Background: Lentil is an important legume crop and plays an important role in human nutrition and soil fertility improvement. It contains 57-60% carbohydrate, 24-26% protein, 3.2% fiberand only 1.3% fat with the full complement of amino acids. It is a potential crop for about 10 million hectares of rice-fallow areas of eastern India. This study aimed to standardized Sulphur and Boron requirements of Lentil in Eastern India. Methods: The experiment was conducted in a glass house during 2018-19 and 2019-20 in a factorial completely randomized design with three replications and 16 treatment combinations of sulphur and boron. The treatment combinations were product of 4 levels each of sulphur and boron as 0, 15, 30 and 45 kg ha-1 (0, 0.067, 0.134 and 0.201 g pot-1) and 0, 1, 2 and 3 kg ha-1 (0, 0.004, 0.009 and 0.013 g pot-1), respectively. Lentil grain yield was observed at harvest and sulphur-containing amino acids, as well as seed nutrient content, were analyzed followingstandard operating procedures. Result: Lentil grain yield was enhanced by 1.85 times with a combined application of 45 kg S and 2 kg B ha-1 with a recommended dose of NPK. The sulphur-containing amino acids namely methionine, cystine and cysteine content showed significant improvement with increasing levels of sulphur application however were not significantly affected with boron levels. The protein content in lentil seed increased considerably with application of sulphur levels as compared to boron levels and it was highest with 30kg S ha-1 + 2kg B ha-1 along with recommended dose of NPK fertilizers.