IntroductionOld-growth forests (OGFs) are vital for global biodiversity, acting as irreplaceable carbon sinks, fostering ecosystem stability, and preserving unique ecological and cultural heritage. There are three old-growth forests in the Republic of Srpska/Bosnia and Herzegovina (Lom, Janj, and Perućica). They are dominated by mixed forests of beech, fir, and spruce (Piceo-Abieti-Fagetim illyricum). The satellite imagery results confirm that the vegetational indices are crucial for monitoring old-growth forests. Vegetation indices provide significant information for monitoring forest ecosystems. Based on Copernicus Sentinel-2 images, remote sensing is enabled, providing data in various spatial and spectral resolutions. The analysis of images related to old-growth forests allows for monitoring the spatial dynamics of changes in vitality and the health status of forests. This research aims to characterize old-growth forests from space through vegetation indices to obtain a more in-depth analysis of the ecological condition of these forests.MethodsThe research methodology includes the application of Sentinel 2A satellite imagery, which is taken from the European Space Agency (ESA) database. The Sentinel 2 mission consists of two identical satellites (Sentinel 2A and Sentinel 2B) that have multispectral sensors with 13 spectral channels (bands) at spatial resolutions of 10, 20, and 60 m. The analysis included the normalized difference vegetation index (NDVI), enhanced vegetation index (EVI), soil-adjusted vegetation index (SAVI), and visible atmospheric resistance index (VARI). For determining the significance of differences in vegetation index magnitudes between old-growth forests, a one-way analysis of variance with equal repetitions was employed.Results and discussionThe parts of old-growth forests are located in different stages of development, and the trees in them are characterized by different degrees of vitality and have different values of vegetation indices. It is assumed that parts of old-growth forests in the terminal phase of development with a large number of diseased and rotting trees have the smallest sizes of vegetation indices. This research aims to characterize old-growth forests from space through vegetation indices to obtain a more in-depth analysis of the ecological condition of these forests. Using satellite imagery and their analysis of the obtained sizes of vegetation indices, it is observed that there is a significant difference in their sizes between old-growth forests. The Janj old-growth forest stands out as the area with the highest NDVI values, followed by the Lom old-growth forest, while the Perućica old-growth forest has the lowest NDVI values. However, the Perućica old-growth forest has significantly higher values of EVI and SAVI vegetation indices, while the other two old-growth forests have approximately similar values. The VARI index values are highest in the Lom old-growth forest, slightly lower in the Perućica old-growth forest, and lowest in the Janj old-growth forest. The results of these studies indicate statistically significant differences in vegetation index magnitudes between old-growth forests. The application of Copernicus satellite data enables the monitoring of changes in vegetation index values, which is of great assistance in spatially defining the developmental stages in old-growth forests (OGFs). Considering the relatively small number of sample areas covered by these studies, similar research needs to be conducted on a larger number of sample areas (replications), especially during the vegetation period.