Application Of Chitosan Research Articles

Preparation, characterization, and application of chitosan–kaolin-based nanocomposite in magnetic solid-phase extraction of tetracycline in aqueous samples

Preparation, characterization, and application of chitosan–kaolin-based nanocomposite in magnetic solid-phase extraction of tetracycline in aqueous samples

Chitosan-Based Nanomaterial as Immune Adjuvant and Delivery Carrier for Vaccines.

With the support of modern biotechnology, vaccine technology continues to iterate. The safety and efficacy of vaccines are some of the most important areas of development in the field. As a natural substance, chitosan is widely used in numerous fields-such as immune stimulation, drug delivery, wound healing, and antibacterial procedures-due to its good biocompatibility, low toxicity, biodegradability, and adhesion. Chitosan-based nanoparticles (NPs) have attracted extensive attention with respect to vaccine adjuvants and delivery systems due to their excellent properties, which can effectively enhance immune responses. Here, we list the classifications and mechanisms of action of vaccine adjuvants. At the same time, the preparation methods of chitosan, its NPs, and their mechanism of action in the delivery system are introduced. The extensive applications of chitosan and its NPs in protein vaccines and nucleic acid vaccines are also introduced. This paper reviewed the latest research progress of chitosan-based NPs in vaccine adjuvant and drug delivery systems.

Antibacterial Mechanism of Chitosan-Gentamicin and Its Effect on the Intestinal Flora of Litopenaeus vannamei Infected with Vibrio parahaemolyticus.

To explore the application of chitosan-gentamicin conjugate (CS-GT) in inhibiting Vibrio parahaemolyticus (V. parahaemolyticus), which is an important pathogen in aquatic animals worldwide, the antimicrobial activity of CS-GT and the effects of a CS-GT dose on the intestine histopathology and intestinal flora of V. parahaemolyticus-infected shrimps were explored. The results showed that CS-GT possessed broad-spectrum antibacterial activity, with minimum inhibitory concentration (MIC), minimum bactericidal concentration (MBC) and half inhibitory concentration (IC50) of 20.00 ± 0.01, 75.00 ± 0.02 and 18.72 ± 3.17 μg/mL for V. parahaemolyticus, respectively. Further scanning electron microscope and cell membrane damage analyses displayed that the electrostatic interaction of CS-GT with cell membrane strengthened after CS grafted GT, resulting in leakage of nucleic acid and electrolytes of V. parahaemolyticus. On the other hand, histopathology investigation indicated that high (100 mg/kg) and medium (50 mg/kg) doses of CS-GT could alleviate the injury of a shrimp's intestine caused by V. parahaemolyticus. Further 16S rRNA gene sequencing analysis found high and medium dose of CS-GT could effectively inhabit V. parahaemolyticus invasion and reduce intestinal dysfunction. In conclusion, CS-GT possesses good antibacterial activity and could protect shrimps from pathogenic bacteria infection.

Development and Application of Dual-Sensors Label in Combination with Active Chitosan-Based Coating Incorporating Yarrow Essential Oil for Freshness Monitoring and Shelf-Life Extension of Chicken Fillet.

This study aimed for the application of active chitosan coating incorporating yarrow essential oil (YEO) together with the development of an on-package sensor label based on bromocresol purple (BCP) and methyl red (MR) for shelf-life extension and freshness monitoring of chicken breast fillet. Physiochemical and microbiological attributes of chicken meat coated with sole chitosan, YEO, and chitosan + YEO were compared with those of uncoated (control) samples. Chitosan + YEO coated chicken meat stayed fresh with no significant changes (p > 0.05) in pH (5.42-5.56), TVB-N (12.55-15.36 mg N/100 g), TBARs (0.35-0.40 mg MDA/kg) and total aerobic psycrotrophic bacteria (3.97-4.65 log CFU/g) in days 1-15. There was no response of the dual-sensors label toward the variation in chemical and microbiological indicators of chicken meat coated with chitosan + YEO. However, either uncoated, sole chitosan, or sole YEO treatments indicated a three-stage freshness status with the fresh stage belonged to a period earlier than day 7 (with no distinct color change in both sensor labels); the semi-fresh stage corresponded to storage days between 7-9, wherein a gradual color change appeared (MR from pink to orange, BCP from yellow to light purple); and the spoiled stage occurred in day 9 onward with a drastic color change (MR from orange to light yellow, BCP from light purple to deep purple). In general, the dual-sensors successfully responded to the variation of chemical and microbiological indicators and visual color of uncoated samples during storage time. Based on the obtained results, the application of chitosan + YEO coating efficiently prolonged the freshness of chicken breast meat, where on-package dual-sensors systems were able to detect the freshness stages of meat samples during storage time.

The Application of Chitosan for Protection of Cultural Heritage Objects of the 15-16th Centuries in the State Tretyakov Gallery.

Microorganisms are one of the main factors in the deterioration of cultural heritage, in particular art paintings. The antiseptics currently used in painting have significant limitations due to insufficient effectiveness or increased toxicity and interaction with art materials. In this regard, the actual challenge is the search for novel materials that effectively work against microorganisms in the composition with painting materials and do not change their properties. Chitosan has pronounced antimicrobial properties but was not used previously as an antiseptic for paintings. In our study we developed a number of mock layers based on sturgeon glue, supplemented which chitosan (molecular weight 25 kDa or 45 kDa), standard antiseptics for paintings (positive controls) or without additives (negative control). According to Fourier transform infrared spectroscopy and atomic force microscopy, the addition of chitosan did not significantly affect the optical and surface properties of this material. The ability of chitosan to effectively protect paintings was shown after inoculation on the created mock-up layers of 10 fungi-destructors of tempera painting, previously isolated from cultural heritage of the of the 15-16th centuries in the State Tretyakov Gallery, on the created mock layers. Our study demonstrated the principled opportunity of using chitosan in the composition of painting materials to prevent biodeterioration for the first time.

Essential oil composition, physiological and morphological variation in Salvia abrotanoides and S. yangii under drought stress and chitosan treatments

Several Salvia species are among the most valuable aromatic herbs used for industrial and pharmaceutical applications. Hence, under greenhouse trial conditions, the effects of chitosan treatment (0, 100, and 200 mg L−1) and water deficiency stress on the morphological, physiological, and biochemical features of two Salvia species were examined. Significant changes were seen in chlorophyll a, root volume, dry and fresh weight, and H2O2 concentration as a result of drought stress. Significant influence of chitosan was found for all studied parameters except root length and malondialdehyde (MDA) content. Hydrogen peroxide (H2O2), proline, and MDA were elevated, while photosynthetic pigments decreased under drought stress. The highest essential oil (EO) content (2.20% d.b.) was recorded under moderate stress condition in the absence of chitosan treatment. Using chitosan topically, it is possible to offset the impact of water scarcity on EO content decline and enhance EO compositions. The compensatory effects of chitosan application under stress conditions were observed on the abundance of EO constituents, such as 1,8-cineol, camphor, bornyl acetate, α-bisabolol, α-cadinol, and α-humulene. Moreover, present results suggested that chitosan application can alleviate the drought damage in studied Salvia species.

Chitosan–caffeine formulation as an ecological preservative in wood protection

Nowadays, taking into account environmental degradation, climate changes, as well as, environmentally friendly regulations and increasing ecological awareness of consumers, methods of wood protection without negative impact on the environment are being intensively developed. In this study, a mixture of chitosan and caffeine was proposed as ecological wood preservative. The aim of the research was to evaluate the application of chitosan (medium and high molecular weight) as an agent limiting the leaching of caffeine from the structure of treated wood. The resistance of pine wood treated with chitosan–caffeine preparations and ingredients of these preparations (caffeine and chitosan) to brown rot fungus—Coniophora puteana was assessed. The degree of caffeine leaching from the treated wood was determined by chromatographic analysis (caffeine concentration) and elemental analysis (nitrogen concentration). The results showed that chitosan limited caffeine leaching from wood, which was confirmed by chromatographic analysis and changes in the wood structure observed in FTIR spectra. Moreover, wood treated with chitosan–caffeine preparations showed resistance against C. puteana (mass loss about 0.5%); however, antifungal activity was reduced when treated wood was subjected to leaching with water (mass loss about 10%). The obtained results suggest that the chitosan–caffeine preparations can be promising ecological preservatives for the wood treatment.

Chitosan on the surface of nanoparticles for enhanced drug delivery: A comprehensive review.

The ability of chitosan (CTS) to promote mucoadhesion, trigger positive/negative surface interactions, and open tight junctions has inspired researchers to coat lipid-based and polymeric-based nanoparticles with CTS in an attempt to reach new heights in the delivery of drugs and nutraceuticals across different routes. This article discusses literature relevant to the pharmaceutical and biomedical area published in the last 10years on nanoparticles overlaid with CTS. Herein, we addressed the technical aspects of the coating procedure by adding CTS solution to nanoparticles that have already been produced or during the production phase. Besides, we reviewed the applications of CTS coated nanoparticles as drug delivery systems in the oral and non-oral routes of administrations. Special attention was paid to the physicochemical and biological benefits of the CTS coating, such as improving physicochemical stability, enhancing cell and tissue interactions, controlled drug release, and augmentation of active substance bioavailability and efficacy. Moreover, this review projects the current standing and future prospects of the delivery system. The future calls for more investigations on therapeutic proteins, genes and vaccines as potential cargos. Extensive studies on the merits of integrating CTS with hydropolymer-synthesized nanoparticles and using longer-chain and chemically-modified variants of CTS are also warranted.

Alleviation of Salt Stress with Chitosan Foliar Application and Its Effects on Growth and Development in Tomato (Solanum lycopersicum L.)

Environmental and climatic fluctuations as well as abiotic stress factors affect agricultural production and cause a loss in quality and yield. It is important to find alternative solutions for the sustainability of agricultural production to feed the increasing population. Salt stress is one of the most devastating abiotic stress factors and tomato (Solanum lycopersicum L.) production is also affected by salt stress since it needs extensive irrigation for high yield. The exogenous application of some plant inducers showed promising results in the induction and improvement of plant tolerance to stress factors. Chitosan (2-amino-2-deoxy-b-D-glucosamine), one of the organic compounds, is getting significant attention in agriculture with its potential. Here, we evaluated the potential of chitosan application for salt stress tolerance on tomato. 0.03% and 0.05% chitosan solutions were applied as a foliar spray to the plant and salt tolerance improvement were investigated under untreated (0 mM NaCl) and 100 mM NaCl conditions. The growth-related (root and shoot diameters, above and below-ground biomass, number of leaves and branches, and plant height), photosynthetic parameters (chlorophyll a, b, total carotenoid content), and ion leakage were investigated. According to the results, chitosan application improves plant development in both untreated and salt-stress conditions and improved plant growth. Also, photosynthetic parameters showed that the application of chitosan increased chlorophyll contents under untreated conditions. Our result suggests that the application of chitosan may have a promising effect on salt stress tolerance and further research may shed light on its molecular mechanisms.

Synthesis, Characterization and Catalytic Applications of Chitosan Based Schiff Base Complexes of Copper (II)

Abstract: Chitosan Schiff Base was synthesized using conventional method and its corresponding metal complex was synthesized using Copper sulphate crystals. CSB and the metal complex were characterized using analytical and spectroscopic techniques like FT-IR and SEM. The excellent catalytic activity of the synthesized CSB-Cu complex was studied with Knoevenagel Reaction

Potential application of chitosan stabilized silver nanoparticles for simultaneous control of dengue virus and mosquito vectors

Dengue fever pandemic caused by dengue virus has been claimed for many lives, however, no specific treatment is available. Prevention based on vector control posed to be the most effective measure so far. The application of chitosan stabilized silver nanoparticle (AgNPs) to control larvae and adult mosquitoes has recently discovered, but their ability to inhibit Dengue virus has scarcely discussed. In this study, chitosan stabilized AgNPs have been prepared and tested against dengue virus type 1. The results showed that chitosan stabilized AgNPs can inhibit 96.66% and 98.33% dengue virus type 1 at 12.50 ppm and 25.00 ppm, respectively, without any toxicity to BHKFcɣ cells. This study confirms that chitosan stabilized AgNPs have a highly antiviral ability against dengue virus in addition to its mosquitocidal properties. This allows one to control both virus and vector simultaneously. The study suggested that chitosan stabilized AgNPs could be an effective tool to prevent the dengue epidemic.

Effects of the Foliar Application of Water-soluble Chitosan or Na2SiO3 Fertilizer on the Pb Accumulation by a Low-Pb Accumulator Brassica napus Grown on Farmland Surrounding a Working Smelter.

Field experiments were conducted to investigate the effects of two foliar fertilizers, water-soluble chitosan (WSC) and Na2SiO3 (Si), on the accumulation of Pb by a low-Pb accumulator Brassica napus cultivar (QY-1) grown at two mildly Pb-contaminated farmland sites surrounding working smelters in Jiyuan city, Henan province, China. Regardless of the frequency of the fertilizer treatments, the foliar application of WSC (0.01%) or Si (0.15%) significantly increased the QY-1 biomass and decreased the grain Pb concentrations. Compared with the control treatment, spraying plants once with WSC or Si during the flowering period achieved the best effect in the two soils with different pollution, which may be because inhibiting the accumulation of Pb in grains by decreasing the husk-to-grain transfer coefficient. Thus, the foliar application of WSC or Si combined with the cultivation of a low-Pb accumulator is a promising approach for optimizing the utility of Pb-contaminated farmland affected by atmospheric deposition.

Effects of Nano Chitosan and Bio Capsule on Growth, Yield and Quality of Chilli (Capsicum annuum L.) in Poly-House Condition

A field experiment was designed in a Randomized Block Design with 9 treatments replicated thrice on Chilli (Capsicum annuum L.) under poly-house condition. The Experiment examined the effect of nano chitosan, bio capsule and NPK @RDF application on growth, yield and quality of chilli and to work out the economics of various treatments. Various treatment combinations were prepared using different doses of Nano chitosan and Bio capusles in combination. It was observed that the treatment T8 (NPK + Nano chitosan 120 ppm + Bio capsule 120 ppm) was superior over other treatments in terms of growth, yield and quality of Chilli i.e., plant height (63.73 cm), fruit yield per 200m2 (3.27q), T.S.S. (9.67 0 Brix) and ascorbic acid (143.96 mg/100 g). Therefore, use of Nano chitosan @ 120 ppm along with 500 ppm Bio capsule could be used for getting better yield in chilli. Moreover, application of nano chitosan and bio capsule on nutritional quality of chilli could be studied in details further.

Chitosan Treatment Effectively Alleviates the Adverse Effects of Salinity in Moringa oleifera Lam via Enhancing Antioxidant System and Nutrient Homeostasis

Salt stress is a significant and common abiotic stress that negatively affects plant growth and development. Chitosan is a biodegradable and non-toxic stimulant of plant growth, which produces new routes to ameliorate the adverse effects of abiotic stresses. The possible profits of chitosan in salt stress alleviation have not been reported yet in Moringa oleifera, an important nutritional and medicinal tree. Therefore, the aim of the current study was to investigate the effect of chitosan treatment on salt-stressed moringa and its underlying physiological and biochemical mechanisms. Moringa plants were grown under 0, 25, 50 and 75 mM NaCl, while chitosan was applied at a 1% concentration as a foliar spray treatment. Growth attributes were considerably impaired, due to the salt stress treatment; however, chitosan application significantly reversed such an effect. Relative to the control, the fresh and dry weights of leaves were reduced by 51.44 and 48.74% in 75 mM-treated plants, while after chitosan treatment they were 33.61 and 35.72%, respectively. Additionally, chitosan treatment retarded chlorophyll and carotenoids reductions, enhanced the carbohydrate content, proline content, and phenol content and induced the activities of catalase, superoxide dismutase and ascorbate peroxidase in salt-stressed plants. Thus, chitosan application alleviated the oxidative injury, observed by lower malondialdehyde and H2O2 levels, thereby preserving membrane stability and improving antioxidant capacity and salt tolerance. In 50 mM NaCl-treated plants, chitosan treatment increased the activities of CAT, SOD and APX enzymes by 2.63, 2.74 and 2.31-fold relative to the control, respectively. Furthermore, chitosan application prevents the disturbance in ion homeostasis, and therefore not only enhanced the contents of N, P, K, Mg and Fe but also decreased Na content under salinity. Collectively, chitosan treatment overcame the adverse effects of salinity in moringa by activating the antioxidant machinery and preventing disturbance in ion homeostasis.

Oligochitosan fortifies antioxidative and photosynthetic metabolism and enhances secondary metabolite accumulation in arsenic-stressed peppermint.

The current study was designed to investigate whether application of irradiated chitosan (ICn), a recently established plant growth promoter, can prove effective in alleviating arsenic (As) stress in peppermint, a medicinally important plant. This study investigated how foliar application of ICn alleviated As toxicity in peppermint (Mentha piperita L.). Peppermint plants were treated with ICn (80 mg L−1) alone or in combination with As (10, 20, or 40 mg kg−1 of soil, as Na2HAsO4·7H2O) 40 days after transplantation (DAT), and effects on the growth, photosynthesis, and antioxidants were assessed at 150 DAT as stress severely decreases plant growth, affects photosynthesis, and alters enzymatic (ascorbate peroxidase, superoxide dismutase) and non-enzymatic (glutathione) antioxidants. When applied at 40 mg kg−1, ICn significantly decreased the content of essential oil (EO) and total phenols in peppermint by 13.8 and 16.0%, respectively, and decreased phenylalanine ammonia lyase (PAL) and deoxy-D-xylulose-5-phosphate reductoisomerase (DXR) activities by 12.8 and 14.6%, respectively. Application of ICn mitigated the disadvantageous effects caused by As toxicity in peppermint by enhancing activities of antioxidative enzymes and photosynthesis and increased accretion of secondary metabolism products (EOs and phenols). An enhancement of total phenols (increased by 17.3%) and EOs (36.4%) is endorsed to ICn-stimulated enhancement in the activities of PAL and DXR (65.9 and 28.9%, respectively) in comparison to the control. To conclude, this study demonstrated that foliar application of ICn (80 mgL−1) effectively promoted the growth and physiology of peppermint and eliminated As-induced toxicity to achieve high production of EO-containing crops grown in metal-contaminated soils.

Treatment of wastewater containing alizarin red dye: development and application of magnetic chitosan as a natural eco-friendly material

Treatment of wastewater containing alizarin red dye: development and application of magnetic chitosan as a natural eco-friendly material

Chitosan: A biopolymer for textile processes and products

Chitosan is a biopolymer obtained from deacetylation of the abundant natural chitin polymer. It has found its use in many applications due to its unique solubility as well as chemical and biological properties. In addition to its biodegradability and biocompatibility, it has many reactive amino side groups that offer the possibilities of chemical modification and formation of a large variety of beneficial derivatives. Numerous researchers have published works outlining the physical and chemical properties of chitosan, as well as its use in several industries, such as medical, environmental and food. Due to its excellent absorption and biological properties, chitosan present a myriad of opportunities in eco-friendly textile processes. Chitosan is able to confer antimicrobial, antiviral, anti-odor and other biological activities to textile fibers and fabrics. In this review, the first part is dedicated to developing the physio-chemical and biological characteristics of chitosan. Then, we present chitosan applications in the textile industry over the past decade. Literature works on chitosan usage along with different textile production steps (fiber formation, functional treatments, dyeing and effluent treatment) are extensively discussed.

Chemically Plasticized Chitosan Films by Grafting Succinic Acid: Surface Roughness and Mechanical Properties

A useful alternative to conventional plastic packaging manufactured from fossil fuels is packaging constructed of a biodegradable polymer. The final qualities of the chitosan cast films heavily depend on the type of plasticizer used and how it is incorporated into the chitosan-based material. This work investigates the synthesis of chitosan chemically grafted with succinic acid to improve the physical and mechanical properties of the film. The cortex of local shrimp was used to make a chitosan extract with a molecular weight of 2.702x105 g/mole and a deacetylation level of 80%. The chemically plasticized chitosan film was characterized by FTIR. It was found that chitosan and grafted chitosan film spectra were different from each other, implying the true grafting of succinic acid had occurred and the main backbone of the chitosan structure was unaffected. An examination of the mechanical properties of the films revealed that the results of the tensile strength measurement showed an improvement in the strength and %elongation at break for the chitosan grafted with succinic acid cast film for packaging, which is one of the potential applications for biodegradable chitosan.

Inhibition of Chitosan with Different Molecular Weights on Barley-Borne Fusarium graminearum during Barley Malting Process for Improving Malt Quality.

There are many Fusarium graminearum contaminations in barley that are often associated with malt and beer quality issues. Thus, it is important to find a biological antifungal agent to prevent the growth of F. graminearum during malting. Minimum inhibition concentration (MIC) of chitosan for mycelial growth and spore germination of F. graminearum was 2.6 g/L and 1.6 g/L, respectively, indicating that the F. graminearum strain was highly sensitive toward chitosan. Chitosan with a molecular weight of 102.7 kDa was added at 0.5 g/kg during the first steeping stage, resulting in the maximum inhibition rate of F. graminearm in barley. The biomass of F. graminearm and deoxynivalenol content in the infected barley at the end of germination with 0.5 g/kg chitosan treatment were decreased by 50.7% and 70.5%, respectively, when compared with the infected barley without chitosan. Chitosan could remove the negative effects of F. graminearm infection on barley germination and malt quality, which makes the application of chitosan during the steeping process as a potential antifungal agent in the malting process to protect from F. graminearum infection.

Elicitors induced changes in essential oil constituents of turmeric (Curcuma longa L.) rhizome

An experiment was conducted at IISR, Kozhikode to study the effect of foliar application of chemical elicitors, namely, chitosan (100, 200 and 500 ppm), phenylalanine (0.1, 1 and 10 mM) and salicylic acid (0.01, 0.1 and 1 mM) on volatile constituents of turmeric rhizome essential oil (EO). Three genotypes (Pragati, Rajapuri and Acc.849) which vary in growth duration and volatile profile were taken for the study in randomized block design with three replications. The highest EO content in Pragati (6%) and Acc. 849 (5.3%) was found in Phenylalanine (1 mM) treatment. No significant changes in EO content were observed in the genotype Rajapuri. Phenylalanine and salicylic acid were found to have positive influence on ar-turmerone, the major sesquiterpenoid in Pragati. Acc.849 and Rajapuri did not produce any significant changes to ar-turmerone content in elicitor treated samples. Moreover, the treatment related variation in the total monoterpenes and total sesquiterpene content was found significant among the genotypes. Multivariate analysis using partial least square discriminant analysis supported the variation observed among the treatments and variable importance in projection scoreidentified the metabolites responsible for variation among treatments.