Antioxidant capacity and pigment synthesis of marigold (<i>Calendula officinalis</i> L.) as influenced by benzyladenine and epibrassinolide

Abstract

The impact of various rates of benzyladenine (BA) and epibrassinolide (EPL) was explored on antioxidant capacity and pigment biosynthesis of marigold in a factorial experiment on the basis of a Randomized Complete Block Design with two factors including benzyladenine (BA) and epibrassinolide (EPL), both at four rates of 0, 1, 5 and 10 mg/L with three replications. Means comparison revealed that the highest antioxidant capacity was devoted to the treatment of “0 mg/L BA × 5 mg/L EPL”, the highest flavonoid concentration at three wavelengths of 270, 300 and 330 nm was observed in the treatment of “10 mg/L BA × 10 mg/L EPL”, and the highest anthocyanin was obtained from the treatment of “1 mg/L BA × 0 mg/L EPL”. According to results, it can be said that the application of BA and EPL significantly influenced catalase, peroxidase, phenol, chlorophyll a, anthocyanin, and carotenoid in marigold plants.