Greenhouse horticulture is one of the most intensive agricultural systems, with the advantages of environmental parameter control (temperature, light, etc.), higher efficiency of resource utilization (water, fertilizers, etc.) and the use of advanced technologies (hydroponics, automation, etc.) for higher productivity, earliness, stability of production and better quality. On the other hand, climate change and the application of high inputs without suitable management could have negative impacts on the expansion of the greenhouse horticulture sector. This special issue gathers twelve papers: three reviews and nine of original research. There is one review that focuses on irrigation of greenhouse crops, while a second surveys the effects of biochar on container substrate properties and plant growth. A third review examines the impact of light quality on plant–microbe interactions, especially non-phototrophic organisms. The research papers report both the use of new technologies as well as advanced cultivation practices. In particular, new technologies are presented such as dye-sensitized solar cells for the glass cover of a greenhouse, automation for water and nitrogen deficit stress detection in soilless tomato crops based on spectral indices, light-emitting diode (LED) lighting and gibberellic acid supplementation on potted ornamentals, the integration of brewery wastewater treatment through anaerobic digestion with substrate-based soilless agriculture, and application of diatomaceous earth as a silica supplement on potted ornamentals. Research studies about cultivation practices are presented comparing different systems (organic-conventional, aeroponic-nutrient film technique (NFT)-substrate culture), quantitative criteria for determining the quality of grafted seedlings, and of wild species as alternative crops for cultivation.