Nitrogen leaching loss in paddy fields is one of the main ways of farmland non-point source pollution. To explore the suitable fertilization of rice fields in the Erhai Lake Basin and reduce the nitrogen loss from paddy fields, a field experiment was conducted by setting single applications of chemical or organic fertilizer, combined organic and inorganic application, and single application of controlled release fertilizer under reduced nitrogen conditions. The results showed that, compared with the conventional fertilization treatment(CF), there was no significant difference in rice grain and straw yield between the single chemical fertilizer treatment(T1) and the organic-inorganic combined treatment(T3); the single organic fertilizer treatment(T2) decreased the rice grain yield by 13.0%, and decreased straw yield by 17.1%; single application of controlled-release fertilizer(T4) increased rice grain and straw yield by 15.7% and 21.0%, respectively. Further, compared with CF, the single application of chemical fertilizer(T1), organic fertilizer(T2), and organic-inorganic combined application(T3) reduced the total nitrogen leaching loss at 30 cm depths by 26.9%, 18.0%, and 33.9%, respectively. The loss of ammonia nitrogen leaching with T1, T2, and T3 decreased by 24.4%, 36.9%, and 36.6%, respectively, and the loss of nitrate nitrogen leaching decreased by 40.2%, 4.8% and 46.4%. The total nitrogen leaching at 60 cm soil depths was reduced by 34.2%, 26.3%, and 42.1%, the loss of ammonia nitrogen leaching was reduced by 31.4%, 35.7%, and 46.6%, and the loss of nitrate nitrogen leaching was reduced by 8.0%, 10.1%, and 23.9% for T1, T2, and T3, respectively. The total nitrogen loss at 30 and 60 cm depths increased by 41.6% and 14.0% in the single application of controlled release fertilizer(T4) treatment. Considering factors such as agronomic and environmental benefits of different fertilization modes, T1 and T3 are suitable environmentally friendly alternative fertilization modes.