Application Of Barcoding Research Articles

Development of Standard Components of SMAN 1 Lingsar Library Based on National Library Standards

School library management with conventional systems is time-consuming and ineffective. This study aims to develop a development model for SMAN 1 Lingsar library standard components based on SNP. The method used is research and development with observation and FGD as data collection techniques. The results include the addition of book collections from student grants, improvement of facilities and infrastructure, optimization of services with barcode applications, technical training, funding from the West Lombok Regency Library, and development through book review competitions and automatic inventory. Suggestions are provided for implementing this model to meet school library standards.

Application of DNA Barcoding to Identify Medicinal Plants.

Medicinal plants are valuable resources globally and are used worldwide to maintain health and treat disease; however, the presence of adulteration obstructs their development. DNA barcoding, a technique for species identification by standard DNA regions, facilitates prompt and accurate identification of traditional medicinal plants. The process of DNA barcoding entails six basic steps: 1) processing the medicinal plants, 2) extracting high-quality total DNA from the medicinal plants using centrifugal column method, 3) amplifying target DNA region internal transcribed spacer 2 (ITS2) with universal primers of plants and performing Sanger sequencing, 4) splicing and aligning sequence to obtain the target sequence, 5) matching the barcode sequence against the barcode library for identification, 6) aligning sequence, comparing intraspecific and interspecific variation, constructing phylogenetic neighbor-joining tree. As shown in the results, the universal primer can amplify the target region. Basic Local Alignment Search Tool (BLAST) demonstrates the percentage identified was 100%, and the neighbor-joining tree demonstrates that the splicing sequences were clustered with the A. sinensis OR879715.1 clade, and the clade support value is 100. This protocol provides a reference for applying DNA barcoding technology as an effective method to identify medicinal plants and adulterants.

Mitochondrial DNA sequence-based identification of two subterranean termite species, from Riyadh Province, Kingdom of Saudi Arabia.

Termites are economically important wood-destroying and agricultural pests. The termite fauna almost consists of 2900 described species in 286 genera worldwide. In the present study, hundreds of termite samples from 42 different locations in the Riyadh province were collected. These samples were previously used for morphometric identification and reported two subterranean termite species, Coptotermes heimi and Psammotermes hypostoma, in the family Rhinotermitidae. In the present study, these samples were analysed using DNA barcoding with the mitochondrial cytochrome c oxidase subunit 1 gene to confirm the conventional taxonomical identification on a molecular basis. The obtained COI gene sequences of all 42 termite specimens were submitted to GenBank (accession numbers: ON529959-ON529969, OP825131-OP825132, and OP890882-OP890910). Eleven of the 42 samples were thus identified as C. heimi and the remaining 31 samples as P. hypostoma, which were phylogenetically analysed. All the 11 C. heimi sequences were grouped in a single clade, indicating close relatedness. While 31 sequences of P. hypostoma constituted two clades in the phylogenetic tree. Pairwise nucleotide sequence identity and divergence analysis showed that C. heimi sequences showed high nucleotide identities of 87.6-99.5% and less divergence ranging from 0.5% to 13.6%. Similarly, sequences of P. hypostoma also showed high nucleotide identity of 78.6-100% and low divergence among them ranging from 0-10.7%. A further application, significance, and shortcomings of COI-based DNA barcoding have been discussed. DNA barcoding using the COI gene is a reliable tool to distinguish C. heimi and P. hypostoma genotypes.

Combating Illegal Trade of Monitor Lizards (Varanus spp) in Malaysia through DNA Barcoding Applications

Abstract Varanids, known as monitor lizards are traded as pet animal commodities and demand for skin trade especially in Southeast Asian countries. Given the significant demand in the international trade, accurate species identification is imperative for Varanids particularly endangered species to prevent misidentification and fraud in marketplaces as Varanids species exhibit morphological similarities. Therefore, advanced molecular technique is vital to facilitate accurate species identification especially for closely related species. Besides, few research have used cytochrome oxidase subunit I (COI) to track the genetic and phylogenetic analysis of Varanids. In this study, the COI mitochondrial DNA region was tested as a molecular marker for species identification of Malaysia monitor lizards using the DNA barcode. Two species were successfully identified from eight samples from Pantai Penyabong, Mersing, Johor which are V. salvator and V. nebulosus based on phylogenetic analysis (Neighbor-Joining, Maximum Parsimony and Bayesian Tree). This study showed that by using their relative genetic divergence and evolutionary links, DNA barcodes can aid in the species identification through molecular technique. Short sequences can be used to identify closely related species, and the possibility of species misidentification among the study’s samples should be taken into account. DNA barcoding techniques should be widely practised especially in wildlife forensics to facilitate precise wildlife identification and improve the efficiencies in combatting illegal wildlife trade in Malaysia.

The Situation of Counterfeited and Mislabeled Commercialized Edible Mushrooms in China and the Development of Possible Controls

Edible mushroom products, encompassing both cultivated and wild varieties, are highly favored by consumers due to their rich nutritional profiles, including significant levels of proteins and amino acids. These mushrooms have extensive applications across the food, pharmaceutical, and cosmetic industries, making the edible mushroom industry a vital component of global poverty alleviation efforts. Taking China as an example, the country produces over 45 million tons of edible mushrooms annually, accounting for 94.01% of the world’s total production, thereby establishing itself as the leading global producer of edible mushrooms. However, alongside the rapid expansion of this industry, concerns have emerged regarding counterfeit products and incidents of poisoning resulting from the consumption of toxic wild mushrooms. As follows, to advance the development and integrity of the mushroom production and processing industry: (1) This study presents the situation of counterfeit edible mushrooms and elucidates the factors contributing to the production of fraudulent products from both subjective and non-subjective perspectives. (2) We provide a detailed introduction to 22 varieties of freshly cultivated edible mushrooms and commonly encountered wild edible mushrooms in the Chinese consumer market, proposing the application of DNA barcoding, environmental DNA analysis, and other technologies for the future authentication of counterfeit mushroom products. (3) Concurrently, we present an overview of mushroom poisoning incidents in China from 2010 to 2023, emphasizing the challenges in mitigating the risks associated with wild mushroom consumption and preventing food poisoning, thereby necessitating heightened consumer caution. (4) Finally, we offer four recommendations aimed at ensuring the healthy, stable, and sustainable growth of the edible mushroom industry.

Investigating the antioxidant and anticancer potential of Daucus spp. extracts against human prostate cancer cell line C4-2, and lung cancer cell line A549

The study evaluated 297 carrot germplasm lines, focusing on 52 cultivars to explore their therapeutic potential and address challenges related to the accessibility and affordability of nutraceuticals and health promoting foods. The investigation explores the application of DNA barcoding using the ITS region for precise species identification, highlighting genetic diversity among the examined cultivars. Through ITS sequence-based analysis and phylogenetic examination, six diverse Daucus spp. genotypes were differentiated and classified into distinct groups, indicating the presence of vast genetic variation. Evaluation of antioxidant activities using the DPPH radical scavenging assay revealed varying degrees of scavenging ability among genotypes with SKAU-C-15, SKAU-C-17, and SKAU-C-16 exhibiting the highest activity, suggesting their potential for antioxidant-rich products. Thin Layer Chromatography (TLC) bioautography confirmed the presence of bioactive compounds in carrot extracts responsible for their antioxidant properties. In cell culture studies, specific carrot genotype extracts demonstrated potential anti-proliferative and anti-invasive effects on recurrent prostate cancer cell line - C4-2 (SKAU-C-30, SKAU-C-10, and SKAU-C-42) and non-small cell lung cancer cell line - A549 (SKAU-C-18 and SKAU-C-11) cancer cells, as indicated by MTT assay, wound healing assay, and Colony Forming Unit assay. These findings suggest the promising therapeutic potential of carrot genotypes for developing anti-cancer functional foods, nutraceuticals and health supplements.Therefore, the study contributes to the nutrition security, paving the way for advancements in functional foods and health applications, particularly in cancer treatment and prevention.

New Variants in the Chloroplast Genome Sequence of Two Colombian Individuals of the Cedar Timber Species (Cedrela odorata L.), Using Long-Read Oxford Nanopore Technology

The plant species Cedrela odorata has been largely exploited in the timber industry due to the high demand for its wood. Therefore, C. odorata has been considered a vulnerable species since 1994, under the Convention on International Trade in Endangered Species of Wild Fauna and Flora (CITES). C. odorata is a key timber species included in the management and conservation plans for the Amazon and Central American rainforests. These plans include the development of genetic and genomic resources to study local populations of the species in Colombia. In this study, two novel chloroplast (cp) genomes were assembled and annotated using the MinION long-read sequencing technology. The new cp genomes were screened for sequence variants (SVs), and a total of 16 SNPs were identified, presumably unique to populations from the Amazon region in Colombia. Comparative genomics with other reported cp genomes from different populations of C. odorata support the hypothesis of intraspecific diversity associated with their place of origin. These cp genome sequences of C. odorata from Colombian individuals represent valuable genomic resources for the species, suitable for identifying novel DNA fingerprinting and barcoding applications.

Application of DNA barcode for the genetic analysis and identifying a May nuoc mo species in Quang Nam Province, Central Vietnam

Abstract. Hieu HK, Minh NV, Loi NV, Ha HT. 2024. Application of DNA barcode for the genetic analysis and identifying a May nuoc mo species in Quang Nam Province, Central Vietnam. Biodiversitas 25: 3299-3308. Precise identification in Calamus, the genus in the subfamily Calamoideae (Arecaceae), is challenging due to taxonomic complexities. This study aimed to discriminate and identify the 23 May nuoc mo samples collected from Quang Nam, Vietnam using four barcoding regions (rbcL, matK, psbA-trnH, and ITS nrDNA) and combined with morphological characters. DNA sequence data from those regions were analyzed using distance, tree, and similarity-based statistical methods. All region sequences presented no variable sites, except for rbcL gene region with a low rate of nucleotide differences of 0.270%. The results of the 23 May nuoc mo samples were highly similar from 99.73 to 100% to Calamus sp. N_XT142 (ON248649.1 and MK692404.1) and no gene regions were proposed as barcodes for species identification in this study. All samples shared certain morphological characteristics, including leaflets arranged regularly but with gaps; petioles with yellow spine groups; and leaf sheaths with hairs, densely arranged with brown, triangular, and flattened spines, and interspersed among many short, and black spines. Based on these results, we recommend using some other gene regions (rpoC, and rpoB)) in species identification to shed more light on these May nuoc mo samples with subsequent studies.

Applications and challenges of DNA barcoding in rapid radiation groups: Rhodiola (Crassulaceae) as a case study

ObjectiveRhodiolae Crenulatae Radix et Rhizoma (Hongjingtian in Chinese, RCRR), the roots and rhizomes of Rhodiola crenulata and its application in the medicinal market is very chaotic. In this study, DNA barcoding database and identification engine of Rhodiola species were established, decoction pieces from the medicinal market were identified, and the application and challenges of DNA barcoding in the rapid radiation of Rhodiola species were analyzed. This study provides reference for the protection, rational development, and utilization of endangered resources within Rhodiola species. MethodsA total of 50 original plant samples from 20 species of the genus Rhodiola from Hebei, Xinjiang, Tibet, Jilin, and other major production areas were collected. Theses samples cover the typical distribution area (Qinghai-Tibetan Platea) of Rhodiola species and other scattered alpine regions (Changbai Mountain, Taibai Mountain, Lushan Mountain, etc.), it encompasses all Rhodiola species with thick rhizomes in China. ITS2 and psbA-trnH barcode of Rhodiola database (BORD) were established and an identification engine named Rhodiola-IDE was developed. The stability and accuracy of the standard DNA barcoding database were evaluated using two datasets. Rhodiola-IDE identified 31 decoction pieces of RCRR from the medicinal material market. ResultsThe BORD containing 1 532 sequences of 88 Rhodiola species has been established, and the identification efficiency results showed good accuracy and stability. According to the Chinese Pharmacopoeia (2020 edition), 23 samples (74.2%) were identified as authentic R. crenulata, while the rest of the marketed varieties were R. kirilowii, R. dumulosa, and R. fastigiata. The product label “Larger flower, Hongjingtian” was identified as R. crenulata. Samples labeled as “Smaller flower, Hongjingtian” were identified as R. crenulata, R. kirilowii, and R. fastigiata. ConclusionITS2 and psbA-trnH barcodes can identify monophyletic groups represented by R. crenulata. However, for non-monophyletic species, it is necessary to collect as many samples as possible and combine them with multiple markers for joint identification. This study discussed the application and challenges of DNA barcodes in Rhodiola under rapid radiation conditions, providing a scientific basis for the rational development and utilization of Rhodiola varieties.

Rapid DNA barcoding‐based fern and lycophyte inventories of protected areas—A pilot study to introduce a simple but effective protocol

AbstractRecording inventories of species conserved in protected areas is a key step to evaluate the effectiveness of Kunming Montreal Global Biodiversity Framework (KM‐GBF) targets, such as the expansion of protected areas. The application of DNA barcoding facilitates the rapid production of enables to obtain rapid inventories with reduced reliance on taxon experts. These inventories aim not only to confirm existing records but also to minimize gaps in our knowledge of the distribution and taxonomy of species targeted for conservation through the implementation of protected areas. This pilot study introduces a simplified DNA barcoding pipeline as a reliable tool for recording fern and lycophyte species occurring in protected areas. The pipeline emphasizes limited and/or short training requirements, reducing the input required from taxon experts and maximizing shared benefits between conservationists and taxonomists. Despite using a single DNA barcoding region, 78% of the accessions were unambiguously identified to the species level. This applied approach not only confirmed previous records but also identified several previously overlooked species, either as newly recorded species conserved in the protected area or as species new to science. The pilot project effectively documented known species diversity and identified gaps in our taxonomic knowledge by discovering previously unknown and locally rare taxa. This rapid assessment enhances productive exchanges between conservation practitioners and taxon experts, with substantial benefits for both parties.

Commentary: brain endothelial GSDMD as a novel target for brain disorders associated with BBB damage

Variations in herb dosage due to species adulteration and dosing inaccuracies can substantially affect clinical safety and efficacy. Accurate species quantification remains challenging, as current methods often yield inconsistent results. This study introduces a novel pyrosequencing-based technique, termed herb molecular quantification (Herb-Q), designed to precisely quantify herbal products. We evaluated its effectiveness using Pinellia ternata and five of its adulterants. Initially, we assessed commonly used DNA barcodes with sequences from a public database, identifying two candidate regions, Maturase K (matK) and internal transcribed spacer 2 (ITS2), for screening specific single nucleotide polymorphism (SNP) loci, allowing for species-specific identification. These loci were validated by amplifying and sequencing genomic material from collected samples. Our validation studies showed that Herb-Q demonstrated excellent linearity, accuracy, repeatability, and detection limits. We established quantitative standard curves with high R2 values (> 0.99) to enable precise species quantification, which were combined with external standards to provide clear and accurate visual quantification results. The average bias in quantifying the tuber of P. ternata was 2.38%, confirming that Herb-Q can accurately identify and quantify herbal product constituents. Moreover, the entire quantification process took less than 4 h. This study presents a novel, rapid method for accurately quantifying species in herbal products and advances the application of DNA barcoding from species identification to quantitative detection.

Molecular quantification of herbs (Herb-Q): a pyrosequencing-based approach and its application in Pinellia ternata

Variations in herb dosage due to species adulteration and dosing inaccuracies can substantially affect clinical safety and efficacy. Accurate species quantification remains challenging, as current methods often yield inconsistent results. This study introduces a novel pyrosequencing-based technique, termed herb molecular quantification (Herb-Q), designed to precisely quantify herbal products. We evaluated its effectiveness using Pinellia ternata and five of its adulterants. Initially, we assessed commonly used DNA barcodes with sequences from a public database, identifying two candidate regions, Maturase K (matK) and internal transcribed spacer 2 (ITS2), for screening specific single nucleotide polymorphism (SNP) loci, allowing for species-specific identification. These loci were validated by amplifying and sequencing genomic material from collected samples. Our validation studies showed that Herb-Q demonstrated excellent linearity, accuracy, repeatability, and detection limits. We established quantitative standard curves with high R2 values (> 0.99) to enable precise species quantification, which were combined with external standards to provide clear and accurate visual quantification results. The average bias in quantifying the tuber of P. ternata was 2.38%, confirming that Herb-Q can accurately identify and quantify herbal product constituents. Moreover, the entire quantification process took less than 4 h. This study presents a novel, rapid method for accurately quantifying species in herbal products and advances the application of DNA barcoding from species identification to quantitative detection.

A Review on Bridging Molecular Biology and Ecological Dynamics through Integrative Approaches in Zoology

The integration of molecular biology with ecological dynamics has emerged as a transformative approach in zoology, enhancing our understanding of biodiversity, ecosystem health, and the adaptive responses of species to environmental changes. This review synthesizes key developments and methodological innovations at the intersection of molecular biology and ecological dynamics, highlighting the application of DNA barcoding, environmental DNA (eDNA) analyses, molecular phylogenetics, and advanced computational models in elucidating complex biological interactions and evolutionary patterns. Significant advancements include the use of high-throughput sequencing technologies and CRISPR-Cas systems that have expanded our ability to explore genetic diversity and manipulate genetic material for conservation purposes. The review discusses the predictive capabilities of integrative models that combine genetic with ecological data, offering insights into species resilience and ecosystem stability under varying environmental scenarios. Challenges in data integration, such as issues of scale, complexity, and the necessity for interdisciplinary cooperation, are critically examined. Technical limitations related to data management and ethical considerations in the use of genetic information are also explored. Looking forward, the review identifies emerging technologies and their potential impacts on ecological and conservation biology, emphasizing the need for policies that support sustainable management and conservation strategies. This review underscores the profound impact of integrating molecular biology with ecological dynamics, which not only enhances our scientific understanding but also provides practical frameworks for addressing global environmental challenges.

Business accounting and information support through the QR- coding system

Business accounting and information support through the QR- coding system

An Analysis of Split-Ring Resonators and Potential Barcode Application

This paper presents an analysis of various split-ring resonator (SRR) configurations coupled with coplanar waveguides (CPW). Three distinct resonant structures are presented, namely, the S-shaped split-ring resonator (S-SRR), the conventional split-ring resonator (SRR), and a pair of SRR. Among the examined resonant structures, it is observed that the S-SRR exhibits the smallest electrical size with the corresponding resonant frequency of 1.37 GHz, while the conventional SRR has the largest electrical size with the corresponding resonant frequency of 3.34 GHz. On the other hand, the resonant frequency of a dual SRR is 2.37 GHz. However, the transmission coefficient of a dual SRR is -11.43 dB, which is higher than S-SRR (-10.02 dB) and SRR (-8.18 dB). Furthermore, this study extends the analysis between a square dual SRR and a circular dual SRR with retained area. The square SRR demonstrates a lower resonance frequency (2.23 GHz) relative to its circular counterpart (2.37 GHz). The comparison also shows that the transmission coefficient of the square configuration is -13.88 dB, which is higher than its circular counterpart (-11.43 dB). The realization of a barcode application is achieved by loading multiple S-shaped split ring resonators (S-SRRs) with varying geometric parameters adjacently onto the transmission line. By individually rotating each of these S-SRRs, it becomes possible to alter the notch magnitude, thereby positioning it within a specific designated range corresponding to a particular code. In the case of a configuration involving rotations of (0°, 45°, 90°, 0°, 45°, 90°), this approach results in the creation of a barcode denoted as "100 010 000 100 011 000". This methodology enables the encoding of information in the form of distinctive resonant responses, providing a versatile and compact means of realizing barcode applications.

Enhancement of Luminescence from Lanthanide Metal–Organic Frameworks by Ytterbium and Calcium Doping: Application to Photonic Barcodes and Fingerprint Detection

AbstractEmission from metal–organic frameworks (MOFs) made from Eu3+ and 1,3,5‐benzenetricarboxylic acid (BTC) is enhanced eightfold by doping with Y3+ and Ca2+ ions. The Ca2+ ions are shown to substitute into the MOFs, and the MOFs structure is shown to be retained at high Y3+ doping levels. The emission enhancement is shown to be associated with variations in the local electric field at the Eu3+ centers in the MOFs. Calculations indicate that the HOMO and LUMO levels vary considerably with both Y3+ doping and with low‐level Ca2+ doping. These then modulates Eu3+ concentration quenching, ligand‐metal energy transfer processes, and the local electric field at the Eu3+ centers, qualitatively accounting for the primary observed features. For UV excitation (250, 295, and 393 nm, respectively), the greatest emission enhancement comes from the doped MOFs with 10% Eu3+, 89% Y3+, and 1% Ca2+. In a photonic barcode application, the doped MOFs are shown to facilitate increased information storage density, and in a fingerprinting application, they are displayed to lead to higher photostability and reduced materials demand.

Application of DNA barcodes in the genetic diversity of hard ticks (Acari: Ixodidae) in Kazakhstan.

Forty-five tick species have been recorded in Kazakhstan. However, their genetic diversity and evolutionary relationships, particularly when compared to ticks in neighbouring countries, remain unclear. In the present study, 148 mitochondrial cytochrome c oxidase subunit I (COI) sequence data from our laboratory and NCBI (National Center for Biotechnology Information; https://www.ncbi.nlm.nih.gov/ ) data were used to address this knowledge gap. Phylogenetic analyses showed that i) Hyalomma anatolicum anatolicum (Koch, 1844) ticks from Jambyl Oblast (southeastern Kazakhstan) and Gansu Province (northwestern China) constituted a newly deviated clade; and ii) Dermacentor reticulatus (Fabricius, 1974) ticks from South Kazakhstan Oblast were closer to those in Romania and Turkey. The network diagram of haplotypes showed that i) the H-1 and H-2 haplotypes of Dermacentor marginatus (Sulzer, 1776) ticks from Zhetisu and Almaty were all newly evolved; and ii) the H-3 haplotypes of Haemaphysalis erinacei (Pavesi, 1884) from Almaty Oblast and Xinjiang Uygur Autonomous Region (northwestern China) were evolved from the H-1 haplotype from Italy. In the future, more COI data from different tick species, especially from Kazakhstan and neighbouring countries, should be employed in the field of tick DNA barcoding.

Molecular characterization and phylogenetic position of the giant deep-sea oyster Neopycnodonte zibrowii Gofas, Salas & Taviani, 2009

The giant deep-sea oyster Neopycnodonte zibrowii Gofas, C. Salas & Taviani, 2009 is a keystone deep-sea habitat builder species. Discovered about fifteen years ago in the Azores, it has been described and assigned to the genus Neopycnodonte Fischer von Waldheim, 1835 based on morphological features. In this study, we generated DNA sequence data for both mitochondrial (COI and 16S) and nuclear (ITS2 and 28S) markers based on the holotype specimen of N. zibrowii to establish a molecular phylogenetic framework for the systematic assessment of this species and to provide a reliable (i.e., holotype-based) reference sequence set for multilocus DNA barcoding approaches. Molecular data provide compelling evidence that the giant deep-sea oyster is a distinct species, rather than a deep-water ecophenotype of Neopycnodonte cochlear (Poli, 1795), with extremely high genetic divergence from any other gryphaeid. Multilocus phylogenetic analyses place the giant deep-sea oyster within the clade “Neopycnodonte/Pycnodonte” with closer affinity to N. cochlear rather than to P. taniguchii Hayami & Kase, 1992, thus supporting its assignment to the genus Neopycnodonte. Relationships within this clade are not well supported because mitochondrial variation is inflated by saturation that eroded phylogenetic signal, implying an old split between taxa within this clade. Finally, the set of reference barcode sequences of N. zibrowii generated in this study will be useful for a wide plethora of barcoding applications in deep-sea biodiversity surveys. Molecular validation of recent records of deep-sea oysters from the Atlantic Ocean and the Mediterranean Sea will be crucial to clarify the distribution of N. zibrowii and assess the phenotypic variation and ecology of this enigmatic species.

Protein nanobarcodes enable single-step multiplexed fluorescence imaging.

Multiplexed cellular imaging typically relies on the sequential application of detection probes, as antibodies or DNA barcodes, which is complex and time-consuming. To address this, we developed here protein nanobarcodes, composed of combinations of epitopes recognized by specific sets of nanobodies. The nanobarcodes are read in a single imaging step, relying on nanobodies conjugated to distinct fluorophores, which enables a precise analysis of large numbers of protein combinations. Fluorescence images from nanobarcodes were used as input images for a deep neural network, which was able to identify proteins with high precision. We thus present an efficient and straightforward protein identification method, which is applicable to relatively complex biological assays. We demonstrate this by a multicell competition assay, in which we successfully used our nanobarcoded proteins together with neurexin and neuroligin isoforms, thereby testing the preferred binding combinations of multiple isoforms, in parallel.

2D Hierarchical Microbarcodes with Expanded Storage Capacity for Optical Multiplex and Information Encryption.

The design of nanosegregated fluorescent tags/barcodes by geometrical patterning with precise dimensions and hierarchies could integrate multilevel optical information within one carrier and enhance microsized barcoding techniques for ultrahigh-density optical data storage and encryption. However, precise control of the spatial distribution in micro/nanosized matrices intrinsically limits the accessible barcoding applications in terms of material design and construction. Here, crystallization forces are leveraged to enable a rapid, programmable molecular packing and rapid epitaxial growth of fluorescent units in 2D via crystallization-driven self-assembly. The fluorescence encoding density, scalability, information storage capacity, and decoding techniques of the robust 2D polymeric barcoding platform are explored systematically. These results provide both a theoretical and an experimental foundation for expanding the fluorescence storage capacity, which is a longstanding challenge in state-of-the-art microbarcoding techniques and establish a generalized and adaptable coding platform for high-throughput analysis and optical multiplexing.