Application Of Low Power Laser Research Articles

THULIUM:YAG LASER ENUCLEATION OF THE PROSTATE (ThuLEP) - OUR EXPERIENCE IN 246 PATIENTS.

New minimally invasive procedures to treat benign prostatic hyperplasia (BPH) have been developed in the last 20 years. With the introduction of laser enucleation techniques in prostate surgery, previous standard surgical procedures (open retropubic or transvesical adenectomy and transurethral resection of the prostate) have become less relevant, especially in case of very large prostates. The objective of this paper is to describe the experience and results of thulium:YAG laser application in BPH treatment in our department. In the last five years, 246 patients underwent thulium:YAG laser enucleation of the prostate (ThuLEP) due to BPH or lower urinary tract symptoms following complete urologic evaluation. The mean age of our patients was 73 (range 51-95) years and mean duration of surgery was 92 minutes. The mean weight of enucleated tissue was 73 grams. A low rate of intraoperative and early postoperative complications and short length of stay proved minimal invasiveness of this procedure, which results in significant improvements in urodynamic parameters and substantially reduces the post-void residual urine volume. The most important surgical and technical characteristics (enucleation and low-power laser application) make ThuLEP a safe and efficient surgical method not limited by the prostate volume. ThuLEP has a potential to displace the current standard surgical procedures to treat BPH.

Photodynamic Therapy, Photobiomodulation and Acetonide Triamcinolone 0.1% in the Treatment of Oral Lichen Planus: A Randomized Clinical Trial.

Objective: To evaluate the efficacy of photodynamic therapy (PDT) and photobiomodulation (PBM) in the treatment of oral lichen planus (OLP) in comparison with the use of topical corticosteroids. Material and methods: Sixty patients with OLP were randomized to three groups: group 1 photodynamic therapy applied once a week for four sessions, with orabase cream; group 2 low-power laser application with orabase cream; and group 3 inactive laser with triamcinolone acetonide 0.1%. Patient pain was evaluated, and the Thongprasom severity score, the Oral Health Impact Profile-14 (OHIP-14), and the Hamilton anxiety and depression scale at one and three months of follow-up. (ClinicalTrials.gov Identifier: NCT05127083). Results: Pain decreased significantly over time in all groups, though the symptoms relapsed over follow-up at one and three months in group 3. The OHIP-14 score improved significantly in groups 1 and 2 (p < 0.05), and this improvement was maintained after three months. Lesion resolution evaluated by the Thongprasom score at one month showed significant differences between groups 1 and 3 (p = 0.032) and between groups 2 and 3 (p = 0.024). Conclusions: Photodynamic therapy and photobiomodulation once a week for four weeks are safe and non-invasive treatment options, with the important advantage of lacking adverse effects. Further studies are needed to confirm it.

Photodynamic Therapy in the Healing of the Areolo-Papillary Complex after Mastectomy—Case Report

Some breast reconstruction surgeries after mastectomy, or even reductive mammoplasty, may evolve unexpectedly when the areolopapillary complex heals and develop dehiscences, necrosis and delayed healing. The purpose of this case report was to verify the efficacy of photodynamic therapy in wound healing of the areolopapillary complex. This was a case report of a female patient with diabetes, fibromyalgia and diagnosed with breast cancer, with necrotic areolopapillary complex, after debridement, photodynamic therapy started with clobetasol propionate associated with methylene blue and low power laser application. At the beginning of the treatment with photodynamic therapy, the wound had 6 centimeters of diameter, and after 19 sessions, the wound was closed completely with normalization of the color of the nipple. The photodynamic therapy is advancing a lot in healing of infected wounds and complex wounds. It can be said that in this case report, photodynamic therapy was fundamental for the complete healing, being considered a promising treatment and another alternative for dermatofunctional physiotherapists.

Estudo comparativo do uso de extrato de Pfaffia glomerata e do laser de baixa potência (Hélio-Neônio) na cicatrização de feridas em ratos

RACIONAL: A Pfaffia glomerata é planta utilizada na medicina popular como tônico, antidiabético, para melhorar o processo de cicatrização, neutralizar distúrbios gástricos e antirreumático. OBJETIVOS: Comparar a contração da ferida e a neovascularização com o uso da Pfaffia glomerata e do laser de baixa potência em dorso de ratos. MÉTODOS: Foram utilizados 40 ratos da linhagem Wistar nos quais realizaram-se feridas cirúrgicas com punch de 3 mm de diâmetro na parte superior direita do dorso onde nenhuma substância foi aplicada e nesses mesmos animais foram também realizadas feridas cirúrgicas no lado esquerdo na região inferior, onde foi aplicado o extrato das raízes de Pfaffia glomerata (Spreng.) Pedersen, Amaranthaceae, obtendo-se desta maneira os grupos controle e planta nos mesmos animais. Em outros 40 animais, foi utilizado o laser de baixa potência. Nas datas programadas em 48 horas, uma, duas e três semanas foram tomadas as medidas da contração da ferida. Microscopicamente os resultados foram analisados utilizando-se imunoistoquímica como fator VIII para observar a densidade vascular. RESULTADOS: Macroscopicamente não houve diferença estatisticamente significante com relação à contração da ferida entre os grupos planta e laser, obtendo ambos resultados superiores ao grupo controle. Dentro desta variável, o resultado com diferença estatisticamente significante ocorreu dentro do grupo laser na comparação dos subgrupos: 48 horas vs. uma semana (p=0,008). A análise do Fator VIII mostrou significância estatística no subgrupo de uma semana da planta em relação ao laser(p=0,09). CONCLUSÕES: Não houve diferença estatisticamente significante com relação à contração da ferida entre os grupos planta e laser, obtendo ambos resultados superiores ao grupo controle no final do estudo; contudo, microscopicamente, o grupo planta obteve valor superior ao grupo laser quanto à neovascularização em uma semana, mas após esse período, houve equilíbrio com os outros grupos.

Morphometric and histological analysis of low-power laser influence on bone morphogenetic protein in bone defects repair

Bone morphogenetic proteins (BMPs) are secreted signaling molecules belonging to the transforming growth factor-beta (TGF-beta) superfamily. The objective of this study was to determine how gallium-aluminum-arsenium (GaAlAs) 650 nm laser influenced the action of BMPs on bone defects created in rat femurs. The sample consisted of 24 male albino Wistar rats. Group 1 was composed of rats with bone defects filled with bone-inducing substance, with the application of low-power laser. Group 2 contained rats with bone defects filled with a bone-inducing substance, without the application of low-power laser. Group 3 rats had bone defects not filled with a bone-inducing substance, with the application of low-power laser. Group 4 rats had bone defects and no treatment (control group). A bone defect was produced with drills. In groups 1 and 2 the defects were filled with a bone-inducing substance. The animals were treated with GaAlAs (50 mW) laser, energy density 4 J/cm(2), for 80 ss on a 1 cm(2) area. Groups 2 and 4 were used as control. Bone samples were removed for histological procedures and morphometric analysis on the 7th, 14th and 21st days after surgery. Results obtained were subjected to statistical analysis. Rejection level for the null hypothesis was 0.05. Statistical differences were found in the comparison between group 1 (G1), G2, G3 and G4 [analysis of variance (ANOVA); P < 0.0134]. There was a statistically significant correlation between groups 1 and 4 (P < 0.01). The results of other correlations by Tukey's post-hoc test were: group 1 vs group 3 (P = 0.341), group 1 vs group 2 (P = 0.862), group 2 vs group 4 (P = 0.061), group 2 vs group 3 (P = 0.744), and group 3 vs group 4 (P = 0.249). We concluded that the association of low-power laser with a bone-inducing substance produced better results than when low-power laser or BMPs were used alone.

Effect of 650 nm low-power laser on bone morphogenetic protein in bone defects induced in rat femors&lt;A NAME="volta1"&gt;&lt;/A&gt;

To investigate the influence of 650 nm GaAlAs laser on the action of bone morphogenetic protein (BMP) in bone defects produced in rat femurs. The sample consisted of 12 male albino Wistar rats (Rattus norvegicus). The animals were randomly divided into four experimental groups. After undergoing anesthesia, the fur was removed from the lateral face of the right thigh and surgical dissection was performed to view the femur region. A bone defect was created using a spherical diamond-tipped drill bit. In groups 1 and 2, the defect was filled with a paste of Gen-Tech bone-inducing substance. The animals were treated with GaAlAs laser, at a predetermined dose of joules/cm(2) for 80 seconds, over an area of 1 cm(2). Groups 2 and 4 were used as controls. Bone samples were removed to perform histological procedures and morphometric analyses on the 7th, 14th and 21st days after the operation. The results obtained were subjected to statistical analysis using ANOVA variance according to two criteria, with four repetitions, followed by the post hoc t test. The rejection level for the nullity hypothesis was 0.05 or 5% (alpha < or = 0.05). In comparisons between G1, G2, G3 and G4, p = 0.024 was observed. In statistical comparisons using the t test for paired samples, only G1 vs. G4 presented a statistically significant result (p = 0.021). The association of low-power laser application and Gen-Tech bone-inducing substance achieved a better result than laser application alone or BMP use alone.

The current status of laser applications in dentistry.

A range of lasers is now available for use in dentistry. This paper summarizes key current and emerging applications for lasers in clinical practice. A major diagnostic application of low power lasers is the detection of caries, using fluorescence elicited from hydroxyapatite or from bacterial by-products. Laser fluorescence is an effective method for detecting and quantifying incipient occlusal and cervical carious lesions, and with further refinement could be used in the same manner for proximal lesions. Photoactivated dye techniques have been developed which use low power lasers to elicit a photochemical reaction. Photoactivated dye techniques can be used to disinfect root canals, periodontal pockets, cavity preparations and sites of peri-implantitis. Using similar principles, more powerful lasers can be used for photodynamic therapy in the treatment of malignancies of the oral mucosa. Laser-driven photochemical reactions can also be used for tooth whitening. In combination with fluoride, laser irradiation can improve the resistance of tooth structure to demineralization, and this application is of particular benefit for susceptible sites in high caries risk patients. Laser technology for caries removal, cavity preparation and soft tissue surgery is at a high state of refinement, having had several decades of development up to the present time. Used in conjunction with or as a replacement for traditional methods, it is expected that specific laser technologies will become an essential component of contemporary dental practice over the next decade.

低出力レーザーの星状神経節近傍 (SG) 照射治療

アトピー性皮膚炎を通常の対症療法に加え睡眠, 糖分の減量, アルコール等のライフスタイルおよび低出力レーザー療法にて治療した. GaAlAs 半導体を使った出力1000mW, 波長830nmのレーザー光を出す半導体レーザー照射装置 (Mochida LUKETRON Medilaser soft Matsusita Osaka) を使用した. 照射方法は頚部の星状神経節近傍に片側1分で両側に計2分間照射 (SG-LLLT) した. 照射は原則として1日1回とし, 週1～2回の頻度で実施した. 症状の観察項目は?痒, 紅斑, 乾燥および総合的に見た症状である総合的症状の4項目とし, 評価方法は初診時を10, 症状のない状態あるいは理想的な状態を0とし, その時の症状を患者自身が判断し数値化した, その結果, スコアが5点以上低下した改善症例は?痒が100例中75例 (75%), 紅斑が96例中71例(74%), 乾燥が89例中47例 (52.8%) および総合的症状が101例中72例 (71.3%) であった. 睡眠と各々の皮膚症状との関係は SG-LLLT 後5≧に改善した症例数で X2 乗検定にて検討した. その結果, 睡眠の改善が5≧の症例数はそれぞれの症状が5≧の症例数と比較すると, ?痒 (p<0.0005), 紅斑 (p<0.00001), 乾燥 (p<0.001) および総合的症状 (p<0.000001) できわめて有意な一致をみた. SG-LLLT の照射日数 (n) と睡眠との関係は睡眠の改善が5≧の症例数は1<, 2<, 3<, 4< および5< の症例数に比べそれぞれ1≧(p<0..05), 2≧(p<0.05), 3≧(p<0.01), 4≧(p<0.01) および5≧(p<0.05) で有意に少なく, 睡眠の改善には SG-LLLT が5日以上が望ましいと考えた.

低出力レーザーの整形外科への応用

低出力レーザーの整形外科における応用は各種疾患の疼痛のコントロール, 慢性関節リウマチを中心とした関節炎のコントロールなどに行われている.レーザーのRAの関節炎のコントロールのメカニズムの解明のために実験モデルであるラットのアジュバント関節炎を作製し, Nd-YAGレーザーを照射した. Nd-YAGレーザー照射によりアジュバント関節炎はコントロールされ, 関節炎のH・E染色およびIa抗原,T4,T8、 抗原による免疫組織学的検討でも効果は実証された. RAの手術時採取された滑膜もNd-YAGレーザー照射によりIL-Iを中心としたサイトカイン放出の抑制が起こった. RAの関節炎にNd-YAGレーザーを照射すると皮下の血流量の著明な増加を認め, 症状も改善した,各種の疼痛性疾患に対する半導体レーザー照射のタブルブラインド方式による評価では, レーザー照射は非照射に比べ, 疼痛性疾患をよりよくコントロールしていた.

Welcome to Munich

Summary Ladies and Gentlemen, dear colleagues, On behalf of the Board of the German Society for Laser Medicine a very warm welcome to our 13th Annual Meeting. Like the previous meetings also the present one wants to give a comprehensive overview on current Medical Laser Application in Germany, Austria and Switzerland. We are happy about the great number of qualified scientific contributions from the medical specialties, basic research as well as industry. To offer a platform for interdisciplinary crosstalk we have chosen sessions with specified subjects, e.g. interstitial thermotherapy, fluorescence diagnostics, photodynamic therapy, low power laser application. We are delighted to have won as chairmen highly reputated representatives of all major research areas. We trust that not only this but also the great opportunities here at the Messe Munchen and the Laser 2001 will offer us an exciting ambience and a fruitful dialogue. Saying this, my warmest thanks go to all staff members and also the Messe Munchen as well our industrial partners for the huge amount of work they have already accomplished so far. Let's fill this meeting with life and spirit by our talks and our ideas. Let's create a great future for Laser Medical Application. We are happy to welcome you all to Munich!

The little laser that could: applications of low power lasers in clinical flow cytometry.

The little laser that could: applications of low power lasers in clinical flow cytometry.

Practical Applications of Low Power Lasers

"Practical Applications of Low Power Lasers." Optica Acta: International Journal of Optics, 24(12), p. 1255