorphous form when exposed to water for only 2 h, but not when ethanol and methanol were used. Meanwhile, the porosity of nPCN substantially diminished while being exposed to water, as demonstrated by the BET measurement. With the assistance of computational simulations, the mechanism behind the collapse of PCN-333 is illuminated. By molecular dynamics simulation and umbrella sampling, we elucidate that the degradation of PCN-333 occurs by hydrolysis, wherein polar solvent molecules initiate the attack and subsequent breakage of the metal-ligand reversible coordination bonds.

Exploring Innovations in Transdermal Drug Delivery: Microneedle Technologies and the Latest in Patent Developments

Introduction/Objective: Microneedle technology has emerged as a promising approach for drug delivery, offering advantages such as improved patient compliance and enhanced therapeutic efficacy. This review aims to provide a comprehensive overview of recent advancements in microneedle- based drug delivery systems, emphasizing their potential to overcome limitations associated with traditional transdermal drug delivery methods. The objective is to synthesize existing knowledge, identify key trends, and highlight potential applications of microneedle technology in various medical fields. Methods: A systematic approach was employed to select and analyse relevant studies on microneedle technology. Databases were searched for peer-reviewed articles published and patents or patent applications worldwide within the last decade, focusing on innovations in microneedle materials, design, fabrication techniques, and applications. Studies were evaluated based on their methodology, outcomes, and relevance to current trends in drug delivery. Key data were extracted and synthesized to provide an integrated perspective on the state of microneedle technology. Results: The review highlights significant progress in microneedle technology; innovations in materials, fabrication techniques, and applications. Advancements include biodegradable microneedles, vaccine drug delivery systems, and integration with biosensors. Innovations led to improved drug bioavailability and reduced side effects. Challenges such as scalability, standardization, and regulatory considerations were also identified. Conclusion: In conclusion, microneedle technology has evolved significantly, offering a versatile platform for controlled drug delivery and various medical applications. The diversity in design and fabrication methods allows customization for specific therapeutic or diagnostic needs, with numerous patents filed for microneedle innovations, reflecting the intense research and development in this field.

Microbial Polysaccharides as Functional Components of Packaging and Drug Delivery Applications.

This review examines microbial polysaccharides' properties relevant to their use in packaging and pharmaceutical applications. Microbial polysaccharides are produced by enzymes found in the cell walls of microbes. Xanthan gum, curdlan gum, pullulan, and bacterial cellulose are high-molecular-weight substances consisting of sugar residues linked by glycoside bonds. These polysaccharides have linear or highly branched molecular structures. Packaging based on microbial polysaccharides is readily biodegradable and can be considered as a renewable energy source with the potential to reduce environmental impact. In addition, microbial polysaccharides have antioxidant and prebiotic properties. The physico-chemical properties of microbial polysaccharide-based films, including tensile strength and elongation at break, are also evaluated. These materials' potential as multifunctional packaging solutions in the food industry is demonstrated. In addition, their possible use in medicine as a drug delivery system is also considered.