Application Of Zinc Oxide Nanoparticles Research Articles

The Exposure and Hazards of Zinc Oxide Nanoparticles: In Vitro and In Vivo Studies

Background: Extensive application of zinc oxide nanoparticles has increased the likelihood of its release into the environment and subsequent human exposure and toxicity. The toxicity is thought to be a combined effect of intracellular particles and the release of dissolved zinc ions. Objectives: This review outlines the possible mechanisms of zinc oxide toxicity in biological organs through in vitro and in vivo experiments. Methods: We reviewed articles published between 2001 and 2021. In this way, we did a manual search of Google Scholar and scientific databases, including PubMed, Web of Science, Scopus, and Embase, with keywords such as “zinc oxide nanoparticles”, “toxicity mechanism”, and “in vivo and in vitro studies”. The other qualified papers contained the history of identifying zinc oxide nanoparticles, the toxicity of metallic nanoparticles, and physical, chemical, and biological side effects with topical and systematic approaches. Results: The main mechanism suggested for zinc-based nanoparticles-induced cell damage is via the induction of increased levels of reactive oxygen species, which are oxidative stress markers. This mechanism has also been found to be a key mechanism for the cytotoxicity of other metal nanomaterials. Zinc-based nanoparticles were found to induce oxidative DNA damage, inflammation, progressive degenerative cell changes, cell cycle arrest, cytogenetic alterations, and ROS-triggered mitochondria-mediated apoptosis in human organs. Conclusion: This review sheds light on the full understanding of in vitro and in vivo toxicity assessment of zinc oxide nanoparticles, highlighting the health concerns from the perspective of ZnO nanoparticles release to the ecosystem after their increasing application.

Responses of two strawberry cultivars to NaCl-induced salt stress under the influence of ZnO nanoparticles

Salinity stress is one of the most serious impacts of climate changes on agriculture production, especially in salt sensitive crop plants, like strawberry. Currently, the utilization of nanomolecules in agriculture is thought to be a useful strategy to compact abiotic and biotic stresses. This study aimed to investigate the effect of zinc oxide nanoparticles (ZnO-NPs) on the in vitro growth, ions uptake, biochemical and anatomical responses of two strawberry cvs (Camarosa and Sweet Charlie) under NaCl-induced salt stress. A 2x3x3 factorial experiment was conducted, with three levels of ZnO-NPs (0, 15 and 30 mg 1-l) and three levels of NaCl-induced salt stress (0, 35 and 70 mM). The results showed that increased levels of NaCl in the medium had led to decrease in shoot fresh weight and proliferative potential. The cv Camarosa was found to be relatively more tolerant to salt stress. Additionally, salt stress leads to an accumulation of toxic ions (Na + and Cl-), as well as a decrease in K + uptake. However, application of ZnO-NPs at a concentration of 15 mg 1-l was found to alleviate these effects by increasing or stabilizing growth traits, decreasing the accumulation of toxic ions and the Na+/K + ratio, and increasing K + uptake. Additionally, this treatment led to elevated levels of catalase (CAT), peroxidase (POD) and proline content. The positive impacts of ZnO-NPs application were reflected on the leaf anatomical features, being better adapted to salt stress. The study highlighted the efficiency of utilizing tissue culture technique in screening of strawberry cultivars for salinity tolerance under the influence of NPs.

Plant-ZnO nanoparticles interaction: An approach to improve guinea grass (Panicum maximum) productivity and evaluation of the impacts of its ingestion by freshwater teleost fish

We aimed to evaluate the possible effects of the application of zinc oxide nanoparticles [ZnO NPs; 68.96 ± 33.71 nm; at 100 and 500 mg/kg in a soil mixture of the Typic Dystrophic Red Latosol type and sand (2:1 ratio)] in the cultivation of Panicum maximum (until 125 days), using different biomarkers in addition to evaluating the uptake of Zn by the plants. Furthermore, we assessed the possible transfer of ZnO NPs from P. maximum leaves to zebrafish and their potential. Plants cultivated in substrates with ZnO NPs at 500 mg/kg showed reduced germination rate and growth. However, at 100 mg/kg, plants showed higher biomass and productivity, associated with higher Zn uptake, without inducing oxidative and nitrosative stress. Zinc content in zebrafish was not associated with ingesting leaves of P. maximum cultivated in substrate containing ZnCl2 or ZnO NPs or with genotoxic, mutagenic, and biochemical effects. In conclusion, ZnO NPs (at 100 mg/kg) are promising in the cultivation of P. maximum, and their ingestion by zebrafish did not cause changes in the evaluated biomarkers. However, we recommend that studies with other animal models be conducted to comprehensively assess the ecotoxicological hazard associated with applying ZnO NPs in soil.

Effect of Turmeric (Curcuma longa) Extract with Zinc Oxide Nanoparticles Application on Burn Wound

Effect of Turmeric (Curcuma longa) Extract with Zinc Oxide Nanoparticles Application on Burn Wound

Nano‐Biofortification of Zinc in Potato (Solanum tuberosum L.) and Tomato (Solanum lycopersicum L.) Crops

The present study is carried out to establish the biofortification of zinc in potato (Solanum tuberosum L.) and tomato (Solanum lycopersicum L.) plants through the application of zinc oxide nanoparticles (ZnONPs). The mycogenic ZnONPs are synthesized by using Aspergillus flavus biomass and characterized to ascertain the size, shape, and concentration of synthesized nanoparticles. The ZnONPs are applied by foliar spray to the field-grown potato and tomato crops and the biofortification level is recorded by Atomic Absorption Spectroscopy (AAS). A nanotoxicity assessment is done by comparing the amount of cell death and accumulation of reactive oxygen species (ROS) in control and ZnONPs treated plants. The AAS analysis reveals significant accumulation of the zinc in leaves of potato plants but insignificant accumulation is observed in the tubers. However, a significant accumulation of zinc is observed in the leaves and fruits of the tomato plants (p ≤ 0.05). The nanotoxicity evaluation studies show comparable and statistically insignificant toxicity in control plants as compared to plants treated with ZnONPs. The study concludes that biofortification through the means of ZnONPs is a cost-effective and eco-friendly approach that can be used as an alternative to the hazardous chemical zinc fertilizers.

A Novel Application of Zinc Oxide Nanoparticles for Inhibition of Molluscum contagiosum Virus Infection.

Molluscum contagiosum virus (MCV) is an infection caused by the molluscum contagiosum virus. Antiviral medications used to treat MCV infections have several problems, including drug-resistant and toxicity. As a result, improving safe, innovative, and effective antiviral drugs is critical. Therefore the current study aimed to investigate ZnO-NPs effects on M. contagisum infection and molluscum contagiosum virus replication, among the main exciting viruses that menace human health. The antiviral activity of zinc oxide nanoparticles (ZnO-NPs) against MCV infection was investigated in this work. FESEM and TEM electron microscopy were used to examine the nanoparticles. The cytotoxicity of the nanoparticles was assessed using the MTT assay, and anti-influenza effects were detected using RT-PCR and TCID50. An indirect immunofluorescence experiment was used to investigate the inhibitory effect of nanoparticles on viral antigen expression. In all tests, acyclovir was employed as a control. Compared to virus control, post-exposure of MCV with ZnO nanoparticles at the highest dose but is not toxic (100 g/mL) resulted in 0.2, 0.9, 1.9, and 2.8 log10 TCID50 reductions in infectious diseases virus titer (P=0.0001). This ZnO-nanoparticles level was accompanied by an inhibition percentage (17.8%, 27.3%, 53.3%, 62.5 %, and 75.9%), respectively, measured based on viral load compared with the virus control. Compared to the positive control, fluorescence emission intensity in virally infected cells that administrated ZnO nanoparticles was statically decreased. Our findings demonstrated that ZnO-NPs have antiviral effects against the MCV. This property indicates that ZnO-NP has a high potential for usage in topical formulations to treat facial and labial lesions.

A Comprehensive Review on Synthesis and Application of Zinc Oxide Nanoparticles

ZnO which are used as nanoparticles have been used in many varieties of paint, beautifiers, elastics, and covering. Just because of its biocompatibility, low toxicity as well as economic viability which are known as its exceptional cases, it has been the most used nanoparticle of metal oxides in many applications for the last twenty years. It can release particles of zinc, ability to induce cell death as well as the ability for producing oxygen species in excessive amounts. Due to its vast application in technology, it is also used in applications of biomedicines such as anticancer as well as antibacterial. Zinc also play important role in maintaining insulin credibility. Nanoparticles of zinc have been widely used for diabetes treatment. Additionally, it has been exceptionally dazzling applications that have leading them to be a candidate for use in bio imaging. Here, we are going to summarize the synthesis as well as continuing developments of nanoparticles of ZnO in the sector of biomedical, which may useful for fostering more advancement in the field of medicine and focusing on the areas of biomedical.

Impact of Foliar Application of Zinc and Zinc Oxide Nanoparticles on Growth, Yield, Nutrient Uptake and Quality of Tomato

Appropriate foliar application of zinc (Zn) and zinc oxide nanoparticles (ZnO-NPs) is important for the proper growth and yield of tomato. However, the effects of foliar application of Zn and ZnO-NPs were not well-studied on tomato production. A pot experiment was conducted at glasshouse (8D) conditions under the Faculty of Agriculture, Universiti Putra Malaysia (UPM) to evaluate the effectiveness of Zn and ZnO-NPs on growth, yield, nutrient uptake, and fruit quality of tomatoes and to compare between the Zn nutrient and ZnO-NPs. Treatment combinations were 14 viz. T1 = 0 (control), T2 = 1500 ppm (mg/L) Zn nutrient, T3 = 2000 ppm (mg/L) Zn nutrient, T4 = 2500 ppm (mg/L) Zn nutrient, T5 = 75 ppm ZnO nanoparticle, T6 = 100 ppm ZnO nanoparticle, and T7 = 125 ppm ZnO nanoparticle along with two tomato varieties. The experimental design was a split plot with four replications. Results indicated that foliar application of 100 ppm ZnO-NPs performed best in terms of growth parameters, physiological traits, yield attributes, yield, and quality traits of tomatoes. The same treatment (100 ppm ZnO-NPs) contributed to attain the highest nutrient uptake. Recovery use efficiency of Zn was highest with foliar application of 75 ppm ZnO-NPs. The highest yield increment (200%) over control was from foliar sprayed with 100 ppm ZnO-NPs. Comparing the two varieties, MARDI Tomato-3 (MT3) showed better than MARDI Tomato-1 (MT1). As is appears from the results, foliar application of zinc oxide nanoparticles was more efficient than conventional zinc fertilizer. Therefore, the foliar sprayed with 100 ppm ZnO-NPs can be suggested to improve quantity and quality of tomato in glasshouse soil conditions.

Zinc Oxide Nanoparticles in Alleviation of Toxicity Induced by Heat Stress in Plants

Climate change-related abiotic pressures have an impact on plant development and yield, which decreases the amount of food produced. High temperature (HT) stress has a large worldwide impact on plant development, metabolism, and productivity. The growth and development of plants involves numerous temperature-sensitive metabolic processes. The widespread practices of producing crops in polluted surroundings is currently environmentalists' top worry. So, in order to maintain high crop yields under HT stress, which is currently a key worry for crop production, it is vital for agriculture to develop various strategies. A current relevant technology for enhancing food production and ensuring sustainability in the pursuit of food safety is highly developed nanotechnology. Nanotechnology boosts agricultural productivity by improving input effectiveness and reducing relevant losses. This review looks at earlier studies that show how zinc oxide nanoparticles (ZnO-NPs) can mitigate the negative effects of heat stress. ZnO-NPs application is the most effective method available globally to greatly boost agricultural output in challenging settings. With the aid of a number of cutting-edge technologies for reversing the symptoms of oxidative stress brought on by heat stress, ZnO-NPs can revolutionize the agricultural and food industries. The impact of ZnO-NPs on the physiological, antioxidative and biochemical activities in different plants has also been thoroughly studied. This review summarizes the current understanding and future perspectives of plant-ZnO-NPs research.

Characterizing stomatal attributes and photosynthetic induction in relation to biochemical changes in Coriandrum sativum L. by foliar-applied zinc oxide nanoparticles under drought conditions.

Abiotic stress, particularly drought, will remain an alarming challenge for sustainable agriculture. New approaches have been opted, such as nanoparticles (NPs), to reduce the negative impact of drought stress and lessen the use of synthetic fertilizers and pesticides that are an inevitable problem these days. The application of zinc oxide nanoparticles (ZnO NPs) has been recognized as an effective strategy to enhance plant growth and crop production during abiotic stress. The aim of the current study was to investigate the role of ZnO NPs in drought stress management of drought-susceptible Coriandrum sativum L. (C. sativum) in two consecutive seasons. Drought regimes (moderate drought regime-MDR and intensive drought regime-IDR) were developed based on replenishment method with respect to 50% field capacity of fully irrigated (control) plants. The results showed that foliar application of 100 ppm ZnO NPs improved the net photosynthesis (Pn), stomatal conductance (C), and transpiration rate (E) and boosted up the photosynthetic capacity associated with photosynthetic active radiation in MDR. Similarly, 48% to 30% improvement of chlorophyll b content was observed in MDR and onefold to 41% in IDR during both seasons in ZnO NP-supplemented plants. The amount of abscisic acid in leaves showed a decreasing trend in MDR and IDR in the first season (40% and 30%) and the second season (49% and 33%) compared with untreated ZnO NP plants. The ZnO NP-treated plants showed an increment in total soluble sugars, total phenolic content, and total flavonoid content in both drought regimes, whereas the abaxial surface showed high stomatal density and stomatal index than the adaxial surface in foliar-supplied NP plants. Furthermore, ZnO NPs improve the magnitude of stomata ultrastructures like stomatal length, stomatal width, and pore length for better adaptation against drought. Principal component analysis revealed the efficacy of ZnO NPs in inducing drought tolerance in moderate and intensive stress regimes. These results suggest that 100 ppm ZnO NPs can be used to ameliorate drought tolerance in C. sativum plants.

Synthesis, Characterization, and Biomedical Applications of Zinc Oxide Nanoparticles

Synthesis, Characterization, and Biomedical Applications of Zinc Oxide Nanoparticles

The antioxidant and anti-apoptotic properties of vitamins A, C and E in heart tissue of rats exposed to zinc oxide nanoparticles.

The rapidly increasing applications of zinc oxide nanoparticles (ZnO NPs) in various industries have led to growing concerns about their damaging influence on human health. The present research was designed to determine the protective action of vitamins (Vits) A, C and E on the heart toxicity induced by ZnO NPs. Fifty-four male Wistar rats were allocated into 9 groups of 6 and then exposed to ZnO NPs (200mg/kg), water (Control1), olive oil (Control2), Vit A (1000 IU/kg), Vit C (200mg/kg), Vit E (100 IU/kg) and three groups were co-treated with ZnO and one of the Vits A, C or E. The oxidative stress situation was evaluated by measuring oxidative stress markers and the tissue antioxidant enzyme activity. Besides, the mRNA expression of Bcl-2 and Bax and caspase 3,7 activity were assessed. A histopathological examination was also performed to determine the rate of cardiac injury. The results indicated that co-administration of ZnO NPs and the aforementioned Vits significantly reduced the total oxidant status and lipid peroxidation relative to the ZnO group (P < 0.05). Furthermore, the supplementation of vitamins, notably Vit E, decreased the ZnO NPs-induced oxidative damage by enhancing the activity of antioxidant enzymes compared to the ZnO NPs-fed rats (P < 0.05). Data also showed the mitigating effects of Vits against ZnO NPs-mediated apoptosis by suppressing the ratio of Bax/Bcl-2 expression and caspase 3,7 activity. This study highlights the protective role of Vits A, C and E against ZnO NPs cardiotoxicity, though at different levels of effectiveness.

Commercial and phytochemical quality in biofortified ‘Orejona’ lettuce with zinc oxide nanoparticles

Crop biofortification is a recent strategy based on the production of plants rich in micronutrients for human consumption. The biofortification of plants with minerals is considered one of the least expensive and most efficient ways to improve the commercial and nutritional quality of horticultural products, in addition to improving crop yields. The objective of the research was to evaluate commercial and phytochemical quality, physiological parameters, and zinc concentration in lettuce leaves (Lactuca sativa L.) after foliar application of zinc oxide nanoparticles (NPsZnO) produced under a hydroponic system. The experiment was carried out with six treatments (0, 5, 10, 15, 20, and 25 mg.L-1 NPsZnO) of five replicates each, under a completely randomized design. Five applications every 15 days of each concentration of NPsZnO were made through the crop cycle. The results show no statistical differences in physiological parameters (height, number of leaves, leaf size, crown perimeter, fresh and dry weight), but that do show a slight tendency to increase on the treated lettuce mainly at concentrations of 20 and 25 mg.L-1. A positive correlation was found between the phytochemical variables (phenolics and total flavonols) and the concentration of NPsZnO. Even though there was not a clear correlation between NPsZnO concentration and the variables of commercial quality; Zn content in the plant tissue was improved, thereby obtaining a biofortified product for the final consumer.

Photocatalytic efficiency of green synthesized ZnO nanoparticles for the degradation of methyl orange dye: A review

The primary global source of water pollution is industrial and textile dyes. These industries produce Highly stable organic dyes that are released untreated into nearby ponds, lakes and rivers. As ZnO nanoparticles (NPs) are inexpensive, non-toxic, chemically and thermally stable, and have a higher UV absorption capacity, they are an effective nano-catalyst for removing hazardous contaminants such as organic and inorganic dyes from wastewater. The green synthesis of synthesised ZnO NPs, their shape and size, and their photocatalytic effectiveness against the dye methyl orange are all described in this paper. The emphasis is on environmentally friendly synthesis of ZnO NPs using plant extracts because the synthesis process impacts the characteristics and applications of ZnO NPs. Additionally, based on recently published literature, the shape and size factors that affect the photocatalytic activity in the degradation of MO dye are highlighted.

An Evaluation of the Biocatalyst for the Synthesis and Application of Zinc Oxide Nanoparticles for Water Remediation—A Review

Global water scarcity is threatening the lives of humans, and it is exacerbated by the contamination of water, which occurs because of increased industrialization and soaring population density. The available conventional physical and chemical water treatment techniques are hazardous to living organisms and are not environmentally friendly, as toxic chemical elements are used during these processes. Nanotechnology has presented a possible way in which to solve these issues by using unique materials with desirable properties. Zinc oxide nanoparticles (ZnO NPs) can be used effectively and efficiently for water treatment, along with other nanotechnologies. Owing to rising concerns regarding the environmental unfriendliness and toxicity of nanomaterials, ZnO NPs have recently been synthesized through biologically available and replenishable sources using a green chemistry or green synthesis protocol. The green-synthesized ZnO NPs are less toxic, more eco-friendly, and more biocompatible than other chemically and physically synthesized materials. In this article, the biogenic synthesis and characterization techniques of ZnO NPs using plants, bacteria, fungi, algae, and biological derivatives are reviewed and discussed. The applications of the biologically prepared ZnO NPs, when used for water treatment, are outlined. Additionally, their mechanisms of action, such as the photocatalytic degradation of dyes, the production of reactive oxygen species (ROS), the generation of compounds such as hydrogen peroxide and superoxide, Zn2+ release to degrade microbes, as well as their adsorbent properties with regard to heavy metals and other contaminants in water bodies, are explained. Furthermore, challenges facing the green synthesis of these nanomaterials are outlined. Future research should focus on how nanomaterials should reach the commercialization stage, and suggestions as to how this ought to be achieved are presented.

Zinc oxide nanoparticles (ZnONPs) influenced seed development, grain quality, and remobilization by affecting the transcription of microRNA 171 (miR171), miR156, NAM, and SUT genes in wheat (Triticum aestivum): a biological advantage and risk assessment study.

Limited studies have been conducted on the role of microRNAs (miRs) and transcription factors in regulating plant cell responses to nanoparticles. This study attempted to address whether the foliar application of zinc oxide nanoparticles (ZnONPs; 0, 10, 25, and 50 mgL-1) can affect miRs, gene expression, and wheat grain quality. The seedlings were sprayed with ZnONPs (0, 10, 25, and 50 mgL-1) or bulk counterpart (BZnO) five times at 72h intervals. The application of ZnONPs at 10 mgL-1 increased the number of spikelets and seed weight, while the nano-supplement at 50 mgL-1 was accompanied by severe restriction on developing spikes and grains. ZnONPs, in a dose-dependent manner, transcriptionally influenced miR156 and miR171. The expression of miR171 showed a similar trend to that of miR156. The ZnONPs at optimum concentration upregulated the NAM transcription factor and sucrose transporter (SUT) at transcriptional levels. However, the transcription of bothNAMandSUTgenes displayed a downward trend in response to the toxic dose of ZnONPs (50 mgL-1). Utilization of ZnONPs increased proline and total soluble phenolic content. Monitoring the accumulation of carbohydrates, including fructan, glucose, fructose, and sucrose, revealed that ZnONPs at 10 mgL-1 modified the source/sink communication and nutrient remobilization. The molecular and physiological data revealed that the expression of miR156 and miR171 is tightly linked to seed grain development, remobilization of carbohydrates, and genes involved in nutrient transportation. This study establishes a novel strategy for obtaining higher yields in crops. This biological risk assessment investigation also displays the potential hazard of applying ZnONPs at the flowering developmental phase.

Improved efficacy of foliar application of zinc oxide nanoparticles on zinc biofortification, primary productivity and secondary metabolite production in dragonhead

Improving medicinal plants' performance in semi-arid regions facing climate change could support sustainable food and agriculture production. Nano-fertilization is considered an emerging strategy for increasing plant production, while avoiding agroecosystem contamination. The objective of this study was to evaluate potential beneficial effects of foliar applications of two sources of zinc (Zn oxide nanoparticles [ZnONPs] as a novel nano-fertilizer and Zn suspension [ZnS] as a conventional fertilizer) on Zn biofortification, primary productivity (biomass) and secondary metabolite production in a medicinally important dragonhead (Dracocephalum moldavica L.) under semi-arid conditions. After 60- and 74-day post-planting, the desired fertilizers were sprayed on to plant foliage. The results showed that leaf Zn bioaccumulation, total chlorophyll, and total flavonoids increased in a dose-dependent manner for both Zn compounds. Sub-toxic levels of Zn-based NPs uptake (up to on average 152.6 mg Zn/kg upon foliar treatment with 160 mg ZnONPs treatment) led to stimulatory effects on total chlorophyll (0.93 g/kg FW), shoot biomass (2168 kg/ha), and essential oil content (3.4 g/kg) and yield (7.4 kg/ha) compared to ZnS treatment or control. The levels of secondary metabolites including neral, geraniol and geranial increased significantly with 160 mg/L ZnONPs compared to equivalent concentration of ZnS or control. The TEM analyses of leaf ultrastructure revealed changes in cellular organelles at 400 mg/L ZnONPs, likely a result of higher oxidative stress (malondialdehyde and H2O2). Higher efficacy of ZnONPs will support greater economic return for dragonhead growers and curtail environmental pollution. Foliar application of 160 mg/L ZnONPs is recommended for optimal micronutrient Zn biofortification, biomass production and essential oil yield in dragonhead, and may serve as a sustainable strategy to promote agricultural yield in (semi-) arid regions.

Biological and Non-Conventional Synthesis of Zinc Oxide Nanoparticles (ZnO-NPs): Their Potential Applications

Zinc oxide nanoparticles (ZnO-NPs) are odorless, white, and insoluble in water and alcohol. There are various applications for ZnO due to its unique semiconducting, piezoelectric, and optical features. Biodegradability and low toxicity are important properties of ZnO nonmaterial. The OH groups on the ZnO surface allow different surface decorating molecules to easily functionalize it. Mineralized in muscle, brain, skin, bone, and other tissues, zinc is a necessary mineral. It is a major component of most enzyme systems and is involved in protein and macromolecule production, neurogenesis, and hematopoiesis, among other functions. Due to its small particle size, Nano-ZnO is easily absorbed by the body. So-called nano-ZnO is commonly utilized in food additives. Chemical surface properties, particle shape, size distribution, and particle reactivity in solution all influence the biological activity of nanoparticles. Determining the morphology, size, and functioning of nanoparticles with regulated structures is critical for biological applications. In this review article, biological and non-conventional synthesis and applications of ZnO-NPs are highlighted in terms of their biological activity. ZnO-NPs have shown therapeutic activity against cancer, diabetes, microbial infection, inflammation, and potential antioxidant activity, which can be used as drug carriers, imaging tools, and biosensors.

A review of the application of zinc oxide nanoparticles in the biological sciences

A review of the application of zinc oxide nanoparticles in the biological sciences

Zinc oxide nanoparticles impact the expression of the genes involved in toxin-antitoxin systems in multidrug-resistant Acinetobacter baumannii.

The aim of this study was to investigate the effect of zinc oxide nanoparticles (ZnO-NPs) on the expression of genes involved in toxin-antitoxin (TA) systems in multidrug-resistant (MDR) Acinetobacter baumannii. Seventy clinical isolates of A. baumannii were collected from variuos clinical samples. Antimicrobial susceptibility test was determined by disk diffusion. Type II TA system-related genes including GNAT, XRE-like, hipA, hipB, hicA, hicB were screened using polymerase chain reaction (PCR). ZnO-NPs prepared and characterized by field emission scanning electron microscopyand X-ray diffraction. MIC of ZnO-NPs of A. baumannii isolates was performed using the microdilution method. The expression of type II TA systems-related genes were assessed with and without exposure to ZnO-NPs using real-time PCR. The highest rate of resistance and sensitivity was observed against cefepime (77.14%), and ampicillin/sulbactam (42.85%), respectively. All A. baumannii isolates were considered as MDR. In this study, three TA loci were identified for A. baumannii including GNAT/XRE-like, HicA/HicB, and HipA/HipB and their prevalence was 100%, 42%, and 27.1%, respectively. There was no significant relationship between the prevalence of these systems and the origin of A. baumannii. Our data showed significant correlations between the presence of HicA/HicB system and resistance to ceftazidime, meropenem, imipenem, and cefepime (p < 0.05), and the presence of HipA/HipB system and resistance to ceftazidime, meropenem, imipenem, and cefepime (p < 0.05). In presence of ZnO-NPs, the expression of all studied genes decreased. GNAT and hicB showed the highest and lowest expression changes by 2.4 folds (p < 0.001) and 1.3 folds (p < 0.05), respectively. This study demonstrates the promising potential of nanoparticles to impact the expression of the genes involved in TA Systems. So, the application of ZnO-NPs may be helpful to design target-based strategies towards MDRs pathogens for empowered clinical applications by microbiologists and nanotechnologists.