Application Of Benzyladenine Research Articles

Processing browning and increasing multiplication and rotting of Peach rootstock cv. Garnem by using antioxidants and plant growth regulators in vitro

The study aimed to find an accurate propagation program for the rootstock of peach Prunus persica cv. Garnem. The apical shoots and nodes of this rootstock were used as explants for the stages of initiation and multiplication, and they were planted on MS medium after adding different concentrations of Active Charcoal (AC), Polyvinyl Pyrrolidone (PVP), and Benzyl adenine (BA). The shoots were planted for the purpose of rooting on MS medium containing different concentrations of salts (1/4 MS, 1/2 MS and full MS salts) with different concentrations of IBA. In the establishment phase, the apical shoots grown on medium supplied with AC were given the highest response rate, the highest average length of plantlet, and the highest number of leaves. The best multiplication of explants in terms of the number of shoots and their length were obtained when cultivated on a medium supplemented with BA at 1 and 2 mg.L-1, whereas the application of BA at 1 mg. L-1 to the cultivated nodes gave the highest number of leaves. The best rooting percent was obtained when plantlets were s treated with 2 mg. L-1 was applied to 1/4 MS medium. The highest number of roots was obtained from plantlets grown in 1/2 MS medium treated with IBA at 3 mg.L-1, whereas the longest root was obtained from plantlets grown in 1/2 MS medium treated with 1 mg.L-1IBA.

Seed Germination and Seed Vigour Induction through Foliar Application of Plant Growth Regulators and Nutrients under Drought Stress in Chickpea (Cicer arietinum L.)

Chickpea cultivation in rainfed regions, such as Madhya Pradesh, faces challenges due to water stress, impacting crop yield and quality. The study conducted assesses the impact of foliar spray with plant growth regulators and nutrients on the germination percentage, seedling length, and seed vigour indices of chickpea varieties (JG 36 and JG 14) under water deficit conditions. Cytokinin analogues, known for breaking dormancy, and other growth regulators and nutrients were applied during the flowering stage. The results revealed significant differences in germination percentage, seedling length, seed vigour index I, and seed vigour index II among irrigation levels, varieties, and foliar spray of plant growth regulators and nutrients. Under different irrigation levels, D1 (Irrigation at 30 DAS and at flower initiation) recorded the highest germination percentage (97.24%), seedling length (31.41 cm), seed vigour index I (3054.81), and seed vigour index II (52.77) compared to control D2 (Drought stress at Flowering up to physiological maturity). JG 14 (V1) performed well, achieving the highest germination percentage of 96.99%. Among the treatments, foliar application of Benzyladenine (BA) at 40 ppm (T3) resulted in the highest germination percentage (98.17%) and seed vigour index II (60.33), whereas T8 (BA 40ppm + ZnSO4 1%) exhibited the highest seedling length (31.83 cm) and seed vigour index I (3099.85). This study demonstrates that foliar spray with plant growth regulators and nutrients, particularly Benzyladenine at 40 ppm, significantly improved germination percentage, seedling length, and seed vigour index, offering potential solutions for enhancing chickpea productivity under water deficit conditions.

A Comparative Study of the Response of Fennel (Foeniculum vulgare, Mill) Plants from Egypt and China to Spraying with Benzyladenine (BA)

Fennel plant (Foeniculum vulgare, Mill.) is one of most widely used plants in food and medicine. Our study aimed to show the response of fennel plants from Egypt and China planted in Egypt to foliar application of benzyladenine (BA) at various concentrations; control, 50, 100 and 150 ppm. The obtained results indicated that treated fennel plants with BA resulted in a significant increase in the vegetative growth, number of umbels/plant, fruit, yield and oil yield per plant and feddan as well as main components, total phenolic contents and antioxidant activity of essential oils compared to untreated plants. In general, the application of benzyladenine was more effective on Egyptian fennel plants than that of Chinese plants. Egyptian fennel plants gave higher values of growth, fruit yield, oil percentage and oil yield than Chinese plants. However, the major components, TPC and antioxidant activity (DPPH %) had higher values in Chinese essential oil than Egyptian essential oil. The highest values of all studied parameters were obtained by using BA at 100 ppm. GC–MS analysis of the volatile oils showed that the major components were trans-anethole followed by fenshone, α- pinene and D-limonene, respectively for both essential oils. 100 ppm of BA gave the highest values of trans-anethole content and radical scavenging activities of oils. Therefore, we concluded that spraying fennel plants with 100 ppm BA can be used for obtaining higher vegetative growth, fruit and quantity and quality of oil.

The Effects of Growth Regulators and Apical Bud Removal on Growth, Flowering, and Corms Production of Two Gladiolus Varieties

Gladiolus is commonly propagated from corms. The multiplication rate of corms is low and to increase the propagation rate, we examined a combination of apical bud removal and the application of growth regulators. The experiments were conducted in two varieties, ‘Rose Supreme’ and ‘White Prosperity’, and over two seasons. The apical buds on the planting corms were either removed or left intact before the same corms were soaked in a suspension with either 100 ppm of benzyladenine (BA), 100 ppm of gibberellic acid (GA3), or pure water. The results showed that apical bud removal increased the number of corms and shoots. GA3 had limited the effect on corm and shoot production, but instead resulted in increased total leaf area and leaf weight per shoot. BA, on the other hand, increased the number of corms and shoots. Overall, the removal of the apical bud plus application of BA increased the number of corms and shoots but reduced the average corm diameter and leaf weight per shoot. This was clearer in ‘Rose Supreme’ than in ‘White Prosperity’. To maximize flower production for the coming season, farmers need to produce a high number of planting corms, but they also need to balance this with a sufficient corm size and the production of flowers of good quality. The application of growth regulators in combination with apical bud removal should be fine-tuned to avoid a situation that leads to the production of too many small or too few large corms.

Use of thinners can increase the fruit size of blueberries in an evergreen system

ABSTRACT In Australia, small and sour fruit can be a problem for growers of southern highbush blueberry (Vaccinium corymbosum L.) during the early season. A field experiment examined the impact of hand thinning and the foliar treatments, urea, ammonium thiosulphate (ATS), benzyladenine (BA) and ATS/BA combination on blueberry cultivar ‘OB1’ fruit quality for an entire season. Treatments were applied over four weeks from the first harvest. Urea (5%) and ATS (0.78%) were effective at desiccating open flowers. However, BA (0.015%) failed to thin fruit, applied alone or following ATS. Fruit size (berry weight and diameter), increased towards the end of the season without compromising yield for hand thinning, ATS, BA and ATS/BA. However, thinning had no effect on the concentration of total sugars or acids. Foliar urea, although an effective desiccant, had little effect on fruit size, and despite its use to reduce leaf senescence in autumn, it had no effect on the leaf canopy area. Future research should evaluate earlier applications of ATS and BA for increasing fruit size early in the season when fruit weight and diameter are most limited.

Plant Growth Regulator Impacts on Vegetative Cutting Production of Moroccan Pincushion (Pterocephalus depressus) Plants

Abstract Moroccan pincushion (Pterocephalus depressus) is a drought-tolerant perennial that is being used in landscapes throughout arid areas of the western United States. This paper describes two experiments researching vegetative cutting production from stock plants. Moroccan pincushion stock plants received foliar applications of gibberellic acid (GA3), benzyladenine, ethephon, or auxin [indole-3-butyric acid (IBA)] plant growth regulators (PGR). Plant growth regulators were applied singularly and in combination with GA3 to determine efficacy on stock plant growth. A propagation study was conducted simultaneously to determine effects of these different PGR treatments applied to stock plants on the rooting of moroccan pincushion cuttings. The stock plant study showed GA3 + benzyladenine application increased cutting production over other PGR treatments. Fresh weight of moroccan pincushion cuttings did not differ among treatments. While cuttings did not differ in dry weight in experiment 1, statistical differences were observed in experiment 2. However, these differences in dry weight did not affect the quality of the cuttings. Cuttings from stock plants treated with GA3 + IBA treatment had the highest numerical growth index [(height + width + width)/3]. Cuttings from stock plants treated with GA3 alone or in combination with another PGR were all greater in average growth index and statistically differed from those without GA3 being applied. PGR treatments did not affect rooting percentages of the cuttings with nontreated stock plant cuttings successfully rooting at an average rate of 95%. However, GA3 + IBA was the only treatment where cuttings had 100% rooting for both experiments, indicating potential rooting benefits. Index words: Plant growth regulator, propagation, Pterocephalus depressus, vegetative cuttings. Species used in this study: Moroccan pincushion [Pterocephalus depressus Archibald]. Chemicals used in this study: gibberellic acid (GA3), benzyladenine, ethephon, indole-3-butyric acid (IBA).

Moniliophthoraperniciosa, the causal agent of witches’ broom disease of cacao, interferes with cytokinin metabolism during infection of Micro‐Tom tomato and promotes symptom development

Moniliophthora perniciosa causes witches' broom disease of cacao and inflicts symptoms suggestive of hormonal imbalance. We investigated whether infection of the tomato (Solanum lycopersicum) model system Micro-Tom (MT) by the Solanaceae (S)-biotype of Moniliophthora perniciosa, which causes stem swelling and hypertrophic growth of axillary shoots, results from changes in host cytokinin metabolism. Inoculation of an MT-transgenic line that overexpresses the Arabidopsis CYTOKININ OXIDASE-2 gene (35S::AtCKX2) resulted in a reduction in disease incidence and stem diameter. RNA-sequencing analysis of infected MT and 35S::AtCKX2 revealed the activation of cytokinin-responsive marker genes when symptoms were conspicuous. The expression of an Moniliophthora perniciosa tRNA-ISOPENTENYL-TRANSFERASE suggests the production of isopentenyladenine (iP), detected in mycelia grown in vitro. Inoculated MT stems showed higher levels of dihydrozeatin and trans-zeatin but not iP. The application of benzyladenine induced symptoms similar to infection, whereas applying the cytokinin receptor inhibitors LGR-991 and PI55 decreased symptoms. Moniliophthora perniciosa produces iP that might contribute to cytokinin synthesis by the host, which results in vascular and cortex enlargement, axillary shoot outgrowth, reduction in root biomass and an increase in fruit locule number. This strategy may be associated with the manipulation of sink establishment to favour infection by the fungus.

The Morphological Responses of Calendula officinalis L. “Radio” to the Foliar Application of Benzyladenine and Different Light Spectra

Pot marigold is a valuable medicinal plant with great decorative value. Three combinations of light (white (W)—170 μmol m−2 s−1, white + blue (W+B)—230 μmol m−2 s−1, white + red (W+R)—230 μmol m−2 s−1) were used to analyse the influence of a diversified light spectrum on the morphological traits and flowering of Calendula officinalis L. “Radio”. The effect of foliar treatment of the plants with 6-benzyladenine (BA) at concentrations of 100, 150 and 200 mg dm−3 at all the light spectrum combinations was analysed. BA had negative influence on the earliness of florescence and delayed it even by more than 10 days. W+B light intensified the delay, whereas red light partly reduced it. The BA treatment had the greatest influence on the biometric traits of the plants at the initial period of their development. W+B light significantly inhibited the growth of the plants. A high share of red light in the spectrum positively affected the Fv/Fm value, the relative chlorophyll content and the percentage of dry matter in the plants. When the amount of blue or red light in the spectrum increased, it was possible to obtain specific biometric traits of Calendula without the BA treatment.

Influence of Foliar Application by Benzyladenine and Yeast Extract on Growth, Root Yield, Quality and Chemical Compositions of Sugar beet (Beta vulgaris L.) Plant

Two field experiments were carried out at the Experimental Research Station, Faculty of Agriculture, Moshtohor, Benha University, Qalyoubia Governorate, Egypt, during 2016/2017 and 2017/2018 seasons, to study the effect of foliar application of benzyladenine (BA at 50 and 100ppm) and yeast extract (at 100 and 200 ml/L) in which were applied three times (50, 70 and 90 days after sowing) on growth, root yield characteristics and chemical compositions of sugar beet plant (Beta vulgaris L. cv. Ras-Poly). The obtained results indicated that, the foliar application with different applied treatments of both benzyladenine and yeast extract significantly increased all vegetative growth characteristics of roots (i.e. root length, diameter, fresh and dry weights/ plant) and leaves(i.e. leaves number, total leaves area/ plant and fresh and dry weights/ plant) at 110 days after sowing).The results also indicated that the foliar application of these treatments significantly increased root yield characteristics and quality at harvest time compared with control plants in both seasons. Data also showed that, foliar spraying of BA at 100ppm and yeast extract at 200 ml/L increased photosynthetic pigments, minerals contents, total carbohydrates and crude protein content in sugar beet leaves at 110 days after sowing. Results also gave the highest values of all studied vegetative growth and root yield characteristics as well as root quality in this study. Hence, it could be concluded that foliar application of benzyladenine (BA) at 100 ppm and yeast extract at 200 ml/L have promoting effect on growth, root yield and quality of sugar beet plants.

Combined effects of NPK fertilizer with foliar application of benzyladenine or gibberellic acid on Dracaena marginata ‘Bicolor’ grown in different potting media

Abstract A pot experiments was carried out to evaluate the influence of growing media and combined treatments of NPK with either benzyladenine or gibberellic acid on growth, chemical constituents and anatomical structure of Dracaena marginata ‘Bicolor’. The plants were grown in two growing media; peat-moss, peat-moss+ sand (1:1, v v-1), received monthly NPK fertilizers (2 and 4 g pot-1) combined with either of benzyl adenine (BA) at 100 and 150 ppm or gibberellic acid (GA3) at 150 and 250 ppm, while the control plants received no treatments. As general, the results indicated that, peat- moss was superior to peat-moss+ sand medium on increasing most of vegetative growth parameters in terms of plant height, number of leaves/plant, leaf area, root length, as well as fresh and dry weights of leaves, stems and roots/plant, besides some macro elements represented in N, P, K, Ca and Mg% in both leaves and stems. While plants grown in peat-moss+ sand possessed significantly higher contents of total chlorophylls, total carbohydrates, Cu, Fe, Mn, Zn and B than those grown in peat- moss alone. Plants received combined NPK with either BA or GA3 resulted in significant increases in most of morphological and chemicals content over the control plants and it was outstanding that, GA3 was more effective than BA when they were combined with NPK. It can be concluded that for the highest quality, quantity growth and economic production of Dracaena marginata ‘Bicolor’, the plants could be grown in a medium of peat-moss and supplied monthly with NPK fertilizer at 2 g plant-1 along with foliar sprayed with 250 ppm GA3.

APPLICATION OF BENZYLADENINE (BA) IN THE FORM OF LANOLIN PASTE ON FLOWER-STALK BUDS SUCCESSFULLY INDUCED RE-BLOOMING OF HYBRID Phalaenopsis

Hybrid moth orchid (genus Phalaenopsis) is one of the most popular ornamentals in Indonesia. It has beautiful and long-lasting flowers, but cultivating this orchid is still become a challenging issues due to the need of specific condition to grow and long time period to re-blooming. Plant growth regulators (PGR) (i.e. Benzyladenine (BA)) has been widely documented as a flower-inducing substance in several orchids. However, the optimal concentration and its mechanism in inducing flower-stalk bud and re-blooming is still unclear. This research aimed to study the effects of BA application in the form of lanolin paste on hybrid Phalaenopsis flower-stalk buds. We conducted this study using completely randomized design with four replications at the greenhouse laboratory Faculty of Agriculture University of Lampung on August to December 2018. We divided the orchid into 5 group of BA concentration (0, 1000, 1500, 3000, or 6000 ppm). The sheath of the fourth or fifth buds of the flowers were carefully opened, then it smeared with BA. The percentage of bud break into flower spike or keiki, length of shoots or spike and number of open flowers were recorded until 10 weeks of observation. The results showed that, neither of the buds under the control treatment (without BA), 1000 ppm nor 1500 ppm BA broke and grew into keiki or spike. On the other hand, application of BA at 3000 ppm or 6000 ppm successfully induced 100% flower spikes on the buds treated. No keiki was formed in all buds treated. In addition, treatment of the buds with 6000 ppm BA produced longer flower spikes as well as more open flowers. We conclude that the application of BA (minimal 3000 ppm) successfully induced flower spike of hybrid Phalaenopsis.

THE EFFECTS OF PRETREATMENT FACTORS ON SEED GERMINATION AND SEEDLING GROWTH OF ANISE (PIMPINELLA ANISUM L.)

Seed dormancy is one of the major problems in agricultural studies, especially for medical plants. Anise ( Pimpinella anisum L.) is an important medical plant with dormant seed and it is established and distributed only in its natural habitats. In order to evaluate the effects of some pretreatment factors on primary growth and germination characteristics of Anise, an experiment was conducted as Factorial layout within completely randomized design with four replications. Pre-chilling treatments were 0, 15, 30 and 45 days treatments and hormone treatments were GA 3 (Gibberellic Acid), BA (Benzyladenine), KI (Kinetin), GA 3 +BA, GA 3 +KI, BA+KI, GA 3 +BA+KI, KNO 3 , H 2 SO 4 and distilled water as a control treatment. Prechilling treatment effects on coleoptile and radicle length, seedling length, germination percentage, mean time for germination, germination rate and seed vigor index were meaningful. Different hormone treatments had significant influence on coleoptile and radicle length, seedling length, germination percentage, mean time germination, germination rate and seed vigor index. The highest germination percentage and germination rate was related to usage of BA+KI. The higher values for radicle length and uniformity of seed germination were achieved in application of BA and KI, respectively. Moreover, application of GA 3 +BA+KI had obtained the highest seed vigor index. It seems that application of endogenous GA 3 +KI and BA+KI concentration, which is provided mostly by chilling treatment, is the most effective factor for breaking the seed dormancy. On the basis of the results, usage of 45 days moist prechilling accompanied with application of GA 3 +KI and BA+KI in Esfahan cultivar was appropriate.

GROWTH BEHAVIOR AND PRODUCTIVITY OF LEMONGRASS (CYMBOPOGON CITRATUS) AS AFFECTED BY FOLIAR APPLICATIONS OF VARIOUS PROMOTING

This work was performed during two successive growth seasons (2013 and 2014) at the Experimental Farm, Faculty of Agriculture, Menoufia University, Shebin El – Kom , Egypt to study the individual and combined effects of foliar application of benzyl adenine (BA) at (0, 50 and 100 mg/l) and mixture of some micronutrients (iron, manganese, zinc and boron) as a compound chelated (EDTA form) at different levels on growth, yield and active constituents of lemongrass plant (Cymbopogon citratus L.) grown on a clayey soil. Micronutrients were sprayed at different concentrations, i.e., M0 = irrigation water without any of micronutrients, M1= 100, 50, 50 and 25 mg/l, M2 = 150, 100, 100 and 50 mg/l, M3 = 200, 150, 150 and 75 mg/l for Fe, Mn, Zn and B, respectively. The experiment was carried out in a split plot design with three replicates. Results indicated that, the combinations between different rates of BA and mixture of micronutrients had a significant improvement effect on growth and yield characters in most cases expressed as plant height (cm), number of tillers/plant, fresh and dry weights of herb (g/plant) and fresh weight of herb (ton/fed) in the 1st and 2nd cuts during the two seasons. Their highest values were obtained in the plants treated with BA at 100 mg/l combined with M2 level of micronutrients in the two cuts through the first and second seasons. The highest oil percentages were obtained by using BA at 50 mg/l + M2 level of micronutrients and BA at 100 mg/l + M1 level of micronutrients for the first and second cuts respectively during the two seasons. Citral a (geranial), citral b (neral) and myrcene were found to be the major compounds in the essential oil of Cymbopogon citrates which reached its maximum values by using BA at 100 mg/l + M1 level of micronutrients, BA at 50 mg/l + M2 level of micronutrients and M1 level of micronutrients without using BA respectively, during the 2nd cut in the first season.

Growth and Flower Bud Induction in Strawberry ‘Sulhyang’ Runner Plant as Affected by Exogenous Application of Benzyladenine, Gibberellic Acid, and Salicylic Acid

Strawberry (Fragaria × ananassa) is one of the most important and popular fruit crops in the world, and ‘Sulhyang’ is one of the principal cultivars cultivated in the Republic of Korea for the domestic market. The growth and flower induction in strawberry is the process which influences directly on fruit bearing and yield of this crop. In this study, effect of benzyladenine (BA), gibberellic acid (GA3), and salicylic acid (SA) on growth and flower bud induction in strawberry ‘Sulhyang’ was investigated. The 3-week-old runner plants, grown in 21-cell propagation trays, were potted and cultivated in growth chambers with 25oC/15oC (day/night) temperatures, 70% relative humidity (RH), and light intensity of 300 μmol·m-2·s-1 photosynthetic photon flux density (PPFD) provided by white light emitting diodes (LEDs). The runner plants were treated with one of three concentrations, 0 (control), 100, and 200 mg·L-1 of BA, GA3, or SA solution. The chemicals were sprayed two times on leaves of runner plants at an interval of two weeks. After 9 weeks the results showed that the application of all chemicals caused reduction of root length and chlorophyll (SPAD) content as compared to the control. The lowest chlorophyll (SPAD) content was recorded in plants treated with GA3. However, the treatment of 200 mg·L-1 GA3 promoted leaf area, leaf fresh weight, and plant fresh weight. The greatest flower induction (85%) and number of inflorescences (4.3 inflorescences per plant) were observed in the treatment of 200 mg·L-1 SA, followed by 100 mg·L-1 SA. Overall, results suggest that foliar application of GA3 solution could accelerate plant growth, while foliar application of SA solution could induce hastened flowering. Further studies may be needed to find out the relationship between GA3 and SA solutions treated in a combination, and the molecular mechanism involved in those responses observed.

Assessment of GA3 and BA application on gerbera cultivation in soilless culture

This research was carried out to determine the effect of gibberellic acid (GA3) and benzyladenine (BA) application on yield and quality components (multiplication ratio, flower stem length, flower diameter, vase life, flower stem diameter,) of gerbera. Three different levels of GA3 (125, 250, 500 ppm) and BA (100, 200, 400 ppm) were applied by foliar spray. Compared to BA application, GA3 applications gave good results on yield and quality parameters. The maximum yield was obtained by 125 ppm of GA3 application. It was found that 500 ppm of GA3 application most effective on stem length and flower diameter. On the other hand, it's determined that 400 ppm BA application significantly increased stem diameter and multiplication ratio. Its concluded that, the GA3 and BA demonstration interference in growth characteristics of gerbera plant.

Interaction of Phytohormones in Regulating the Axillary Bud Growth in Pea

In the present study the interactions of GR24, a synthetic analog of newly discovered plant hormones strigolactones (SLs), with cytokinin (CK), benzyladenine (BA), auxin naphthaleneacetic acid (NAA), gibberellic acid (GA3) and abscisic acid (ABA) in the regulation of axillary bud growth in pea (Pisum sativum L.) were investigated. The hormones were applied directly to buds at node 1 and 2 and the dose-response experiments were performed on 8–10-day-old SL-deficient rms1 and rms5–1 mutants, branching SL-sensitive rms2–1 mutants and wild-type plants. In the mutant plants the treatment with 5 μM GR24 completely inhibited bud growth while BA up to 100 μM stimulated it. The combined application of GR24 and BA showed that GR24 efficiency to reduce bud outgrowth constantly declines as CK-stimulated bud growth increased, with the inhibiting effect of GR24 abolished at 100 μM BA applied. GA3 accelerated bud outgrowth, but did not interfere with GR24 inhibitory action. NAA reduced bud growth in intact SL-sensitive rms2–1 mutant and also in SL-insensitive rms3–2 and rms4–1 mutants. The NAA effect was observed already at 0.5 μM, however, even at a response saturating concentration of 500 μM its inhibiting effect was inferior to that of 5 μM GR24. At combined treatment the effectiveness of GR24 in suppressing bud growth decreased with a decrease in NAA-inhibited bud growth, suggesting that auxin might act as a suppressor of SL action. ABA strongly inhibited the bud outgrowth and, like CK and auxin, reduced the inhibitory effectiveness of GR24. The revealed ability of CK, ABA and auxin to suppress bud response to SLs is supposed to result from phytohormone signaling crosstalks.

Benzyl adenine is more effective than potassium silicate on decreasing the detrimental effects of heat stress in pepper (Capsicum annum cv. PS301)

ABSTRACT- Heat stress causes flower and fruit abscission in pepper. This study was conducted in the greenhouses of Isfahan University of Technology to evaluate the effect of foliar application of Benzyl adenine (BA) and potassium silicate (K2SiO3) under heat stress condition on bell pepper. Two factorial experiments based on completely randomized design with four concentrations of BA (0, 0.06, 0.6 and 6 ppm) and the second with two levels of K2SiO3 (0 and 5 Mm) both in two temperature treatments (25±2 (optimum) 35±2 (high temperature)) with six replicates were conducted. The results of the study indicated that the use of BA (especially 6 ppm) promoted growth parameters and increased proline, phenol and antioxidant content. Also, application of BA 6 ppm improved cell membrane stability assessed via decreasing electrolyte leakage (EL) and also reduced flower abscission in bell pepper. BA at 6 ppm increased plant height, shoot and root dry weight, proline and total phenol, root fresh weight, potassium (K) concentration and decreased flower abscission. Antioxidant content increased with heat stress in all BA levels. Results of the study indicated that fresh and dry weight of root and K concentration increased with 5 mM K2SiO3. Moreover, root fresh weight and K concentration and antioxidant content increased in 5 mM K2SiO3 under heat stress.

Response of False Aralia Plant to Gibberellic Acid and Benzyladenine Application

False aralia (Dizygothecaelegantissima) also known as spider aralia or threadleaf aralia, is grown for its attractive foliage. The long, narrow, dark green leaves with saw-tooth edges are coppery colored at first, but as they mature they turn dark green, appearing almost black on some plants. Field trials with False aralia (Dizigotheecaelegantissima) were conducted at the experimental greenhouse of Faculty of Agriculture, Azad University Jiroft in growth seasons of 2013. The aim of this work was to study the effect of foliar application of gibberellic acid (GA3) and benzyladenine (BA) both at 0, 100 and 200mgL-1 on the growth and photosynthetic pigments of Dizygothecaelegantissima plant. Effect of GA3and interaction significant (p<0.01) also effect BA on leaf area was non significant. Results showed that, 200 mg L-1 GA3+ 200 mg L-1 BA increased leaf area of False aralia as 43.87% compared to control treatment. 200 mg L-1 GA3 + 200 mg L-1 BA increased chlorophyll index, plant height and leaf area of False aralia as 61.62, 24.48 and 43.87% compared to control treatment. Also 200 mg L-1 BA cause increased chl. (b), total chl. a+b and sum pigments of False aralia as 15.65, 38.03 and 36.91% compared to control treatment.

Antioxidant capacity and pigment synthesis of marigold (&lt;i&gt;Calendula officinalis&lt;/i&gt; L.) as influenced by benzyladenine and epibrassinolide

The impact of various rates of benzyladenine (BA) and epibrassinolide (EPL) was explored on antioxidant capacity and pigment biosynthesis of marigold in a factorial experiment on the basis of a Randomized Complete Block Design with two factors including benzyladenine (BA) and epibrassinolide (EPL), both at four rates of 0, 1, 5 and 10 mg/L with three replications. Means comparison revealed that the highest antioxidant capacity was devoted to the treatment of “0 mg/L BA × 5 mg/L EPL”, the highest flavonoid concentration at three wavelengths of 270, 300 and 330 nm was observed in the treatment of “10 mg/L BA × 10 mg/L EPL”, and the highest anthocyanin was obtained from the treatment of “1 mg/L BA × 0 mg/L EPL”. According to results, it can be said that the application of BA and EPL significantly influenced catalase, peroxidase, phenol, chlorophyll a, anthocyanin, and carotenoid in marigold plants.

Pengaruh Jenis Pupuk dan Konsentrasi Benzyladenin (BA) Terhadap Pertumbuhan dan Pembungaan Anggrek Dendrobium Hibrida

Dendrobium as ornamental plants that are used as cut flowers, potted plants, wreaths, garden ornament has a high economic value (due to the type, beauty, shape, texture, flower size, and rarity), has a broad market opportunities, sources of germplasm as mains to cross in order to get new varieties, and to cultivate them do not require large tracts of land. to get orchid production which is reached with good cultivation, one of them is by using fertilizers and growth regulators (PGR) to obtain growth and good flowering of orchids. This study was conducted to answer the following questions: (1) Which type of foliar fertilizer that can generate the best response to the growth and flowering of orchid Dendrobium, (2) how the effect of benzyladenine (BA) on the growth and flowering of orchid Dendrobium, (3) whether there is an interaction between foliar fertilizers and provision of benzyladenine (BA) to influence the growth and flowering of orchid Dendrobium. The research conducted in the greenhouse, Faculty of Agriculture, University of Lampung from January to June 2014. The experiments were carried out with a complete randomized block design with three replications. The treatments arranged in factorial (2x5). The first factor of 2 types of fertilizers, was (NPK 20-15-15) and (NPK 10-40-15). The second factor is the various concentrations of BA are: 0, 100, 200, 300, and 400 mg/l. based on data and discussion of experimental results that have been raised, it can be concluded as follows: Gandasil fertilizer (NPK 20-15-15) and Hyponex (NPK 10-40-15), had no effect on the growth and flowering of plants Dendrobium hybrids indicated by a variable percentage of plants to germinate, the number of new shoots, the height of new shoots, the increase of leaf pseudo stem diameter, and the percentage of flowering plants. Application of benzyladenine (BA) on the concentration of 100-400 mg / l, can stimulate flowering orchid hybrid Dendrobium, which is indicated by an increase in the percentage of flowering from 60.50 to 64.83%. Application of BA on the concentration of 100-400 mg / l had no effect on the percentage of ornamental plants whis have new sprout, and the number and the height of new shoots. There is no interaction between the two types of NPK (20-15-15 and 10-40-15), with concentrations of BA in influencing all observed variables. Keywords: Dendrobium, Two kinds of completed fertelizers, Benzyladenine, flowering