Application Of SSR Markers Research Articles

"Application of SSR Markers for Testing the Uniformity/Purity Analysis of a Large Wheat (Triticum Aestivum L.) Tilling Population"

"Application of SSR Markers for Testing the Uniformity/Purity Analysis of a Large Wheat (Triticum Aestivum L.) Tilling Population"

Development of SSR Markers Associated with Polima CMS Fertility Restorer in <i>Brassica napus</i> L. and Its Application

The Polima system of cytoplasmic male sterility (Pol CMS) was widely used for hybrid seed production, but with high probability of occurrence of maternal false hybrid due to the fertility of Polima CMS (Pol CMS) line greatly affected by the ambient temperature, therefore, the detection of maternal false hybrids is the key to purity identification. In present study, the SSR markers were designed based on the flanking sequences of Pol CMS restorer gene  Rfp , and application of SSR markers in hybrid seed purity test of rapeseed. The main results were as follows: the sequences of  Rfp  were subjected to basic local alignment search tool queries against the  Brassica rapa  genome to determine chromosome positions, and 32 SSR markers were developed based on the upstream and downstream sequences of  Rfp . Among the 32 SSR markers, one codominant marker was polymorphic between Pol CMS sterile and restorer line, the PCR product length was 162 bp in Pol CMS sterile lines, and 184 bp in restorer lines. Based on the PCR sequence, two insert fragments (15 bp and 7 bp) were identified in Pol CMS restorer lines. The SSR marker co-segregated with plant fertility of individuals in the “fengyou737” F 2  population. The purity of “fengyou737”, “fengyou320” and “fengyou958” were identified by means of PCR-SSR markers and field-planting, and the results of two ways were highly consistent, indicated that the codominant SSR marker tightly linked to  Rfp , and the marker can detect paternal and maternal false hybrids. The present findings will provide technical assistance for hybrid seed purity test and genetic diversity analysis in rapeseed.

Застосування SSR маркерів для оцінювання поліморфізму кукурудзи (Zea mays L.) в експертизі на відмінність, однорідність, стабільність

Застосування SSR маркерів для оцінювання поліморфізму кукурудзи (Zea mays L.) в експертизі на відмінність, однорідність, стабільність

Development and application of SSR markers derived from Bauhinia Strychnifolia a semi-endemic plant in Thailand

ABSTRACTBauhinia strychnifolia is a rare and semi-endemic plant in Thailand. Microsatellite-enriched libraries were constructed using (CT)15/(CAG)15 and (GT)15/(AAG)15 probes. Fourteen polymorphic loci produced a total of 104 alleles with a mean of 7.4 alleles per locus. The average expected and observed heterozygosity were 0.727 and 0.497, respectively. The polymorphism information content value ranged from 0.335 to 0.910. Nine microsatellite loci were successfully transferred across species to B. galpiniii, B. Siamensis, and B. tomentosa. The information obtained in this study will be useful in establishing a conservation strategy for B. strychnifolia in Thailand and for genetic studies of other Bauhinia sp.

Application of SSR Markers in Determination of Putative Resistance to SPVD and Genetic Diversity among Orange Fleshed Sweet Potato

Application of SSR Markers in Determination of Putative Resistance to SPVD and Genetic Diversity among Orange Fleshed Sweet Potato

IDENTIFICATION OF SIMPLE SEQUENCE REPEAT (SSR) MARKERS LINKED TO FLOWERING TIME IN ALMOND BY BULKED SEGREGANT ANALYSIS (BSA)

In this study, flowering dates were evaluated during two years in a F1 almond progeny of seventy eight seedlings from the cross between the intermediate flowering Italian cultivar ‘Tuono’ and the extra-late flowering Iranian cultivar ‘Shahrood-12’. In addition, a modified-bulk segregant analysis in combination with the application of SSR markers was used to identify molecular markers linked to flowering time. Seventy five nuclear SSR markers spanning the whole almond genome were assayed in the molecular characterization of bulks (extra-early, early, late and extra-late) consisting in several descendants selected from the almond progeny studied. Results showed a quantitative inheritance of flowering date in the almond progeny studied. The seedlings evaluated showed a wide range of flowering dates between both progenitors although some of these descendants were earlier than the early progenitor ‘Tuono’. Two microsatellite loci (CPPCT008 and EPDCU2584) were found to be tightly linked to this important agronomic trait. Finally, we discuss the development of efficient marker-assisted selection strategies applied to almond and other Prunus breeding programs.

Marker assisted introgression of opaque2 gene into herbicide resistant elite maize inbred lines

Marker assisted selection in combination with conventional breeding can greatly accelerate the introgression of modified opaque2 genotype into herbicide resistant maize. By combining these two approaches, time and costs are greatly minimized. The application of opaque2 allele specific SSR markers was done on materials already undergoing selection in a breeding program for converting herbicide resistant maize lines into quality protein maize (QPM) which is the equivalent of modified opaque2 phenotype. The breeder had selected QPM lines using the light table in the previous cycle and we used leaf samples to extract DNA for analysis of the presence of the opaque2 gene using SSR markers. Two co-dominant SSR markers phi057 and umc1066 and a dominant marker phi112 were used. Umc1216, a modifier marker was also tested in combination with the opaque2 markers with the objective of using the marker to select for modifiers for the opaque2 phenotype. The modified FTA paper technology protocol was applied in field sampling. The results showed 97% of the lines were opaque2 while 3% were non-opaque2. Both methods of conventional breeding using light table and marker assisted selection (MAS) were comparable. However, the application of SSR markers and the FTA technology offers the breeder a fast, time saving, reliable and less labour intensive method of screening QPM maize during the early growing stages instead of having to wait to screen the kernels on the light table after harvesting. Moreover, the routine biochemical analysis for high lysine and tryptophan levels need not be carried out at each backcross since the presence of the opaque2 gene is confirmed with markers.