Abstract
In this study, flowering dates were evaluated during two years in a F1 almond progeny of seventy eight seedlings from the cross between the intermediate flowering Italian cultivar ‘Tuono’ and the extra-late flowering Iranian cultivar ‘Shahrood-12’. In addition, a modified-bulk segregant analysis in combination with the application of SSR markers was used to identify molecular markers linked to flowering time. Seventy five nuclear SSR markers spanning the whole almond genome were assayed in the molecular characterization of bulks (extra-early, early, late and extra-late) consisting in several descendants selected from the almond progeny studied. Results showed a quantitative inheritance of flowering date in the almond progeny studied. The seedlings evaluated showed a wide range of flowering dates between both progenitors although some of these descendants were earlier than the early progenitor ‘Tuono’. Two microsatellite loci (CPPCT008 and EPDCU2584) were found to be tightly linked to this important agronomic trait. Finally, we discuss the development of efficient marker-assisted selection strategies applied to almond and other Prunus breeding programs.
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